- Open Access
Inhibition of HIV-1 integrase nuclear import and replication by a peptide bearing integrase putative nuclear localization signal
- Aviad Levin†1,
- Ayelet Armon-Omer†1, 4,
- Joseph Rosenbluh1,
- Naomi Melamed-Book1,
- Adolf Graessmann2,
- Elisabeth Waigmann3 and
- Abraham Loyter1Email author
© Levin et al; licensee BioMed Central Ltd. 2009
- Received: 30 September 2009
- Accepted: 05 December 2009
- Published: 05 December 2009
The integrase (IN) of human immunodeficiency virus type 1 (HIV-1) has been implicated in different steps during viral replication, including nuclear import of the viral pre-integration complex. The exact mechanisms underlying the nuclear import of IN and especially the question of whether it bears a functional nuclear localization signal (NLS) remain controversial.
Here, we studied the nuclear import pathway of IN by using multiple in vivo and in vitro systems. Nuclear import was not observed in an importin α temperature-sensitive yeast mutant, indicating an importin α-mediated process. Direct interaction between the full-length IN and importin α was demonstrated in vivo using bimolecular fluorescence complementation assay (BiFC). Nuclear import studies in yeast cells, with permeabilized mammalian cells, or microinjected cultured mammalian cells strongly suggest that the IN bears a NLS domain located between residues 161 and 173. A peptide bearing this sequence -NLS-IN peptide- inhibited nuclear accumulation of IN in transfected cell-cycle arrested cells. Integration of viral cDNA as well as HIV-1 replication in viral cell-cycle arrested infected cells were blocked by the NLS-IN peptide.
Our present findings support the view that nuclear import of IN occurs via the importin α pathway and is promoted by a specific NLS domain. This import could be blocked by NLS-IN peptide, resulting in inhibition of viral infection, confirming the view that nuclear import of the viral pre-integration complex is mediated by viral IN.
- Green Fluorescence Protein
- Yeast Cell
- Nuclear Import
- 2LTR Circle
- Nuclear Localization Signal Sequence
Active nuclear import begins in the cytoplasm with recognition of the transported cargo molecules by nuclear transport receptors designated as importins . Proteins targeted to the nucleus contain a specific amino acid sequence, termed nuclear localization signal (NLS), which is recognized by either a member of the importin α family, or directly by importin β. The resultant complex then interacts with the nuclear pore complexes (NPCs), through which it is subsequently transported into the nucleus . This nuclear translocation machinery is highly conserved among lower and higher eukaryotes .
Human immunodeficiency virus type 1 (HIV-1) belongs to the lentivirus family, which in contrast to other retroviruses can infect terminally differentiated cells [4, 5]. The capability of HIV-1 to infect cell-cycle arrested cells has been ascribed to the ability of its pre-integration complex (PIC) [6, 7] to translocate across the nuclear envelope via the NPC . The karyophilic properties of the viral PIC have been attributed mainly to three viral proteins: matrix (MA), Vpr, and integrase (IN) [8–10]. The cellular Lens Epithelium-Derived Growth Factor p75 (LEDGF/p75) protein as well as the DNA flap structure of the viral cDNA have also been implicated in promoting the translocation of the PIC into nuclei of virally infected cells [11–13]. Yamashipa et al. have proposed that the HIV capsid protein plays a crucial role in controlling the nuclear import of the HIV genome . However, despite these extensive studies and numerous reports, the nuclear import mechanism of the PIC and the involvement of viral or cellular factors driving such a process remain unclear and controversial .
The HIV-1 IN protein consists of 288 amino acids and three functional domains: the N-terminal domain (residues 1-50), which bears a zinc-binding motif [16, 17]; the central core domain (residues 51-212), which includes the catalytic DDE motif [18–20]; and the C-terminal domain (residues 213-288), which has been shown to non-specifically bind the DNA [19–21]. To achieve integration of the viral DNA into the host chromosome, the IN must be translocated into the nuclei of infected cells .
