Microbial exposure alters HIV-1-induced mucosal CD4+ T cell death pathways Ex vivo
Retrovirologyvolume 11, Article number: 14 (2014)
Early HIV-1 infection causes massive CD4+ T cell death in the gut and translocation of bacteria into the circulation. However, the programmed cell death (PCD) pathways used by HIV-1 to kill CD4+ T cells in the gut, and the impact of microbial exposure on T cell loss, remain unclear. Understanding mucosal HIV-1 triggered PCD could be advanced by an ex vivo system involving lamina propria mononuclear cells (LPMCs). We therefore modeled the interactions of gut LPMCs, CCR5-tropic HIV-1 and a commensal gut bacterial species, Escherichia coli. In this Lamina Propria Aggregate Culture (LPAC) model, LPMCs were infected with HIV-1BaL by spinoculation and cultured in the presence or absence of heat killed E.coli. CD4+ T cell numbers derived from flow cytometry and viable cell counts were reported relative to mock infection. Viable cells were identified by viability dye exclusion (AqVi), and intracellular HIV-1 Gag p24 protein was used to identify infected cells. Annexin V and AqVi were used to identify apoptotic versus necrotic cells. Caspase-1 and Caspase-3 activities were blocked using specific inhibitors YVAD and DEVD, respectively.
CD4+ T cell depletion following HIV-1 infection was reproducibly observed by 6 days post infection (dpi). Depletion at 6 dpi strongly correlated with infection frequency at 4 dpi, was significantly blocked by Efavirenz treatment, and was primarily driven by p24-negative cells that were predominantly necrotic. HIV-1 infection significantly induced CD4+ T-cell intrinsic Caspase-1 activity, whereas Caspase-1 inhibition, but not Caspase-3 inhibition, significantly blocked CD4+ T cell depletion. Exposure to E.coli enhanced HIV-1 infection and CD4+ T depletion, and significantly increased the number of apoptotic p24+ cells. Notably, CD4+ T cell depletion in the presence of E.coli was partially blocked by Caspase-3, but not by Caspase-1 inhibition.
In the LPAC model, HIV-1 induced Caspase-1 mediated pyroptosis in bystander CD4+ T cells, but microbial exposure shifted the PCD mechanism toward apoptosis of productively infected T cells. These results suggest that mucosal CD4+ T cell death pathways may be altered in HIV-infected individuals after gut barrier function is compromised, with potential consequences for mucosal inflammation, viral dissemination and systemic immune activation.
HIV-1 infection is characterized by high levels of virus replication, gradual peripheral CD4+ T cell depletion, and aberrantly high immune activation. Chronic immune activation may result from pathogenic events in the gut mucosa established during early infection, when viral replication in the intestinal mucosa results in massive killing of lamina propria (LP) CD4+ T cells, enteropathy, inflammation, and microbial translocation [1, 2]. However, the mechanisms and pathways involved in HIV-associated LP CD4+ T cell depletion remain key unanswered questions in basic HIV research [3, 4].
Major efforts to investigate the mechanisms of HIV-1 mediated CD4+ T cell depletion have been made using ex vivo models of HIV-1 infection in primary human CD4+ T cells or cell lines. Ex vivo modeling studies of HIV-1 infection of primary human CD4+ T cells indicated that HIV-1-mediated killing could occur in both productively-infected and bystander, or nonproductively-infected, cells. CXCR4-tropic (X4) HIV-1 was found to kill resting spleen and tonsil CD4+ T cells ex vivo through abortive infection , whereas double-stranded DNA breaks occurring during HIV-1 integration were responsible for the death of productively-infected CD4+ T cells from peripheral blood . However, it remains unclear whether the death of productively-infected or bystander cells is primarily responsible for driving human LP CD4+ T cell depletion. Interestingly, earlier studies in the SIV/rhesus macaque model also suggested that LP CD4+ T cell death could occur in both productively infected  and bystander  cells to drive depletion. Unraveling the mechanisms underlying HIV-1 mediated LP CD4+ T cell depletion may require the use of primary human LP CD4+ T cell lymphocytes.
