Figure 4

Binding of Peptides to CD4⁺-target cells. P5, P5 mutated in the calcium-binding site (P5m), and T20 at indicated concentrations were allowed to interact the CD4⁺-target cells: primary human CD4⁺ T cells or the lymphocytic CD4⁺- CEM cell line. Bound peptide were detected using the IgG 2F5 and analyzed by flow cytometry. Results are shown in a two-scale bar graph (human CD4⁺ T cells (left scale), CD4⁺- CEM cell line (right scale)) as peptide bound cells % the gated population, to which non specific binding has been subtracted.