Figure 1

Gammaretroviral pol sequence is required for efficient Pr65^Gagprotein production. (A) HEK293T cells were transfected with the indicated XMRV and MLV expression constructs. Cell lysate was harvested 48 hrs later and probed with anti-CA antibody (upper panel) or anti-β-actin antibody (lowel panel). (B) The magnitude difference in XMRV Pr65^Gag protein level in cells tranfected with the the gag-pol or gag-only expression plasmids was determined by comparing the gag-only signal with serial dilutions of lysate from cells transfected with gag-pol.