Figure 6
From: Microbial exposure alters HIV-1-induced mucosal CD4+ T cell death pathways Ex vivo
E. coli alters caspase-dependent LP CD4+ T cell death. (A and B) Impact of E.coli exposure on apoptotic and necrotic phenotypes in the LPAC model. The absolute numbers of p24+ cells that were either apoptotic (left) or necrotic (right) were determined as in Figure 3 for (A) p24+ and (B) p24neg cells. Each symbol represents a unique donor. Significance was determined using the Wilcoxon matched-pairs signed rank test within each phenotype. (C) Apoptotic versus necrotic p24+ or p24neg cells as a percentage of the total LP CD3+CD8- T cells committed to death (n = 11). Compare to Figure 4B. (D) Impact of E.coli on cell death markers in uninfected LPMCs. The percentage of apoptotic or necrotic mock-infected cells were compared with or without E.coli. The horizontal lines indicate the median values and the fold-differences in cell death marker expression. Significance was determined using the Wilcoxon matched-pairs signed rank test within each phenotype. (E) The percent depletion from mock infection in the presence of YVAD and DEVD (n = 7) as described in Figure 5 in the presence of E.coli. Overall ANOVA, p = 0.008. *p = < 0.05 **, p = 0.007; ***, p = 0.001.