- Open Access
Peptide P5 (residues 628–683), comprising the entire membrane proximal region of HIV-1 gp41 and its calcium-binding site, is a potent inhibitor of HIV-1 infection
© Yu et al; licensee BioMed Central Ltd. 2008
- Received: 09 July 2008
- Accepted: 16 October 2008
- Published: 16 October 2008
The membrane proximal region (MPR) of the transmembrane subunit, gp41, of the HIV envelope glycoprotein plays a critical role in HIV-1 infection of CD4+ target cells and CD4-independent mucosal entry. It contains continuous epitopes recognized by neutralizing IgG antibodies 2F5, 4E10 and Z13, and is therefore considered to be a promising target for vaccine design. Moreover, some MPR-derived peptides, such as T20 (enfuvirtide), are in clinical use as HIV-1 inhibitors. We have shown that an extended MPR peptide, P5, harbouring the lectin-like domain of gp41 and a calcium-binding site, is implicated in the interaction of HIV with its mucosal receptor. We now investigate the potential antiviral activities of P5 and other such long MPR-derived peptides. Structural studies of gp41 MPR-derived peptides using circular dichroism showed that the peptides P5 (a.a.628–683), P1 (a.a.648–683), P5L (a.a.613–683) and P7 (a.a.613–746) displayed a well-defined α-helical structure. Peptides P5 inhibited HIV-1 envelope mediated cell-cell fusion and infection of peripheral blood mononuclear cells by both X4- and R5-tropic HIV-1 strains, whereas peptides P5 mutated in the calcium binding site or P1 lacked antiviral activity, when P5L blocked cell fusion in contrast to P7. Strikingly, P5 inhibited CD4-dependent infection by T20-resistant R5-tropic HIV-1 variants. Cell-cell fusion studies indicated that the anti-HIV-1 activity of P5, unlike T20, could not be abrogated in the presence of the N-terminal leucine zipper domain (LZ). These results suggested that P5 could serve as a potent fusion inhibitor.
- Antiviral Activity
- Long Terminal Repeat
- Envelope Glycoprotein
- Recombinant Peptide
In the vast majority of cases, HIV-1 transmission occurs at mucosal sites. The initial target cells for HIV-1 at mucosal sites include epithelial cells (CD4-negative) in simple monostratified mucosa (rectum, gastrointestinal tract, endo-cervix) and dendritic cells in pluristratified mucosa (vagina, exo-cervix, foreskin). Entry of HIV-1 into both types of cells is mediated by the cooperative interaction between both HIV-1 envelope subunits, gp120 and gp41, and galactosyl ceramide (GalCer) [1–3], thereby inducing HIV endocytosis in target cells and subsequent transcytosis or transfer to susceptible CD4+ T cells . We have previously demonstrated that the peptide P1 (a.a. 649–683) derived from the membrane proximal region (MPR) of gp41 acts as a galactose-specific lectin in binding to GalCer, the HIV-1 mucosal receptor expressed on both epithelial and dendritic cells [2, 4, 5]. In this case, HIV-1 neither fuses with nor infects target cells.
In contrast, CD4+ T cells are infected by HIV-1, leading to HIV spread. Infection is mediated by the HIV-1 envelope glycoproteins gp120/gp41, which trigger fusion between viral and cellular membranes, resulting in productive infection. Viral replication then causes rapid CD4+ T cell depletion, essentially at mucosal sites. Upon binding to CD4 and the co-receptor CCR5/CXCR4, gp120 undergoes serial conformational changes that allow the insertion of the gp41 fusion peptide into the target cell membrane and formation of the pre-hairpin structure. Subsequent formation of a hairpin structure (six-helix-bundle) promotes fusion between viral and cellular membranes [6, 7]. The hydrophobic region of the MPR plays an important role in this conformational change [8, 9]. The membrane fusion step can be inhibited by peptides mimicking the sequence of N-terminal (NHR) or C-terminal (CHR) heptad repeats, which block the association of the NHR and CHR regions, thus preventing hairpin formation .
In sum, the highly conserved MPR of gp41, which contains continuous epitopes recognized by broadly neutralizing antibodies 2F5 , 4E10  and Z13 , appears to be essential for both CD4-dependent target cell infection and CD4-independent mucosal entry of HIV-1. The MPR, along with the C-terminal cytoplasmic tail, is known to be determinant for envelope glycoprotein (Env) incorporation into virions and virus infectivity [9, 14].
In general, peptides from the CHR region (C-peptides) display higher inhibitory activity than peptides from the NHR region (N-peptides) . The first approved fusion inhibitor drug, Enfuvirtide (T20, a.a.640–673), displays an IC50 value in the nM range against some laboratory-adapted HIV-1 isolates in vitro, and excellent efficacy in clinical trials [16–18]. However, it leads in vivo to the generation of viral escape mutants, restricting its potential use for therapeutic purposes .
