- Open Access
Diminished transmission of drug resistant HIV-1 variants with reduced replication capacity in a human transmission model
- Marieke Pingen†1, 2,
- Ramin Sarrami-Forooshani†3,
- Annemarie MJ Wensing1,
- Petra van Ham1,
- Agata Drewniak3,
- Charles AB Boucher2,
- Teunis BH Geijtenbeek3Email author and
- Monique Nijhuis1Email author
© Pingen et al.; licensee BioMed Central Ltd. 2014
- Received: 1 September 2014
- Accepted: 25 November 2014
- Published: 14 December 2014
Different patterns of drug resistance are observed in treated and therapy naïve HIV-1 infected populations. Especially the NRTI-related M184I/V variants, which are among the most frequently encountered mutations in treated patients, are underrepresented in the antiretroviral naïve population. M184I/V mutations are known to have a profound effect on viral replication and tend to revert over time in the new host. However it is debated whether a diminished transmission efficacy of HIV variants with a reduced replication capacity can also contribute to the observed discrepancy in genotypic patterns.
As dendritic cells (DCs) play a pivotal role in HIV-1 transmission, we used a model containing primary human Langerhans cells (LCs) and DCs to compare the transmission efficacy M184 variants (HIV-M184V/I/T) to HIV wild type (HIV-WT). As control, we used HIV harboring the NNRTI mutation K103N (HIV-K103N) which has a minor effect on replication and is found at a similar prevalence in treated and untreated individuals.
In comparison to HIV-WT, the HIV-M184 variants were less efficiently transmitted to CCR5+ Jurkat T cells by both LCs and DCs. The transmission rate of HIV-K103N was slightly reduced to HIV-WT in LCs and even higher than HIV-WT in DCs. Replication experiments in CCR5+ Jurkat T cells revealed no apparent differences in replication capacity between the mutant viruses and HIV-WT. However, viral replication in LCs and DCs was in concordance with the transmission results; replication by the HIV-M184 variants was lower than replication by HIV-WT, and the level of replication of HIV-K103N was intermediate for LCs and higher than HIV-WT for DCs.
Our data demonstrate that drug resistant M184-variants display a reduced replication capacity in LCs and DCs which directly impairs their transmission efficacy. As such, diminished transmission efficacy may contribute to the lower prevalence of drug resistant variants in therapy naive individuals.
- Drug resistance
- Dendritic cells
- Langerhans cells
HIV variants harboring drug-resistance mutations are detected in approximately 10% of all newly diagnosed patients in the Western world ,. Large clinical studies indicated that transmitted drug resistance may impact virological and immunological response to initial antiretroviral therapy ,. A change from a methionine to valine or isoleucine at position 184 (M184V/I) in reverse transcriptase (RT) is the most frequently observed resistance mutation in patients experiencing treatment failure - but is only rarely observed in untreated, newly diagnosed individuals using population-based sequencing assays ,,. M184V/I causes high level resistance against the nucleoside reverse transcriptase inhibitors lamivudine and emtricitabine, but at the same time considerably decreases the processivity of reverse transcriptase (RT) resulting in a reduced viral replication capacity (RC) ,. Contrary to M184V, the frequently observed RT mutation K103N has a similar prevalence in treated and untreated patients . The presence of K103N causes high levels of resistance against the non-nucleoside reverse transcriptase inhibitors efavirenz and nevirapine. K103N has been described to have a modest effect on viral RC ,, and can persist for years after transmission to a new host .
The low prevalence of the M184V mutation in therapy-naive individuals may be explained by the reduced RC of this mutant, which can directly impair transmission efficacy and/or lead to reversion of M184V in the new host. When HIV variants harboring M184V are transmitted to a new host, rapid reversion of the M184V variant has been documented (reviewed in ). Accordingly, the M184V variant can be detected as a minority species in recently infected individuals using very sensitive assays, which is suggestive of reversion to wild type . In addition, diminished transmission efficacy of the M184V variant has been suggested based on mathematical modeling and a macaque SHIV model ,. However, the impact of RC on transmission efficacy has never been investigated in a human transmission model.
