Figure 7
From: Highly conserved serine residue 40 in HIV-1 p6 regulates capsid processing and virus core assembly
Mutation of Ser-40 has no effect on ALIX mediated virus release. (A) 293T cells were cotransfected with HIV-1ΔPTAP or HIV-1ΔPTAP/S40F and increasing amount of ALIX plasmid. (B) Quantification of Western blot data using AIDA Software (Raytest). Shown is the amount of Gag release determined by the ratio of virus associated Gag/total Gag expressed. (C) The amount of infectious units released from the cells was analyzed by β-galactosidase quantification after infection of TZM-bl cells.