- Open Access
Implication of TRIMalpha and TRIMCyp in interferon-induced anti-retroviral restriction activities
© Carthagena et al; licensee BioMed Central Ltd. 2008
- Received: 29 April 2008
- Accepted: 09 July 2008
- Published: 09 July 2008
TRIM5α is a restriction factor that interferes with retroviral infections in a species-specific manner in primate cells. Although TRIM5α is constitutively expressed, its expression has been shown to be up-regulated by type I interferon (IFN). Among primates, a particular case exists in owl monkey cells, which express a fusion protein between TRIM5 and cyclophilin A, TRIMCyp, specifically interfering with HIV-1 infection. No studies have been conducted so far concerning the possible induction of TRIMCyp by IFN. We investigated the consequences of IFN treatment on retroviral restriction in diverse primate cells and evaluated the implication of TRIM5α or TRIMCyp in IFN-induced anti-retroviral activities.
First, we show that human type I IFN can enhance TRIM5α expression in human, African green monkey and macaque cells, as well as TRIMCyp expression in owl monkey cells. In TRIM5α-expressing primate cell lines, type I IFN has little or no effect on HIV-1 infection, whereas it potentates restriction activity against N-MLV in human and African green monkey cells. In contrast, type I IFN treatment of owl monkey cells induces a great enhancement of HIV-1 restriction, as well as a strain-tropism independent restriction of MLV. We were able to demonstrate that TRIM5α is the main mediator of the IFN-induced activity against N-MLV in human and African green monkey cells, whereas TRIMCyp mediates the IFN-induced HIV-1 restriction enhancement in owl monkey cells. In contrast, the type I IFN-induced anti-MLV restriction in owl monkey cells is independent of TRIMCyp expression.
Together, our observations indicate that both TRIM5α and TRIMCyp are implicated in IFN-induced anti-retroviral response in primate cells. Furthermore, we found that type I IFN also induces a TRIMCyp-independent restriction activity specific to MLV in owl monkey cells.
- Vero Cell
- Feline Immunodeficiency Virus
- African Green Monkey Cell
In response to infections, eukaryotes have evolved a wide variety of defense mechanisms. In addition to classical innate and adaptive immunities, a third mode of immunity specific to retroviral infections has recently been identified and termed "intrinsic immunity" . So far, two classes of cellular proteins that specifically interfere with retroviral infections at the cellular level have been identified. The first class of factors is constituted of cytidine deaminases such as APOBEC3G, which induce lethal hypermutation of retroviral genomes (reviewed in [2, 3]). The second class of retroviral restriction factors targets capsid proteins of incoming virions and comprises the murine Fv1 and the primate TRIM5α proteins (reviewed in [2, 3]).
TRIM5α protein from Old World monkeys blocks both human immunodeficiency virus type 1 (HIV-1) and N-tropic murine leukemia virus (N-MLV), whereas human TRIM5α only interferes with N-MLV infection. Most TRIM5α variants from New World monkeys restrict simian immunodeficiency virus (SIVmac) but not HIV-1 infection , with the notable exception of owl monkey (Aotus trivigartus) cells which block HIV-1 but not SIVmac or N-MLV infections. Instead of TRIM5α, owl monkeys express a TRIMCyp fusion protein, generated by retrotransposition of a cyclophilin A (CypA) mRNA within the TRIM5 locus [14, 15]. This retrotransposition event led to the expression of a chimeric protein that consists of the RBCC motif of TRIM5 fused to a carboxy-terminal CypA moiety [14, 15] (Figure 1A). CypA is a ubiquitously expressed and highly conserved peptidyl prolyl isomerase that can catalyze cis/trans-isomerization of prolyl peptide bonds. This cellular protein has been shown to bind the HIV-1 capsid protein (CA) through a direct interaction between the active site of CypA and an exposed loop within the CA protein, known as the CypA binding loop [16, 17]. Capsid proteins from other retroviruses also interact with CypA, such as feline immunodeficiency virus (FIV), SIVcpz or SIVagm, whereas others such as MLV or SIVmac, do not [18, 19]. CypA-CA interaction can be disrupted by cyclosporine A (CsA), an immunosuppressive drug that competitively binds to the CypA active site [17, 20]. In consequence, since owl monkey TRIMCyp proteins bind CA proteins of incoming retroviruses through their C-terminal CypA domain, restriction can be relieved by CsA treatment [14, 15, 21, 22].