Various studies have showed that IN is a karyophilic protein. Transfection of cultured mammalian cells with expression vectors bearing IN results in nuclear accumulation of the encoded protein . Import of fluorescently labeled IN into the nuclei of digitonin-permeabilized mammalian cells was shown to be ATP- and temperature-dependent; and this import could be blocked by the addition of unlabeled IN, clearly indicating an active, receptor-mediated process [23, 24]. Based on the ability of recombinant IN protein to bind in vitro to importin α and the ability of a peptide bearing the prototypic simian virus 40 T-antigen NLS (SV40-NLS) to block such binding, as well as nuclear import, nuclear transport of IN has been suggested to occur via the importin α pathway [8, 23]. Moreover, interaction of IN with the importin α family has recently been reported .
The possibility of the IN protein being carried into the cell's nuclei by other cellular components has also been suggested [13, 26, 27]. The LEDGF/p75 was initially implicated in mediating the nuclear import of IN . However, studies on the specific contributions of LEDGF/p75 demonstrated that it facilitates the interaction between IN and nuclear chromatin, but is not directly involved in the import process . An interaction with importin 7, via a sequence located at the C terminus of IN , has been proposed. However conflicting results have been obtained regarding the necessity of this receptor [29, 30]. Furthermore anti-importin 7 antibodies did not block nuclear import of IN . More recently, the involvement of the transportin-SR2 (TNPO3) in the nuclear import of IN has been suggested . This conclusion is based mainly on experiments showing that the knockdown of transportin-SR2 (TNPO3) resulted in the reduction of nuclear cDNA .
In the present work, we further confirm and emphasize the role that importin α plays in promoting nuclear import of the viral IN and thus in virus infection. Multiple approaches and various experimental systems such as transfected mammalian and yeast cells as well as virally infected cells have been used to answer the question of whether nuclear import of IN may be mediated by its own NLS via interaction with importin α. Our results clearly demonstrate that IN accumulates within wild-type yeast cell nuclei, but fails to do so in importin α-defective yeast mutants (srp1-31) . A full-length IN, as well as a peptide bearing the IN amino acid sequence 161-173 (NLS-IN), interacted in vivo with mammalian importin α, as demonstrated by a bimolecular fluorescence complementation (BiFC) assay  in yeast. The involvement of amino acids 161-173 in mediating nuclear import of IN was also demonstrated by microinjection and transfection experiments in cultured mammalian cells. Furthermore, the putative NLS-IN peptide inhibited nuclear accumulation of IN as well as of cDNA in IN-transfected and virally infected cells. This appears to be due to the ability of the NLS-IN peptide to compete for the interaction between the viral IN and the cellular importin α. This peptide has also been found to significantly inhibit HIV-1 replication in TZM-bl cells and inhibit the integration of viral cDNA in infected cells. Thus, the present results support our  and others' previous results  claiming that the IN protein contains a specific functional NLS sequence, which is located between amino acids 161 and 173 and which confers to this protein the karyophilic property required to ensure productive viral infection.
The NLS-IN peptide is functional in transfected and microinjected mammalian cells as well as in yeast cells
Essentially similar results were obtained when the degree of nuclear import was quantitatively estimated using an ELISA-based system and biotinylated BSA (Bb) conjugates (Fig. 3F). A relatively high degree of nuclear import was observed with both Bb-NLS-IN and Bb-IN, whereas almost no import was observed with Bb-152-IN (Fig. 3E), again emphasizing the role of NLS-IN in mediating nuclear import of IN.
NLS-IN mediates binding to importin α in yeast cells and in vitro
NLS-IN inhibits IN and cDNA nuclear import as well as HIV-1 replication in cultured cells
In the light of the results showing the requirement of NLS-IN for nuclear import of IN in mammalian as well as in yeast cells, it became of relevance to study its effect in virally infected cells.
