Diminished transmission of M184 variants by DCs. DCs were exposed to the equivalent of 17.5 (open bars) or 100 ng (closed bars) p24 for four days, extensively washed and co-cultured for two days with CCR5+ Jurkat T cells. Cells were stained with antibodies against CD1a as marker for DCs and intracellular p24 for HIV infection and analyzed by flow cytometry. A: The percentage of infected target cells (CD1a negative cells) at 2 days post transmission (dpt) (6 days post infection; dpi) (n = 4, representative for 3/4 donors). B: The percentage of infected target cells (CD1a negative cells) at 1, 2 and 4 days post transmission (n = 2, 100 ng p24 infection is shown). Abbreviation: n.c.: no infection control.