Sexual HIV transmission is an inefficient process during which a limited number of virions initiate an infection in a new host, resulting in a severe transmission bottleneck ,. Although CD4+ T cells are the predominant target cells of HIV, it has been postulated that dendritic cells (DCs) naturally residing in the genital mucosa play a major role during sexual transmission -. Within the genital mucosa, Langerhans cells (LCs) reside in the epithelial layer and are the first DC subset encountered by HIV. LCs express the C-type lectin receptor langerin that captures HIV, leading to internalization and degradation of HIV. LCs therefore function as a natural barrier against HIV transmission. However, when the protective function of langerin is saturated, for example in the presence of a high inoculum or when langerin is downregulated due to cell maturation, LCs can become infected and subsequently transmit HIV to T cells . Furthermore, DC-SIGN+ DCs, which reside in the sub-epithelium, can transmit HIV to T cells. These DCs express the C-type lectin DC-SIGN that efficiently captures HIV and transmits the virus to T cells . Transmission of HIV by DCs and LCs may occur either as a result of infection of DCs/LCs and subsequent de novo virus replication (in cis), or by uptake and transfer of virions (in trans). Both mechanisms have been observed in in vitro studies -. The objective of this study was to investigate the transmission efficacy of the HIV-1 M184V/I RT variants. We used an HIV transmission model containing primary human DCs to compare the transmission efficacy of HIV harboring M184V/I to wild type HIV. With this virus panel, we demonstrated that the M184V/I variants were less efficiently transmitted to CCR5+ Jurkat T cells by both LCs and DCs, which was due to the lower RC of the M184V/I variants in both DC subsets. These results clearly imply a role for HIV RC in transmission efficacy and provide an additional explanation for the low prevalence of HIV M184V/I in therapy naïve individuals.
Impact of drug resistance mutations on transmission by LCs and DCs
We hypothesized that due to a diminished replication potential , HIV harboring M184V/I is less efficiently transmitted than HIV-WT or drug resistant virus variants with a high RC . Therefore, we compared the transmission efficacy of HIV-M184V/I to HIV-WT and HIV-K103N. To gain more insight in the impact of RC on transmission efficacy, we additionally investigated the drug resistant HIV-M184T variant. The RC of HIV-M184T is even more impaired than the RC of M184V/I, and as such this variant is rarely observed in vivo but can be selected in vitro ,. Since DCs play an important role during HIV transmission ,, we assessed the transmission efficacy of this virus panel by two subsets of DCs: primary human LCs and human monocyte-derived immature DCs. Migratory LCs were isolated from the epidermis of skin obtained after plastic surgery from multiple healthy donors (purity: >95%, described in ref ) and exposed to HIV for four days to enable infection. A low and a high inoculum (17.5 and 100 ng p24) were used in the infections to ensure that the observed results are due to differences in infection and replication rather than caused by differences in titre of the virus stock or saturation of our transmission model. Virus input was based on p24 protein levels as this excludes any impact of the RC of the virus on viral input.
Replication capacity of mutant viruses in target cells
As an additional control, the transmission experiments were repeated using different target cells and a single cycle read out. Therefore, LCs and DCs were exposed to the HIV variants as aforementioned, but replication in TZM-bl target cells was limited to one round of replication by (pre-)incubation with the protease inhibitor indinavir. In line with the results obtained with CCR5+ Jurkat T cells, HIV-M184 variants and HIV-K103N were less efficiently transmitted by LCs than HIV-WT. The transmission efficacy of LCs and DCs to TZM-bl target cells was also comparable to the CCR5+ Jurkat T cells; the HIV-M184 variants were less frequently transmitted as HIV-WT and the HIV-K103N transmission efficacy was comparable to HIV-WT (Figure 3B-C). These data suggest that the diminished transmission of M184 variants by LCs and DCs is not caused by differential RC in the target cells.
Replication capacity of mutant viruses in LCs and DCs
We have investigated the impact of drug resistance mutations in HIV RT on viral transmission efficacy. K103N and M184V are both frequently observed in patients experiencing therapy failure, but whereas M184V is rarely detected in newly diagnosed patients, K103N is often observed ,. It was debated whether a diminished transmission efficacy could contribute to this observed discrepancy in prevalence. We compared transmission of wild type HIV with HIV-M184V in an HIV transmission model containing primary human LCs or DCs. In addition, we investigated transmission of HIV-K103N and HIV-M184T as controls. We demonstrated that transmission by LCs and DCs to T cells is affected by the replication capacity defect caused by the M184 mutation.
Our results are in line with a study that compared transmission of SHIV wild type and M184V in rhesus macaques. In a repeated low-dose rectal transmission model, a larger inoculum was needed to successfully infect macaques with a SHIV variant containing M184V, indicating that mucosal transmissibility of the M184V variant is lower than wild type .
