Figure 3
From: Highly conserved serine residue 40 in HIV-1 p6 regulates capsid processing and virus core assembly
Replication of HIV-1 NL4-3 wt and S40F mutant in CEMs and HLAC. CEM cells were inoculated with purified virus equivalent to 20 ng (A) or 50 ng (B) of p24 bearing indicated mutations, and medium was collected every two days. For replication analyses in HLAC, X4 tropic (C and E) and R5 tropic (D and F), 1 ng of p24 of purified virus carrying indicated mutations was used for infection and release of viral particles was determined in the supernatant every third day (C and D: donor A, E and F: donor B).