- Open Access
Highly conserved serine residue 40 in HIV-1 p6 regulates capsid processing and virus core assembly
Retrovirology volume 8, Article number: 11 (2011)
The HIV-1 p6 Gag protein regulates the final abscission step of nascent virions from the cell membrane by the action of two late assembly (L-) domains. Although p6 is located within one of the most polymorphic regions of the HIV-1 gag gene, the 52 amino acid peptide binds at least to two cellular budding factors (Tsg101 and ALIX), is a substrate for phosphorylation, ubiquitination, and sumoylation, and mediates the incorporation of the HIV-1 accessory protein Vpr into viral particles. As expected, known functional domains mostly overlap with several conserved residues in p6. In this study, we investigated the importance of the highly conserved serine residue at position 40, which until now has not been assigned to any known function of p6.
Consistently with previous data, we found that mutation of Ser-40 has no effect on ALIX mediated rescue of HIV-1 L-domain mutants. However, the only feasible S40F mutation that preserves the overlapping pol open reading frame (ORF) reduces virus replication in T-cell lines and in human lymphocyte tissue cultivated ex vivo. Most intriguingly, L-domain mediated virus release is not dependent on the integrity of Ser-40. However, the S40F mutation significantly reduces the specific infectivity of released virions. Further, it was observed that mutation of Ser-40 selectively interferes with the cleavage between capsid (CA) and the spacer peptide SP1 in Gag, without affecting cleavage of other Gag products. This deficiency in processing of CA, in consequence, led to an irregular morphology of the virus core and the formation of an electron dense extra core structure. Moreover, the defects induced by the S40F mutation in p6 can be rescued by the A1V mutation in SP1 that generally enhances processing of the CA-SP1 cleavage site.
Overall, these data support a so far unrecognized function of p6 mediated by Ser-40 that occurs independently of the L-domain function, but selectively affects CA maturation and virus core formation, and consequently the infectivity of released virions.
The Gag polyprotein Pr55 of HIV-1 comprises the main structural components that are essential and sufficient for the formation of virus like particles (VLPs). Following synthesis in the cytoplasm, the Gag polyproteins are targeted to the plasma membrane where they assemble into immature budding particles. Concurrent with assembly and release of nascent virions, the Pr55 Gag precursor is cleaved by the autocatalytically activated viral protease (PR), generating the matrix (MA, p17), capsid (CA, p24), nucleocapsid (NC, p7), and the p6 protein. This processing ultimately leads to structural rearrangement of Gag molecules within the virion and the formation of the typical cone shaped core structure, characteristic for a mature infectious particle . MA mediates the plasma membrane targeting of Gag polyproteins and, after cleavage, lines the inner shell of the mature virion. CA forms the conical core shell encasing NC, which regulates packaging and condensation of the viral genome [2–6]. The C-terminal p6 domain of Pr55, the smallest known lentiviral protein, containing 52 amino acids,, comprises a quite complex structural and functional organization and contains two distinct late assembly (L-) domains that regulate efficient separation of assembled virions from the cell surface. L-domains function as docking sites for components of ESCRT (endosomal sorting complex required for transport), cellular multi-protein complexes that are normally involved in the endocytotic recycling of cell surface receptors and in cytokinesis [4, 7–17].
The L-domain activity of p6 is mainly driven by the 7PTAP10 motif that is responsible for the recruitment of the primary budding factor Tsg101 (tumor susceptibility gene 101) to the virus assembly site [15, 18–20]. Another region of p6 involves the residues 36YPLASL41, comprising a cryptic YPXnL-type L-domain, which forms a degenerated version of the YPDL L-domain motif (YPXnL, n = 3) found in equine infectious anemia virus (EIAV). This secondary L-domain in p6 represents a binding site for another cellular budding factor, AIP1/ALIX (ALG-2 interacting protein 1/X, ALIX is used hereafter), a multifunctional ESCRT-associated regulator of intracellular protein transport [13, 21]. In addition to the interaction with cellular ESCRT components, p6 mediates the incorporation of the HIV-1 accessory protein Vpr into virus particles. This incorporation was shown to be dependent on three motifs in p6, the 15FRFG18 motif, the 34ELY36 motif, and the 41LXXLF45 motif [22–24].
Besides these well characterized interactions, p6 contains several highly conserved amino acids, some of which were shown or proposed to undergo posttranslational modification. In this study, we show that mutation of the highly conserved Ser residue in position 40 (Ser-40) leads to an irregular core assembly of the released virions, a reduced infectivity, and, thus, a disturbed virus replication capacity. The results support a novel function of p6 in virus maturation that occurs independently of L-domain function.
Mutation of Ser-40 has no effect on folding of p6
HIV-1 p6 is located within one of the most polymorphic regions of the HIV-1 gag gene, yet the 52 amino acid peptide harbors one of the densest region of protein interacting domains in HIV-1. Figure 1A gives an overview of the previously reported binding domains of cellular (Tsg101, ALIX, ERK-2, SUMO-1, ubiquitin) and viral (Vpr) proteins within p6 from HIV-1NL4-3 and their relationship to the primary and secondary structures . An alignment of consensus sequences, derived from all HIV-1 group M subtypes, revealed that the known functional domains overlap - as expected - with conserved residues in p6.
While the PTAP L-domain is highly conserved, the conservation within the ALIX binding site varies to some extent (Figure 1). The ALIX-binding motif has been defined as (L)[FY]PX1-3LXX[IL] [21, 26, 27] and corresponds in p6 derived from HIV-1NL4-3  to 35LYPLASLRSL44, in which essential residues are in bold. Interestingly, the three amino acid motif 35LYP37 at the start of the binding region is only poorly conserved (Figure 1) while both, Leu-41 and Leu-44, are highly conserved. These two residues together, with the downstream Phe-45, comprise the LXXLF binding domain for the HIV-1 accessory protein Vpr. From previous structural and mutational analysis, it can be concluded that Thr-39 and Ser-40 do not directly participate in the binding of p6 to ALIX [21, 27, 29]. Consistently, Thr-39 is not conserved, while in contrast, Ser-40 is highly conserved among HIV-1 group M isolates, indicating a function other than ALIX binding.
To investigate a potential function of Ser-40 in p6, the residue was mutated in the infectious molecular clone HIV-1NL4-3  and an otherwise isogenic R5-tropic derivative thereof carrying the 005pf135 V3 loop region in Env . In order to obtain replication competent viruses, the mutation was introduced in a way that does not affect the overlapping pol-ORF. In particular, Ser-40 of p6 overlaps in the pol-ORF with the cleavage site between the transframe p6* protein and PR. The only possibility to leave the pol-ORF unaffected was to exchange Ser-40 for Phe, creating the mutant S40F.