It has been known for a long time that the RC of HIV harboring M184V/I/T is reduced in primary T cells ,. Our data demonstrate that replication in primary LCs and DCs is also affected and as a result, transmission to T cells is diminished. We have previously demonstrated that the impact of M184VIT is more pronounced in primary cells containing low levels of dNTPs . In myeloid cells such as DCs, SAMHD-1 lowers the intracellular dNTP levels  and as such may impair the replication of the HIV-M184 variants .
The frequently observed NNRTI-related mutation K103N has been described as having a modest impact on RC by several ,,, but not all  previous studies. This discrepancy may be caused by differences in the assays that were used to determine viral RC in these studies, such as the viral genetic background or cell type. In our HIV transmission model, the infection of and transmission by primary DCs and LCs of HIV-K103N was consistently higher than the HIV-M184 variants. Remarkably, the level of infection of DCs by the K103N mutant was even higher than HIV-WT in the majority of donors.
Several studies have addressed transmission efficacy in humans by comparing the prevalence of drug-resistant HIV variants in newly diagnosed patients and treatment-experienced patients ,,. These studies observed a reduced transmission rate of HIV variants harboring drug resistance mutations. Such in vivo approaches measure the net result of potential differences in transmission efficacy combined with potential reversion of drug-resistance mutations after transmission to the new host. M184V is known to revert rapidly after transmission . Indeed, the Swiss HIV cohort study described a lower prevalence of HIV-M184V in acutely infected HIV individuals compared to chronically infected patients . Using in vitro experiments, we were able to exclusively investigate the impact of drug resistance mutations on the transmission efficacy. DCs can either be productively infected (cis-infection), or transfer captured virions by trans infection -. In our in vitro HIV transmission model, cis infection of LCs and DCs plays an important role ,. Using this in vitro model, we were able to demonstrated that HIV-M184 variants not only have a lower RC in primary T cells, but also in DCs and LCs which decreases the transmission efficacy of these drug resistant HIV variants.
Our data indicate that the RC of HIV variants with RT drug resistance mutations can impact the transmission efficacy. This may contribute to the discrepancy of the prevalence of M184V in treatment-experienced and naive individuals. In addition to RT drug resistance mutations, also variants harboring protease or integrase inhibitor resistance mutations decrease viral RC ,. Determination of the impact of mutations affecting other steps in the viral replication cycle on transmission efficacy may enhance our understanding of the role of RC in transmission. In addition, DCs are important targets for the transmission of several other viruses such as measles virus, herpes simplex virus and phleboviruses -. As such, one could hypothesize that differences in RC in DCs also impact transmission of other viruses.
We have shown a diminished transmission of M184 variants from LCs and DCs to target cells, which was likely caused by the lower RC of M184 variants in LCs and DCs. Therefore, a diminished transmission efficacy of drug-resistant variants provides an additional mechanism explaining the observed discrepancy in prevalence of replication-deficient drug-resistant HIV variants in treatment-experienced and naive individuals.
The site-directed mutants M184V, M184I and M184T were previously generated , in the background of HXB2. The mutation resulting in amino acid change K103N was introduced in HXB2 by site-directed mutagenesis using the previously described vector system with addition of primer K103N (5′-GTTACTGATTTGTTCTTTTTTAACCC-′3) . Tropism of all viral variants was changed from CXCR4-tropic to CCR5-tropic by replacing the HXB2-V3 loop with the V3 loop of the CCR5-tropic lab strain BaL). HXB2-cBaL, referred to as HIV-WT, was generated by introducing a unique BmgBI-restriction site at position 7091 in HXB2. After restriction with BmgBI and NheI, nucleotides 7091 to 7260 of HXB2 were replaced by V3 of BaL. Subsequently, the plasmids containing drug-resistance mutations were restricted by NcoI and NheI and nucleotides 5675 to 7260 of these plasmids were replaced by the corresponding region of HXB2-cBaL.
Virus was obtained by transfection of HEK293T cells with plasmid DNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol. To exclude a possible influence of different batches, all steps of virus production were performed synchronized. Virus was quantified by p24 analysis.
