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Figure 3 | Retrovirology

Figure 3

From: Gammaretroviral pol sequences act in cis to direct polysome loading and NXF1/NXT-dependent protein production by gag-encoded RNA

Figure 3

Either of two pol fragments promote Gag protein production. HEK293T cells were transfected with the indicated constructs and cell lysate was harvested 48 hrs later. (A) Schematic of the XMRV pol sequence fragments that were cloned out of frame and downstream of XMRV gag in which the natural UAG stop codon was replaced with UGA. (B) Cell lysate was probed with anti-CA antibody (upper panel) and anti-β-actin antibody (lowel panel).

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