Unlike peripheral blood or lymphoid CD4+ T cells, LP CD4+ T cells are predominantly of a recently activated, CCR5hi effector memory phenotype . These cells are highly susceptible to infection by CCR5-tropic HIV-1 strains, which are found in over 90% of chronically HIV-infected patients, and account for most transmitted viruses [10, 11]. The LP CD4+ T cell pool in the gut-associated lymphoid tissue (GALT) is a heterogeneous population comprised of multiple T helper (Th) subsets that have diverse functions in host defense . In particular, the loss of mucosal IL-17 producing T cells (Th17), which play a role in defense against extracellular pathogens, has been closely linked to pathogenic SIV and HIV infection [13–15]. The gut microbiome also plays an important role in establishing the LP microenvironment. In HIV-1 infection, translocation of microbial products strongly correlates with increased immune activation [16–18]. In fact, commensal Gram-negative Escherichia coli (E.coli) was detected in the LP of rhesus macaques during early stages of pathogenic SIV infection . Thus, adequately modeling HIV interactions in the LP must account for any effects of commensal bacteria. We previously showed that commensal E.coli activates resident LP CD4+ T cells in an MHC Class II-dependent fashion , and increases HIV-1 replication in human LP CD4+ T cells ex vivo. However, the impact of microbial exposure on the magnitude and mechanisms of LP CD4+ T cell death remains unknown.
To date, very limited information exists as to which cell death pathway(s) are triggered in human LP CD4+ T cells by R5-tropic HIV-1 infection. Most programmed cell death (PCD) pathways are dependent on proteolytic enzymes known as caspases. In the canonical PCD pathway, apoptosis, cells undergo cell shrinkage, blebbing and DNA fragmentation but retain plasma membrane integrity . Apoptosis can be triggered by either extrinsic (e.g. Fas/FasL) or intrinsic stimuli, but both pathways converge on the effector molecule Caspase-3 . Apoptotic cells are generally disposed of in vivo in a non-inflammatory manner through the exposure of phosphotidylserine (PS) from the inner leaflet of the plasma membrane to the cell surface [24–26]. There is a substantial body of literature suggesting that apoptosis is aberrantly triggered or has become dysregulated during HIV-1 infection [27–29]. In contrast to apoptosis, pyroptosis is a highly inflammatory form of PCD that involves oncosis, plasma membrane rupture, and the rapid release of cytoplasmic contents into the surrounding environment [22, 30]. Pyroptosis has been linked to the ‘inflammasome’, a multimeric complex containing active Caspase-1 and pattern recognition receptors such as NLRP3 [30, 31]. In addition to mediating pyroptosis, Caspase-1 processes pro-IL-1β to the mature secreted form that could contribute to inflammation and epithelial barrier dysfunction [32, 33]. Interestingly, increased Caspase-1 activity has been documented in HIV infection ex vivo in the Human Lymphoid Aggregate Culture (HLAC) model and in primary peripheral blood T cells from HIV infected patients [5, 34, 35]. It remains unknown whether Caspase-1 plays a role in HIV-1 mediated LP CD4+ T cell death.
In this report, we used the Lamina Propria Aggregate Culture (LPAC) model to identify the PCD pathway(s) triggered in primary LP CD4+ T cells ex vivo by infection with an R5 tropic HIV-1 strain. We further assessed the impact of commensal E. coli exposure on the magnitude and mechanisms of HIV-mediated LP CD4+ T cell depletion. We provide evidence for augmented HIV-1 mediated LP CD4+ T cell death and a shift in the PCD pathway following microbial exposure.
CD4+ T cell depletion strongly correlates with productive infection in the LPAC model
To monitor HIV-1 mediated LP CD4+ T cell death, we utilized primary LP mononuclear cells (LPMC) in an experimental strategy which involved quantifying absolute LP CD4+ T cell numbers in HIV-1-infected relative to mock infected cultures . Given the similarities of our experimental approach to that of the HLAC model, we refer to this infection platform as the ‘Lamina Propria Aggregate Culture’ or LPAC model. LPMCs were infected with R5-tropic HIV-1Ba-L by spinoculation and analyzed at the indicated days post-infection (dpi) (Figure 1A). Since HIV-1 downregulates surface CD4 expression in vitro, CD4+ T cells were identified as CD3+CD8-. Viable CD3+CD8- T cells were identified using the gating strategy shown (Figure 1B). At 4 dpi, the absolute numbers of CD4+ T cells in mock infected and HIV-infected wells were not significantly different (Figure 1C left panel). However, by 6 dpi, HIV-1-infected wells had significantly fewer CD4+ T cells than mock (Figure 1C right panel). To compare the extent of HIV-1-mediated depletion between donors, the survival of CD3+CD8- T cells was normalized to mock (100% survival). Depletion was not statistically significant overall at 4 dpi, but about half (9 of 17) of the samples exhibited some level of depletion (‘depleters’; Figure 1D). Depletion was significantly greater at 6 dpi than at 4 dpi (Figure 1D). CD3+CD8+ T cells and CD3-CD19+ B cells were not depleted relative to mock infection (Figure 1E). Thus, LP CD4+ T cell depletion can be specifically and reliably quantified in the LPAC model by 6 dpi.
