Figure 10
From: Highly conserved serine residue 40 in HIV-1 p6 regulates capsid processing and virus core assembly
Rescue of CA maturation by A1V mutation in CA-SP1 cleavage site. (A) Western blot analysis of CA processing in VLPs. VLPs derived from 293T cells transfected with pΔR and pΔRS40F in combination with the A1V mutation in SP1 were purified and analyzed by Western blot using antibodies against CA. (B) Quantitative assessment of the viral core morphology derived from electron micrographs for the above indicated mutants. About 100-120 unselected particles of the wt virus and each mutant were evaluated. (C) Specific infectivity of HIV-1NL4-3 wt and S40F mutant in combination with the A1V mutation. TZM-bl cells were infected with virions derived from pNL4-3 wt and S40F in combination with SP1 A1V and infectious titers were determined by measurement of the β-galactosidase activity (RLU: relative light units).