Peptide P1 is the minimal region of the MPR allowing interaction with GalCer. It contains three subdomains essential for its lectin activity, namely, the CHR which is rich in glutamic acid and highly negatively charged, the central hexapeptide ELDKWA epitope recognized by the potent and broadly neutralizing 2F5 IgG , and a hydrophobic tryptophan-rich region recognized by the other gp41-specific broadly neutralizing IgG, 4E10 and Z13 [12, 13]. Our recent biophysical studies  of peptides P1 and P5 (a.a.628–683), revealing an extended structure comprising not only the MPR peptide, but also the gp41 calcium-binding site (a.a.628–648) in its N-terminal portion , suggested that the affinity of gp41 for GalCer is dependent on the conformation of its lectin-binding site, which depends upon its environment. In particular, in the presence of calcium P5 undergoes a secondary structural change that decreases its affinity for GalCer.
In an effort to develop an effective microbicide, vaginal application of a peptide (C52L) similar to P5 , in combination with the CCR5 ligand CMPD167 or the entry inhibitor BMS378806, afforded protection against infection in Rhesus macaques upon vaginal challenge with SHIV.
As P1 and P5 comprise a larger portion of the MPR and were crucial for HIV entry in mucosa, we have evaluated in the present study their antiviral activity against HIV-1-mediated fusion and infection in comparison with T20, which comprises a more restricted region of the MPR.
Polymerase and deoxynucleoside triphosphates (dNTPs) for PCR amplification and isopropyl-beta-D-thiogalactopyranoside (IPTG) were purchased from Invitrogen. IgG sepharose was from Amersham Bioscience (Paris, France). Bacterial cells (E. coli Rosetta™) were from Novagen. The eukaryotic expression vector pGEV, designed for improving expression , was provided by Drs. F. Toma and P. Curmi (Evry-Val d'Essonne University, Evry, France). Materials used to purify PCR products were from QIAGEN (Paris, France). Ligase and restriction enzymes (NcoI and NotI) were from New England Biolabs, Inc (MA, USA). All other chemicals were of the highest purity commercially available (Sigma).
Chemically synthesized peptides P5 (628–683), P5 mutated in the calcium binding site (Δ Ca2+ bs-P5, 629–683), P1 (649–683) and LZ (560–594) were from NeoMPS (France) and Eurogentec (Belgium) with a purity >90%. Peptides T20 (# 9845) and N36 (546–581) (# 9773) were obtained from the AIDS Research and Reference Reagent Program, NIAID.
Construction, expression and purification of recombinant peptides
The coding sequence of all peptides was PCR amplified by use of a pair of synthetic oligonucleotides chosen in reference to the cDNA sequence of HIVHXB2. The PCR products were subsequently digested with NcoI/NotI and cloned into the pGEV vector, previously digested at the same sites. The sequences of recombinant constructs were confirmed by restriction enzyme analysis and DNA sequencing using the dideoxy chain termination method.
The recombinant plasmids pGEV-P1, P5, P5L and P7 bearing the transmembrane region (TM) were transformed into E. coli Rosetta™ (Novagen) competent cells by the heat shock procedure. One liter of LB medium (containing 100 μg/mL ampicillin) was inoculated with 5 mL of a fresh overnight culture and incubated at 37°C. The cultures were induced by the addition of 1 mM isopropyl-β-D-thiogalactopyranoside (IPTG) at an OD600 of 0.5–0.7, and further incubated for 3 h or overnight, under the same conditions. The cells were then harvested by centrifugation at 6,000 rpm for 20 min at 4°C. The pelleted cells were suspended in 100 mL of TST Buffer (50 mM Tris, 150 mM NaCl, 0.05% Tween-20; pH = 7.6), and sonicated. Cell debris was eliminated by centrifugation at 10,000 × g for 20 min. The soluble fraction was incubated for 2 h at 4°C in 5 ml of IgG-Sepharose that had been equilibrated in Buffer TST. The beads were washed once with 10 column volumes of TST Buffer, and subsequently 3 times with 10 column volumes of washing buffer (5 mM NH4Ac, pH = 5.0). Peptides were then eluted with 0.5 M CH3COOH, pH = 3.3 and immediately dialyzed against water or PBS, overnight.
Reverse Phase Chromatography
Reverse phase high performance liquid chromatography (RP HPLC) was performed using a C18 column. Peptides were eluted with buffer A (0.1% trifluoroacetic acid, 5% acetonitrile) for 15 min followed by a linear gradient from 0 to 100% of buffer B (0.1% trifluoroacetic acid, 80% acetonitrile) over 5 min, while monitoring by absorbance at 280 or 218 nm. The peaks corresponding to peptides P1 or P5 were further analyzed using MALDI-TOF Mass Spectrometry .
Far-UV circular dichroism measurements
Far-UV circular dichroism (CD) was performed using 10 μM of each peptide solution in PBS. Each spectrum was recorded from 260 nm to 190 nm at 25°C by using a Jasco CD spectrophotometer (Jasco, Japan) with a cell path length of 0.05 cm. Spectra were collected as an average of three scans, with a scan speed of 20 nm/min and a response time of 2 sec. The control (solvent) CD spectra were subtracted to eliminate background effects. Data were reported  in millidegrees and converted to molar ellipticity [θ] in units as follows: degree cm2 dmol-1. The percentage of α-helical content was estimated by assuming that a [θ]222 value of -33000 deg cm2 dmol-1corresponds to 100% α-helix.