Considering this limitation in mutating p6, we wanted proof that the non-conservative S40F exchange does not disturb the secondary structure of p6 in the respective region. To ascertain whether the S40F mutation alters the C-terminal helix of p6, the synthetic (s)p623-52S40F peptide was characterised by 1H NMR spectroscopy . Recently, we solved the structure of sp623-52 by NMR and found that the C-terminal fragment adopts the same structure as the full length molecule sp61-52. Therefore, it was legitimate to analyse the structure of the mutant and compare it with the wt molecule. After complete assignment of the 1H resonances of the NMR spectra of the peptide, NOE cross peaks important for secondary structure identification were identified. The observation of NH i - NH i+1 , NH i - NH i+2 , αH i - NH i+ 2 , αH i - NH i+ 3 , αH i - NH i+ 4 and αH i - βH i + 3 NOEs, which are indicative of helical secondary structure, showed that, similarly to wt sp623-52, sp623-52S40F has a preference for an α-helical structure involving residues Ile-31 - Asp-48 under hydrophobic membranous conditions (50% aqueous trifluorethanol (TFE) solution). Substitution of Ser-40 with Phe did not change the position or number of residues included in the C-terminal helix compared with wt sp623-52, and their structures appear to be similar. This is confirmed from a comparison of plots of the α-proton chemical shifts relative to those of random coil values (Figure 2), which indicates that the substitution slightly stabilizes the region of the C-terminal helix comprised of residues Leu-41 - Leu-44 proximal to Phe-40.
Mutation of Ser-40 in HIV-1 compromises virus replication
The finding that Ser-40 in p6 is highly conserved raises the question whether this amino acid is important for the function of p6 in HIV-1 replication. To investigate this, comprehensive replication studies of HIV-1 carrying mutation of Ser-40 in p6 were conducted in T cells and primary lymphocytes. Purified virus stocks of wt HIV-1NL4-3 and the S40F mutant were generated in 293T cells and normalized for p24 content. First, parallel cultures of CEM T-cells were infected with 20 and 50 ng of input virus, respectively, and samples of culture supernatants collected every other day were analyzed for secretion of virus particles by measuring the virus associated reverse transcriptase (RT) activity. The resulting replication profiles are shown in Figure 3A and 3B. Mutation of Ser-40 resulted in a diminished replication capacity of HIV-1, which is observed as a delayed onset of virus replication (Figure 3A). Furthermore, increase of the input virus to 50 ng resulted in a forward shift of the peak of virus replication. Yet, the overall virus production was still reduced, indicating altogether a compromised replication capacity of the Ser-40 mutant of p6 (Figure 3B). To further evaluate the relevance of Ser-40 in p6, replication studies were conducted in lymphoid cells, derived from human tonsillary tissue, cultivated as aggregate cultures (HLAC) . The HLAC system has been reported to be of comparable value to that of previously described human lymphoid tissue cultures (HLT) where virus replication is studied in tissue blocks [32, 33]. Generally, both HLACs and HLTs, support productive HIV-1 replication independently of the coreceptor tropism and do not require artificial exogenous activation of host cells [32, 33]. Parallel cultures of HLAC were infected with X4-tropic or R5-tropic HIV-1NL4-3 carrying mutation of Ser-40. Samples of culture supernatant were harvested every third day, and secretion of viral particles was determined by measuring the virus associated RT activity. The resulting replication profiles obtained in tissue samples, derived from two different donors for X4-tropic strains, are shown in Figure 3C and 3E, and for R5-tropic variants in Figure 3D and 3F. Consistent with results obtained in CEM cells (Figure 3A, 3B), mutation of Ser-40 resulted in a reduced replication capacity of both X4-tropic and R5-tropic viruses.
Mutation of Ser-40 in p6 does not affect virus release but disturbs CA maturation
To analyze whether the decreased replication capacity of the Ser-40 mutant is due to either a reduction of virus particle production or to a loss of infectivity of the released virions, we first investigated whether Ser-40 is somehow involved in the L-domain mediated assembly and release of virus particles. Virus release kinetics were studied by pulse chase metabolic labeling experiments. Parallel cultures of HeLa cells were transfected with the env-deleted HIV-1NL4-3 subgenomic expression vector pNLenv1 [34, 35], encoding either wt p6 or the S40F mutant. Cells were pulse labeled with [35S]-methionine for 15 minutes and chased for up to 4 hours. At each time point indicated, samples of cells were harvested, and VLPs released into the supernatants were collected by centrifugation and processed for immunoprecipitation with Gag-specific antibodies. Immunoprecipitated proteins were separated by SDS-PAGE and analyzed by fluorography (Figure 4A). The amounts of Gag recovered from virus and cell lysates were quantified by image analysis. The release of VLPs was calculated as percentage of Gag proteins found in the virus fraction, relative to the total amount of Gag recovered from virus and cell fractions and plotted as a function of time (Figure 4B). Clearly, virus release was not affected by mutation of Ser-40. However, quantification of the CA processing products p25 and p24 revealed a significant reduction in the processing rate of p25 to p24 for the S40F mutant (Figure 4C). Thus, Ser-40 somehow regulates the proteolytic maturation of the CA-SP1 Gag processing intermediate, without affecting the overall efficiency of virus release.
S40F mutation reduces specific infectivity of the virions
Having shown that mutation of Ser-40 in p6 does not affect L-domain mediated virus release but disturbs maturation of CA, we analyzed the role of this Ser residue in maturation of progeny virions. To measure the specific infectivity of the mutant, HeLa TZM-bl cells were infected with individual virus stocks standardized for p24 content and infectivity was determined by β-galactosidase assay. Consistent with virus replication data, mutation of Ser-40 reduced the infectivity by approximately 6-10 fold when compared to the wt virus (Figure 5).