We then assessed the relationship between productive HIV-1 infection and LP CD4+ T cell depletion. Productive HIV-1 infection was measured using intracellular HIV-1 Gag-p24 (p24) expression. Positive p24 expression was established from the mock infected control (<1% p24+) (Figure 2A). The frequency of CD3+CD8-p24+ cells at 4 dpi strongly correlated with LP CD4+ T cell depletion at 6 dpi in HIV-infected cultures (Figure 2B). In addition, the percentage of p24+ cells was 2.3-fold greater in the 9 ‘depleter’ samples (Figure 1D) than the 8 ‘non-depleter’ samples (Figure 2C) despite similar cellular LPMC composition prior to infection (Additional file 1: Figure S1). To confirm that depletion was dependent on productive HIV-1 infection, early reverse transcription was inhibited using the antiretroviral drug, Efavirenz. Efavirenz at 1 nM or 10 nM blocked p24 expression and T cell depletion in a dose dependent fashion (Figure 2D). The 10 nM Efavirenz dose was sufficient to significantly decrease p24 expression and increase the survival of CD3+CD8- T cells relative to untreated HIV infection (Figure 2E). These results indicate that in the LPAC model, active HIV-1 replication is required for LP CD4+ T cell depletion.
HIV-1 infection increases the frequency of CD4 T cells with apoptotic and necrotic phenotypes
To address potential mechanisms of HIV-1-mediated LP CD4+ T cell death, we profiled the CD4+ T cells either as early apoptotic (AnnexinV+AqVi-) or necrotic (AnnexinV+AqVi+) by flow cytometry (Figure 3A). Multiple PCD pathways, including apoptosis that has proceeded to secondary necrosis, pyroptosis, and necroptosis, converge on the necrotic phenotype given sufficient time [24, 25, 37]. Cell death profiles were measured at 4 dpi when early apoptotic cells were detectable. HIV-1 infection increased the percentage of CD4+ T cells expressing both apoptotic and necrotic phenotypes relative to mock (Figure 3B).
We next compared the p24+ and p24neg populations to gain insight into their respective contributions to total CD4+ T cell loss. The percentage of cells with an apoptotic phenotype was greater in the p24+ population, whereas the percentage of cells with a necrotic phenotype was greater in p24neg population (Additional file 2: Figure S2A). However, since there were more p24neg cells than p24+ cells in the culture, the absolute numbers of apoptotic p24+ and p24neg cells were similar at 4 dpi (Figure 3C), suggesting that apoptosis contributed equally to depletion in both subsets. In sharp contrast, the absolute number of p24neg cells with a necrotic phenotype was 34-fold greater than the number of necrotic p24+ cells (Figure 3C). Presented another way, 63% of the CD4+ T cells committed to dying (AnnexinV+) were p24neg and had a necrotic phenotype (Figure 3D), indicating that necrotic cell death in the p24neg population accounted for the majority of depletion during HIV-1 infection. The p24+ cells accounted for only 13% of the total dying cells (Figure 3D), but it was the magnitude of p24+ cell death that correlated with HIV-1 mediated LP CD4+ T cell depletion measured at 6 dpi (compare Figure 3E and F).
HIV-1 promotes LP CD4+ T cell death predominantly through Caspase-1-mediated pyroptosis
We next considered non-apoptotic PCD pathways that could lead to a necrotic phenotype. Pyroptosis is a Caspase-1-dependent PCD pathway that was recently implicated in HIV-1-mediated death of resting lymphoid T cells . Caspase-1 activity was measured by flow cytometry in mock or HIV-infected LPMCs at 2 and 6 dpi using a fluorogenic Caspase-1 substrate (CaspaLux-1). CD3+CD8- T cells with active Caspase-1 were identified from a tight lymphocyte gate (Figure 4A-B). Caspase-1 activity was significantly induced in LP CD4+ T cells in HIV-infected cultures compared to mock by 6 dpi (Figure 4C). These findings provide evidence for LP CD4+ T cell intrinsic Caspase-1 activity that occurred concurrent with depletion.