Construction of viruses carrying primary envelope glycoproteins
We constructed viruses bearing the envelope glycoproteins derived from virus populations present before and during T20 treatment of a patient who received T20 as part of a salvage therapy, as we reported earlier. Hence, a portion of the envelope gene encoding gp120 and the ectodomain of gp41 (nt 6480 to 8263), previously subcloned into the pCR2.1TOPO vector, was amplified with primers that allowed the introduction of two restriction enzyme sites (AgeI at position 6346 and NheI at position 8287) and digested. Each chimeric proviral clone was obtained by replacing the AgeI-NheI fragment of a modified pNL4-3 vector (nt 6346 to 8287 in pNL4-3) by a AgeI-NheI PCR fragment amplified from the envelope subclone. Sequences of oligonucleotides used for PCR and site-directed mutagenesis can be provided upon request. The pre-T20 virus expressing envelope glycoproteins derived from the pretherapy virus population did not carry the well characterized T20-resistance mutations, which are for the most part located between residues 36 to 45 of the NHR region of gp41 [Sista, 2002 #47] . The envelope proteins of the two other viruses, T20-1 and T20-2, as well as the virus populations from which they were derived, expressed the V38A resistance mutation .
Cells and virus stocks
Hela-CD4-LTR-LacZ cells (P4.2, kindly provided by Dr. M. Alizon, Institut Cochin, Paris, France) stably express human CD4 and CXCR4, as well as the β-galactosidase-encoding gene lacZ under the transcriptional control of the HIV-1 long terminal repeat (LTR), which is activated by HIV-1 Tat . Hela-env-Lai cells stably express HIV-Lai env genes from X4 virus and Tat in the cytosol. All cell lines were cultured in Dulbecco's modified Eagle medium (DMEM, GIBCO) containing physiological concentration of calcium (1.8 mM) supplemented with 10% heat-inactivated fetal calf serum (FCS), 100 U/ml penicillin, 100 mg/ml streptomycin and 2 mM glutamine. The human cell line U373MG-CD4-CCR5-LTR-lacZ, stably expressing the human receptors CD4 and CCR5 and bearing the lacZ indicator cassette , was propagated in DMEM supplemented with 60 μg/ml penicillin, 100 μg/ml streptomycin, and 10% heat-inactivated fetal calf serum. Peripheral blood mononuclear cells (PBMC) were isolated from heparinized human blood by Ficoll (Ficoll-Paque PLUS, Amersham Bioscience) gradient centrifugation and resuspended in fetal calf serum supplemented with 10% DMSO for storage in liquid nitrogen PBMC were activated by incubation in RPMI 1640 medium containing 5 μg/ml phytohemagglutinin-L (PHA-L, Sigma), 100 U/ml penicillin, 100 mg/ml streptomycin, 2 mM glutamine and 10% FCS. After three days of incubation with PHA, cells were washed and used for infection. All cells were cultured in 5% CO2 incubators at 37°C. Stocks of HIV-1 strains JRCSF (R5 virus) and Lai (X4 virus) were produced by transfection of 293 T cells by the calcium phosphate method and stored at -80°C . Chimeric HIV-1 particles expressing the gp120 and gp41 ectodomain of patient origin and the cytoplasmic domain of gp41 from pNL4.3 were produced as previously described . Culture supernatants were collected 40 h after transfection. HIV-1 p24 antigen production was quantified for each viral stock by enzyme-linked immunosorbent assay (Kit p24 Innogen, Innogenetics/Ingen).
HIV-1 env-mediated cell-to-cell fusion assay
Hela-env-Lai cells were seeded in 48-well plates (105 cells per well) in the presence of peptides (P1, P5, P5L, P7, T20) at concentrations ranging from 50 to 1000 nM. Fifteen minutes later, target cells (HeLa P4.2) were added to the wells (105 per well) and co-cultured for 6 h at 37°C. Then, cell-cell fusion was monitored by beta-galactosidase assay . Briefly, cells were lysed in 80 μl of lysis buffer (5% NP40 in PBS without CaCl2 and MgCl2) for 10 min and then centrifuged at 10,000 g for 5 min at 4°C. Equal volumes (50 μl) of lysate and reaction buffer (120 mM Na2HPO4, 80 mM NaH2PO4, 2 mM MgSO4, 20 mM KCl, 10 mM β-mercaptoethanol, 16 mM CPRG (chlorophenol red-β-D-galactopyranoside, Roche, France) were combined and incubated for 60 min at room temperature in the dark. The absorbance at 590 nm of each sample was measured.
Neutralization of HIV-1 infection of PBMC
Neutralization of HIV-1 infectivity for PBMC was performed as previously described  by analysing intracellular staining of p24 Ag after a single round of infection. Briefly, equal volumes (25 μl) of HIV-1Lai or HIV-1JRCSF virus (the amount of virus added per well corresponds to 50 ng of p24) and peptides T20, P1 or P5 at the indicated concentration were incubated together for 1 h at 37°C. PHA-stimulated PBMC (25 μl, 2 × 105 cells/well) were added to the mixture. After overnight incubation, 100 μl of culture medium containing 20 U/ml of IL-2 were added and cells were cultured for an additional 24 h at 37°C. For intracellular p24 Ag staining, cells were fixed with 4% paraformaldehyde for 20 min, permeabilized with 0.05% saponin and stained with a fluorescent anti-p24 mAb (PE-anti-p24, KC57 clone, Beckman Coulter, Hialeah, FL) as a 1/160 dilution in 1% BSA, 0.05% saponin in PBS (pH = 7.4) for 15–20 min at 4°C. The percentage of p24 positive cells was monitored by flow cytometry (FACScalibur with the XL software) by gating 20,000 events on the living cell population identified by forward- and side-scatter parameters.
