Gammaretroviral pol sequences act in cis to direct polysome loading and NXF1/NXT-dependent protein production by gag-encoded RNA
© Bartels and Luban; licensee BioMed Central Ltd. 2014
Received: 17 April 2014
Accepted: 11 August 2014
Published: 12 September 2014
All retroviruses synthesize essential proteins via alternatively spliced mRNAs. Retrovirus genera, though, exploit different mechanisms to coordinate the synthesis of proteins from alternatively spliced mRNAs. The best studied of these retroviral, post-transcriptional effectors are the trans-acting Rev protein of lentiviruses and the cis-acting constitutive transport element (CTE) of the betaretrovirus Mason-Pfizer monkey virus (MPMV). How members of the gammaretrovirus genus translate protein from unspliced RNA has not been elucidated.
The mechanism by which two gammaretroviruses, XMRV and MLV, synthesize the Gag polyprotein (Pr65Gag) from full-length, unspliced mRNA was investigated here. The yield of Pr65Gag from a gag-only expression plasmid was found to be at least 30-fold less than that from an otherwise isogenic gag-pol expression plasmid. A frameshift mutation disrupting the pol open reading frame within the gag-pol expression plasmid did not decrease Pr65Gag production and 398 silent nucleotide changes engineered into gag rendered Pr65Gag synthesis pol-independent. These results are consistent with pol-encoded RNA acting in cis to promote Pr65Gag translation. Two independently-acting pol fragments were identified by screening 17 pol deletion mutations. To determine the mechanism by which pol promoted Pr65Gag synthesis, gag RNA in total and cytoplasmic fractions was quantitated by northern blot and by RT-PCR. The pol sequences caused, maximally, three-fold increase in total or cytoplasmic gag mRNA. Instead, pol sequences increased gag mRNA association with polyribosomes ~100-fold, a magnitude sufficient to explain the increase in Pr65Gag translation efficiency. The MPMV CTE, an NXF1-binding element, substituted for pol in promoting Pr65Gag synthesis. A pol RNA stem-loop resembling the CTE promoted Pr65Gag synthesis. Over-expression of NXF1 and NXT, host factors that bind to the MPMV CTE, synergized with pol to promote gammaretroviral gag RNA loading onto polysomes and to increase Pr65Gag synthesis. Conversely, Gag polyprotein synthesis was decreased by NXF1 knockdown. Finally, overexpression of SRp20, a shuttling protein that binds to NXF1 and promotes NXF1 binding to RNA, also increased gag RNA loading onto polysomes and increased Pr65Gag synthesis.
These experiments demonstrate that gammaretroviral pol sequences act in cis to recruit NXF1 and SRp20 to promote polysome loading of gag RNA and, thereby license the synthesis of Pr65Gag from unspliced mRNA.
Retroviruses compress large quantities of genetic information into their relatively small genomes. HIV-1, for example, has a single promoter that drives a primary transcript, from which 9 genes direct the synthesis of at least 15 proteins ,. This is accomplished by exploiting several mechanisms, including the synthesis of essential viral proteins from unspliced or partially spliced mRNAs ,,. In all retroviruses, the primary, unspliced transcript serves as the viral genomic RNA that is packaged into assembling virions. Unspliced RNA of identical primary sequence also directs translation of the main virion structural elements, the gag-encoded proteins . Assessment of HIV-1 transcripts by conventional methods has revealed nearly 50 variants , while newer deep sequencing technology has detected more than 100 . Replication of HIV-1 and other retroviruses must therefore necessitate an exquisite balance of these differentially spliced mRNAs .
Unspliced and or incompletely spliced RNAs are generally retained in the nucleus until splicing is completed, though the spliceosome is increasingly appreciated to function as a highly dynamic machine . Therefore, in order to generate viral proteins essential for virus replication, retroviruses must encode cis-acting RNA sequences that recruit trans-acting cellular factors, and, in some cases, trans-acting viral factors. Different classes of retroviruses have evolved unique mechanisms for exporting unspliced mRNAs out of the nucleus in such a way that they are efficiently translated. The lentivirus HIV-1, for example, encodes a trans-acting protein, Rev, that binds to a cis-acting RNA sequence in the unspliced mRNA called the Rev-Response Element (RRE); Rev then links the RNA to the CRM1-mediated export pathway ,-. In analogous fashion, the cis-acting constitutive transport element (CTE) in the betaretrovirus Mason-Pfizer monkey virus (MPMV) mRNA recruits the cellular export factor NXF1 -. Other betaretroviruses, including Jaagsiekte sheep retroviruse (JSRV), mouse mammary tumor virus (MMTV), and human endogenous retrovirus type K (ERV-K), additionally encode a trans-acting factor necessary for Gag protein production ,. The alpha-retrovirus Rous sarcoma virus (RSV) possesses two direct repeat (DR) sequences that function as CTE-like elements, though one copy is sufficient to provide translocation into the cytoplasm -. Avian leukosis virus (ALV) possesses a single DR in its 3′UTR region ,.