Inhibition of nuclear import of the viral IN in HIV-1 infected cells as well is evident from the immunofluorescence microscopic study shown in Fig. 7B. From the immunostaining results, it appears that in infected cells, the IN is localized both within the cytosol and within the nuclei (Fig. 7B (no peptide)). However, no intranuclear fluorescence was observed in cells treated with the NLS-IN-Pen or the SV40-NLS-Pen peptides, indicating the inhibition of nuclear import (Fig. 7B). In contrast, some intranuclear fluorescently labeled IN could be observed when the infected cells were incubated in the presence of the SV40-mut-NLS-Pen or the Pen peptide itself (Fig. 7B). This is also evident from the fact that more cytosolic IN was present in such peptide-treated cells than in those incubated in the absence of any peptide or with the non-active peptides (Fig. 7B).
The question of how retroviruses and particularly HIV-1 cross the nuclear envelope in cell-cycle arrested cells is of specific scientific interest. After long and extensive research, it appears that no clear mechanism has yet emerged and the possibility that several pathways simultaneously mediate nuclear import of the viral PIC cannot be excluded. In the present work-following our previous experiments using in vitro systems -we focused on the nuclear import of IN protein using yeast and mammalian cells, as well as on the contribution of its putative NLS-IN  on the HIV-1 replication process.
An interaction between HIV-1 IN and importin α was first demonstrated by Gallay et al. . Similarly an interaction between the IN and members of the importin family and that its nuclear transport appears to be dependent on the importin α/β heterodimer have also demonstrated by Hearps and Jans . A functional NLS sequence was identified between amino acids 161 and 173 of the IN protein whose mutation disrupted IN translocation into nuclei [12, 42]. However, later studies indicated that this sequence may be required for promoting viral DNA integration and not necessarily nuclear import . However, our previous work clearly demonstrated that a peptide bearing IN 161-173 residues can mediate import of a conjugated protein into the nuclei of permeabilized cells, confirming the view that it can function as a NLS .
In the present work, we have further studied the involvement of IN amino acids 161-173 in promoting its nuclear import. Nuclear accumulation was observed in yeast transformed with an expression vector bearing only the NLS-IN sequence, which in order to avoid diffusion into the nuclei, was fused to two molecules of GFP, resulting in a molecule of about 58 kDa. It is assumed that molecules of up to about 30 to 40 kDa can passively diffuse via the NPCs into cell nuclei . Therefore, the nuclear import observed here with the various GFP-IN conjugates, the molecular weights of which varied between 48 and 60 kDa, should be ascribed to a receptor-mediated, active process. Nuclear import was practically not observed when yeast cells were transformed with the 152-IN truncated protein, which lacks the putative NLS sequence. Results obtained in permeabilized or microinjected cells, as well as in IN-transfected intact mammalian cells, further supported these results. The failure of 152-IN to penetrate the cell nuclei suggests that if an additional NLS sequence, besides the one located between 161 and 173, were present, it must be located closer to the C terminus of the IN protein, a possibility that has been suggested previously [25, 44]. However, the fact that the truncated 180-IN protein was translocated into the cell nuclei indicates that the identified NLS-IN is sufficient to provide the IN with karyophilic properties. Our results demonstrating inhibition of IN nuclear import in transfected cells by a peptide carrying the NLS-IN sequence further emphasize the view that this sequence gives IN its karyophilic properties.
SRP1 is the only known importin α protein in budding yeast, and previous studies have demonstrated that it is essential for proper maintenance of nucleocytoplasmic trafficking . Indeed, a temperature-sensitive mutant in SRP1 has been isolated (srp1-31) and shown to be defective in nuclear import at the non-permissive temperature . Thus, yeast, including SRP1-mutated strains, has been instrumental in studying various aspects of the nuclear import machinery [36, 46] and in characterizing karyophilic properties. The availability of the temperature-sensitive srp1-31 mutant offers an advantage for studying nuclear transport of the IN protein in yeast cells. The involvement of importin α in the IN nuclear import pathway can be inferred from our experiments showing nuclear import at the permissive, but not at the non-permissive temperature where importin α is inactivated .