Mutation of Ser-40 has no effect on ALIX mediated virus release
Ser-40 residue is located within the previously identified 36YPLASL41 ALIX binding sequence, although shown not to be involved in the interaction with ALIX directly [21, 29]. It was further reported that mutation of either Leu-41 or Leu-44 directly adjacent to Ser-40 exhibit a similar phenotype to that observed for the S40F mutant in terms of CA processing . Therefore, it was legitimate to investigate whether mutation of Ser-40 affects the functional interaction between ALIX and p6. We first examined the ability of ALIX to rescue the release of an HIV-1ΔPTAP L-domain mutant by overexpression in the absence of the Ser-40 [21, 37]. The S40F mutation was cloned into a variant of HIV-1NL4-3 where the PTAP motif was replaced by LIRL (HIV-1ΔPTAP ). 293T cells were cotransfected with plasmids encoding ALIX and either HIV-1ΔPTAP or HIV-1ΔPTAP/S40F. As control, cells were cotransfected with HIV-1ΔPTAP/ΔYP, where the ALIX binding site YPX3L was replaced by SRX3L. As previously reported, this mutation completely abrogates ALIX mediated rescue of release of HIV-1ΔPTAP variants . Gag processing and release of infectious virions were determined 24 hours post transfection by Western blot and single round infection of TZM-bl cells. The results shown in Figure 6A demonstrate that in the presence of the intact ALIX binding site in p6, overexpression of ALIX dramatically stimulated virus release by more than 10-fold as shown by Western blot and single round infection of TZM-bl cells (Figure 6A, lanes 1 and 2). Consistent with previous results [21, 27], the mutation of the minimal ALIX binding motif further reduced virus release even below the residual budding of an HIV-1ΔPTAP mutant (Figure 6A, lane 5), and completely prevents rescue of the HIV-1ΔPTAP variant by overexpression of ALIX (Figure 6A, lane 6) . Mutation of Ser-40 also reduced the residual release of the HIV-1ΔPTAP mutant (Figure 6A, lane 3). In contrast to the ΔYP mutation, in the presence of the S40F mutation, overexpression of ALIX still efficiently rescued the release of the HIV-1ΔPTAP mutant (Figure 6A, lane 4 upper panel). However, overexpression of ALIX did not restore the infectivity of the S40F mutant virions (Figure 6A, lane 4 lower panel), indicating that the loss of infectivity induced by mutation of Ser-40 occurs independently of the ALIX-p6 interaction.
To further uncouple the phenotype induced by S40F mutation from the underlying L-domain function of the ALIX binding site, we investigated whether ALIX can rescue the infectivity of the S40F mutant in the context of a functional PTAP motif. To this end, 293T cells were co-tranfected with HIV-1 encoding either wt p6 or the S40F mutant and ALIX. Release of infectious virions was determined 24 hours post transfection by single round infection of TZM-bl cells. Consistent with previous results, the S40F mutation reduced the infectivity of released virions by ~5-fold (Figure 6B, 1 and 3). While overexpression of ALIX had no significant influence on the infectivity of wt HIV-1 (Figure 6B, 2), ALIX also could not restore the reduced infectivity of the S40F mutant (Figure 6B, 4).
In order to determine whether the ALIX mediated rescue of the HIV-1ΔPTAP variant of the S40F is still comparable to the control, we measured the requirement of ALIX for HIV-1ΔPTAP release at varying ALIX concentrations. 293T cells were cotransfected with HIV-1ΔPTAP and increasing amounts of ALIX expression plasmids. Subsequent determination of virus release by Western blot (Figure 7A) showed that in the presence of the S40F mutation similar amounts of ALIX were required to stimulate virus release (Figure 7A and 7B). Notably, ALIX substantially improved the processing of CA of the HIV-1ΔPTAP mutant. In contrast, CA processing of the HIV-1ΔPTAP/S40F mutant was further impaired, compared to the HIV-1ΔPTAP mutant, and was not rescued by overexpression of ALIX (Figure 7A). This was further supported by the notion that the infectivity of the S40F mutant virions was substantially reduced and could not be restored by overexpression of ALIX (Figure 7C). Taken together, the data indicate that the interaction of p6 with ALIX is not affected by replacing the conserved Ser-40 by Phe, and the phenotype induced by this mutation occurs independently of the ALIX mediated L-domain function of p6 in this region.
The S40F mutation does neither affect cleavage of Gag products, other than CA, nor incorporation of Env
In order to rule out the possibility that mutation of Ser-40 also affects processing of Gag proteins other than CA, virus preparations were analyzed by Western blotting using antibodies specific for NC or MA. Virus particles prepared by transient transfection of 293T cells were purified by centrifugation through a 20% sucrose cushion and standardized for p24 content by ELISA. Equal amounts of p24 were loaded on SDS-PAGE and analyzed by Western blotting. The amount of MA and NC proteins in S40F mutant viruses was similar to that of wt virions, indicating that maturation of these Gag proteins is not affected by the S40F mutation (Figure 8A). Even with the relatively low resolution of the SDS-PAGE system, the disturbed processing of CA from p25 to p24 was detectable (Figure 8A).
It was shown previously that mutations of p6 in this area, in particular, mutations of Tyr-36 and Leu-41 produce mutants that fail to package Env proteins into virus particles . Since Ser-40 is located directly adjacent to Leu-41, we wanted to exclude that the reduced replication capacity and infectivity of the S40F mutant is due to reduced Env incorporation. To this end, purified virions standardized for p24 content were analyzed by Western blotting using Env specific antibodies. As shown in Figure 8B, the S40F mutation had no influence on Env incorporation into virus particles.
Electron microscopy analysis of p6 S40F mutants
Next, we examined the effects of the S40F mutation on assembly, release, and virion morphology by thin-section electron microscopy (Figure 9). HeLa cells transiently transfected with plasmids encoding HIV-1NL4-3 and mutants thereof were drawn into cellulose capillary tubes 24 hours post transfection. The cellulose capillary tubes retain secreted virions, thereby obviating the need for centrifugation steps that usually affect the native virion structure. In agreement with our biochemical data, accumulation of virions tethered at the cell membrane, a phenotype commonly observed for L-domain mutants in p6, was not observed for the S40F mutant (data not shown). However, and most intriguingly, mutation of Ser-40 in p6 led to the formation of aberrant virus particles, characterized by irregularly shaped viral cores and the formation of closely neighboring electron-dense lateral bodies, as indicated in Figure 9A. These irregularly shaped cores and lateral bodies were not observed in the wt and the ΔYP mutant, again indicating that this phenotype occurs independently of the ALIX-Gag interaction. As we observed a deficiency in CA processing of p25 to p24 in the virions containing the S40F mutation (Figure 9), we investigated whether this defect in virus core assembly is a consequence of imperfect CA processing. A previously characterized CA5 mutant was generated in HIV-1NL4-3 that is incapable of processing p25 to p24 and was shown to exhibit a similar defect of core assembly . Indeed, the phenotype observed for CA5 by analyzing the core structures of this mutant clearly resembles that of the S40F mutant (Figure 9A).
To evaluate quantitatively this morphological phenomenon, cores of 100 - 120 virons were counted and the percentages of irregular core structures relative to wt virions were calculated (Figure 9B). Obviously, mutation of Ser-40 significantly increases the amount of virions containing aberrant, irregularly shaped virus cores. Moreover, the CA5 mutant, in which CA processing is blocked completely, shows the same phenotype as that of the S40F mutant, further supporting the notion that Ser-40 governs the processing of CA by a yet unidentified mechanism.
