The diminished transmission of M184 variants is not caused by replication in target cells. A: To determine the replication capacity of the virus panel in target cells, CCR5+ Jurkat T cells were infected in the absence of drugs and p24 production was monitored daily. Average infection with standard deviation is depicted. B-C: LCs (B) or DCs (C) were exposed to the equivalent of 17.5 (open bars) or 100 ng (closed bars) p24 for four days, extensively washed and co-cultured for two days with TZM-bl cells pre-incubated with indinavir. Infection was measured by luminescence compared to HIV-WT. Data are representative for the average with SD for 1 (LCs) and 2 (DCs) donors in duplo. Abbreviations: WT: HIV-WT, M184V: HIV-M184V, M184I: HIV-M184I, M184T: HIV-M184T, K103N: HIV-K103N, n.c.: no infection control.