How unspliced gammaretroviral mRNAs are stabilized, exported from the nucleus, and translated into protein is not known. In the course of developing expression vectors for gammaretroviruses, we observed that gag coding sequences in isolation were unable to direct the synthesis of gag-encoded protein. This observation prompted investigation of post-transcriptional regulation in gammaretroviruses.
Gammaretrovirus pol increases the levels of the Gag polyprotein
In parallel with these experiments, the gag open reading frame from XMRV or MLV was cloned into identical expression plasmids in the absence of any pol sequences. When either the XMRV gag expression plasmid (Figure 1A, left panel) or the MLV gag expression plasmid (Figure 1A, right panel) were transfected into 293 T cells, to our surprise, Gag protein production was difficult to detect by western. Gag polyprotein production, then, was inefficient in the absence of pol.
To quantify the difference in the level of the XMRV Gag polyprotein, lysate from cells transfected with the gag-only expression plasmid was compared with serial dilutions of lysate from cells transfected with gag-pol. 48 hrs after 293 T cell transfection the lysates were probed in an immunoblot with a CA-specific antibody. The Gag polyprotein signal was at least 30-fold greater with gag-pol than it was with gag-only (Figure 1B).
The gammaretrovirus pol gene acts at the level of RNA to promote synthesis of the Gag polyprotein
The gag-pol expression plasmid containing the frameshift mutation was transfected into 293 T cells, in parallel with the wild-type gag-pol and gag-only expression plasmids. 48 hrs later, cell lysate was analyzed by immunoblot using anti-CA antibody (upper panel) or anti-β-actin antibody (lowel panel) (Figure 2B). Gag protein production by the gag-pol frameshift plasmid was indistinguishable from that of the wild-type gag-pol plasmid. This indicates that the pol sequence acts at the level of the RNA, and that it need not be translated into protein, to stimulate Gag polyprotein production.
The fact that pol RNA acted in cis to promote Gag polyprotein production suggested that the basis for the deficiency in Gag polyprotein production by the gag-alone plasmid would be at the level of gag RNA. To test if this was the case, a codon-optimized gag open reading-frame was generated and cloned into an isogenic expression plasmid and tested for its ability to generate Gag polyprotein in the absence of pol. The codon optimized gag has 398 silent nucleotide changes with respect to the original gag sequence and changes the GC content from 55% to 64%. When transfected in parallel with the other plasmids, the codon-optimized gag sequence increased Gag protein production well beyond the levels produced by the gag-pol plasmid (Figure 2B and C). This indicates that the relatively low levels of Gag polyprotein result from a deficiency at the level of gag RNA and that it does not result, for example, from protein instability.
The relatively low steady-state level of Gag polyprotein in the lysate of cells transfected with the gag-only plasmid might also be a consequence of Gag polyprotein budding off from the producer cell at a rate that exceeds the ability of the cell-associated protein to accumulate to detectable levels. To determine if this was the case, the supernatant was collected 48 hrs after transfection of the 293 T cells with the gag only and gag-pol expression plasmids. The supernatant was accelerated through a 25% sucrose cushion and protein in the pellet was immunoblotted with a CA-specific antibody (Figure 2C). The relative intensity of the Gag signal in the pellet tracked with the intensity of the signal in the cell lysate. This result indicates that the lower Gag polyprotein levels in the lysate of cells transfected with the gag-only plasmid did not result from an accelerated rate of virion budding and release.
Mapping of pol sequence required to promote Gag polyprotein production
The previous experiments demonstrated that pol sequences act at the level of the RNA to promote Gag polyprotein production. To map the pol sequences responsible for this activity an expression plasmid was engineered that contains XMRV gag followed by pol sequences, in such a way that translation of the pol sequences was precluded. Normally gammaretrovirus pol is expressed by read-through suppression of the gag UAG stop codon . The gag UAG stop codon in this construct was replaced with a UGA stop codon and the pol sequences were cloned such that they were out-of-frame with respect to gag. Either modification alone is sufficient to prevent pol translation.
To varying degrees, each of the nine engineered pol fragments that were tested increased Gag protein production above the level observed with gag alone (Figure 3B). Of the fragments tested, the 1611-2232 fragment had the smallest effect, only increasing Gag protein levels about 2-fold. Either of two pol fragments, 2232-3456 or 4543-5199, caused a large increase in Gag protein production. When combined together, the effect of 2232-3456 and 4543-5199 was additive, increasing Gag protein to the same level as the complete pol. These mapping results indicate that there are two regions within pol that are each independently capable of promoting Gag protein production, and that the two regions act together for the full effect (Figure 3B).