Defect in CA maturation of the S40F mutant can be rescued by mutation in the CA-SP1 cleavage site
As described above, the S40F mutation increases the ratio of p25 to mature p24. Our data, together with previous results from others, suggest that this disturbed CA processing subsequently leads to an irregular morphology of the virus core and thus, to reduced virus infectivity of the virions. This prompted us to investigate, whether restoring the CA processing by introducing specific mutations into the CA-SP1 cleavage site can rescue the defects in core assembly and infectivity induced by the S40F mutation. It is known that the affinity of the PR to the cleavage site between CA and SP1 is weak compared to other proteolytic cleavage sites in Gag [41, 42]. The A1V mutation in the SP1, which previously was identified to confer resistance to the CA-maturation inhibitor Bevirimat , enhances the affinity of the viral protease to the CA-SP1 cleavage site. Thus, the A1V mutation in SP1 was introduced into the HIV-1NL4-3 backbone in combination with the S40F mutation in p6. Western blot analysis of purified virions revealed that, by introducing the A1V mutation, CA processing is substantially enhanced in both, the wt and the S40F mutant virions (Figure 10A). Furthermore, the A1V/S40F mutant displayed a similar CA processing compared to the wt.
Consequently, we wanted to examine whether this enhanced CA processing affects virus core assembly. Therefore, virion structure of the A1V mutants was analyzed by thin-section electron microscopy. Cores of 100 - 120 virons were counted and the percentages of irregular core structures relative to wt virions were calculated (Figure 10B). The S40F mutation again substantially increases the amount of virions containing aberrant, irregularly shaped virus cores. The A1V mutation had only marginal effects on the core morphology of wt HIV-1. However, in the case of the S40F mutant, introducing the A1V mutation largely improves virus core assembly (Figure 10B).
Since enhancing the CA processing rate rescues the defect of viral core assembly, we subsequently wanted to analyze whether this improved core formation also affects the infectivity of the virions. To measure the specific infectivity, HeLa TZM-bl cells were infected with individual virus stocks standardized for p24 content and infectivity was determined by β-galactosidase assay. The A1V mutation alone enhances the specific infectivity of the virons by 4-fold (Figure 10C). Introducing the A1V mutation enhances the specific infectivity of the virions of the otherwise attenuated S40F mutant to almost wt levels (Figure 10C), indicating that the deficiency in CA processing is the major determinant for the reduced infectivity of the S40F mutant.
In this study we demonstrate that mutation of the highly conserved Ser-40 interferes with Gag processing and virus core formation. Although Ser-40 in HIV-1 p6 is highly conserved among HIV-1 isolates, it is not involved in any of the functional motifs described so far, neither the ALIX nor the Vpr binding site. Therefore, it was legitimate to speculate that Ser-40 is involved in another, until now unrecognized function of p6. However, it should be noted that the position of Ser-40 in the nucleotide sequence of HIV-1 overlaps with the p6*/PR cleavage site in the overlapping pol-ORF, which limits the probability of mutations in this respective area and might contribute to the high conservation of this amino acid. Nevertheless, our findings of a compromised replication capacity and reduced infectivity of the Ser-40 mutant viruses support the assumption that Ser-40 has an important function directly associated with p6.
Notably, virus release kinetic was not reduced for the S40F mutant providing first evidence that the L-domain function of p6 is not affected by mutation of Ser-40. This was supported further by the observation that the ability of ALIX to rescue HIV-1ΔPTAP L-domain mutant viruses was not influenced by the S40F mutation. Although Ser-40 is located within the ALIX binding region in p6, previous structural investigations indicated that Ser-40 itself does not participate in the binding of p6 to ALIX [21, 27, 29]. The fact that the Ser-40 mutant was still fully active in terms of L-domain function further supports the notion, that the mutation introduced into p6 did not disturb the overall structure of the molecule in this respective region. In consistency, structural calculations indicated that the non-conservative exchange of Ser-40 to Phe does not change the ability of the molecule to adopt a helical structure. In fact, the C-terminal α-helix is conserved in the S40F mutant, as it was established by NMR studies of the C-terminal peptides sp623-52.
Yet, as shown previously, the phenotypes induced by mutations in the ALIX binding site are depending on the type of amino acid that is mutated . Mutation of the 35LYP37 sequences in the ALIX binding site reduces release and infectivity of HIV-1 virions, which otherwise exhibit normal Gag processing [27, 36]. In contrast, mutations of Leu-41 and Leu-44 have no impact on virus release but increase the ratio of p25 to mature p24, similar to the phenotype we observed for mutation of Ser-40 . Unlike the typical phenotype of L-domain mutants, these mutants interfere somehow specifically with the final step in maturation of CA. It is currently not clear, whether this phenotype is in some way associated with the ALIX-p6 interaction. While mutations of Leu-41 and Leu-45 disrupt binding to ALIX, mutation of Ser-40 apparently has no influence on this interaction. Thus, it might also be possible, that this area in p6 harbors another function that, independent of L-domain activity, requires a so far unrecognized cellular interaction partner.
Maturation of the Gag processing intermediate p25 to mature CA p24, e. g. the cleavage of the CA-SP1 junction by the PR, appears to be one of the last steps of Gag processing [41, 42]. It was previously demonstrated that mutating the junction between CA and SP1, in order to block cleavage of p25, leads to the production of noninfectious viral particles with aberrant core morphology . In addition, treatment of virus producing cells with 3-O-(3'-3'dimethylsuccinyl) betulinic acid (BVM, Bevirimat, also known as PA-457 or DSB), a specific inhibitor that blocks PR-mediated cleavage between CA and SP1, disturbed viral core formation . Intriguingly, mutation of Ser-40 leads to an almost identical aberrant virus core morphology as shown previously for CA5 mutants. Both are characterized by misshapen core structures and the formation of an electron dense lateral body near the viral membrane. Previous studies already indicated that maturation of HIV-1 virions, leading to the typical cone shaped cores, is regulated by the sequential, and highly ordered proteolytic cleavage of Gag . Apparently, the last step of Gag processing - the cleavage of the CA-SP1 junction - is required for capsid condensation. However, pulse chase data indicate that the kinetic of cleavage of the CA-SP1 junction is delayed, but not completely blocked inasmuch as the mature CA accumulates over time. This indicates a rather dynamic process in which the mutation of Ser-40 somehow delays the kinetic of CA maturation. This phenomenon correlates with deficiencies in CA processing, infectivity, and core morphology.
Recently published results from Müller et al. demonstrate that even low amounts of Gag processing intermediates interfere with HIV particle maturation in a trans-dominant manner, with the final cleavage between p24 and SP1 being of particular importance . This explains why the rather subtle effect on CA maturation detected for Ser-40 mutants by Western blotting and pulse chase analysis results in a substantial reduction of virus core formation.
Interestingly, the effect of the S40F mutation appears to be specific for the CA-SP1 cleavage inasmuch as i) no other Gag processing deficiency could be detected (Figure 6) and ii) enhancing CA processing by introducing the A1V mutation could restore the deficient core formation, and, consequently, enhance infectivity. Thus, it can be concluded that Ser-40 somehow regulates the cleavage of the CA-SP1 junction and the subsequent capsid condensation.