CCR5+ Jurkat T cells were generated and maintained as previously described . TZM-bl cells that express CCR5 were obtained through the NIH AIDS Research and Reference Reagent Program and maintained as recommended. Donor PBMCs were obtained by Ficoll-Paque density gradient centrifugation of heparinized blood from five HIV-seronegative donors, pooled and stored at −80°C until use. PBMCs were stimulated for three days with phytohaemagglutinin (2 mg/l) in culture medium (RPMI 1640 with L-glutamine (Lonza, Verviers, Belgium), 10% fetal bovine serum (Sigma-Aldrich, Zwijndrecht, the Netherlands) and 10 μg/ml gentamicin (Invitrogen, Breda, the Netherlands). LCs and DCs were obtained from multiple donors as previously described . In short, for LC isolation, epidermis was separated by dispase II treatment (1 mg/ml, Roche Diagnostic Systems, Somervolle, NJ) and cultured until LC maturation and migration. The resulting cell suspension was purified by CD1a positive selection using MACS, according to the manufacturer’s protocol (Miltenyi Biotec, Auburn, CA). This procedure yielded a >95% pure CD1a+ LC population (Figure 1A). The small population of contaminating cells mostly consists of keratinocytes. Monocytes were isolated by density centrifugation of PBMCs and cultured for five days in the presence of 800 U/ml IL-4 and 1000 U/ml GM-CSF to stimulate differentiation into DCs. The purity of obtained DCs was >90% (Figure 2A). Less than 5% of the contaminating cells are T cells which are not found to be productively infected, and excluded from analysis using gating on CD1+ cells in flow cytometry.
Viral replication capacity
RC in CCR5+ Jurkat T cells was determined in duplicate by infecting 2×106 cells with the equivalent of 40 ng p24 of each virus. After two hours of inoculation, cells were washed twice with RPMI 1640 medium with L-glutamine and resuspended in 10 ml culture medium with 5 U/ml IL-2 (Roche, Mannheim, Germany). 300 μl cell-free supernatant was harvested daily for p24 analysis. Cultures were maintained for 14 days.
Infection of DCs and LCs was determined by infecting 50,000 cells with 17.5 or 100 ng p24. After 6 days of infection, cells were stained with α-CD1a as a marker for DCs and α-p24 as marker for productive HIV infection. Living cells were gated based on forward and sideward scatter; DCs were distinguished from contaminating cells based on CD1a expression.
In replication competition experiments, DCs were infected with 100 ng p24 of both HIV-WT and HIV-M184T. After inoculation, cells were washed and the ratio of mutant and WT viral RNA was determined using viral population sequencing.
Intracellular mRNA was isolated using the mRNA capture kit according to manufacturer’s protocol (Roche). cDNA was synthesized using a RNA-to-cDNA kit (Promega). Quantitative PCR was performed to determine Tat-Rev, an early expressed viral mRNA, using a SYBR green approach using the following primers: 5′-ATGGCAGGAAGAAGCGGAG-3′ and 5′-ATTCCTTCGGGCCTGTCG-3′. Viral gene expression was normalized to housekeeping gene GAPDH as previously described .
50,000 DCs or LCs were infected with the equivalent of 17.5 and 100 ng p24 of all HIV variants for four to five days. After extensive washing, DCs or LCs were added to the target cells, which were either 50,000 CCR5+ Jurkat T cells or TZM-bl cells that were pre-seeded on 96 well plate (confluence 70%). After 1–4 days of co-culture, infection was measured by flow cytometry as described above (CCR5+ Jurkat T cells) or by luminescence (TZM-bl cells). TZM-bl cells were (pre-)incubated with 1,000 nM indinavir to investigate a single replication cycle after transmission.
The following antibodies were used: CD1a-FITC (BD Pharmingen, San Diego, CA, USA), CD1a-APC, CD3-PE (both BD Bioscience, San Jose, CA, USA), CD4-PerCP (BD Pharmingen), CD4-Alexa488 (Biolegend, San Diego, CA, USA), CCR5-APC (CD195) (BD Pharmingen), CXCR4-PerCP (R&D systems, Minneapolis, MN, USA), Langerin-PE (CD207), p24-PE (both Beckman Coulter, Fullerton, CA, USA).
We thank Susan C. Aitken for critical reading of the manuscript and Dorien de Jong for technical assistance.
This study was supported by the Aids Fonds Netherlands [grant number 2007034], the Netherlands Organization for Scientific Research [NWO VIDI grant 91796349 and VICI 91810619].