A direct and specific interaction between IN or the NLS-IN sequence and the mammalian importin α in vivo, within the intracellular environment, was demonstrated using the BiFC assay. The view that restoration of GFP fluorescence using the BiFC assay in yeast cells results from a specific protein-protein interaction has already been established [47, 48]. Indeed, our results clearly demonstrated the well-known IN-IN and IN-LEDGF/p75 interactions in yeast cells. Next we showed restoration of fluorescence in yeast cells expressing the combination of importin α and the full-length IN or the truncated 180-IN. The combination of importin α and the truncated 152-IN protein did not result in the appearance of fluorescence, strongly indicate that the NLS-IN sequence is located between amino acids 152 and 180, and is necessary to mediate the interaction with the nuclear receptor. However, our results do not exclude the possibility that additional NLS sequences are located between amino acid 180 and the C terminus, as suggested previously [25, 44]. Attempts to study the interaction of fragments bearing these regions failed due to non-specific restoration of fluorescence (not shown). A similar pattern also characterized the in vitro interaction of the various INs. Our co-IP experiments confirmed the interaction between IN and importin α in virally infected cells. Furthermore, the view that such an interaction is mediated by the putative NLS-IN was supported by the results showing disruption of this interaction by the cell permeable NLS-IN or SV40-NLS peptides.
The NLS-IN-Pen peptide almost totally blocked viral infection of TZM-bl cells, caused high inhibition of the cDNA integration process, and affected p24 production when added up to 18 h post-infection (PI). Inhibitory effects were much higher in cell-cycle arrested than in dividing cells, clearly supporting the notion that the NLS-IN-Pen bears a sequence which is involved in mediating nuclear import of the IN. Inhibition of IN as well as of viral cDNA nuclear import by the NLS-IN-Pen peptide was demonstrated directly in the present work using immunofluorescence staining of IN and quantitative estimation of nuclear viral DNA.
The amino acid residues of NLS-IN described in the present work have been implicated in additional viral-related functions, such as specific binding of IN to the LEDGF/p75 protein and to the viral LTR region [38, 49, 50]. Due to its multifunctional activity, this region may be useful as a target for the development of inhibitors. As mentioned above our present as well as previous results describing the involvement of importin α in mediating nuclear import of IN do not exclude the possibility of an additional nuclear import pathway for IN in which TNPO3 is involved.
Mammalian, bacterial and yeast cells
Monolayer adherent HeLaP4 and HeLa TZM-bl cells (obtained through the NIH AIDS Research and Reference Reagent Program) expressing the β-galactosidase gene under regulation of the transactivation response element  were grown in Dulbecco's modified Eagle's medium. COS-7 and Colo-205 mammalian cells and the T-lymphocyte cell lines H9 and Sup-T1 were grown in RPMI 1640. All media were supplemented with 10% (v/v) fetal calf serum, 0.3 g/l L-glutamine, 100 units/ml penicillin, and 100 units/ml streptomycin (Biological Industries, Beit Haemek, Israel). Cells were incubated at 37°C in a 5% CO2 atmosphere and re-cultured every 4 days. HeLaP4/IN-expressing cells were generated by stable transfection into HeLaP4 cells  of pcDNA3.1 plasmid bearing the full wt IN gene. Selection was carried out for four weeks with 400 μg/ml Hygromycin B. Escherichia coli strain DH5α served as the host for general plasmid construction and maintenance, and E. coli strain BL21 (DE3) was used for protein overexpression. The yeast strains were congenic to Saccharomyces cerevisiae W303. The W303 (MAT a, leu2-3, 112 trp1-1 ura3-1 ade2-1 his3-11,15) and srp1-31 (MAT a, leu2-3, 112 trp1-1 ura3-1 ade2-1 his3-11,15, srp1-31) strains were kind gifts from G. Fink (Whitehead Institute, Massachusetts Institute of Technology, USA). The EGY48 strain (MAT a, his3-11,15, trp1-1, ura3-52, leu2::LexA6op-LEU2) was a kind gift from Y. Gafni (The Volcani Center, Israel). Yeast strains were grown in yeast peptone dextrose-rich medium (2% peptone, 2% glucose, 1% yeast extract, w/v). Transformation was performed by the lithium acetate method [53, 54] and then the yeast cells were grown in standard yeast nitrogen (YNB) minimal media, prepared by adding the appropriate amino acids to 0.67% (w/v) YNB without amino acids (Difco) and supplemented with 2% glucose or 2% galactose (w/v) as carbon sources.
