Figure 8
From: Highly conserved serine residue 40 in HIV-1 p6 regulates capsid processing and virus core assembly
Analysis of Gag processing products and Env incorporation in wt HIV-1 and S40F mutant virions. (A) Analysis of Gag processing in VLPs. VLPs produced in 293T cells transiently transfected with pΔR and pΔRS40F were purified and analyzed by Western blot using antibodies against CA, MA and NC. (B) Analysis of Env incorporation in VLPs. VLPs produced in 293T cells transiently transfected with pΔR and pΔR S40F were purified and analyzed by Western blot using antibodies against Gag and Env.