Inhibition of T20-resistant HIV-1 strains by P5 and T20
Two T20-sensitive (pre- Enfurvitide (ENF) and JRCSF) and two T20-resistant strains (ENF-1 and ENF-2) were assayed for their susceptibility to P5 and T20. These assays were performed as previously described . Two days before infection, U373MG-CD4-CCR5-LTR-lacZ cells were seeded into 96-well plates at a density of 2,000 cells/well. Infection with 10 ng of p24/well of fresh viral suspension was carried out in triplicate, in the absence or the presence of increasing concentrations of T20 (from 1 to 3,3750 nM) (T20; American Peptide Company, Inc), and of P5 (from 1 to 2,000 nM). Forty hours after infection, cells were lysed using 100 μl/well of lysis buffer (5 mM MgCl2 and 0.1% NP-40 in 1× phosphate-buffered saline), prior to addition of 100 μl/well of chromogenic substrate (6 mM chlorophenol red-β-D-galactopyranoside; CPRG, Roche) in lysis buffer. The concentrations of T20 inhibiting virus infectivity by 50% (IC50 values) were calculated by using the median-effect equation .
Binding of Peptides to CD4+-target cells
Binding of peptides was evaluated using either the lymphocytic cell line CD4+- CEM-NKr (NK-resistant) cells (NIH AIDS Reagent Programm, USA)or PHA-activated CD4+ T cells purified from peripheral blood of 5 healthy donors using human CD4+ T cells enrichment kit (StemCell Technologies Inc., France). 5 × 105 cells were incubated with indicated concentrations of peptide for 1 h at room temperature in a total volume of 50 microliter/μl of RPMI 10%FCS. After 2 wahses in cold medium, bound peptide was detected with the human IgG 2F5 (10 microg/μg), or irrelevant human IgG as control, for 1 h at 37°C followed anti-human IgG-FITC mAb (1:1000, Jackson ImmunoResearch, France). Binding of peptides to CD4+ cells was evaluated by flow cytometry (FACSCalibur, Becton Dickinson) on 104 events, gated by forward and side scatter and analyzed for FL1 (FITC channel) using the Cytomix RXP software. Data are presented as peptide-bound cells % total gated cells to which nonspecific background evaluated with the control IgG was deduced (background value less than 10% of the total counts).
In previous studies, we have investigated the structure-function relationship of long synthetic peptides derived from the extracellular domain of gp41. Such long peptides are highly hydrophobic and not easy to produce by chemical synthesis. Furthermore, the role of the transmembrane region of gp41 on the MPR structure and function cannot be analyzed. We therefore chose to express the gp41-derived peptides P1, P5, P5L and P7 in fusion with a cleavable carrier by using a prokaryote expression vector (pGEV) (see Additional file 1).
Secondary structure analysis of gp41-derived peptides by far UV CD spectra
The α-helical content of P1 & P5 alone or in the presence of N36 & LZ
P5, comprising P1 and the gp41 calcium-binding site, inhibits HIV-1 env mediated-cell fusion and infection
Cell fusion experiments are convenient assays for analysis of the mechanism by which peptides inhibit virus entry into host cells. Hela-CD4-LTR-LacZ (P4.2) cells stably express the human CD4 molecule, CXCR4 and long terminal repeat (LTR) driven lacZ gene. Hela-env-Lai cells stably express HIV-Lai envelope glycoproteins gp120 and gp41, and the HIV-1 transactivator Tat. Fusion of Hela-Env-Lai cells and P4.2 cells results in transferring Tat to P4.2 cell cytosol. Tat in turn, transactivates the LTR and initiates transcription of the lacZ gene. The extent of fusion is directly related to β-galactosidase activity in cell lysates .
Inhibition of HIV-1 mediated cell-cell fusion by gp41 MPR derived peptides
(nM, Mean ± S.D.)
(nM, Mean ± S.D.)
913 ± 13.6
P1 + LZ
923.4 ± 11.7
78 ± 5.6
T20 + LZ
579 ± 3.5
60 ± 2.
