Figure 2

Mutation of Ser-40 does neither affect the secondary structure of p6, nor the interaction with ALIX. A) Chemical shift differences (ppm) of the α-protons between the experimental values and those for residues in a random coil for sp6^23-52wt and sp6^23-52S40F in 50% aqueous TFE at pH 3 at 300 K. All positive values for N-terminal residues adjacent to proline residues at positions 24, 30, 37 and 49 arise from an inherent effect of proline and not out of a structural perturbation. This was explained in detail previously [25].