- Wheeler WH, Ziebell RA, Zabina H, Pieniazek D, Prejean J, Bodnar UR, Mahle KC, Heneine W, Johnson JA, Hall HI: Prevalence of transmitted drug resistance associated mutations and HIV-1 subtypes in new HIV-1 diagnoses, U.S.-2006. AIDS. 2010, 24: 1203-1212. 10.1097/QAD.0b013e3283388742.View ArticlePubMedGoogle Scholar
- Vercauteren J, Wensing AM, van de Vijver DA, Albert J, Balotta C, Hamouda O, Kucherer C, Struck D, Schmit JC, Asjo B, Bruckova M, Camacho RJ, Clotet B, Couglan S, Grossman Z, Horban A, Korn K, Kostrikis L, Nielsen C, Paraskevis D, Poljak M, Puchhammer-Stockl E, Riva C, Ruiz L, Salminen M, Schuurman R, Sonnerborg A, Stanekova D, Stanojevic M, Vandamme AM, Boucher CA: Transmission of drug-resistant HIV-1 is stabilizing in Europe. J Infect Dis. 2009, 200: 1503-1508. 10.1086/644505.View ArticlePubMedGoogle Scholar
- Wittkop L, Gunthard HF, de Wolf F, Dunn D, Cozzi-Lepri A, de Luca A, Kucherer C, Obel N, von Wyl V, Masquelier B, Stephan C, Torti C, Antinori A, Garcia F, Judd A, Porter K, Thiebaut R, Castro H, van Sighem AI, Colin C, Kjaer J, Lundgren JD, Paredes R, Pozniak A, Clotet B, Phillips A, Pillay D, Chene G: Effect of transmitted drug resistance on virological and immunological response to initial combination antiretroviral therapy for HIV (EuroCoord-CHAIN joint project): a European multicohort study. Lancet Infect Dis. 2011, 11: 363-371. 10.1016/S1473-3099(11)70032-9.View ArticlePubMedGoogle Scholar
- Kuritzkes DR, Lalama CM, Ribaudo HJ, Marcial M, Meyer WA, Shikuma C, Johnson VA, Fiscus SA, D'Aquila RT, Schackman BR, Acosta EP: Gulick RMl: Preexisting resistance to nonnucleoside reverse-transcriptase inhibitors predicts virologic failure of an efavirenz-based regimen in treatment-naive HIV-1-infected subjects. J Infect Dis. 2008, 197: 867-870. 10.1086/528802.View ArticlePubMedGoogle Scholar
- Wainberg MA, Moisi D, Oliveira M, Toni TD, Brenner BG: Transmission dynamics of the M184V drug resistance mutation in primary HIV infection. J Antimicrob Chemother. 2011, 66: 2346-2349. 10.1093/jac/dkr291.View ArticlePubMedGoogle Scholar
- Richman DD, Morton SC, Wrin T, Hellmann N, Berry S, Shapiro MF, Bozzette SA: The prevalence of antiretroviral drug resistance in the United States. AIDS. 2004, 18: 1393-1401. 10.1097/01.aids.0000131310.52526.c7.View ArticlePubMedGoogle Scholar
- van de Vijver DA, Wensing AM, Asjo B, Bruckova M, Jorgensen LB, Camacho R, Horban A, Linka M, Lazanas M, Loveday C, MacRae E, Nielsen C, Paraskevis D, Poljak M, Puchhammer-Stockl E, Ruiz L, Schmit JC, Stanczak G, Stanojevic M, Vandamme AM, Vercauteren J, Zazzi M, Bacheler L, Lecocq P, Villacian J, Boucher CA: HIV-1 drug-resistance patterns among patients on failing treatment in a large number of European countries. Acta Dermatovenerol Alp Panonica Adriat. 2010, 19: 3-9.Google Scholar
- Schuurman R, Nijhuis M, van Leeuwen R, Schipper P, de Jong D, Collis P, Danner SA, Mulder J, Loveday C, Christopherson C, Kwok S, Sninsky J, Boucher CA: Rapid changes in human immunodeficiency virus type 1 RNA load and appearance of drug-resistant virus populations in persons treated with lamivudine (3TC). J Infect Dis. 1995, 171: 1411-1419. 10.1093/infdis/171.6.1411.View ArticlePubMedGoogle Scholar
- Back NK, Nijhuis M, Keulen W, Boucher CA, Oude Essink BO, van Kuilenburg AB, van Gennip AH, Berkhout B: Reduced replication of 3TC-resistant HIV-1 variants in primary cells due to a processivity defect of the reverse transcriptase enzyme. EMBO J. 1996, 15: 4040-4049.PubMed CentralPubMedGoogle Scholar