P5 + LZ
61 ± 1.5
90 ± 1.7
560 ± 10.7
Peptides LZ and N36 at 1 μM exhibit negligible inhibition of cell-cell fusion. The presence of LZ did not substantially modify the efficiency with which P5 inhibited cell-cell fusion, in contrast with T20 (Table 2) and in agreement with the data on the structure of the peptides (Table 1). The antiviral properties of peptides P5, P1 and T20 were tested against infection of PBMC by X4- (HIV-1Lai) and R5-tropic (HIV-1JRCSF) strains of HIV-1. As shown in Figure 3b, and 3c, at 1 μM the P5 and T20 peptides had an inhibitory activity greater than 90% for both X4 and R5 virus infection. IC50 of infection of PBMC by and R5-tropic (HIV-1JRCSF) strains was 20% lower for P5 as compared to T20. Strikingly, P5 mutated in the calcium-binding site (Δ Ca2+ bs-P5) had no antiviral activity, suggesting that the calcium-binding site of P5, absent from T20 is determinant in P5 antiviral activity. In contrast, P1 did not inhibit HIV-1 infection of PBMC under the same conditions.
The mechanisms underlying the anti-HIV-1 activity of T20 remain unclear. It has been proposed that T20 directly interacts with gp41 and gp120, thereby inhibiting viral and cellular membrane fusion [36, 37]. Moreover, the tryptophan-rich region (a.a.666–673) in T20 could interact with the membrane surface, thereby blocking membrane fusion at a post-lipid mixing stage [36, 38]. Although T20 is successfully used in the clinical setting, drug resistant mutants have been reported [19, 29], and in particular in the 3 amino acid GIV motif, located in the NHR of gp41, an important binding site for T20.
Peptides derived from the CHR region of the gp41 ectodomain possess strong anti-HIV activity by virtue of interacting with the coiled-coil motif of gp41 [15, 39]. Peptide P5 derived from this domain comprises three additional non-polar residues, Trp628, Trp631 and Ile635, as compared with T20 or P1, which could increase the binding affinity of the peptide for a hydrophobic cavity in the coiled-coil region. Additionally, the calcium binding site present in P5 but not in T20 and P1, most likely allow the peptide to adopt an inhibitory conformation in physiological conditions where calcium concentration reaches the micromolar range . Moreover, C-peptides containing the hydrophobic cavity-binding region (a.a.628–635) are much less sensitive to the emergence of resistant virus than T20, which lacks this region .
Altogether these data confirm that T20 and P5 exhibit antiviral activities by different mechanism, and that P5 specific activity is calcium dependent.
P5 inhibits replication of T20-resistant HIV-1 strains
Several non-exclusive factors may explain the strong anti-HIV-1 activity of P5 reported here. Amino acids 628 to 663 of peptide P5, containing the calcium-binding site, are essential for binding to the NHR region. It may overcome the interaction with the three non-polar residues GIV that uses T20 for direct interaction with viral gp41 NHR, thereby disrupting the six-helix bundle formation. Furthermore, it would explain how P5 exhibited an antiviral activity against the T20-resistant viruses ENF-1 and -2 that lack this GIV motif. P5 also comprises a lipid-binding domain (666–673) essential for maintaining the anti-HIV-1 activity of T20 [17, 36]. In this regard P5 would be similar to other peptides from the C-terminal heptad repeat that are thought to act as strong anti-HIV-1 activity by binding directly to the virus, thereby preventing hairpin formation and subsequent inhibition of fusion between viral and cellular membranes. Moreover, since P5 harbors a calcium-binding site , in the presence of extracellular calcium P5 might adopt a different conformation due to the saturation of the calcium binding site. Indeed, the P5 peptide, shown to be able to bind directly to the target cell, whereas the mutated P5 did not bind, could compete with the incoming viral particle and thereby inhibit fusion. Accordingly, as shown on Fig. 3c, a P5 peptide with mutation in the amino acids forming the calcium-binding site  exhibited minimal inhibition of CD4+ T cell infection by the R5-tropic JR-CSF virus as compared with wild type P5 (inhibition at a peptide concentration of 250 nM was negligible for mutated P5 (8%) as compared to 65% for wild type P5). This suggests that the superior antiviral activity of P5, as compared with T20, could be attributable to the calcium-binding site conformation along with the tryptophan-rich hydrophobic region immediately adjacent to the viral membrane. These structural elements could confer an efficient antiviral activity, and raise the possibility that calcium plays a role in peptide conformation and its direct interaction with the membrane, either of the virus or the target cell.
In contrast, peptide P1 contains only a partial CHR region without the calcium-binding site and the hydrophobic cavity-binding domain and does not exhibit any anti-HIV-1 activity. The low antiviral activities of P7 peptide indicated that the C-terminal cytoplasmic tail in P7 did not contribute to the antiviral activities. Previous research suggested that C-peptides that had a low tendency to adopt a helical conformation failed to bind to the coiled-coil motif and in turn did not prevent gp41-mediated cell fusion [40, 41].
Accordingly, the CD analysis indicated that peptides P1 and P7 with low anti-HIV-1 activity exhibited lower helical content compared with peptides P5 and P5L with high antiviral activity. The high helical content and the three non-polar residues facing the hydrophobic cavity, together with the structure of the calcium-binding site, are therefore likely to be responsible for the high anti-HIV activity of peptides P5.