Irreversible peptide inhibitors were used to block the function of Caspase-1 (pyroptosis; YVAD) and Caspase-3 (apoptosis; DEVD) to determine their relative effects on HIV-1 induced depletion. To avoid toxicity, we used the highest possible dose (25 μM) of each inhibitor at a non-toxic DMSO concentration and added each inhibitor (25 μM) at days 0 and 2 (Figure 1A). Depletion was determined relative to mock infection and normalized to the DMSO vehicle control (100% depletion) to determine the percent protection from depletion with each inhibitor for each donor (Figure 4D; representative donor). Neither DEVD nor YVAD significantly altered the frequency of p24+ CD4+ T cells compared to the DMSO control, indicating that HIV-1 replication was not impeded by the presence of drug (Additional file 3: Figure S3A). In 6 donors, HIV-1 infection significantly depleted LP CD4+ T cells by 4 dpi relative to mock infection with DMSO (Figure 4E). DEVD did not significantly inhibit LP CD4+ T cell depletion (Figure 4E). However, YVAD significantly attenuated HIV-1 mediated depletion from 36% to 18% (Figure 4E) indicating a role for Caspase-1 activity in HIV-1 mediated LP CD4+ T cell death.
Exposure to commensal E. colialters the kinetics and increases the magnitude of HIV-1 mediated LP CD4+ T cell depletion in the LPAC model
We previously reported that commensal E. coli exposure augmented HIV-1 infection of LPMCs , but the impact of E. coli exposure on LP CD4+ T cell death remained unknown. As expected, E. coli exposure in the LPAC model significantly increased the frequency (Figure 5A) and number (data not shown) of p24+ cells at 4 dpi and 6 dpi relative to HIV-1 infection only. Importantly, LP CD4+ T cell depletion was also significantly enhanced at both 4 dpi (Figure 5B left panel) and 6 dpi (Figure 5B right panel) in the presence of E. coli. This contrasts with the kinetics of depletion in the HIV-1 infection without E. coli condition, when reproducible depletion was not observed until 6 dpi (Figure 1D). Of note, no depletion was observed in CD3+CD8+ or CD3-CD19+ cells in the presence of E. coli (Figure 5C), indicating that the enhanced depletion was specific to CD4+ T cells and not due to potential non-specific cell death from direct microbial activation. In summary, exposure to commensal E. coli increased the magnitude and kinetics of LP CD4+ T cell depletion in the LPAC model.
Pathogenic lentivirus infection is associated with extensive depletion of the gut Th17 subset which plays a key role in bacterial defense [13, 14, 38–40], raising the possibility that the Th subsets in the LP may have different susceptibilities to HIV-1 mediated killing. We therefore investigated the susceptibility of Th1 (IFN-γ+IL-17-), Th17 (IL-17+IFN-γ-), Th1/17 (IFN-γ+ IL-17+) and non-Th1/17 (IFN-γ-IL-17-, double negative, DN) subsets to undergo HIV-1 mediated death at 6 dpi in the presence or absence of bacteria. The Th subsets were identified by intracellular cytokine staining by flow cytometry following PMA/Ionomycin stimulation. At 6 dpi, R5-tropic HIV-1 depleted Th1, Th17 and Th1/17 cells in the LPAC model to a similar extent (Figure 5D). In contrast, DN cells were relatively resistant to HIV-1 mediated killing (Figure 5D). Commensal E. coli exposure led to 2 to 3-fold increased LP T cell depletion in the Th1, Th17 and DN subsets compared to HIV-1 alone (Figure 5D). Remarkably, E. coli enhanced depletion in the Th1/17 depletion by 10-fold (Figure 5D). As with HIV-1 infection alone (Figure 2B), the level of productive infection was strongly associated with the level of depletion following E. coli exposure (Figure 5E; Spearman r for samples with E.coli = 0.5760; p = 0.017).
Exposure to E. coliincreases the death of productively infected cells through increased apoptosis
Since E. coli exposure increased the number of p24+ cells and overall depletion, we assessed the proportion of cells with an early apoptotic or necrotic phenotype in the p24+ and p24neg populations (Figure 3A) in the presence of E. coli. As with HIV-1 infection alone, p24+ cells remained more likely to have an apoptotic phenotype than p24neg cells, whereas p24neg cells remained more likely to have a necrotic profile than p24+ cells (Additional file 2: Figure S2B). Notably, the absolute number of p24+ cells with both apoptotic and necrotic phenotypes increased significantly in the presence of E. coli compared to HIV-1 alone (Figure 6A). In contrast, E. coli had no effect on the absolute number of p24neg cells with either an apoptotic or necrotic phenotype (Figure 6B). In total, p24+ cells accounted for 39% of the CD4+ T cell death in the presence of E. coli (Figure 6C), in sharp contrast to HIV-1 infection only (Figure 3D). Importantly, the majority of the p24+ CD4+ T cells were apoptotic in the presence of E. coli. The addition of E. coli significantly increased the percentage of cells with an apoptotic phenotype by 1.5-fold in mock infected cells (Figure 6D) but did not alter the percentage of cells with a necrotic phenotype (Figure 6D). Thus, increased HIV-1 mediated LP CD4+ T cell apoptosis in the presence of E.coli likely reflects both virus- and bacteria-induced cell death.