The molecular mechanism behind how Ser-40 regulates the processing of Gag, in particular the cleavage of the CA-SP1 junction, is still elusive so far. The previously described defects in Gag processing commonly observed for L-domain mutants are believed to be linked to the overall process of virus budding inasmuch as PR activation and subsequent Gag processing occur concomitantly with and shortly after release of virus particles [45, 46]. In the case of Ser-40, this can be excluded, as the mutant S40F exhibits wt budding. However, Ser-40 in p6 and the CA-SP1 junction are separated by 123 amino acids in Pr55 and it remains elusive so far, how both proteins can affect each other, either in the context of Pr55 or after Gag processing. Our NMR experiments demonstrated that the C-terminal structure of p6 is not influenced by the S40F mutation. Therefore, one possibility of the effect observed would be that the mutation affects the Gag structure prior to initiation of Gag processing, thereby reducing the cleavage efficiency of the weakest cleavage site in Pr55. A prerequisite for this scenario would be that p6 represents a structured Gag domain and thus influence the folding the Pr55 polyprotein. Even though the 283 residue N-terminal part of HIV-1 Gag including MA and CA has been solved by NMR , the structure of the complete Pr55 has not been determined hitherto. Although S40F does not appear to affect the folding of the mature p6 protein, we can not exclude that this mutation indeed affect the overall structure of the PR55 polyprotein, which in turn would reduce the processing efficiency, a phenotype we clearly observed for the S40F mutant as a novel function of p6.
Currently, there is no evidence of an intra-molecular interaction between these domains in the Pr55 polyprotein. The p6 domain of the Pr55 represents a docking site of several cellular and viral factors. Thus, since an intramolecular interaction between CA and p6 appears to be unlikely, it is conceivable to hypothesize that p6 harbors another interaction domain of a yet unknown factor that, independently of the L-domains, regulates processing of CA.
Overall, these data support a so far unrecognized function of p6 that occurs independently of the L-domain function, does not affect virus release, but selectively affects CA maturation, virus core formation, and thus, infectivity.
Peptide synthesis and purification
The synthesis, purification and molecular characterization of p6 and the related fragments derived from HIV-1NL4-3 have been described in detail previously .
2D1H Total Correlation Spectroscopy (TOCSY), Correlation Spectroscopy (COSY) and Nuclear Overhauser enhancement spectroscopy (NOESY) NMR experiments were performed at 600.13 MHz on a Bruker Avance 600 MHz instrument equipped with an UltraShield Plus magnet and a triple resonance cryoprobe with gradient unit. Individual samples were dissolved in 600 μl 50% aqueous TFE-d2 at concentrations between 1-2 mM. The 2D NMR experiments were performed at 300 K without spinning with mixing times of 110 ms for the TOCSY experiments and 250 ms for the NOESY experiments, respectively. Efficient suppression of the water signal was achieved with application of excitation sculpting in the 1D 1H and the 2D 1H TOCSY and NOESY NMR experiments. 1H signal assignments of the NMR spectra were achieved by identification of the individual spin systems in the 2D 1H TOCSY spectra, combined with observations of sequence-specific short-distance crosspeaks (Hα-HN i, i+1) in the 2D 1H-1H NOESY spectra [48, 49]. Readily recognizable spin systems were used as starting points for correlation of the individual spin systems observed in the TOCSY and NOESY spectra with individual residues in the peptide sequences. Acquisition of data, processing and spectral analysis were performed with Bruker Topspin 1.3 software.
Antibody specific for FLAG was obtained from Sigma, the ribosomal P antigen specific antiserum from Immunovision Inc., the CA specific antiserum from Seramun. The p6 specific antibody was described earlier . The anti-mouse, anti-rabbit, and anti-human IgG antibodies coupled to horseradish peroxidase (HRP) were obtained from Amersham.
Amino acid exchanges at Ser-40 in p6 were introduced by site-directed mutagenesis using oligonucleotides containing the indicated changes (S40F, ΔYP, and ΔPTAP) and the Quick Change® site directed mutagenesis kit (Stratagene). The mutations were introduced in the X4-tropic HIV-1NL4-3 infectious molecular clone  and isogenic R5-tropic derivative thereof . In order to avoid taking biosafety measures, the mutations were also introduced in two HIV-1NL4-3 based subgenomic expression vectors giving rise to noninfectious VLPs: the pNLenv, in which env was deleted , and a an HIV-1 expression construct that carries a primer binding site deletion, as well as two point mutations in the active site of the RT coding region (pΔR ). All introduced mutations did not lead to mutations in the overlapping pol-ORF.
HeLa SS6, HeLa TZM-bl and 293T cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% (v/v) inactivated fetal calf serum (FCS), 2 mM L-glutamine, 100 U/ml penicillin and 100 μg/ml streptomycin. CEM cells were maintained in RPMI 1640 supplemented with 10% (v/v) inactivated FCS, 2 mM L-glutamine, 100 U/ml penicillin and 100 μg/ml streptomycin. All media and compounds were provided by Gibco.
Preparation and cultivation of primary cells
Human tonsils, removed during routine tonsillectomy, were received a few hours after excision from the Olgahospital, Stuttgart, Germany, prepared and infected as described earlier [32, 33]. After washing the tonsils, human lymphocyte aggregate cultures (HLAC) were prepared by dividing the tonsils into tissue blocks of 2-3 mm and grinding the tissue through the sieve of a cell strainer (70 μm, BD Falcon) with a syringe plunger. Cells were seeded in a 96 well plate at a concentration of 2 × 106 cells per well. HLACs were cultured in RPMI 1640 supplemented with 15% (v/v) inactivated FCS, 2 mM L-glutamine, 100 U/ml penicillin and 100 μg/ml streptomycin, 2.5 μg/ml Fungizone, 1 mM sodium pyruvate, 1% (v/v) MEM non-essential amino acid solution and 50 μg/ml gentamicin.
Western blot for protein analysis
HeLa SS6 cells were transiently transfected with the appropriate DNA using Lipofectamine 2000™ (Invitrogen) according to the manufacturer's protocol. For ALIX cotransfection, 293T cells were transfected with equal amounts of both DNAs and cells were harvested 24 h post transfection. Cells were lysed in cold RIPA buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1% Nonidet P-40, 0.5% sodium deoxycholat, 0.1% Na-SDS, 5 mM EDTA, DNase, 1 mM PMSF and complete protease inhibitor cocktail (Boehringer Mannheim)), and the lysates were cleared by centrifugation at 16000 × g and 4°C for 10 min. RIPA-soluble proteins and VLPs were separated in 10% SDS/PAA gels, according to Laemmli , transferred onto PVDF membranes (GE Healthcare) and probed with specific antibodies, followed by enhanced chemiluminescence detection. For internal controls, blots were stripped and re-incubated with the appropriate antibody.
