Immunostaining experiments for intracellular localization of IN in transfected cells. HeLaP4/IN-expressing cells were generated by stable transfection into HeLaP4 cells using pcDNA3.1 plasmid bearing the full wt IN gene. Cells were fixed and immunostained using 1:100 rabbit a-IN and second antibody, Cy3-conjugated anti-rabbit antibody as described in Methods. Staining of IN (red) and DAPI (blue) was observed under confocal microscope. Bar 10 μm.