In the present study, we found that the antiviral activities of recombinant peptides P1, P5 and P5L were similar to those of their chemically synthesized counterparts (not shown), that pave the way for an easier development of the use of long peptides, usually difficult and costly to produce, as antivirals. Thus recombinant peptides hold promise for HIV-1 salvage treatment in resource-poor settings. Moreover, the expression of recombinant peptides P1, P5 and P5L should enable structural analysis by NMR under different conditions. In particular, study by NMR of isotopically labeled recombinant P1 and P5 in the presence of liposomes of different lipid composition  and of neutralizing antibodies should provide structural information of use in HIV-1 vaccine design. However, generation of escape mutants have restricted the use of T20; and that could apply to other currently approved anti HIV medications. Furthermore, the use of peptides as therapeutics could also be restricted by immunogenicity and half-life in circulation. Hence all these parameters need to be met before a peptide can be a therapeutic.
We want to thank Prs. M. Ghomi and P.Y. Turpin for welcoming us in their laboratory (BIOMOCETI, Evry) and allowing us to use of the Jasco CD apparatus, Pr. B. Desmazieres and Dr. Alain for HPLC and Mass Spectrometry analysis, and Dr. J. Richardson for English editing. We thank Pr P. A. Curmi for providing the pGEV vector and helping us design recombinant peptides. H.Y. and M.B. were supported by fellowships and grants from ANRS and SIDACTION and FRM.
- Bomsel M: Transcytosis of infectious human immunodeficiency virus across a tight human epithelial cell line barrier. Nat Med. 1997, 3 (1): 42-7. 10.1038/nm0197-42.View ArticlePubMedGoogle Scholar
- Alfsen A, Bomsel M: HIV-1 gp41 envelope residues 650–685 exposed on native virus act as a lectin to bind epithelial cell galactosyl ceramide. J Biol Chem. 2002, 277 (28): 25649-59. 10.1074/jbc.M200554200.View ArticlePubMedGoogle Scholar
- Fantini J, Hammache D, Delezay O, Yahi N, Andre-Barres C, Rico-Lattes I, Lattes A: Synthetic soluble analogs of galactosylceramide (GalCer) bind to the V3 domain of HIV-1 gp120 and inhibit HIV-1-induced fusion and entry. J Biol Chem. 1997, 272 (11): 7245-52. 10.1074/jbc.272.11.7245.View ArticlePubMedGoogle Scholar
- Magerus-Chatinet A, Yu H, Garcia S, Ducloux E, Terris B, Bomsel M: Galactosyl ceramide expressed on dendritic cells can mediate HIV-1 transfer from monocyte derived dendritic cells to autologous T cells. Virology. 2007, 362 (1): 67-74. 10.1016/j.virol.2006.11.035.View ArticlePubMedGoogle Scholar
- Alfsen A, Yu H, Magerus-Chatinet A, Schmitt A, Bomsel M: HIV-1-infected blood mononuclear cells form an integrin- and agrin-dependent viral synapse to induce efficient HIV-1 transcytosis across epithelial cell monolayer. Mol Biol Cell. 2005, 16 (9): 4267-79. 10.1091/mbc.E05-03-0192.PubMed CentralView ArticlePubMedGoogle Scholar
- Eckert DM, Kim PS: Mechanisms of viral membrane fusion and its inhibition. Annu Rev Biochem. 2001, 70: 777-810. 10.1146/annurev.biochem.70.1.777.View ArticlePubMedGoogle Scholar
- Chan DC, Kim PS: HIV entry and its inhibition. Cell. 1998, 93 (5): 681-4. 10.1016/S0092-8674(00)81430-0.View ArticlePubMedGoogle Scholar
- Munoz-Barroso I, Salzwedel K, Hunter E, Blumenthal R: Role of the membrane-proximal domain in the initial stages of human immunodeficiency virus type 1 envelope glycoprotein-mediated membrane fusion. J Virol. 1999, 73 (7): 6089-92.PubMed CentralPubMedGoogle Scholar
- Salzwedel K, West JT, Hunter E: A conserved tryptophan-rich motif in the membrane-proximal region of the human immunodeficiency virus type 1 gp41 ectodomain is important for Env-mediated fusion and virus infectivity. J Virol. 1999, 73 (3): 2469-80.PubMed CentralPubMedGoogle Scholar
- Furuta RA, Wild CT, Weng Y, Weiss CD: Capture of an early fusion-active conformation of HIV-1 gp41. Nat Struct Biol. 1998, 5 (4): 276-9. 10.1038/nsb0498-276.View ArticlePubMedGoogle Scholar
- Muster T, Steindl F, Purtscher M, Trkola A, Klima A, Himmler G, Ruker F, Katinger H: A conserved neutralizing epitope on gp41 of human immunodeficiency virus type 1. J Virol. 1993, 67 (11): 6642-7.PubMed CentralPubMedGoogle Scholar
