Figure 2
Protein synthesis by pol is not required to promote Pr65Gagprotein production. HEK293T cells were transfected with the indicated constructs (XMRV gag-pol, XMRV gag, XMRV gag-pol with a frameshift mutation just after the XMRV gag stop codon, codon optimized XMRV gag, or empty vector), and harvested 48 hrs later. (A) Schematic representation of XMRV constructs showing interruption of pol translation by introduction of a frameshift mutation just after the stop codon of XMRV gag. (B) HEK293T cell lysate was probed with anti-CA antibody (upper panel) and anti-β-actin antibody (lowel panel). (C) Virus-like particles (VLPs) pelleted from the supernatant by ultracentrifugation were collected and analyzed by immunoblotting with anti-CA antibody.