Mapping pol fragment 2232-3456
Mapping pol fragment 4543-5199
XMRV pol only modestly increases total and cytoplasmic gag mRNA levels
Quantitation of the bands on the northern blot using a phosphorimager revealed that, in the total RNA fraction, the signal was 2.33 to 2.65-fold higher for gag-pol than for gag, with a standard deviation of 0.18, when data for the three independent experiments was assessed. The cytoplasmic signal for gag-pol was 1.22 to 3.12-fold higher than for gag-only, with a standard deviation of 1.05 when data was combined for the three independent experiments (Figure 6C). The pol fragment 2232-3456 increased total RNA 2-fold and cytoplasmic gag mRNA levels three-fold compared to gag-only. pol fragment 4543-5199 increased total and cytoplasmic gag mRNA levels about two-fold compared to gag-only. These results demonstrate that pol has a small effect on the stability and the nuclear export to the cytoplasm of gag RNA. The magnitude of these effects is at least an order of magnitude too small to explain the much larger magnitude effect of pol on Gag protein production (Figure 1B). This suggests that there is an effect of pol RNA that occurs after nuclear export of the gag RNA.
pol promotes the association of gag mRNA with polyribosomes
Translation of gammaretroviral gag RNA is stimulated by fusion to the MPMV CTE or by overexpression of NXF1/TAP and NXT/p15
The MPMV CTE promotes translation of intron-containing RNAs by directly binding and recruiting NXF1/TAP, along with its cofactor NXT/p15 -. Previous studies showed that overexpression of NXF1 and NXT1 in 293 cells greatly boosted translation of HIV-1 gag-pol, but only when HIV-1 gag-pol was fused to the MPMV CTE . The effect of NXF1/NXT overexpression on gammaretroviral Gag protein production was examined next. Plasmids encoding FLAG-tagged NXF1 and FLAG-tagged NXT were co-transfected with the plasmid expressing gammaretroviral gag-only or with the plasmid expressing gag fused to pol fragment 2232-3456. A slight increase in Gag protein production was observed with the gag only plasmid (Figure 8B). A greater increase in Gag protein production was observed when gag was fused to pol fragment 2232-3456. Taken together, these results suggest that gammaretroviral pol recruits NXF1/NXT to promote Gag protein production in in manner similar to that of the MPMV CTE.
Gammaretroviral pol contains a CTE-like element that increases Gag protein production in an NXF1-dependent manner
Two experiments provided further evidence that the recruitment of NXF1 by the γ-CTE is relevant for stimulation of Gag polyprotein synthesis by pol. When the AAGACA motif in the γ-CTE was mutated to ATCGCG (γ-CTEmut), a mutation that was previously shown to disrupt NXF1 binding , the effect of the γ-CTE on Gag polyprotein production was attenuated (Figure 9B). Additionally, overexpression of NXF1 and NXT increased the effect of the γ-CTE on Gag polyprotein production (Figure 9C). Taken together, these results suggest that, at least in part, gammaretroviral pol increases Gag polyprotein synthesis by recruiting NXF1.
The impact of NXF1 knockdown on Gag protein production
Since NXF1 overexpression increased Gag polyprotein production, the effect of NXF1 knockdown was examined next. Three NXF1 target sequences were engineered into the miR30 framework of a previously described lentiviral knockdown vector that, additionally, confers resistance to puromycin ,. HEK293 cells transduced with either of the three NXF1 knockdown vectors were eliminated from the culture upon addition of puromycin. The magnitude of this apparent toxicity correlated with the efficiency of the knockdown, as assessed by western blot for NXF1. Cells transduced with a control vector targeting luciferase propagated normally in the presence of puromycin.
As compared with Gag polyprotein levels in the control luciferase knockdown cells, Gag polyprotein levels in the NXF1 knockdown cells were reduced, whether the Gag was encoded by the gag-pol plasmid or by the plasmid in which gag was fused to the γ-CTE (Figure 10B). Gag polyprotein levels produced by the gag alone plasmid were too low to test for reduction by NXF1 knockdown. When NXF1 levels were restored to supra-normal levels in the NXF1 knockdown cells by introduction of a non-targetable NXF1 expression plasmid, Gag polyprotein production was increased compared to the control cells (Figure 10B). NXF1 knockdown had no effect on the CRM1-dependent HIV-1 structural protein (Figure 10B), indicating that the reduction of gammaretroviral Gag polyprotein production was specific and not due to non-specific toxicity. These results indicate that gammaretroviral pol sequences promote Gag polyprotein production in an NXF1-dependent fashion.