- Stiegler G, Kunert R, Purtscher M, Wolbank S, Voglauer R, Steindl F, Katinger H: A potent cross-clade neutralizing human monoclonal antibody against a novel epitope on gp41 of human immunodeficiency virus type 1. AIDS Res Hum Retroviruses. 2001, 17 (18): 1757-65. 10.1089/08892220152741450.View ArticlePubMedGoogle Scholar
- Zwick MB, Labrijn AF, Wang M, Spenlehauer C, Saphire EO, Binley JM, Moore JP, Stiegler G, Katinger H, Burton DR, Parren PW: Broadly neutralizing antibodies targeted to the membrane-proximal external region of human immunodeficiency virus type 1 glycoproteingp41. J Virol. 2001, 75 (22): 10892-905. 10.1128/JVI.75.22.10892-10905.2001.PubMed CentralView ArticlePubMedGoogle Scholar
- Akari H, Fukumori T, Adachi A: Cell-dependent requirement of human immunodeficiency virus type 1 gp41 cytoplasmic tail for Env incorporation into virions. J Virol. 2000, 74 (10): 4891-3. 10.1128/JVI.74.10.4891-4893.2000.PubMed CentralView ArticlePubMedGoogle Scholar
- Debnath AK: Progress in identifying peptides and small-molecule inhibitors targeted to gp41 of HIV-1. Expert Opin Investig Drugs. 2006, 15 (5): 465-78. 10.1517/135437184.108.40.2065.View ArticlePubMedGoogle Scholar
- Jiang S, Lin K, Strick N, Neurath AR: HIV-1 inhibition by a peptide. Nature. 1993, 365 (6442): 113-10.1038/365113a0.View ArticlePubMedGoogle Scholar
- Wild CT, Shugars DC, Greenwell TK, McDanal CB, Matthews TJ: Peptides corresponding to a predictive alpha-helical domain of human immunodeficiency virus type 1 gp41 are potent inhibitors of virus infection. Proc Natl Acad Sci USA. 1994, 91 (21): 9770-4. 10.1073/pnas.91.21.9770.PubMed CentralView ArticlePubMedGoogle Scholar
- Matthews T, Salgo M, Greenberg M, Chung J, DeMasi R, Bolognesi D: Enfuvirtide: the first therapy to inhibit the entry of HIV-1 into host CD4 lymphocytes. Nat Rev Drug Discov. 2004, 3 (3): 215-25. 10.1038/nrd1331.View ArticlePubMedGoogle Scholar
- Lu J, Deeks SG, Hoh R, Beatty G, Kuritzkes BA, Martin JN, Kuritzkes DR: Rapid emergence of enfuvirtide resistance in HIV-1-infected patients: results of a clonal analysis. J Acquir Immune Defic Syndr. 2006, 43 (1): 60-4. 10.1097/01.qai.0000234083.34161.55.View ArticlePubMedGoogle Scholar
- Yu H, Alfsen A, Tudor D, Bomsel M: The binding of HIV-1 gp41 membrane proximal domain to its mucosal receptor, galactosyl ceramide, is structure-dependent. Cell Calcium. 2008, 43: 73-82. 10.1016/j.ceca.2007.04.011.View ArticlePubMedGoogle Scholar
- Ebenbichler CF, Stoiber H, Schneider R, Patsch JR, Dierich MP: The human immunodeficiency virus type 1 transmembrane gp41 protein is a calcium-binding protein and interacts with the putative second-receptor molecules in a calcium-dependent manner. J Virol. 1996, 70 (3): 1723-8.PubMed CentralPubMedGoogle Scholar
- Veazey RS, Klasse PJ, Schader SM, Hu Q, Ketas TJ, Lu M, Marx PA, Dufour J, Colonno RJ, Shattock RJ, Springer MS, Moore JP: Protection of macaques from vaginal SHIV challenge by vaginally delivered inhibitors of virus-cell fusion. Nature. 2005, 438 (7064): 99-102. 10.1038/nature04055.View ArticlePubMedGoogle Scholar
- Hovanessian AG, Briand JP, Said EA, Svab J, Ferris S, Dali H, Muller S, Desgranges C, Krust B: The caveolin-1 binding domain of HIV-1 glycoprotein gp41 is an efficient B cell epitope vaccine candidate against virus infection. Immunity. 2004, 21 (5): 617-27. 10.1016/j.immuni.2004.08.015.View ArticlePubMedGoogle Scholar
- Hollier MJ, Dimmock NJ: The C-terminal tail of the gp41 transmembrane envelope glycoprotein of HIV-1 clades A, B, C, and D may exist in two conformations: an analysis of sequence, structure, and function. Virology. 2005, 337 (2): 284-96. 10.1016/j.virol.2005.04.015.View ArticlePubMedGoogle Scholar
- Kennedy RC, Henkel RD, Pauletti D, Allan JS, Lee TH, Essex M, Dreesman GR: Antiserum to a synthetic peptide recognizes the HTLV-III envelope glycoprotein. Science. 1986, 231 (4745): 1556-9. 10.1126/science.3006246.View ArticlePubMedGoogle Scholar
- Gronenborn AM, Filpula DR, Essig NZ, Achari A, Whitlow M, Wingfield PT, Clore GM: A novel, highly stable fold of the immunoglobulin binding domain of streptococcal protein G. Science. 1991, 253 (5020): 657-61. 10.1126/science.1871600.View ArticlePubMedGoogle Scholar