DEVD and YVAD were used to determine whether Caspase inhibition could block HIV-1 associated LP CD4+ T cell death in the presence of E. coli. The inhibitors did not significantly decrease the LP CD4+ T cell number in the mock plus E. coli condition (Additional file 3: Figure S3C-D). LP CD4+ T cell depletion in the presence of HIV-1 plus E. coli was significantly blocked by DEVD compared to the DMSO control (Figure 6E). Surprisingly, Caspase-1 inhibition by YVAD did not significantly prevent LP CD4+ T cell depletion with virus and E. coli present (Figure 6E). The results suggest that in the presence of E.coli, HIV-1 mediated LP CD4+ T cell death is Caspase-3, but not Caspase-1, dependent.
Counteracting inflammation and immune activation in HIV-1 infection may require resolving critical knowledge gaps about how HIV-1 causes extensive LP CD4+ T cell death during primary infection. Here, we demonstrate the versatility of the LPAC model in addressing these critical questions regarding the mechanisms underlying R5-tropic HIV-1 mediated mucosal CD4+ T cell death. We provide evidence that HIV-1 induces pyroptosis of non-productively infected LP CD4+ T cells based on the detection of active Caspase-1 in CD4+ T cells and the inhibition of LP CD4+ T cell depletion by blocking Caspase-1 activity. Surprisingly, our results with primarily effector memory LP CD4+ T cells mirrored published findings based on X4-tropic HIV-1 infection of HLAC cells , which are predominantly resting CD4+ T cells. Thus, despite the important differences in CD4+ T cell phenotypes between the two ex vivo models, pyroptosis appears to be a critical HIV-1-induced PCD pathway in non-productively infected CD4+ T cells.
The molecular trigger(s) for Caspase-1 mediated death in the LPAC model remain unknown, but recent high-profile studies suggest plausible mechanisms [5, 6, 41, 42]. One possibility is that the accumulation of incomplete HIV-1 reverse transcripts in abortively infected LP CD4+ T cells triggered death, analogous to that reported in the HLAC model . In this model, IFI16 has been identified as the DNA sensor that triggers pyroptosis in the bystander T cells , and p24+ cells were shown to be the killing unit rather than the free virus . Notably, in the LPAC model, it was the number of dying p24+, but not p24neg cells, which significantly correlated with depletion. If a similar PCD mechanism operates in the LPAC as in the HLAC model, each p24+ cell could induce cell death in variable number of bystander cells, thereby weakening the association between p24neg cells and depletion. However, LP CD4+ T cells are more susceptible to HIV-1 infection than resting HLAC CD4+ T cells due to higher activation levels and expression of HIV-1 co-receptors [9, 43]. Thus, at this time, we cannot confirm that abortive infection triggers pyroptosis in the gut and cannot exclude alternative triggers for Caspase-1 activity. Nabel and colleagues recently reported decreased HIV-1 gene expression following DNA-PK recognition of double-stranded DNA breaks during integration . Thus, an alternative hypothesis is that HIV-1 gene expression is being quickly shut off in permissive LP CD4+ T cells upon integration induced cell death, and permissive cells dying post-HIV-1 integration could appear as p24neg. Loss of viral protein expression in permissive cells could also potentially explain the strong association we observed between the frequency of productively infected cells and depletion. However, to our knowledge, there is no established link between DNA-PK and Caspase-1 activities.
Caspase-1 activity may be a potential link between HIV-1-mediated LP CD4+ T cell death and HIV-1-mediated mucosal inflammation. In antigen presenting cells, Caspase-1 cleaves pro-IL-18 and pro-IL-1β to their active forms . Increased IL-1β expression was observed in the LP during acute pathogenic SIV, but not during non-pathogenic infection, suggesting that Caspase-1 activity could be a mediator of lentivirus pathogenesis . IL-1β, other pro-inflammatory cytokines, and HIV-1 gp120 have been shown to cause tight junction breakdown between intestinal epithelial cells in vitro[32, 44]. Furthermore, pyroptosis releases cellular contents into the environment that can act as danger-associated molecular patterns (DAMPs), thereby triggering inflammation in the local microenvironment [30, 31]. Thus, Caspase-1 activity in LP CD4+ T cells could precipitate epithelial barrier dysfunction by releasing either traditional inflammatory cytokines or cellular DAMPs.