Like many other members of its protein family [23, 24], TRIM5α expression has been recently found to be up-regulated by type I interferon (IFN) in human cells, through an IFN-stimulated response element (ISRE) within its promoter . This finding suggested that IFN might influence the retroviral activity of TRIM5α. In the particular case of owl monkey cells, it is not known whether TRIMCyp expression is also enhanced by IFN. We thus tried to determine whether the retroviral restriction profile of different primate cells can be modulated by type I (α, β) and type II (γ) IFNs, and to evaluate the role of TRIM5α and TRIMCyp in IFN-induced anti-retroviral responses.
Cells and viruses
Cell lines of human (Homo sapiens, HeLa cells), African green monkey (Cercopithecus aethiops, Vero cells), rhesus macaque (Macaca mulatta, CMMT cells), and owl monkey (Aotus trivigartus, OMK cells) origin were maintained in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum and antibiotics.
Vesicular stomatitis virus glycoprotein (VSV-G)-pseudotyped retroviral vectors were generated by co-transfecting 10-cm plates of 293T cells with 10 μg of pVSV-G, 10 μg of Gag-Pol expression plasmid and 10 μg of green fluorescent protein (GFP)-expressing retroviral vector, using the ProFection calcium phosphate kit (Promega). MLV vectors were made with pCFG2-eYFP and pHIT60 (Mo-MLV, a NB-tropic strain), pCIG3N (N-tropic MLV), or pCIG3B (B-tropic MLV). HIV-1 vectors were prepared with pCSGW (GFP vector) and p8.91 (HIV-1 Gag-Pol). All MLV- and HIV-derived plasmids were kindly provided by J.P. Stoye (National Institute for Medical Research, London, UK). SIVmac vectors were generated with pAd-SIV3+ and GAE-CMV-GFP, which were kindly provided by F.L. Cosset (ENS Lyon, France). The HIV SCA Gag-Pol plasmid was a generous gift from J. Sodroski (Dana Farber Cancer Institute, Boston, MA, USA) and was co-transfected with pCSGW, pVSV-G and pRev (10:10:8:2 ratio) to produce HIV-1 SCA viral particles. All viral stocks were titrated on Mus dunni tail fibroblast (MDTF) cells and analyzed by FACS. The multiplicity of infection (MOI) is defined as MOI = -2 ln(1-fp), where fp is the fraction of GFP-positive MDTF cells. HeLa, Vero, CMMT or OMK cells were then transduced with increasing doses of GFP-encoding retroviral vectors and the percentage of transduced GFP-positive cells was determined by FACS 48 h post-transduction [7, 8].
Drug and interferon treatment
Cyclosporin A (CsA, Sigma) was prepared at 1 mM in ethanol and diluted further in culture medium before use. For all experiments, IFN-α, IFN-β or IFN-γ were used at 1000 units/ml (U/ml). Human recombinants IFN-α2 is from Schering-Plough, IFN-β is from Cytotech SA and IFN-γ is from Roussel Uclaf. For treating MDTF cells, we used universal type I IFN (PBL Biomedical Laboratories) at 200 U/ml, which was found to be active on most mammalian cells.
Total RNA was extracted using RNeasy Mini Kit (Qiagen) and cDNA were prepared using a Oligo(dT) primer and SuperScript III Reverse Transcriptase (Invitrogen). Quantitative PCR were performed in duplicates using 1 μl of cDNA on a Roche LightCycler, using the LightCycler Fast Start DNA Master SYBR Green 1 kit (Roche). Primers were synthesized by Eurogentec. TRIM5α: TTGGATCCTGGGGGTATGTGCTGG (forward) and TGATATTGAAGAATGAGACAGTGCAAG (reverse). GAPDH: GGGAAACTGTGGCGTGAT (forward) and GGAGGAGTGGGTGTCGCTGTT (reverse). CypA: AGTGGTTGGATGGCAAGC (forward) and GATTCTAGGATACTGCGAGCAAA (reverse). TRIMCyp: CAGAAGTCCAACGCTACTGGG (forward) and CTTGCCACCAGTGCCATTATGG (reverse). PCR reactions were carried out with a denaturation step of 10 min at 95°C followed by forty-five cycles of 10 s at 95°C, 5 s at annealing temperature (55°C for cyclophilin A, 60°C for TRIM5α, TRIMCyp and GAPDH) and 20 s amplification at 72°C. Quantifications of cDNAs were determined in reference to a standard curve prepared by amplification of serial dilutions of PCR product or plasmids containing matching sequences. Analyses were performed using the second-derivative-maximum method provided by the LightCycler quantification software, version 3.5 (Roche Diagnostics).
