Figure 1

Diminished transmission of M184 variants by LCs. LCs were exposed to the equivalent of 17.5 (open bars) or 100 ng (closed bars) p24 for up to four days, extensively washed and co-cultured for four days with CCR5⁺ Jurkat T cells. Cells were stained with antibodies against CD1a as a marker for LCs and intracellular p24 for HIV infection and analyzed by flow cytometry. A: The percentage of infected target CCR5⁺ Jurkat T cells (CD1a negative cells) at 2 days post transmission (dpt) (6 days post infection; dpi) (n = 3, representative donor depicted). B: The percentage of infected target cells (CD1a negative cells) at 1, 2 and 4 days post transmission (1 donor, only 100 ng p24 infection is shown). Abbreviation: n.c.: no infection control.