SRp20 promotes gammaretrovirus Gag production by recruiting NXF1/NXT1
SRp20 has two main structural domains, an RNA recognition motif (RRM) and a carboxy-terminal RS domain. Based on previous work , two SRp20 mutants were generated. The first mutant, SRp20ΔRRM, lacks the RRM. This mutant retains the ability to bind NXF1 but is unable to bind RNA. When overexpressed, SRp20ΔRRM caused no detectable increase in Gag protein production (Figure 11C). The second mutant, SRp20R3A, has three arginines at amino acid positions 256, 262, and 268 in the RS domain all mutated to alanine. SRp20R3A retains the ability to bind RNA but is unable to recruit NXF1 . When SRp20R3A was co-transfected with the gammaretroviral gag expression plasmid, no significant increase in Gag protein production was observed (Figure 11C). Thus, both RNA binding via the RRM, and recruitment of NXF1 via the RS domain, are required for SRp20 stimulation of Gag protein production.
SRp20 increases gammaretroviral gag mRNA association with polysomes in an NXF1-dependent manner
The genomic RNA of all retroviruses, as well as the mRNA that is translated to produce the gag and pol gene products, resembles unspliced pre-mRNA in that it possesses splice donor and acceptor sites. Different classes of retroviruses have evolved unique mechanisms for exporting these unspliced mRNAs out of the nucleus in a manner that ensures efficient loading onto polysomes for translation. How unspliced gammaretrovirus mRNA is exported and translated has not been extensively investigated. Among retroviruses, gammaretroviruses are relatively simple in structure, possessing only three genes, gag, pol, and env, and lacking accessory proteins such as HIV-1 Rev that act in trans to promote the nuclear export of unspliced mRNA. It therefore seemed likely that gammaretroviruses would behave similar to the betaretrovirus MPMV that uses cis-acting elements in the viral RNA to recruit cellular factors that promote nuclear export and translation of the mRNA.
The studies here focused on Gag polyprotein translation directed by the unspliced gag mRNA from either of two gammaretroviruses, XMRV or Moloney-MLV. In isolation, the gag open reading frame was incapable of directing Gag polyprotein translation, even when gag transcription was driven by the potent CMV immediate early promoter (Figure 1). The primary sequence of the gag open reading frame was responsible for the block to translation since introduction of a large number of silent mutations permitted production of copious amounts of Gag polyprotein (Figure 2).
It was then demonstrated here that translation of gammaretroviral gag mRNA relies upon cis-acting sequences within pol (Figure 2). The location of these gammaretroviral elements is an interesting contrast to the well-characterized betaretroviral CTE which is located at the 3’ end of the MPMV genome. Analysis of gammaretroviral pol deletion mutations (Figure 3B) showed that two different fragments, one from nucleotides 2232-3456 (Figure 4B) and the other from nucleotides 4543-5199 (Figure 5B), contribute to gag mRNA translation. Though each fragment alone was quite potent at increasing Gag polyprotein levels, neither was equivalent to the complete pol sequence, indicating that the effect of the two fragments is additive. Our pol mapping results are in agreement with a recent report that also identified the 3′ end of pol as important for gammaretroviral Gag translation .
As determined by northern blot and by RT-PCR, each of the two pol sequences increased the steady-state level of total gag mRNA, as well as the level of cytoplasmic gag mRNA (Figure 6). But the magnitude of these effects was only a few fold, much smaller than the greater than 30-fold increase in Gag polyprotein translation associated with pol (Figure 1B). Rather, the main effect of the pol sequences was to promote loading of the gag mRNA onto polysomes, as determined by RT-PCR of the fractions obtained from a polysome gradient (Figure 7). In contrast to the results reported here, another group reported that the major effect of gammaretroviral pol is to promote nuclear export of either spliced or unspliced transcripts .
The biochemical characterization of the murine gammaretroviral elements described here, and elsewhere , was performed using human cells, and it is possible that their relative importance for RNA stability, transport, or polysome loading would be somewhat different in murine cells. That being said, though XMRV is a recombinant of two mouse retroviruses this virus was selected for replication in a human prostrate cancer cell line  and so the use of a human cell line for the XMRV biochemical studies described here is not unreasonable.
Clues to the mechanism of action of the gammaretroviral pol sequences were also obtained by considering the MPMV CTE, which was able to substitute for the pol sequences to promote gammaretroviral Gag polyprotein production (Figure 8A). It had been shown previously that a single copy of the MPMV CTE permits production of HIV-1 structural proteins in the absence of Rev , though in some contexts this required four tandem CTE repeats, or overexpression of the shuttling SR protein 9G8 or the export receptor NXF1/NXT ,,,. A single MPMV CTE was sufficient to promote gammaretroviral Gag production (Figure 8).