- Armstrong KL, Lee TH, Essex M: Replicative fitness costs of nonnucleoside reverse transcriptase inhibitor drug resistance mutations on HIV subtype C. Antimicrob Agents Chemother. 2011, 55: 2146-2153. 10.1128/AAC.01505-10.PubMed CentralView ArticlePubMedGoogle Scholar
- Xu HT, Oliveira M, Quan Y, Bar-Magen T, Wainberg MA: Differential impact of the HIV-1 non-nucleoside reverse transcriptase inhibitor mutations K103N and M230L on viral replication and enzyme function. J Antimicrob Chemother. 2010, 65: 2291-2299. 10.1093/jac/dkq338.View ArticlePubMedGoogle Scholar
- Pingen M, Nijhuis M, de Bruijn JA, Boucher CA, Wensing AM: Evolutionary pathways of transmitted drug-resistant HIV-1. J Antimicrob Chemother. 2011, 66: 1467-1480. 10.1093/jac/dkr157.View ArticlePubMedGoogle Scholar
- Cong ME, Youngpairoj AS, Aung W, Sharma S, Mitchell J, Dobard C, Heneine W, Garcia-Lerma JG: Generation and mucosal transmissibility of emtricitabine- and tenofovir-resistant SHIV162P3 mutants in macaques. Virology. 2011, 412: 435-440. 10.1016/j.virol.2011.01.038.View ArticlePubMedGoogle Scholar
- Leigh Brown AJ, Frost SD, Mathews WC, Dawson K, Hellmann NS, Daar ES, Richman DD, Little SJ: Transmission fitness of drug-resistant human immunodeficiency virus and the prevalence of resistance in the antiretroviral-treated population. J Infect Dis. 2003, 187: 683-686. 10.1086/367989.View ArticlePubMedGoogle Scholar
- Miller CJ, Li Q, Abel K, Kim EY, Ma ZM, Wietgrefe S, La Franco-Scheuch L, Compton L, Duan L, Shore MD, Zupancic M, Busch M, Carlis J, Wolinsky S, Haase AT: Propagation and dissemination of infection after vaginal transmission of simian immunodeficiency virus. J Virol. 2005, 79: 9217-9227. 10.1128/JVI.79.14.9217-9227.2005.PubMed CentralView ArticlePubMedGoogle Scholar
- Keele BF, Giorgi EE, Salazar-Gonzalez JF, Decker JM, Pham KT, Salazar MG, Sun C, Grayson T, Wang S, Li H, Wei X, Jiang C, Kirchherr JL, Gao F, Anderson JA, Ping LH, Swanstrom R, Tomaras GD, Blattner WA, Goepfert PA, Kilby JM, Saag MS, Delwart EL, Busch MP, Cohen MS, Montefiori DC, Haynes BF, Gaschen B, Artheya GS, Lee HY, Wood N, Seoighe C, Perelson AS, Bhattacharya T, Korber BT, Hahn BH, Shaw GM: Identification and characterization of transmitted and early founder virus envelopes in primary HIV-1 infection. Proc Natl Acad Sci U S A. 2008, 105: 7552-7557. 10.1073/pnas.0802203105.PubMed CentralView ArticlePubMedGoogle Scholar
- Spira AI, Marx PA, Patterson BK, Mahoney J, Koup RA, Wolinsky SM, Ho DD: Cellular targets of infection and route of viral dissemination after an intravaginal inoculation of simian immunodeficiency virus into rhesus macaques. J Exp Med. 1996, 183: 215-225. 10.1084/jem.183.1.215.View ArticlePubMedGoogle Scholar
- Hladik F, Sakchalathorn P, Ballweber L, Lentz G, Fialkow M, Eschenbach D, McElrath MJ: Initial events in establishing vaginal entry and infection by human immunodeficiency virus type-1. Immunity. 2007, 26: 257-270. 10.1016/j.immuni.2007.01.007.PubMed CentralView ArticlePubMedGoogle Scholar
- Hu J, Gardner MB, Miller CJ: Simian immunodeficiency virus rapidly penetrates the cervicovaginal mucosa after intravaginal inoculation and infects intraepithelial dendritic cells. J Virol. 2000, 74: 6087-6095. 10.1128/JVI.74.13.6087-6095.2000.PubMed CentralView ArticlePubMedGoogle Scholar
- Fahrbach KM, Barry SM, Anderson MR, Hope TJ: Enhanced cellular responses and environmental sampling within inner foreskin explants: implications for the foreskin's role in HIV transmission. Mucosal Immunol. 2010, 3: 410-418. 10.1038/mi.2010.18.PubMed CentralView ArticlePubMedGoogle Scholar
