Figure 2From: Diminished transmission of drug resistant HIV-1 variants with reduced replication capacity in a human transmission modelDiminished transmission of M184 variants by DCs. DCs were exposed to the equivalent of 17.5 (open bars) or 100 ng (closed bars) p24 for four days, extensively washed and co-cultured for two days with CCR5+ Jurkat T cells. Cells were stained with antibodies against CD1a as marker for DCs and intracellular p24 for HIV infection and analyzed by flow cytometry. A: The percentage of infected target cells (CD1a negative cells) at 2 days post transmission (dpt) (6 days post infection; dpi) (n = 4, representative for 3/4 donors). B: The percentage of infected target cells (CD1a negative cells) at 1, 2 and 4 days post transmission (n = 2, 100 ng p24 infection is shown). Abbreviation: n.c.: no infection control.Back to article page