Given the similarity with MPMV, an RNA stem-loop with properties resembling the MPMV CTE was sought within gammaretroviral pol. A 100 nucleotide pol fragment was identified (Figure 9A) that bears a AAGACA motif like that previously shown to bind to NXF1 . Repeats of these stem-loops increased Pr65Gag production in a manner that was dependent on the AAGACA motif and that synergized with overexpression of NXF1/NXT (Figure 9B and C). Conversely, NXF1 knockdown decreased gammaretroviral Gag polyprotein production (Figure 10). Though NXF1 knockdown was toxic to the cells, and clearly limited the magnitude of the effect that was possible to detect, there was no effect on Rev-dependent synthesis of HIV-1 structural protein (Figure 10). These results suggest that gammaretroviral pol sequences recruit NXF1 to promote Gag polyprotein synthesis. Though direct binding between NXF1 and pol sequences was not demonstrated here, such an interaction was recently reported .
Gammaretroviral gag behaves like an intronless gene. 5% of eukaryotic genes naturally lack introns . The intronless histone H2A mRNA encodes a cis-acting element that recruits SR proteins ,, and subsequently NXF1/NXT, to promote nuclear export and translation -. SRp20 in particular recruits NXF1/NXT with which it forms a ternary complex . SR proteins are involved in post-transcriptional processing of mRNA ,, and contribute to the coupling of splicing, nuclear export and translation initiation ,. In some cases, NXF1/NXT associates with the NPC and promotes nuclear export of mRNAs by binding to adaptor proteins rather than by direct RNA binding ,.
Overexpression of NXF1/NXT increases the nucleocytoplasmic export of MPVM CTE-containing mRNAs by about 2-fold  and overexpression of mRNAs containing a CTE can block the nucleocytoplasmic export of cellular mRNAs ,. Sites on the RNA for direct NXF1-binding therefore seem to be saturable and therefore limiting (Figure 13). Overexpression of SRp20 appears to recruit more NXF1 to mRNA than NXF1 overexpression can provide, perhaps because the NXF1 binding sites on mRNA are less abundant than potential SRp20 binding sites (Figure 13). Gammaretroviral gag mRNA itself may possess SRp20 binding sites since SRp20 overexpression stimulated Gag polyprotein production by gag in the absence of pol sequences (Figures 11 and 12). According to this model, the increased SRp20 protein that is bound to gag mRNA then would recruit more NXF1, increasing the polysome loading of gag mRNA (Figures 12 and 13).
It has been shown that shuttling proteins like ASF/SF2 and 9G8 associate with polyribosomes ,,. The non-shuttling SR proteins like SRp40 only accompany the mRNA until the NPC and dissociate with their release into the cytoplasm. Consequently, SRp40 might not support the association of mRNA with polyribosomes and subsequent translation initiation. In fact, the non-shuttling SR protein, SRp40, did not promote Gag polyprotein translation like SRp20 (Figure 11B). Coyle and coworkers showed that the CTE element of MPMV mediates efficient export of reporter mRNA in HEK293T cells . In contrast to the experiments here, the overexpression of shuttling SR proteins had no effect on the nucleocytoplasmic export of CTE-containing mRNAs .
Other host factors were sought here that might contribute to gammaretroviral protein production. One of the best characterized mRNAs in mammalian cells is the β-actin mRNA. Proper localization of β-actin mRNA within the cell mediates cell migration during embryogenesis, differentiation and possibly carcinogenesis -. The zip code binding protein 1 (Zbp1) prevents premature translation of the β-actin mRNA during transport. Once the β-actin mRNA reaches its final destination Zbp1 is released from the mRNA and translation can occur . To determine if translational repression by the human homologue of Zbp1 (known as IMP1 or IGF2BP1) inhibits Gag polyprotein translation Imp1 was efficiently knocked down in HEK293T cells. No effect of IMP1 knockdown on Gag polyprotein production was observed (data not shown).
Tpr is a 254 kDa protein localized to the nuclear basket of the nuclear pore complex ,. Tpr suppresses expression of unspliced and intron-containing mRNA by inhibiting the export of CTE-containing RNA but not Rev-dependent mRNA exported via the CRM1-pathway . Since gammaretroviral pol seems to behave like a CTE the effect of Tpr knockdown was examined next. No significant change in Gag polyprotein levels was observed with Tpr knockdown in HEK293T cells (data not shown).
Little has been reported concerning post-transcriptional regulation of gammaretroviral genes. Here it was shown that Gag polyprotein synthesis is strictly dependent upon a 100 nucleotide element in pol RNA that resembles the CTE of the betaretrovirus MPMV. Like the MPMV CTE, the γ-CTE described here appears to recruit the cellular factor NXF1. Overexpression of either NXF1/NXT or of SRp20 serves to increase gammaretroviral Gag polyprotein production. While γ-CTE contributes to gag mRNA stability and export to the cytoplasm, it’s major effect is to promote polysome loading of the gag mRNA.
Cell lines, tissue culture, and drugs
HEK293T cells were grown in Dulbecco’s modified Eagle medium (D-MEM) (Invitrogen) supplemented with 10% fetal bovine serum (FBS). Puromycin was used up to a concentration of 8 μg/ml.
