Figure 10
The effect of NXF1 KD on Pr65Gagprotein production. (A) Diagram showing protocol for short-term selection of cells with NXF1 KD. HEK293 cells were transduced with lentiviral vectors bearing miR30 frameworks targeting either luciferase or NXF1. Then, cells were transfected with the indicated plasmids, and finally selected in pools with puromycin. (B) 55 hrs after addition of puromycin cell lysate was probed by western blot with anti-gammaretrovirus CA antibody, anti-HIV-1 CA antibody, anti-NXF1 antibody, or anti-β-actin antibody, as indicated. Similar results were obtained with identical constructs from MLV and XMRV. A representative experiment with MLV derived constructs is shown.