Epithelial barrier breakdown results in microbial translocation during the later stages of acute SIV infection , suggesting that studies in the LPAC model are incomplete without taking enteric bacteria into account. We previously reported  that exposure to commensal bacteria increased productive infection in LP CD4+ T cells in vitro, likely by enhancing T cell activation. We now link these findings to increased LP CD4+ T cell depletion in the presence of commensal bacteria. In addition, the LPAC data for Th subsets recapitulated the high susceptibility of IL-17-producing cells to HIV-1-mediated depletion observed in vivo and highlighted a role for microbial exposure in exacerbating this process [13, 14, 38–40].
Exposure to E. coli increased overall LP CD4 T cell depletion by increasing the number of productively infected cells dying by apoptosis. The involvement of apoptosis as the dominant PCD pathway in the presence of E. coli was further supported by significant inhibition of HIV-1-mediated depletion by blocking Caspase-3 activity. Several viral proteins such as protease, gp120, Tat, Nef and Vpr have been reported to facilitate apoptotic CD4+ T cell death [27, 28]. Enhanced HIV-1 infection following microbial exposure could lead to increased cellular levels of these viral proteins, thereby triggering apoptosis. Indirectly, microbial products may also sensitize LP CD4+ T cells to undergo apoptosis at a lower signaling threshold. For example, LPS and inflammatory cytokines have been reported to increase tryptophan catabolism by DCs , potentially causing local tryptophan depletion, which then sensitizes activated T cells to apoptosis [45, 46].
Our findings in the ex vivo LPAC model have potential implications for understanding how HIV-1 induces LP CD4+ T cell depletion in vivo. Based on our ex vivo modeling results, we theorize that mucosal pathogenesis may proceed in two phases during early HIV-1 infection: (1) there could be an ‘early phase’ of depletion, before intestinal barrier breakdown occurs, when depletion and inflammation are simultaneously driven by Caspase-1 mediated PCD in uninfected LP CD4+ T cells; and (2) a ‘late phase’ that is driven by the onset of microbial translocation, with exposure to microbial products resulting in increased HIV-1 replication and a larger role for apoptotic death of infected LP CD4+ T cells in ongoing depletion (Figure 7). The potential in vivo consequences of apoptotic death of mucosal T cells could include a weakened adaptive immunity as highlighted by other investigators [47, 48]. Corroborating this ‘biphasic model’ of early HIV-1 mucosal pathogenesis will require directly evaluating tissues from HIV-1 infected patients at varying disease stages. Further experiments in the LPAC model, informed by in vivo studies, should help refine mechanistic models of LP CD4+ T cell depletion and inflammation to ultimately provide a better understanding of HIV-1 mucosal pathogenesis.
The data establish the Lamina Propria Aggregate Culture (LPAC) model as a robust and versatile platform for studying the impact of R5-tropic HIV-1 infection and commensal microbial species on mucosal CD4+ T cell death. Using the LPAC model, we provide evidence for Caspase-1 mediated T cell pyroptosis as a primary mechanism for LP CD4+ T cell death in bystander cells. However, upon exposure to commensal E. coli, LP CD4+ T cell death was augmented and shifted to Caspase-3 mediated apoptosis due to a significant increase in productively-infected cells. The results suggest a biphasic model of LP CD4+ T cell death during acute HIV infection, in which distinct CD4+ T cell death pathways, demarcated by intestinal barrier dysfunction and microbial translocation, may converge to drive mucosal inflammation, viral dissemination and systemic immune activation.
Intestinal tissue samples
Macroscopically normal human jejunum tissue samples, that would otherwise be discarded, were obtained in a de-identified manner from 17 patients undergoing elective abdominal surgery. Those with a history of inflammatory bowel disease, recent chemotherapy, radiation, or other immunosuppressive drugs were excluded from the study. The use of discarded tissue was granted exempt status by the Colorado Multiple Institutional Review Board and patients signed a pre-operative consent form allowing its unrestricted use.
Preparation of LPMCs from intestinal samples
The primary LP cells were obtained using a two-step fractionation process as previously described [20, 21]. EDTA was used to separate the epithelial cells and intraepithelial lymphocytes, followed by Collagenase D (Roche Catalog #: 11088882001) treatment to release the LPMCs. The cells were cryopreserved in RPMI + 10% DMSO + 10% FBS.