- de Witte L, Nabatov A, Pion M, Fluitsma D, de Jong MA, de Gruijl T, Piguet V, van Kooyk Y, Geijtenbeek TB: Langerin is a natural barrier to HIV-1 transmission by Langerhans cells. Nat Med. 2007, 13: 367-371. 10.1038/nm1541.View ArticlePubMedGoogle Scholar
- Geijtenbeek TB, Kwon DS, Torensma R, van Vliet SJ, van Duijnhoven GC, Middel J, Cornelissen IL, Nottet HS, KewalRamani VN, Littman DR, Figdor CG, van Kooyk Y: DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells. Cell. 2000, 100: 587-597. 10.1016/S0092-8674(00)80694-7.View ArticlePubMedGoogle Scholar
- Turville SG, Santos JJ, Frank I, Cameron PU, Wilkinson J, Miranda-Saksena M, Dable J, Stossel H, Romani N, Piatak M, Lifson JD, Pope M, Cunningham AL: Immunodeficiency virus uptake, turnover, and 2-phase transfer in human dendritic cells. Blood. 2004, 103: 2170-2179. 10.1182/blood-2003-09-3129.View ArticlePubMedGoogle Scholar
- Peressin M, Proust A, Schmidt S, Su B, Lambotin M, Biedma ME, Laumond G, Decoville T, Holl V, Moog C: Efficient transfer of HIV-1 in trans and in cis from Langerhans dendritic cells and macrophages to autologous T lymphocytes. AIDS. 2014, 28: 667-677. 10.1097/QAD.0000000000000193.View ArticlePubMedGoogle Scholar
- Sarrami-Forooshani R, Mesman AW, van Teijlingen NH, Sprokholt JK, van der Vlist M, Ribeiro CM, Geijtenbeek TB: Human immature Langerhans cells restrict CXCR4-using HIV-1 transmission. Retrovirology. 2014, 11: 52-10.1186/1742-4690-11-52.PubMed CentralView ArticlePubMedGoogle Scholar
- Keulen W, Back NK, van Wijk A, Boucher CA, Berkhout B: Initial appearance of the 184Ile variant in lamivudine-treated patients is caused by the mutational bias of human immunodeficiency virus type 1 reverse transcriptase. J Virol. 1997, 71: 3346-3350.PubMed CentralPubMedGoogle Scholar
- Romani N, Gruner S, Brang D, Kampgen E, Lenz A, Trockenbacher B, Konwalinka G, Fritsch PO, Steinman RM, Schuler G: Proliferating dendritic cell progenitors in human blood. J Exp Med. 1994, 180: 83-93. 10.1084/jem.180.1.83.View ArticlePubMedGoogle Scholar
- Laguette N, Sobhian B, Casartelli N, Ringeard M, Chable-Bessia C, Segeral E, Yatim A, Emiliani S, Schwartz O, Benkirane M: SAMHD1 is the dendritic- and myeloid-cell-specific HIV-1 restriction factor counteracted by Vpx. Nature. 2011, 474: 654-657. 10.1038/nature10117.PubMed CentralView ArticlePubMedGoogle Scholar
- Collins JA, Thompson MG, Paintsil E, Ricketts M, Gedzior J, Alexander L: Competitive fitness of nevirapine-resistant human immunodeficiency virus type 1 mutants. J Virol. 2004, 78: 603-611. 10.1128/JVI.78.2.603-611.2004.PubMed CentralView ArticlePubMedGoogle Scholar
- Cong ME, Heneine W, Garcia-Lerma JG: The fitness cost of mutations associated with human immunodeficiency virus type 1 drug resistance is modulated by mutational interactions. J Virol. 2007, 81: 3037-3041. 10.1128/JVI.02712-06.PubMed CentralView ArticlePubMedGoogle Scholar
- Yerly S, Jost S, Telenti A, Flepp M, Kaiser L, Chave JP, Vernazza P, Battegay M, Furrer H, Chanzy B, Burgisser P, Rickenbach M, Gebhardt M, Bernard MC, Perneger T, Hirschel B, Perrin L: Infrequent transmission of HIV-1 drug-resistant variants. Antivir Ther. 2004, 9: 375-384.PubMedGoogle Scholar
- de Mendoza C, Rodriguez C, Corral A, del Romero J, Gallego O, Soriano V: Evidence for differences in the sexual transmission efficiency of HIV strains with distinct drug resistance genotypes. Clin Infect Dis. 2004, 39: 1231-1238. 10.1086/424668.View ArticlePubMedGoogle Scholar