Western blot analysis
Cells untreated or treated with IFN-α, β or γ at 1000 U/ml (or with 200 U/ml of universal type I IFN in the case of MDTF cells) for 20 min were lysed with lysis buffer (20 mM Tris HCl pH7.5, 400 mM NaCl, 1% Triton, 1 mM EDTA, 50 mM KCl and 5 mM β-Mercaptoethanol). Cells extracts (100 μg) were resolved by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and western blotted using anti-phosphorylated Stat1 (Tyr 701) rabbit antibodies (Santa Cruz Biotechnology, Inc.) or an anti-actin mouse mAb (Calbiochem).
Down-regulation of TRIM5α or TRIMCyp expression by siRNA was performed by transfecting cells with siRNA oligos (Dharmacon) using HiPerFect Transfection Reagent (Qiagen) according to manufacturer's instructions. siRNAs targeting human TRIM5α were H1 (GGUCAUUUGCUGGCUUUGU) and HA2 (GCACUGUCUCAUUCUUCAA). For African green monkey (agm) TRIM5α silencing, we used HA2 and A3 (GCCUUACGAAGUCUGAAAC). In order to silence TRIMCyp expression in OMK cells, we used a siRNA targeting CypA (CypA: GGGUUCCUGCUUUCACAGA). Control siRNA transfections were performed using a luciferase control siRNA (Dharmacon).
Quantification of reverse transcripts
OMK cells untreated or treated with 1000 U/ml of IFN-β were transduced 24 h later with GFP-expressing retroviral vectors pre-treated with DNase (Roche DNase I RNase-free 200 U/ml, 10 mM MgCl2, 1 h at room temperature) and harvested 6 h later. Total DNA was extracted using DNeasy Mini Kit (Qiagen) and quantified by measuring OD at 260 nm. Reverse transcripts were detected by PCR on 100 ng of total DNA using primers to GFP: TACGGCAAGCTGACCCTGAAG (forward) and ACGAACTCCAGCAGGACCATG (reverse).
Up-regulation of TRIM5α and TRIMCyp expression by IFNs in primate cells
To investigate whether type I and type II IFNs can enhance TRIM5 (TRIM5α or TRIMCyp) expression in various primate cells, we decided to compare cell lines of human (HeLa), African green monkey (Vero), rhesus macaque (CMMT) and owl monkey (OMK) origin.
First, we compared the constitutive expression of TRIM5α (HeLa, Vero and CMMT cells) or TRIMCyp (OMK cells) transcripts in the different cell lines by quantitative RT-PCR (Figure 1B). We found that OMK cells contain approximately 1.5 × 107 copies/μg total RNA of TRIMCyp transcripts, whereas HeLa and Vero cells contain 3 × 107 transcripts encoding TRIM5α. Among the four cell lines tested, CMMT cells express the highest number of TRIM5 transcripts, with a concentration of 9 × 107 copies of TRIM5α per μg of total RNA.
In order to verify if non-human primate cells are responsive to human IFN treatment, we looked at Stat1 phosphorylation, which is an early post-receptor signaling element of both type I and type II IFN pathways, and can thus serve as a positive-indicator of IFN signaling . For this purpose, CMMT, Vero and OMK cells were treated with 1000 U/ml of human IFN-α, β or γ for 20 min and the activation of Stat1 was estimated by western blotting using anti-phosphorylated Stat1 antibodies. As shown in Figure 1C, although the different cell lines do not equally respond to the three IFNs, both human type I (α, β) and II (γ) IFNs can induce Stat1 phosphorylation in non-human primate cells as well as in human cells (HeLa cells). As a control, the same assay was performed on MDTF cells treated with 200 U/ml of universal type I IFN (Fig 1C), which is active on most mammalian cells. MDTF cells are devoid of any post-entry retroviral restricting activity, since they are Fv1-null [27, 28], do not encode a TRIM5 ortholog, like all other mouse cells (reviewed in ), and were found to express an inactive APOBEC3G protein.
