Skip to main content
Figure 1 | Retrovirology

Figure 1

From: Microbial exposure alters HIV-1-induced mucosal CD4+ T cell death pathways Ex vivo

Figure 1

HIV-1 mediated cell death can be quantified in the LPAC model. (A) Lamina Propria Aggregate Culture (LPAC) model. Primary LPMCs from discarded surgical samples were infected with CCR5-tropic HIV-1Ba-L (80 ng p24 per 1 M LPMC), cultured for 1–6 days and harvested for flow cytometry. (B) Representative gating strategy. (C) The absolute number of CD4+ T cells per well in mock or HIV-1 infected conditions. N = 17 donors. Each symbol is a unique donor. Significant differences at each time point was determined by Wilcoxon matched pairs signed rank test. (D) To compare relative depletion between donors, CD4+ T cell survival was normalized to mock infection (100% survival). Each symbol is an individual donor. Horizontal solid lines reflect the median. Significance was determined using the Kruskal-Wallis test across timepoints. (E) Cell-specificity of HIV-1 mediated depletion in the LPAC model. The percent survival relative to mock infection at 6 dpi was determined for CD3+CD8- T cells (white), CD3+CD8+ T cells (light grey), and CD3-CD19+ B cells (dark grey). The black bar depicts 100% survival for each cell type in the mock infected condition, also indicated by the dashed horizontal line. Significance was determined using the Kruskal-Wallis test and Dunn’s multiple comparison test to compare selected pairs of columns. **p = 0.0015; ***p = 0.0003; ****p < 0.0001.

Back to article page