Metabolic labeling and immunoprecipitation
For pulse chase experiments, adherent cultures of transfected HeLa SS6 cells were washed once with PBS and starved for 30 min in methionine-free, serum-free RPMI 1640. Cells were pulse-labeled for 15 min with [35S]-methionine (3 mCi/ml; Amersham Life Sciences) and chased for up to 4 h while shaking at 37°C in D-MEM, supplemented with 10% FCS and 10 mM methionine. At the indicated time points, cells and supernatants were collected by centrifugation for 1 min at 16000 × g. Virions were pelleted through a 20% (w/v) sucrose cushion and lysed in Triton wash buffer (50 mM Tris-HCl pH 7.4, 300 mM NaCl, 0.1% Triton X-100, 1 mM PMSF). Cells were lysed in RIPA buffer as described above, containing additionally 5 mM N-ethylmaleimide and 20 μM carbobenzoxyl-Leu-Leu-leucinal (zLLL; Sigma). Gag proteins from precleared cell lysates and lysed VLPs were recovered by immunoprecipitation using a mixture of polyclonal rabbit anti-p6 and anti-p24 antibodies prebound to protein G-Sepharose (GE Healthcare). Samples were separated by SDS-PAGE on a 10% (w/v) acryl amide ProSieve gel (Cambrex Bioscience), backed with Gel Bond film (FMC Bioproducts). Following fixation for 1 h in 50% methanol and 10% acetic acid, gels were rinsed with water, soaked in 1 M sodium salicylic acid solution with 10% glycerol for 5 h and dried. Radioactivity in dried gels was quantified using AIDA imaging software (Raytest).
Virus containing cell culture supernatant was harvested after 48 h and, after removal of residual cells by centrifugation, passed through a 0.45 μm pore-size filter. Virus was pelleted through 20% (w/v) sucrose (16000 × g, 4°C, 90 min). Virus stocks were normalized for p24 content as quantified by a enzyme-linked immunosorbent assay (ELISA, Aalto, Dublin, Ireland) and aliquots were stored at -80°C.
Infection of cells
For infection of T cell cultures, 1 × 107 cells were incubated with 20 or 50 ng of p24, respectively, and supernatant was collected every second day post infection. Virus replication was assessed by quantification of the virus-associated RT activity by [32P]-TTP incorporation using an oligo(dT)-poly(A) template as described . For testing each virus in the HLAC from one donor, 1 ng of p24 was applied to 2 × 106 cells in 96 well format, and virus replication was assessed, as described for T cell cultures, every third day post infection.
Viral infectivity assay
HeLa TZM-bl cells were seeded in 96 well format (4000 cells per well) and infected with standardized amount of p24. The next day, fresh medium with 100 μg/ml dextran sulphate was added to prevent further spread of virus infection, and cells were incubated for further two days. Infection was detected using a galactosidase screen kit from Tropix as recommended by the manufacturer. β-Galactosidase activity was quantified as relative light units per second using an Orion Microplate Luminometer (Berthold).
Transmission electron microscopy (TEM)
Transfected HeLa SS6 cells were processed for transmission electron microscopy in the following way: 24 h post transfection, cells were placed in cellulose capillary tubes , cultivated for one more day, then fixed in 2.5% glutaraldehyde for 1 h at 37°C and stored for further preparation at 4°C. Tubes were collected by centrifugation and sealed by immersion in low-melting-point agarose. The samples were post fixed with OsO4 (1% in distilled water, 1 h), tannic acid (0.1% in Hepes 0,05 M, 30 min) and uranyl acetate (1% in distilled water, 2 h) followed by stepwise dehydration in a graded ethanol series and embedding in epon resin, which was subsequently polymerized. Thin sections were prepared with an ultramicrotome (Ultracut S; Leica, Wetzlar, Germany) and counterstained with uranyl acetate and lead citrate. The sections were examined using a TEM 902 (Carl Zeiss SMT AG) at 80 kV, and the images were digitized using a slow-scan charge-coupled-device camera (Pro Scan; Scheuring, Germany). The evaluation of the capsid morphology was performed by using these images or directly on the screen.
Wills JW, Craven RC: Form, function, and use of retroviral gag proteins. Aids. 1991, 5 (6): 639-654. 10.1097/00002030-199106000-00002.
Turner BG, Summers MF: Structural biology of HIV. J Mol Biol. 1999, 285 (1): 1-32. 10.1006/jmbi.1998.2354.
Kräusslich HG, Welker R: Intracellular transport of retroviral capsid components. Curr Top Microbiol Immunol. 1996, 214: 25-63.
Vogt VM: Ubiquitin in retrovirus assembly: actor or bystander?. Proc Natl Acad Sci USA. 2000, 97 (24): 12945-12947. 10.1073/pnas.97.24.12945.
Swanstrom R, Wills JW: Retroviral gene expression: synthesis, processing, and assembly of viral proteins. Retroviruses. Edited by: Coffin JM, Hughes SH. 1997, Varmus HE: Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y, 263-334.
Vogt VM: Retroviral virions and genomes. Retroviruses. Edited by: Coffin JM, Hughes SH. 1997, Varmus HE: Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y, 27-70.
Pornillos O, Garrus JE, Sundquist WI: Mechanisms of enveloped RNA virus budding. Trends Cell Biol. 2002, 12 (12): 569-579. 10.1016/S0962-8924(02)02402-9.
Göttlinger HG, Dorfman T, Sodroski JG, Haseltine WA: Effect of mutations affecting the p6 gag protein on human immunodeficiency virus particle release. Proc Natl Acad Sci USA. 1991, 88 (8): 3195-3199.
Freed EO: Viral late domains. J Virol. 2002, 76 (10): 4679-4687. 10.1128/JVI.76.10.4679-4687.2002.
Greene WC, Peterlin BM: Charting HIV's remarkable voyage through the cell: Basic science as a passport to future therapy. Nat Med. 2002, 8 (7): 673-680. 10.1038/nm0702-673.
Perez OD, Nolan GP: Resistance is futile: assimilation of cellular machinery by HIV-1. Immunity. 2001, 15 (5): 687-690. 10.1016/S1074-7613(01)00238-2.
Luban J: HIV-1 and Ebola virus: the getaway driver nabbed. Nat Med. 2001, 7 (12): 1278-1280. 10.1038/nm1201-1278.
Strack B, Calistri A, Craig S, Popova E, Göttlinger HG: AIP1/ALIX is a binding partner for HIV-1 p6 and EIAV p9 functioning in virus budding. Cell. 2003, 114 (6): 689-699. 10.1016/S0092-8674(03)00653-6.