Preparation of HIV-1 stocks
MOLT4-CCR5 cells (AIDS Research and Reference Reagent Program; ARRP Catalog# 510) were grown at 0.5 to 1 million (M)/ml in RPMI containing 10% FBS, 1% penicillin/streptomycin/glutamine and 1 mg/ml G418 (propagation media) at 37°C to 50 M total cells. Majority (>90%) of MOLT4-CCR5 cells expressed CD4 and CCR5 by flow cytometry. 25 M MOLT4-CCR5 cells were infected with the R5-tropic HIV-1 Ba-L strain (ARRP Catalog# 4984) with 2 μg/ml polybrene for 2 h. The cells were washed with propagation media and grown at 0.5 to 1 M cells/ml. Supernatants containing virus were collected at 9 dpi and concentrated by ultracentrifugation at 141,000×g for 2 h. The concentration of p24 in the supernatant was determined by HIV Gag p24 ELISA (Perkin Elmer, Walthm, MA). Virus stocks were frozen in single use aliquots at −80°C.
Preparation of heat-killed Eschericia coli
E. coli stocks (ATCC #25922, Manassass, VA) were grown as described previously  and kept at −80°C in single use aliquots.
LPMC infection assay
LPMCs were thawed using a standard protocol. Briefly, 1 ml aliquots of cells were quick thawed at 37°C. The following amounts of thaw media (90 ml RPMI + 10% FBS + 1% penicillin/streptomycin/glutamine + 100 μl DNAse) were added at 1 min intervals: 100 μl, 200 μl, 400 μl, 800 μl, 1.6 ml, 3.2 ml (twice). The cells were centrifuged at 1500 rpm for 5 mins and then washed once in 10 ml of thaw media. The thawed LPMCs were immediately resuspended in complete RPMI (RPMI + 10% human AB serum, 1% penicillin/streptomycin/glutamine, 500 μg/ml Zosyn) without any additional growth factors. HIV-1 (80 ng p24) per 1 M LPMCs was used to infect primary LPMCs at 10 M/ml in complete RPMI. LPMCs were mock infected in parallel. Infection by spinoculation was performed at 1200×g for 2 h at room temperature . After spinoculation, the supernatant containing free virus was discarded; the cell pellet was resuspended in complete RPMI at 1 M LPMCs/ml and then seeded in a 48-well plate. Heat-killed E. coli was added to LPMCs at a ratio of 5 E.coli: 1 LPMC where indicated. Fungizone (1.25 μg/ml) was added at 1 dpi. The LPMCs were harvested at indicated time points.
LPMC Infection in the presence of drugs
For infections in the presence of Efavirenz (ARRP Catalog #4624), LPMCs were pre-incubated with Efavirenz (1 nM or 10 nM) for 2 h at 37°C and infected as described above and cultured in media containing the indicated Efavirenz dose. A second dose of Efavirenz was added at 3 dpi. At 6 dpi, the LPMCs were collected and analyzed as described.
Inhibitors of Caspase-3 (DEVD-fmk; DEVD (Calbiochem)) and Caspase-1 (YVAD-cmk; YVAD (Calbiochem)) were used to inhibit PCD ex vivo in donor samples that showed depletion at 4 dpi. The YVAD motif is predominately cleaved by Caspase-1 but does have some cross reactivity (340-fold less affinity) with other group I caspases, Caspase-4 and Caspase-5 . The inhibitors were resuspended in DMSO at 50 mM and stored at −20°C per the manufacturer’s instructions. The dose used in primary LPMCs was based on optimization studies to balance maximum inhibition of the pathway with minimum toxicity. The 4 dpi time point was chosen so that consistent depletion was observed but DMSO exposure was minimal. Following HIV-1 spinoculation (0 dpi), the inhibitors were added to the appropriate wells at a 25 μM concentration. An additional dose of each inhibitor was added to the cultures at 2 dpi without changing the media. At 4 dpi, LPMCs were collected for analysis.
Mitogen Stimulation for Th Subset Identification
6 dpi LPMCs were collected, counted, resuspended at 1 M/ml in complete RPMI, and stimulated with 100 ng/ml PMA (Sigma-Aldrich), 100 μg/ml Ionomycin (Sigma- Aldrich) and 1 μg/ml Brefeldin A (Golgi Plug, BD Biosciences) for 5 h at 37°C in 5% CO2. LPMCs capable of making IFN-γ and IL-17 were identified by flow cytometry .