Oligonucleotides used in this study
NXF1 - + KD
Codon optimized XMRV gag
XMRV gag-pol fs has a mutation at the 5′ end of pol sequence that puts the pol sequence out of frame with respect to gag. BstEII digestion and subsequent fill-in of the 5′ overhang by T4 DNA polymerase generated a frameshift mutation at the 5′ end of the pol sequence with stop codons downstream that block pol translation.
XMRV gag was amplified with primers X01f and X15r as an NheI-NotI fragment. The codon optimized XMRV gag open reading frame was synthesized by GENEART AG (Regensburg, Germany) and inserted into the Nhe1 and Not1 sites of pcDNA3.1. The codon optimized gag sequence is shown in Table 1. It has 398 silent nucleotide changes with respect to the original gag sequence. The GC content of the codon optimized gag is 64%, as compared to 55% in the original.
The mapping of XMRV pol sequence required a construct that contained XMRVgag plus pol sequences downstream. The 5’ end of pol was amplified with primers X14f and X14r as a linker, and cloned NotI-XhoI downstream of XMRV gag. Additional restriction sites at the 3’ end were used to clone additional pol sequences downstream of XMRV gag. To map the activity of pol fragments unique NotI, KpnI, EcoRI, and XhoI sites were used to add and combine pol sequences downstream of XMRV gag. Initially XMRV pol was divided into three major fragments, encompassing nucleotides 2232-3457, 3457-5199, and 1611-2232. Fragment 2232-3457 was amplified with primers X04f and X04r and cloned KpnI-EcoRI downstream of XMRV gag. Fragment 3457-5199 was amplified with primers X20f and X20r and cloned EcoRI-XhoI downstream of XMRV gag. 1611-2232 was amplified with primers X07f and X07r and cloned KpnI-EcoRI downstream of XMRV gag. Fragments 2232-3457 and 3457-5199 were further truncated and cloned into XMRV gag with KpnI-EcoRI and EcoRI-XhoI, respectively. Fragment 2558-3456 was amplified with primers X08f and X04r, 2558-3307 was amplified with primers X08f and X09r, 2558-3155 was amplified with primers X08f and X10r, 2558-3007 was amplified with primers X08f and X11r, 2558-2858 was amplified with primers X08f and X12r, and 2558-2709 with primers X08f and X13r. 4715-5199 was amplified with primers X26f and X20r, and 4876-5199 was amplified with primers X27f and X20r.
Another construct included pol sequences from 4543 to 5199 and was used to mutate splice acceptor and donor sites. Potential splice donor sites were predicted using NetGene2 (http://www.cbs.dtu.dk/services/NetGene2/). The single point mutations in SA and SD were generated by overlapping PCR. We amplified two PCR products to mutate the SA site using primers X20f and X21r, and X21f and X20r. Primers X20f and X20r were used to fuse these two PCR products.
Moloney murine leukemia virus (MLV, accession number AF033811) gag-pol was generated by PCR in 3 fragments. The first fragment was cloned NheI-XhoI and the second fragment XhoI-EcorV, both into pcDNA3.1(-). The third fragment was cloned EcorV-NotI into pcDNA3.1(+). The first and the second fragment were subcloned as a NheI-EcoRV fragment into pcDNA3.1(+) that already contains the third fragment.
MLV gag-pol FS has a frameshift at the 5′ end of MLV pol. This frameshift was induced by cutting the 5′ end of pol sequence at the unique restriction site XcmI and made blunt-ended with the Klenow fragment of DNA polymerase. pcMLVgag encompasses MLV gag as an NheI-NotI fragment and MLV gag 2232-3457 with MLV pol fragment 2232-3457 cloned XhoI-XbaI downstream of MLV gag.
An NXF1-binding domain (γ-CTE) was identified within pol. The secondary structure of a 100 nucleotide pol fragment including a putative NXF1 binding domain was modeled using the mfold program (http://mfold.rna.albany.edu/?q=mfold/RNA-Folding-Form). The γ-CTE, multimerized four times or eight times, was synthesized by GenScript, and subcloned downstream of gag. 4x X-CTE TBD4 and 8x X-CTE TBD4 contains mutations of 4 nucleotides within the critical NXF1-binding domain. The plasmid HIVgp1xCTE was provided by Dr. Michael Malim.
RNA from 293 T cells was reverse transcribed from RNA to complementary DNA (cDNA) by standard methods. The specific primer T01r was applied to obtain NXF1 cDNA and the T03r to obtain NXT2 cDNA. We amplified the cDNA with primers T01f and T01r to clone NXF1 XbaI-NotI into pcDNA3.1(-) Strep/Flag. The primers T02f and T02r were used to amplify NXT2 and clone it NheI-NotI into pcDNA3.1(-) Strep/Flag. The primers T03f and T03r were used to amplify SRp20, and the primers T06f and T06r to amplify SRp40. The primers T04f, T05f and T05r were used to amplify the two mutants SRp20ΔRRM and SRp20R3A, respectively.