Carlton JG, Martin-Serrano J: Parallels between cytokinesis and retroviral budding: a role for the ESCRT machinery. Science. 2007, 316 (5833): 1908-1912. 10.1126/science.1143422.
Martin-Serrano J, Zang T, Bieniasz PD: HIV-1 and Ebola virus encode small peptide motifs that recruit Tsg101 to sites of particle assembly to facilitate egress. Nat Med. 2001, 7 (12): 1313-1319. 10.1038/nm1201-1313.
von Schwedler UK, Stuchell M, Müller B, Ward DM, Chung HY, Morita E, Wang HE, Davis T, He GP, Cimbora DM, et al: The protein network of HIV budding. Cell. 2003, 114 (6): 701-713. 10.1016/S0092-8674(03)00714-1.
Pornillos O, Alam SL, Rich RL, Myszka DG, Davis DR, Sundquist WI: Structure and functional interactions of the Tsg101 UEV domain. Embo J. 2002, 21 (10): 2397-2406. 10.1093/emboj/21.10.2397.
Garrus JE, von Schwedler UK, Pornillos OW, Morham SG, Zavitz KH, Wang HE, Wettstein DA, Stray KM, Cote M, Rich RL, et al: Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding. Cell. 2001, 107 (1): 55-65. 10.1016/S0092-8674(01)00506-2.
Myers EL, Allen JF: Tsg101, an inactive homologue of ubiquitin ligase e2, interacts specifically with human immunodeficiency virus type 2 Gag polyprotein and results in increased levels of ubiquitinated Gag. J Virol. 2002, 76 (22): 11226-11235. 10.1128/JVI.76.22.11226-11235.2002.
VerPlank L, Bouamr F, LaGrassa TJ, Agresta B, Kikonyogo A, Leis J, Carter CA: Tsg101, a homologue of ubiquitin-conjugating (E2) enzymes, binds the L domain in HIV type 1 Pr55Gag. Proc Natl Acad Sci USA. 2001, 98 (14): 7724-7729. 10.1073/pnas.131059198.
Fisher RD, Chung HY, Zhai Q, Robinson H, Sundquist WI, Hill CP: Structural and biochemical studies of ALIX/AIP1 and its role in retrovirus budding. Cell. 2007, 128 (5): 841-852. 10.1016/j.cell.2007.01.035.
Kondo E, Göttlinger HG: A conserved LXXLF sequence is the major determinant in p6gag required for the incorporation of human immunodeficiency virus type 1 Vpr. J Virol. 1996, 70 (1): 159-164.
Salgado GF, Marquant R, Vogel A, Alves ID, Feller SE, Morellet N, Bouaziz S: Structural studies of HIV-1 Gag p6ct and its interaction with Vpr determined by solution nuclear magnetic resonance. Biochemistry. 2009, 48 (11): 2355-2367. 10.1021/bi801794v.
Zhu H, Jian H, Zhao LJ: Identification of the 15FRFG domain in HIV-1 Gag p6 essential for Vpr packaging into the virion. Retrovirology. 2004, 1: 26-10.1186/1742-4690-1-26.
Fossen T, Wray V, Bruns K, Rachmat J, Henklein P, Tessmer U, Maczurek A, Klinger P, Schubert U: Solution structure of the human immunodeficiency virus type 1 p6 protein. J Biol Chem. 2005, 280 (52): 42515-42527. 10.1074/jbc.M507375200.
Munshi UM, Kim J, Nagashima K, Hurley JH, Freed EO: An Alix fragment potently inhibits HIV-1 budding: characterization of binding to retroviral YPXL late domains. J Biol Chem. 2007, 282 (6): 3847-3855. 10.1074/jbc.M607489200.
Zhai Q, Fisher RD, Chung HY, Myszka DG, Sundquist WI, Hill CP: Structural and functional studies of ALIX interactions with YPXnL late domains of HIV-1 and EIAV. Nat Struct Mol Biol. 2008, 15 (1): 43-49. 10.1038/nsmb1319.
Adachi A, Gendelman HE, Koenig S, Folks T, Willey R, Rabson A, Martin MA: Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone. J Virol. 1986, 59 (2): 284-291.
Lazert C, Chazal N, Briant L, Gerlier D, Cortay JC: Refined study of the interaction between HIV-1 p6 late domain and ALIX. Retrovirology. 2008, 5: 39-10.1186/1742-4690-5-39.
Papkalla A, Münch J, Otto C, Kirchhoff F: Nef enhances human immunodeficiency virus type 1 infectivity and replication independently of viral coreceptor tropism. J Virol. 2002, 76 (16): 8455-8459. 10.1128/JVI.76.16.8455-8459.2002.
Eckstein DA, Penn ML, Korin YD, Scripture-Adams DD, Zack JA, Kreisberg JF, Roederer M, Sherman MP, Chin PS, Goldsmith MA: HIV-1 actively replicates in naive CD4+ T cells residing within human lymphoid tissues. Immunity. 2001, 15 (4): 671-682. 10.1016/S1074-7613(01)00217-5.
Glushakova S, Baibakov B, Margolis LB, Zimmerberg J: Infection of human tonsil histocultures: a model for HIV pathogenesis. Nat Med. 1995, 1 (12): 1320-1322. 10.1038/nm1295-1320.
Glushakova S, Baibakov B, Zimmerberg J, Margolis LB: Experimental HIV infection of human lymphoid tissue: correlation of CD4+ T cell depletion and virus syncytium-inducing/non-syncytium-inducing phenotype in histocultures inoculated with laboratory strains and patient isolates of HIV type 1. AIDS Res Hum Retroviruses. 1997, 13 (6): 461-471. 10.1089/aid.1997.13.461.
Clavel F, Hoggan MD, Willey RL, Strebel K, Martin MA, Repaske R: Genetic recombination of human immunodeficiency virus. J Virol. 1989, 63 (3): 1455-1459.
Maldarelli F, Martin MA, Strebel K: Identification of posttranscriptionally active inhibitory sequences in human immunodeficiency virus type 1 RNA: novel level of gene regulation. J Virol. 1991, 65 (11): 5732-5743.
Fujii K, Munshi UM, Ablan SD, Demirov DG, Soheilian F, Nagashima K, Stephen AG, Fisher RJ, Freed EO: Functional role of Alix in HIV-1 replication. Virology. 2009, 391 (2): 284-292. 10.1016/j.virol.2009.06.016.
Usami Y, Popov S, Göttlinger HG: Potent rescue of human immunodeficiency virus type 1 late domain mutants by ALIX/AIP1 depends on its CHMP4 binding site. J Virol. 2007, 81 (12): 6614-6622. 10.1128/JVI.00314-07.