Wild-type HIV-1 was generated by transfection of HEK293T cells with pSVC21 plasmid containing the full-length HIV-1 HXB2 viral DNA. Wild-type viruses were harvested from HEK293T cells 48 and 72 h post-transfection. The viruses were stored at -75°C.
Virus stock titration
Quantitative titration of HIV-1 was carried out using the MAGI assay, as described by Kimpton and Emerman . Briefly, TZM-b1 cells were grown in 96-well plates at 1 × 104 cells per well. the cells were infected with 50 μl of serially diluted virus as described . Two days post-infection (PI), cultured cells were fixed and β-galactosidase was estimated exactly as described previously . Blue cells were counted under a light microscope at 200× magnification.
Synthesis of peptides
Peptides were synthesized on Rink amide resin using a model 433A Applied Biosystems peptide synthesizer with FastMoc chemistry, exactly as described previously . The Pen peptide had the following sequence: RQIKIWFQNRRMKWKK (Ant 43-58) .
All of the plasmids used in this study were constructed using PCR cloning techniques with the high-fidelity enzyme Platinum Pfx DNA polymerase (Invitrogen). Clones were subjected to automated DNA sequencing.
1. Site-directed mutagenesis to create a stop codon at position 152 or 180
The plasmid pT7-7-IN  was used as the template for IN mutagenesis. A QuikChange site-directed mutagenesis kit (Stratagene) was adapted to create an in-frame stop codon at the desired position within the IN sequence, according to the manufacturer's protocol. The mutagenic primers were designed to contain a stop codon after residues 152/180 of the IN giving pT7-7-152-IN/180-IN, respectively, as well as a new DraI site, to facilitate screening. The primers used were:
2. Construction of plasmids for GFP in yeast cells
The yeast expression cloning plasmid pYES2yEGFP with the GFP codon optimized (a kind gift from T. Gilon, Alexander Silberman Institute, Israel) was linearized with the BsrGI restriction enzyme at the stop codon site of the yEGFP sequence , and then dephosphorylated and purified. The DNA products of IN, 152-IN, and 180-IN were obtained by PCR amplification from the pT7-7-152-IN/180-IN, respectively. The primers used were:
IN-BamHI, BsrGI: 5'-GCCGGATGTACAGGATCCCCGGGCGCG-3'
These DNA products were then cloned into the BsrGI sites of the linearized pYES2yEGFP, resulting in the formation of pYES2yEGFP-IN (GFP-IN), pYES2yEGFP-152-IN (GFP-152-IN) and pYES2yEGFP-180-IN (GFP-180-IN). A GFP2-NLS-IN (amino acids 161 to 173 of IN) expression vector was prepared by PCR amplification from pT7-7-180-IN. The primers used were: 5'BsrGI: 5'-CCGCCATGTACAAAGAGCTCAAAAAAATCATCGGTCAG-3' 3 BsrGI: 5'-GCGGTATGTACACCAGCAGAGTAACCACCGATAC-3' The resultant DNA products were cloned into pBS-yEGFP, resulting in pBS-yEGFP-NLS-IN, which was digested by SalI and BamHI, and the resultant product was subcloned into pYES2yEGFP to give pYES2yEGFP(2)-NLS-IN (GFP2-NLS-IN).
