Figure 4

Transmission to target cells is driven by cis infection of DCs. DCs were infected in the absence and presence (diagonal striping marks) of AZT, a potent inhibitor of RT. All infections were started with virus equivalent to 17.5 ng (open bars) and 100 ng (filled bars) p24. Four days post infection, cells were extensively washed and co-cultured for two days with CCR5⁺ Jurkat T cells. Cells were stained with antibodies against CD1a as marker of DCs and intracellular p24 for HIV infection and analyzed by flow cytometry. The percentage p24 positive target cells is depicted. Data are representative for 2 donors.