Huang M, Orenstein JM, Martin MA, Freed EO: p6Gag is required for particle production from full-length human immunodeficiency virus type 1 molecular clones expressing protease. J Virol. 1995, 69 (11): 6810-6818.
Ott DE, Chertova EN, Busch LK, Coren LV, Gagliardi TD, Johnson DG: Mutational analysis of the hydrophobic tail of the human immunodeficiency virus type 1 p6Gag protein produces a mutant that fails to package its envelope protein. J Virol. 1999, 73 (1): 19-28.
Wiegers K, Rutter G, Kottler H, Tessmer U, Hohenberg H, Kräusslich HG: Sequential steps in human immunodeficiency virus particle maturation revealed by alterations of individual Gag polyprotein cleavage sites. J Virol. 1998, 72 (4): 2846-2854.
Pettit SC, Moody MD, Wehbie RS, Kaplan AH, Nantermet PV, Klein CA, Swanstrom R: The p2 domain of human immunodeficiency virus type 1 Gag regulates sequential proteolytic processing and is required to produce fully infectious virions. J Virol. 1994, 68 (12): 8017-8027.
Pettit SC, Henderson GJ, Schiffer CA, Swanstrom R: Replacement of the P1 amino acid of human immunodeficiency virus type 1 Gag processing sites can inhibit or enhance the rate of cleavage by the viral protease. J Virol. 2002, 76 (20): 10226-10233. 10.1128/JVI.76.20.10226-10233.2002.
Li F, Goila-Gaur R, Salzwedel K, Kilgore NR, Reddick M, Matallana C, Castillo A, Zoumplis D, Martin DE, Orenstein JM, et al: PA-457: a potent HIV inhibitor that disrupts core condensation by targeting a late step in Gag processing. Proc Natl Acad Sci USA. 2003, 100 (23): 13555-13560. 10.1073/pnas.2234683100.
Müller B, Anders M, Akiyama H, Welsch S, Glass B, Nikovics K, Clavel F, Tervo HM, Keppler OT, Kräusslich HG: HIV-1 Gag processing intermediates trans-dominantly interfere with HIV-1 infectivity. J Biol Chem. 2009, 284 (43): 29692-29703.
Kaplan AH, Swanstrom R: Human immunodeficiency virus type 1 Gag proteins are processed in two cellular compartments. Proc Natl Acad Sci USA. 1991, 88 (10): 4528-4532. 10.1073/pnas.88.10.4528.
Kaplan AH, Manchester M, Swanstrom R: The activity of the protease of human immunodeficiency virus type 1 is initiated at the membrane of infected cells before the release of viral proteins and is required for release to occur with maximum efficiency. J Virol. 1994, 68 (10): 6782-6786.
Tang C, Ndassa Y, Summers MF: Structure of the N-terminal 283-residue fragment of the immature HIV-1 Gag polyprotein. Nat Struct Biol. 2002, 9 (7): 537-543.
Bruns K, Fossen T, Wray V, Henklein P, Tessmer U, Schubert U: Structural characterization of the HIV-1 Vpr N terminus: evidence of cis/trans-proline isomerism. J Biol Chem. 2003, 278 (44): 43188-43201. 10.1074/jbc.M305413200.
Wüthrich K: NMR of Proteins and Nucleic Acids. 1986, New York: John Wiley & Sons, Inc
Schubert U, Clouse KA, Strebel K: Augmentation of virus secretion by the human immunodeficiency virus type 1 Vpu protein is cell type independent and occurs in cultured human primary macrophages and lymphocytes. J Virol. 1995, 69 (12): 7699-7711.
Gottwein E, Kräusslich HG: Analysis of human immunodeficiency virus type 1 Gag ubiquitination. J Virol. 2005, 79 (14): 9134-9144. 10.1128/JVI.79.14.9134-9144.2005.
Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970, 227 (5259): 680-685. 10.1038/227680a0.
Willey RL, Smith DH, Lasky LA, Theodore TS, Earl PL, Moss B, Capon DJ, Martin MA: In vitro mutagenesis identifies a region within the envelope gene of the human immunodeficiency virus that is critical for infectivity. J Virol. 1988, 62 (1): 139-147.
Hohenberg H, Mannweiler K, Müller M: High-pressure freezing of cell suspensions in cellulose capillary tubes. J Microsc. 1994, 175 (Pt 1): 34-43.
Hemonnot B, Cartier C, Gay B, Rebuffat S, Bardy M, Devaux C, Boyer V, Briant L: The host cell MAP kinase ERK-2 regulates viral assembly and release by phosphorylating the p6gag protein of HIV-1. J Biol Chem. 2004, 279 (31): 32426-32434. 10.1074/jbc.M313137200.
Ott DE, Coren LV, Copeland TD, Kane BP, Johnson DG, Sowder RC, Yoshinaka Y, Oroszlan S, Arthur LO, Henderson LE: Ubiquitin is covalently attached to the p6Gag proteins of human immunodeficiency virus type 1 and simian immunodeficiency virus and to the p12Gag protein of Moloney murine leukemia virus. J Virol. 1998, 72 (4): 2962-2968.
Gurer C, Berthoux L, Luban J: Covalent modification of human immunodeficiency virus type 1 p6 by SUMO-1. J Virol. 2005, 79 (2): 910-917. 10.1128/JVI.79.2.910-917.2005.
Demirov DG, Ono A, Orenstein JM, Freed EO: Overexpression of the N-terminal domain of TSG101 inhibits HIV-1 budding by blocking late domain function. Proc Natl Acad Sci USA. 2002, 99 (2): 955-960. 10.1073/pnas.032511899.
We thank Dr. Henning Heumann and the surgical staff of the Olgahospital, Stuttgart, for generous assistance in obtaining post-tonsillectomy samples, Raymond Sowder for HPLC purification, and Victor Wray for critical reading of the manuscript. This work was supported by a grant IE-S08T06 from the German Human Genome Research Project, by grants SFB 643-A1, SCHU1125/3, and SCHU 1125/5, from the German Research Council to US.
The authors declare that they have no competing interests.
US designed the study. LN, SH, FH, PR, KS, NS and JV performed virus replications, infectivity as well as Western blot and pulse chase analysis of virions. SMØS and TF performed NMR studies, PH synthesized the peptides and DEO supplied essential material. NB and GH did the electron microscopy. JV and US wrote the manuscript. All authors read and approved the final manuscript.
Jörg Votteler, Liane Neumann contributed equally to this work.
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Votteler, J., Neumann, L., Hahn, S. et al. Highly conserved serine residue 40 in HIV-1 p6 regulates capsid processing and virus core assembly. Retrovirology 8, 11 (2011) doi:10.1186/1742-4690-8-11
- Equine Infectious Anemia Virus
- Virus Core
- Virus Release
- S40F Mutation
- Single Round Infection