3. Construction of expression vectors for the BiFC assay
The yeast multicopy shuttle vectors pRS423 (with HIS3 as the selective marker) and pRS426 (with URA3 as the selective marker), both with the ADH1 promoter, were used as the cloning plasmids (a kind gift from D. Engelberg, Alexander Silberman Institute, Israel). The DNA coding region of the two GFP fragments , namely the N terminus (GN) and C terminus (GC), were cloned into pRS423 and pRS426  to give pRS423-GN and pRS426-GC, respectively. A linker consisting of (GGS)5 was used to separate the inserted genes and the GFP fragments. The coding sequences of IN and HIV-1 Rev  were amplified by PCR, digested by XmaI and NotI and inserted in-frame into the corresponding sites of pRS423-GN at the C-terminal fragments of the GN, resulting in pRS423-GN-IN (GN-IN) and pRS423-GN-Rev (GN-Rev), respectively. The PCR products of 152-IN and 180-IN were ligated into the SalI and SacII sites of pRS423-GN to give pRS423-GN-152/180 (GN-152-IN/GN-180-IN). The plasmid pRS423-GN-NLS-IN (GN-NLS-IN) was obtained by PCR amplification of the NLS-IN sequence, digested by SacI and EcoRI and ligated at the corresponding sites of pRS423-GN. The PCR products of IN, importin α (hSRP1-α), importin β and LEDGF were cloned into the corresponding XmaI and NotI sites of pRS426-GC, to construct the following plasmids, respectively: pRS426-GC-IN (GC-IN), pRS426-GC-mammalian importin α (GC-Impα), pRS426-GC-importin β (GC-Impβ) and pRS426-GC-LEDGF (GC-LEDGF, PCR amplification from pET28-LEDGF a kind gift from C.A. Casiano, Loma Linda University, USA). The sequences of all primers used in this work can be obtained directly from the authors.
Nuclear import of IN molecules in transformed yeast cells
Expression vectors bearing the GFP, GFP-IN, GFP-180-IN, GFP-152-IN, GFP2-NLS-IN and GFP-Pik1 coding regions under the galactose promoter (a generous gift from Dr. Thorner, UC Berkeley ) were introduced into the yeast strain W303. Expression was induced with 2% galactose for 4 h at 25°C. Following removal of the medium, the appearance of intracellular fluorescence was examined by confocal microscopy using an MRC 1024 confocal imaging system (Bio-Rad). Similarly, W303 and the srp1-31 yeast mutant were transformed by pYES2yEGFP-IN (GFP-IN). The strain srp1-31 contains a temperature-sensitive mutation in the SRP1 protein (importin α) [31, 61]. The transformed yeast cells were grown for 24 h at 25°C to reach the logarithmic phase, then divided into two cultures: one remained at 25°C (permissive temperature) and the other was transferred to 37°C (non-permissive temperature at which more than 95% of the SRP1 is inactivated ). After 4 h of growth, yeast cells were washed; GFP-IN expression was induced by the addition of galactose and the yeast cells were incubated for an additional 4 h at either 25 or 37°C. At the end of the growth period, yeast cells were harvested and then observed by confocal microscope. Yeast nuclei were identified by DNA staining in fixed yeast cells (4% v/v paraformaldehyde and 3.4% w/v sucrose) http://www.ciwemb.edu/labs/koshland/Protocols/MICROSCOPY/gfpfix.html with 4',6-diamidino-2-phenylindole (DAPI). To confirm expression of all proteins, western blot with anti-GFP antibody was performed. Each experiment was repeated at least three times.
Analysis of protein-protein interaction by the BiFC assay
In this approach, a molecule of GFP is separated into two portions: the N-terminal part (GN) ending at amino acid residue 154 and the C-terminal part (GC) beginning with the methionine residue preceding residue 155 of GFP . Neither of these two halves of GFP fluoresce when expressed alone; however, the fluorescence is restored when GN and GC are brought together as fusions with interacting proteins . The two halves of the GFP were cloned separately with each of the indicated proteins as described above and the different plasmids were transformed into the yeast strain EGY48. After 48 h at 30°C, the plates were transferred to 23°C for 3 days and then yeast cells were visualized by confocal microscopy. Nuclei were stained with DAPI as described above. Each experiment was repeated at least three times.