For the knockdown vector cloning we used an shRNA design program (http://cancan.cshl.edu/RNAi_central/RNAi.cgi?type=shRNA). Three miRNA-based shRNA targeting sequences were designed against the NXF1/TAP transcript. Three 97-mer oligonucleotides were synthesized and PAGE purified, NXF1-1, NXF1-2, and NXF1-3 (sequences in Table 1), The 97-mer oligonucleotides were then amplified by PCR using primers: miR-30f and miR-30r. The PCR reaction was carried out with AccuPrime Pfx SuperMix, 1 mol/L Betaine (Sigma-Aldrich), 0.4 μmol/L each primer, and 100 ng 97-mer oligonucleotide template. The PCR product was column purified, digested with XhoI and EcoRI, and ligated in pAPM to create the pAPM-NXF1 knockdown constructs (NXF1-1, NXF1-2 and NXF1-3). The function of each NXF1 KD vector was checked by generating stable, puromycin-selected HEK293T cells. Efficiency of the NXF1 knockdowns was assessed by western blot for NXF1. NXF1-3 was selected for further experiments since it gave the most potent knockdown.
Generation of NXF1 KD cells and rescue of the NXF1 protein
To generate stable microRNA-based shRNA KDs, HEK293T cells were transduced with pAPM microRNA-based shRNA vectors targeting either control or NXF1 mRNA (NXF1-1, NXF1-2, and NXF1-3). We transfected HEK293T cells with a DNA-mix containing 2.0 μg of pAPM (that contains the 97mer oligo), 1.4 μg of psPAX2 (packaging vector), and 0.7μg of pMD2G (envelope) on day 1. Then we collected the supernatent and filtered it through a 0.45 μm syringe filter. We removed 800 μl medium per well of the prepared HEK293T cells (6-well plate) and then added 800 μl of the supernatant (containing the virus) per well. 12 hrs after transduction, the cells were transfected with the plasmids of interest, using the Calcium Phosphate. 7 hrs after transfection, the cells were selected with 8 μg/ml puromycin. To generate the NXF1 rescue cells, HEK293T NXF1 KD and control KD cells were co-transfected with the plasmid of interest and the pcNXF1 expression vector, respectively. 7 hours after transfection, the cells were selected with 8 μg/ml puromycin for 36 hours and assayed for KD and protein rescue by SDS-PAGE⁄ western blot.
Standard PCR reactions were performed using either the High fidelity PCR system (Roche) or the AccuPrim Pfx system (Invitrogen), using specific primers at 0.2 μM and 0.4 μM, respectively. The template for the PCR reaction was either a plasmid or cDNA. The PCR reaction was set up according to the manufacturer′s instructions in Eppendorf Mastercycler.
Oligonucleotides used for qRT-PCR
After isolating total and cytoplasmic RNA the RT-PCR reaction was set up using 100 ng of template per reaction, 0.1 μM of each primer pair, and 2 x reaction buffer. Thermal cycler conditions used were 20 min RT reaction at 42°C, 5 min hot-start Taq activation at 95°C and 35 cycles of amplification. Each amplification cycle was composed of 5 s denaturation at 95°C, 5 s annealing at 58°C, 15 s extension at 72°C, 7 s acquisition at 83°C, using the lightcycler; 5 sec denaturation at 95°C, 5 sec annealing at 55°C, 20 sec extension at 72°C, 11 sec acquisition at 83°C. RT-PCR reactions were performed using the CFX96 thermal cycler (Biorad).
Antibodies used in this study
Rat anti-p30 hybridoma
NIH AIDS Research and Reference Reagent Program, contributed by Bruce Chesebro
Rat Anti-SSFV Env (7C10)
Provided by Dr. Sandra Ruscetti, NCI
Mouse Anti-TAP (NXF1)
BD Transduction Laboratories
Anti-rat HRP conjugated
Santa Cruz Biotechnology
Anti-mouse HRP conjugated
Santa Cruz Biotechnology
HEK293T cells were trypsinized and harvested 48 h after transfection. Subsequently cells were washed twice with PBS. 3.5 × 106 cells were resuspended in 175 μl of lysis buffer (50 mM Tris-HCl pH 8.0, 140 mM NaCl2, 1.5 mM MgCl2, 0.5% NP40, 1 mM DTT, 1000 U/ml RiboLock Fermentas) and incubated on ice for 5 min. The lysed cells were pelleted by centrifugation at 500 × g at 4°C for 5 min. Afterwards the supernatant was transferred to a new tube. We added 600 μl RLT Buffer (RNeasy MINI Kit, Qiagen). Then we added 430 μl to the homogenized lysate. 700 μl of the sample were transferred to an RNeasy spin column, and centrifuged for 15 s at ≥8000 × g. This step was repeated with the remaining sample. We added 500 μl RW1 to the RNeasy spin column and centrifuged again for 15 s at ≥8000 × g. Then we added 500 μl Buffer RPE twice to wash the spin column membrane for 15 s at ≥8000 × g and 2 min at ≥8000 × g, respectively. Then we placed the RNeasy spin column in a new 2 ml collection tube and centrifuged at full speed for 1 min. Finally we placed the RNeasy spin column in a new 1.5 ml collection tube and added 30 μl RNase-free water directly to the spin column membrane, and centrifuged for 1 min at ≥8000 × g to elute the RNA.