- Redeker V, Lachkar S, Siavoshian S, Charbaut E, Rossier J, Sobel A, Curmi PA: Probing the native structure of stathmin and its interaction domains with tubulin. Combined use of limited proteolysis, size exclusion chromatography, and mass spectrometry. J Biol Chem. 2000, 275 (10): 6841-9. 10.1074/jbc.275.10.6841.View ArticlePubMedGoogle Scholar
- Chen YH, Yang JT, Chau KH: Determination of the helix and beta form of proteins in aqueous solution by circular dichroism. Biochemistry. 1974, 13 (16): 3350-9. 10.1021/bi00713a027.View ArticlePubMedGoogle Scholar
- Rimsky LT, Shugars DC, Matthews TJ: Determinants of human immunodeficiency virus type 1 resistance to gp41-derived inhibitory peptides. J Virol. 1998, 72 (2): 986-93.PubMed CentralPubMedGoogle Scholar
- Labrosse B, Morand-Joubert L, Goubard A, Rochas S, Labernardiere JL, Pacanowski J, Meynard JL, Hance AJ, Clavel F, Mammano F: Role of the envelope genetic context in the development of enfuvirtide resistance in human immunodeficiency virus type 1-infected patients. J Virol. 2006, 80 (17): 8807-19. 10.1128/JVI.02706-05.PubMed CentralView ArticlePubMedGoogle Scholar
- Dragic T, Alizon M: Different requirements for membrane fusion mediated by the envelopes of human immunodeficiency virus types 1 and 2. J Virol. 1993, 67 (4): 2355-9.PubMed CentralPubMedGoogle Scholar
- Labrosse B, Brelot A, Heveker N, Sol N, Schols D, De Clercq E, Alizon M: Determinants for sensitivity of human immunodeficiency virus coreceptor CXCR4 to the bicyclam AMD3100. J Virol. 1998, 72 (8): 6381-8.PubMed CentralPubMedGoogle Scholar
- Nussbaum O, Broder CC, Berger EA: Fusogenic mechanisms of enveloped-virus glycoproteins analyzed by a novel recombinant vaccinia virus-based assay quantitating cell fusion-dependent reporter gene activation. J Virol. 1994, 68 (9): 5411-22.PubMed CentralPubMedGoogle Scholar
- Mascola JR, Louder MK, Winter C, Prabhakara R, De Rosa SC, Douek DC, Hill BJ, Gabuzda D, Roederer M: Human immunodeficiency virus type 1 neutralization measured by flow cytometric quantitation of single-round infection of primary human T cells. J Virol. 2002, 76 (10): 4810-21. 10.1128/JVI.76.10.4810-4821.2002.PubMed CentralView ArticlePubMedGoogle Scholar
- Chou TC: The median-effect principle and the combination index for quantitation of synergism and antagonism. Synergism and antagonism in chemotherapy. Edited by: Chou TC, Rideout DC. 1991, Academic Press, San Diego, Calif, 61-102.Google Scholar
- Liu S, Jing W, Cheung B, Lu H, Sun J, Yan X, Niu J, Farmar J, Wu S, Jiang S: HIV gp41 C-terminal heptad repeat contains multifunctional domains. Relation to mechanisms of action of anti-HIV peptides. J Biol Chem. 2007, 282 (13): 9612-20. 10.1074/jbc.M609148200.View ArticlePubMedGoogle Scholar
- Liu S, Lu H, Niu J, Xu Y, Wu S, Jiang S: Different from the HIV fusion inhibitor C34, the anti-HIV drug Fuzeon (T-20) inhibits HIV-1 entry by targeting multiple sites in gp41 and gp120. J Biol Chem. 2005, 280 (12): 11259-73. 10.1074/jbc.M411141200.View ArticlePubMedGoogle Scholar
- Kliger Y, Gallo SA, Peisajovich SG, Munoz-Barroso I, Avkin S, Blumenthal R, Shai Y: Mode of action of an antiviral peptide from HIV-1. Inhibition at a post-lipid mixing stage. J Biol Chem. 2001, 276 (2): 1391-7. 10.1074/jbc.M004113200.View ArticlePubMedGoogle Scholar
- Chan DC, Chutkowski CT, Kim PS: Evidence that a prominent cavity in the coiled coil of HIV type 1 gp41 is an attractive drug target. Proc Natl Acad Sci USA. 1998, 95 (26): 15613-7. 10.1073/pnas.95.26.15613.PubMed CentralView ArticlePubMedGoogle Scholar
- Jin BS, Ryu JR, Ahn K, Yu YG: Design of a peptide inhibitor that blocks the cell fusion mediated by glycoprotein 41 of human immunodeficiency virus type 1. AIDS Res Hum Retroviruses. 2000, 16 (17): 1797-804. 10.1089/08892220050195757.View ArticlePubMedGoogle Scholar
- Judice JK, Tom JY, Huang W, Wrin T, Vennari J, Petropoulos CJ, McDowell RS: Inhibition of HIV type 1 infectivity by constrained alpha-helical peptides: implications for the viral fusion mechanism. Proc Natl Acad Sci USA. 1997, 94 (25): 13426-30. 10.1073/pnas.94.25.13426.PubMed CentralView ArticlePubMedGoogle Scholar
- Coutant J*, Yu H*, Alfsen A, Toma F, Curmi P, Bomsel M: Both lipid environment and pH are critical for determining physiologic solution structure of 3D- conserved epitopes of the HIV-1 gp41-MPER peptide P1. Faseb J. 2008, in press.Google Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.