Sub-cellular localization of the full-length and truncated IN fused to GFP in transformed yeast cells. (A) Schematic presentation of the various expressed GFP-IN fusion proteins used in this experiment. (B) W303 yeast cells were transformed, using lithium acetate method, with expression vectors coding for the following: GFP-IN, GFP-180-IN, GFP2-NLS-IN, GFP-152-IN and GFP. Left panel, GFP fluorescence (green); middle panel, DAPI staining (blue); merged fluorescence is shown in the right panel. Bottom, a line profile through the overlay image showing that maximum GFP fluorescence and DAPI staining are co-localized (in the nucleus). Yeast cells were grown to exponential phase in selective minimal medium. After induction with galactose, cells were harvested and GFP fluorescence was observed under confocal microscope; all other conditions were as described in Methods. Bar 7 μm.