An RNA probe was generated by cloning XMRV gag into pBS KS(+). The plasmid was linearized using KpnI and incubated with T7 RNA polymerase and (α-32P-UTP) for two hours at 37°C using a protocol provided with the T7 RNA Polymerase (Fermentas).
Total and cytoplasmic RNA were separated on an agarose-formaldehyde gel (1x MOPS, 3,7% formaldehyde, 1% agarose) for 4 hrs at 90 V, 60 mA. As a marker the RiboRuler™ High Range RNA Ladder, 200-6000 bases (Fermentas), was used. We added 2x RNA loading dye containing ethidium bromide to stain the RNA. After electrophoresis the gel was incubated in 0.05 N NaOH buffer for 10 min. The gel and Hybond N Nylon membrane (Amersham) were then equilibrated in 0.5× TBE buffer for one hr. The transfer was performed in a Trans-Blot Semi-Dry Electrophoretic Transfer Cell (Biorad) for 35 min (3 mA/cm2). RNA crosslinking was conducted with a Stratalinker. Subsequently the membrane was hydrated in RNase-free water before adding the prehybridization buffer for two hrs at 68°C. Hybridization was performed by adding the (α-32P-UTP) labeled RNA probe for 16 hrs at 68°C. Afterwards the membrane was washed three times. The first wash (0.5 x SSC, 1% SDS) was at room temperature whilst the second and third washes (1× SSC, 1% SDS) were at 68°C. Blots were exposed to film and subsequently quantified on a Phosphorimager Typhoon FLA 7000 using the Image Quant TL analysis software.
48 h post-transfection of 2 × 107 293 T cells, cycloheximide was added to the tissue culture medium (50 μg/ml final concentration) for 5 min. The cells were then harvested by trypsinization and washed twice with cold PBS. Both the trypsin solution and the PBS also contained 50 μg/ml cycloheximide. The cell pellet was then resuspended in 400 μl of cold lysis buffer (50 mM Tris-HCl at pH 7.4, 100 mM KCl, 1.5 mM MgCl2, 1 mM DTT, 1 mg/ml Heparin, 1.5% NP40, 100 μg/ml cycloheximide, protease inhibitor cocktail Roche 100 μl/ml, 100 U RiboLock Fermentas), and incubated for 15 min on ice before centrifuging for 10 min at 12,000 × g at 4°C to remove the nuclei. The supernatant was loaded onto gradients of 15% to 55% sucrose (w/v). After ultracentrifugation in a Beckman SW41 rotor for 3 hrs at 210,000 × g at 4°C, the gradient was analyzed using an ISCO UA-6 collector. 2 μl of 20 μg/μl glycogen was added to each fraction which were then extracted with TriReagent as follows: after incubation on ice for 15 min, 300 μl of chloroform was added followed by centrifugation for 2 min at 12,000 × g. The upper phase was collected into a new tube and 700 μl of isopropanol was added. The mixture was stored for 30 min at −20°C. The RNA was pelleted at 12,000 × g for 10 min at 4°C. The supernatant was removed and the RNA was washed twice with 1 ml of ethanol. After removing the ethanol and air drying the pellet, the RNA pellet was resuspended in 20 μl of RNase-free H2O. After DNAse I (NEB) treatment, 100 ng was then used as template in the reverse transcriptase-polymerase chain reaction (RT- PCR).
HB and JL conceived and designed the experiments and wrote the paper. HB performed the experiments. HB and JL analyzed the data. Both authors read and approved the final manuscript.
We thank Bruce Chesebro, Joseph Curran, Michael Malim, Sandra Ruscetti, Michel Strubin, and Laurent Roux for technical guidance and for reagents. This work was supported by NIAID/NIH/USA grant RO1AI59159, NIDA/NIH/USA Grant DP1DA034990, and Swiss National Science Foundation grant 3100A0-128655 to J.L. The funders had no role in study design; in the collection, analysis, and interpretation of data; in the writing of the manuscript; or in the decision to submit the manuscript for publication.
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