Figure 3
From: Microbial exposure alters HIV-1-induced mucosal CD4+ T cell death pathways Ex vivo
HIV-1 infection increases cell death markers in LP CD4+ T cells. (A) Representative flow plot of AnnexinV/AqVi staining. Note that in contrast to Figure 1B, there was no AqVi exclusion gate prior to identifying lymphocytes from the FSC/SSC profile. Cells expressing AnnexinV were considered committed to dying. (B) The percentage of CD3+CD8- T cells expressing either an apoptotic (left) or necrotic (right) profiles were compared between mock and HIV-1 infected conditions. Each symbol is a unique donor. The fold differences in median expression are shown. Significance was determined using the Wilcoxon matched-pairs signed rank test. (C) The numbers of p24+ and p24neg cells per well with an apoptotic or necrotic phenotype were calculated. Each symbol represents a unique donor. The median values are shown and the fold-change is indicated for significant differences. Significance was determined using the Wilcoxon matched-pairs signed rank test. (D) Apoptotic versus necrotic p24+ and p24neg cells as a percentage of the total number of LP CD3+CD8- T cells committed to death (n = 11). (E-F) Spearman rank correlation analysis between the number of AnnexinV+ (E) p24neg and (F) p24+ cells 4 dpi versus the 6 dpi depletion levels. The best-fit lines, Spearman r and p values are indicated. **, p = 0.009; ***, p = 0.001.