- Metzner KJ, Scherrer AU, Preiswerk B, Joos B, von Wyl V, Leemann C, Rieder P, Braun D, Grube C, Kuster H, Boni J, Yerly S, Klimkait T, Aubert V, Furrer H, Battegay M, Vernazza PL, Cavassini M, Calmy A, Bernasconi E, Weber R, Gunthard HF: Origin of minority drug-resistant HIV-1 variants in primary HIV-1 infection. J Infect Dis. 2013, 208: 1102-1112. 10.1093/infdis/jit310.View ArticlePubMedGoogle Scholar
- Kawamura T, Gulden FO, Sugaya M, McNamara DT, Borris DL, Lederman MM, Orenstein JM, Zimmerman PA, Blauvelt A: R5 HIV productively infects Langerhans cells, and infection levels are regulated by compound CCR5 polymorphisms. Proc Natl Acad Sci U S A. 2003, 100: 8401-8406. 10.1073/pnas.1432450100.PubMed CentralView ArticlePubMedGoogle Scholar
- Cavrois M, Neidleman J, Kreisberg JF, Greene WC: In vitro derived dendritic cells trans-infect CD4 T cells primarily with surface-bound HIV-1 virions. PLoS Pathog. 2007, 3: e4-10.1371/journal.ppat.0030004.PubMed CentralView ArticlePubMedGoogle Scholar
- Ballweber L, Robinson B, Kreger A, Fialkow M, Lentz G, McElrath MJ, Hladik F: Vaginal langerhans cells nonproductively transporting HIV-1 mediate infection of T cells. J Virol. 2011, 85: 13443-13447. 10.1128/JVI.05615-11.PubMed CentralView ArticlePubMedGoogle Scholar
- de Jong MA, de Witte L, Oudhoff MJ, Gringhuis SI, Gallay P, Geijtenbeek TB: TNF-alpha and TLR agonists increase susceptibility to HIV-1 transmission by human Langerhans cells ex vivo. J Clin Invest. 2008, 118: 3440-3452. 10.1172/JCI34721.PubMed CentralView ArticlePubMedGoogle Scholar
- Martinez-Picado J, Savara AV, Sutton L, D'Aquila RT: Replicative fitness of protease inhibitor-resistant mutants of human immunodeficiency virus type 1. J Virol. 1999, 73: 3744-3752.PubMed CentralPubMedGoogle Scholar
- Hu Z, Kuritzkes DR: Effect of raltegravir resistance mutations in HIV-1 integrase on viral fitness. J Acquir Immune Defic Syndr. 2010, 55: 148-155. 10.1097/QAI.0b013e3181e9a87a.PubMed CentralView ArticlePubMedGoogle Scholar
- Lozach PY, Kuhbacher A, Meier R, Mancini R, Bitto D, Bouloy M, Helenius A: DC-SIGN as a receptor for phleboviruses. Cell Host Microbe. 2011, 10: 75-88. 10.1016/j.chom.2011.06.007.View ArticlePubMedGoogle Scholar
- de Witte L, de Vries RD, van der Vlist M, Yuksel S, Litjens M, de Swart RL, Geijtenbeek TB: DC-SIGN and CD150 have distinct roles in transmission of measles virus from dendritic cells to T-lymphocytes. PLoS Pathog. 2008, 4: e1000049-10.1371/journal.ppat.1000049.PubMed CentralView ArticlePubMedGoogle Scholar
- de Jong MA, de Witte L, Bolmstedt A, van Kooyk Y, Geijtenbeek TB: Dendritic cells mediate herpes simplex virus infection and transmission through the C-type lectin DC-SIGN. J Gen Virol. 2008, 89: 2398-2409. 10.1099/vir.0.2008/003129-0.View ArticlePubMedGoogle Scholar
- van Maarseveen NM, Huigen MC, de Jong D, Smits AM, Boucher CA, Nijhuis M: A novel real-time PCR assay to determine relative replication capacity for HIV-1 protease variants and/or reverse transcriptase variants. J Virol Methods. 2006, 133: 185-194. 10.1016/j.jviromet.2005.11.008.View ArticlePubMedGoogle Scholar
- Gringhuis SI, van der Vlist M, van den Berg LM, den Dunnen J, Litjens M, Geijtenbeek TB: HIV-1 exploits innate signaling by TLR8 and DC-SIGN for productive infection of dendritic cells. Nat Immunol. 2010, 11: 419-426. 10.1038/ni.1858.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.