Figure 8

MPMV CTE and NXF1/NXT promote gammaretroviral Gag protein production. HEK293T cells were transfected with plasmids expressing gag-pol, gag, or gag with the 2232-3456 pol fragment. In addition, cells were transfected with a plasmid in which a single MPMV CTE was cloned downstream of gag (A), or cells were co-transfected with expression plasmids for NXF1 and NXT, as indicated in (B). Cell lysate was harvested 48 hrs later and probed with anti-CA antibody (upper panel) or anti-β-actin antibody (lowel panel). Cell lysate from (B) was probed additionally with anti-NXF1 and anti-NXT antibody, as indicated. Similar results were obtained with identical constructs from MLV and XMRV. A representative experiment with MLV derived constructs is shown.