Antibodies for flow cytometry
Aqua Viability Dye (AqVi; Invitrogen) exclusion was used to identify viable cells. The antibodies used for these studies were: CD3-ECD (Beckman Coulter), CD8-APC (BD Pharmingen), CD19-APCH7 (BD Biosciences), HIV-1 p24-PE (Beckman Coulter), IL-17-V450 (BD biosciences), IFN-γ-AF700 (BD biosciences) and AnnexinV-Pacific Blue (Life Technologies). Isotype controls were used to establish the gates for IL-17 (Mouse IgG1- V450, BD Biosciences) and IFNγ (Mouse IgG1-AF700, BD biosciences).
Extracellular and Intracellular Staining
LPMCs were stained with cocktails of antibodies against markers expressed on the cell surface for 20 minutes in PBS + 1% BSA + 2 mM EDTA (FACS buffer) at 4°C. The LPMCs were then incubated with AqVi per the manufacturer’s instructions. The LPMCs were then fixed and permeabilized using the Invitrogen Medium A/Medium B system .
The best practices protocol from eBioscience was followed to detect AnnexinV + LPMCs in conjunction with intracellular markers incorporating minor modifications following the AqVi step in the standard protocol. After the LPMCs were incubated with AqVi, they were washed once with 2 ml of 1× AnnexinV binding buffer (BD Pharmingen). The LPMCs were resuspended in 1× AnnexinV binding buffer at a concentration of 1-3 M cells/ml and incubated, at room temperature for 15 min, with 5 μL of AnnexinV. Unbound AnnexinV was washed away using 2 ml 1× AnnexinV binding buffer. The cells were then fixed and stained for intracellular p24 using the Medium A/Medium B system. In optimization experiments, the early apoptotic phenotype was not detectable beyond 4 dpi. Therefore, we selected samples that consistently depleted at 4 dpi to determine the phenotype of the dying CD4+ T cells.
Fluorogenic Detection of Caspase-1 Activity
2 dpi and 6 dpi LPMCs were collected and counted using trypan blue. The LPMCs were resuspended in 75 uL of 10 mM CaspaLux-1 substrate (Oncoimmunin) and incubated for 30 mins at 37°C in 5% CO2. The LPMCs were washed once with 1 mL of the provided FACS buffer and surface stained as described above. The LPMCs were fixed with 4% PFA for 15 mins at room temperature, washed once with 2 mL of FACS buffer, and acquired in 1% PFA on the LSRII within 60 mins.
Flow cytometric acquisition and analysis
Acquisition occurred within 24 hours for all samples and within 2 h for samples stained with AnnexinV. All samples were collected on an LSR II flow cytometer (BD Biosciences) and analyzed using BD FACS DIVA version 6.1.3.
To quantify depletion, the number of viable T cells in an HIV-1 infected well was reported as a percent of the T cells in the mock infected control well at 4 or 6 dpi. The LPMCs were harvested and counted using trypan blue to determine the number of live cells in the well. However, because the LPAC model is a mixed cell culture model, the number of live cells counted by trypan blue does not equate to the number of viable CD4+ T cells per well. To determine the number of viable CD4+ T cells per well, flow cytometry was used to determine the percentage of the viable cells (analogous to the trypan blue count) that were CD3+CD8- in a tight lymphocyte gate that excluded both cellular debris and epithelial cells. The trypan blue count was then multiplied by the percentage of viable CD3+CD8- T cells measured by flow cytometry. For example, if 100,000 cells were counted by trypan blue and 40% were viable CD3+CD8- T cells by flow cytometry, we would report out 40,000 CD4+ T cells for that well. The same method was used to determine the number of Th1, Th17, Th1/17, and apoptotic or necrotic CD4+ T cells. CD4+ T cell depletion was determined in the presence and absence of E.coli.
Statistical analysis was conducted using GraphPad Prism version 5 for Windows (GraphPad, San Diego, CA). Non-parametric statistics were used in these analyses.
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CCW was supported by funding from the National Institutes of Health (R01 DK088663 and AI108404). MLS was supported by the University of Colorado Department of Medicine Early Career Scholar Program and startup funds. AKS was supported by funds from the Colorado HIV-1 Research Training Program (NIH 2T32AI007447-21). We acknowledge Zachary Dong, Daniel Hecht, and David Shugarts for their assistance with the study. Finally, we would like to acknowledge the AIDS reagent program for providing the virus stock and Efavirenz used in these studies.
The authors declare they have no competing interests.
AKS participated in the conceptual design and execution of the experiments, analyzed the data, and helped draft the manuscript; EJL and JAM provided technical support and critically reviewed the manuscript; SMD and JDB critically read the manuscript and participated in conceptual discussions; MDM provided critical access to the surgical samples; MLS and CCW participated in the conceptual design of the studies and helped draft the manuscript. All authors read and approved the final manuscript.