Expression and purification of the recombinant mammalian importin α (hSRP1-α) and HIV-1 IN proteins were performed essentially as described previously . The vectors, as well as the methods used to obtain purified recombinant truncated IN bearing amino acids 1-180 (180-IN) or amino acids 1-152 (152-IN) were the same as described for the full-length IN .
Nuclear import transport substrates
Proteins and peptides used in this work as transport substrates were covalently attached to either fluorescein isothiocyanate-labeled bovine serum albumin (FITC-BSA) or biotinylated BSA (Bb) molecules (Sigma). Sulfosuccinimidyl-4-(N-maleimidomethyl) cyclohexane-1-carboxylate (sulfo-SMCC) was used as the cross-linker to give FITC-BSA-protein/peptide and Bb-protein/peptide conjugates, as described previously .
Binding of transport substrates to importin α
Binding to importin α was estimated using an ELISA-based system essentially as described in [23, 63]. Briefly, Maxisorp plates (Nunc) were coated overnight at 4°C with a solution containing recombinant importin α (5 μg) in carbonate buffer (NaHCO3/Na2CO3 buffer at pH 9.6). All subsequent steps were as described previously .
Microinjection of fluorescently labeled transport substrates
FITC-BSA-IN, FITC-BSA-180-IN, FITC-BSA-152-IN, FITC-BSA-NLS-IN, as well as unlabeled FITC-BSA, were microinjected into the cytoplasm of cultured COS-7 cells as described previously . The CompInject AIS2 automated microinjection system (Cell Biology Trading, Hamburg, Germany) was used following the method developed by Graessmann and Graessman . Cultured mammalian cells were incubated at 37°C for 2 h after injection and observed by fluorescence microscopy.
Nuclear import in permeabilized mammalian cells: quantitative estimation
Import of Bb-IN proteins or of Bb-NLS-IN conjugates into nuclei of digitonin-permeabilized Colo-205 cells was determined quantitatively by an ELISA-based system, exactly as described previously .
Localization studies of transfected IN in cultured cells by immunostaining
HeLaP4/IN-expressing cells were grown on chamber slides (Nunc). After reaching 70-80% confluence, cells were arrested in the cell cycle by treatment with 5 μg/ml of aphidicolin, and then incubated with 150 μM of the indicated peptide for 6 h. Cells were fixed and immunostained essentially as described previously  with several modifications. Briefly, after fixation cells were blocked with 5% (w/v) BSA (IgG free) (Jackson) in PBS for 60 min. For detection of HIV-1 IN, cells were incubated with 1:100 rabbit α-IN (NIH AIDS Research & Reference Reagent Program, cat. no. 758) at room temperature for 60 min each. Cells were washed five times with PBS + 0.05% (v/v) Tween 20. Then the cells were incubated with a second antibody, Cy3-conjugated anti-rabbit antibody (Jackson) (1:200) at room temperature for 60 min followed by five washes with PBS + 0.05% Tween 20. For detection of DNA, cells were stained with DAPI according to the manufacturer's protocol. The slides were prepared with mounting media (Bio-Rad) and immunofluorescent cells were detected with a confocal microscope.
Localization studies of IN in HIV-1-infected cultured cells by immunostaining
HeLaP4 cells were grown on chamber slides. Cells were arrested in the cell cycle by treatment with 5 μg/ml aphidicolin, and then incubated with 150 μM of the indicated peptide for 2 h. After incubation with the peptides, cells were infected with wild-type HIV-1 at a multiplicity of infection (MOI) of 25. Cells were fixed and stained as described above with the following modifications: fixation was performed at 10 h PI, the first antibody was used at a dilution of 1:50, the second antibody at a dilution of 1:100.
Study of in vivoprotein-protein interactions using co-IP
Cells were infected with a MOI of 15 of the indicated viruses, harvested at 10 h post-infection (PI), washed three times with PBS and lysed by the addition of PBS containing 1% (v/v) Triton X-100. Half of the lysate volume was subjected to SDS-PAGE, then immunoblotted with either antiserum raised against IN amino acids 276-288 (anti-IN) (NIH AIDS Research & Reference Reagent Program cat. no. 758), anti-importin α (anti-Impα) antibody (Santa Cruz), or an anti-actin (anti-Actin) antibody (Santa Cruz) The complementary HRP-conjugated antibodies (Jackson) were used as the second antibody.
The remaining lysate or isolated fractions were incubated for 1 h at 4°C with either the anti-Impα or anti-IN antibodies. Following 3 h incubation with protein G-agarose beads (Santa Cruz) at 4°C, the samples were washed three times with PBS containing 1% (v/v) Nonidet P-40. SDS buffer was added to the samples and after boiling and running on an SDS polyacrylamide gel, the membranes were immunoblotted with either anti-Impα or anti-IN antibodies, and the complementary HRP-conjugated antibodies (Jackson) as second antibodies.
When peptides were used, cells were incubated with 150 μM of the indicated peptide for 2 h prior to infection.
HIV-1 titration by multinuclear activation of a galactosidase indicator (MAGI) assay
Quantitative titration of HIV-1 was carried out using the MAGI assay, as described previously . Briefly, TZM-b1 cells were grown in 96-well plates at 104 cells/well and incubated for 12 h at 37°C. Peptides were then added and after an additional 2 h of incubation, the cells were infected with 50 μl of serially diluted HIV-1. To obtain cell-cycle arrested cells, 5 μg/ml of aphidicolin was added 2 h before the experiment. Cultured cells were fixed 2 days PI and β-galactosidase was estimated . Blue cells were counted under a light microscope at 200× magnification.
Quantitative analysis of integration
Real-time PCR experiments were performed to estimate integration as described previously .
Sup-T1 cells were infected with wild-type HIV-1 at a MOI of 2, and the test compounds were added at different time points after infection (0, 2, 4,..., 24 h). Viral p24 production was determined at 48 h PI . Dextran sulfate was tested at 20 μM, AZT at 2 μM, NLS-IN-Pen, NLS-IN and Pen at 62.5 μM, LEDGF 402-411 at 12.5 μM .
Isolation of cytoplasm and nuclei from infected cells
The various fractions were obtained from virus-infected cells essentially as described previously [68, 69] with several modifications. Briefly, cells were harvested and washed twice in buffer A (20 mM Hepes pH 7.3, 150 mM KCl, 5 mM MgCl2, 1 mM DTT and 0.1 mM PMSF). Cells were then suspended in 200 μl of buffer A with 0.025% (w/v) digitonin and incubated at room temperature for 10 min. Cells were centrifuged for 3 min at 1000 g at room temperature. The supernatant was then centrifuged at 8000 g and separated into supernatant (cytoplasm) and pellet (nuclei) and stored at -70°C.
Quantitation of total and nuclear viral DNA
Total viral DNA was estimated using SYBR green real-time quantitative PCR at 10 h PI from the total or nuclear-isolated fractions of the infected cells. DNA was isolated by phenol chloroform method. Briefly, DNA samples (1 μg of DNA) were added to 95 μl containing 1× Hot-Rescue Real Time PCR Kit-SG (Diatheva s.r.l, Fano, Italy), and 100 nM of each PBS (primer-binding site) primer: F5 (5' primer, 5'-TAGCAGTGGCGCCCGA-3') and R5 (3' primer, 5-TCTCTCTCCTTCTAGCCTCCGC-3'). All amplification reactions were carried out using an ABI Prism 7700 Sequence Detection System (Applied Biosystems): One cycle at 95°C for 10 min, followed by 45 cycles of 15 s at 95°C and 35 s at 68°C. In each PCR run, three replicates were performed. All other details are exactly as described in Casabianca et al. .
Quantitation of 2LTR circles
Quantification of 2LTR circles was estimated exactly as described in Butler et al. .
The authors are grateful to J. Thorner (Berkeley) for providing us with the GFP-Pik1 plasmids. This work was supported by grants from the Israel Science Foundation (ISF; grant no. 888/05) and Jubiläumsfonds of the Austrian National Bank (Project No. 11462 to A. Loyter and EW).
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