Next, we stimulated the primate cell lines with 1000 U/ml of IFN-α, β or γ for 8 h and determined TRIM5α or TRIMCyp mRNA amounts by quantitative RT-PCR. Results were normalized to GAPDH mRNA levels. Figure 1D shows the ratio of TRIM5α or TRIMCyp expression in IFN treated/untreated cell, which is referred to as "Fold increase". We found that type I IFN enhances TRIM5α mRNA expression with induction folds ranging from 3.5 (CMMT) to 7.6 (HeLa) for IFN-α, and from 2.8 (CMMT) to 8.8 (HeLa) for IFN-β. In contrast, IFN-γ treatment does not significantly affect TRIM5α expression in any cell line, with fold induction values ranging from 1.4 (Vero) to 1.9 (CMMT). These results are consistent with previous observations made in human cells . In OMK cells, stimulation by type I IFN leads to a 4.4 (IFN-α) to 6.6 (IFN-β) fold increase of TRIMCyp mRNA expression, whereas type II IFN does not have any significant effect (around 1.3 fold increase).
Effect of IFNs on retroviral restriction in primate cells
In the absence of IFN treatment, N-MLV is restricted in HeLa and Vero cells, whereas HIV-1 is blocked in CMMT and Vero cells. As expected, NB-MLV infection is not affected in any cell line. Furthermore, IFN-treatment of MDTF cells, which are devoid of any restriction factor, does not affect their capacity to be transduced by any retroviral vector. We did not observe any dramatic effect on retroviral restriction levels in IFN-treated primate cells at this low multiplicity of infection. IFN-β was found to be the most effective in enhancing TRIM5α-mediated restriction, in accordance with the results of expression enhancement. Following IFN-β treatment, we observed a low but reproducible enhancement of N-MLV restriction in HeLa and Vero cells, as well as a higher restriction towards HIV-1 infection in CMMT cells. This moderate effect of IFN at low MOI is probably due to the fact that the constitutive amount of TRIM5α in the cells is sufficient to restrict a small amount of virus, whereas at higher MOI, it can be predicted that an increased expression of TRIM5α would prevent its saturation by incoming viruses, thus enhancing restriction. Indeed, when a higher dose of virus was used (MOI of 5 on MDTF cells), we observed a much higher restriction efficiency of N-MLV in HeLa and Vero cells treated with type I IFN (Figure 2). In contrast, no significant increase of HIV-1 restriction can be observed in Vero or CMMT cells.
Together, our observations confirm that type I IFN can modulate retroviral restriction in diverse primate cells. However, we did not observe a direct and systematic correlation between the levels of up-regulation of TRIM5α expression and the enhancement of restriction activity. Indeed, the IFN-induced reduction of infectivity of TRIM5α-sensitive viruses seems to be dependent of diverse parameters, such as the multiplicity of infection and the basal restriction activity of cells against a given virus.
Effect of IFN-β treatment on retroviral restriction.
Therefore, our observations suggest that OMK cells express an IFN-inducible anti-retroviral protein other than TRIMCyp, which can interfere specifically with certain retroviruses.
The IFN-induced anti-retroviral activity in HeLa and Vero cells is TRIM5α-dependent
The IFN-induced anti-MLV activity in OMK cells is TRIMCyp-independent
This was further confirmed by comparing the restriction profile of HIV-1, SIVmac and HIV-1 containing the CA protein of SIVmac (HIV SCA) following IFN-β stimulation of OMK cells. As shown in Figure 6B, the HIV SCA chimera virus is as insensitive to IFN-β treatment as SIVmac, compared to HIV-1.
The IFN-induced block in OMK cells targets an early step of MLV replication
Since IFNs can induce several antiviral mediators acting at different stages of viral replication, we next examined which step of the viral cycle is inhibited in response to IFN treatment of OMK cells.
In this study, we have shown that type I (α and β) IFN increases TRIM5α expression in human, African green monkey and rhesus macaque cell lines, as well as TRIMCyp expression in owl monkey cells. A previous study reported that human TRIM5α expression can be directly up-regulated by IFN-β through a putative interferon-stimulated response element (ISRE) within its promoter . Our data are in agreement with the results published by Asaoka et al. , as we observed in HeLa cells an 8- to 9-fold increase of TRIM5α expression with IFN-β and no effect with IFN-γ. In contrast, we found that IFN-α was almost as efficient as IFN-β in stimulating human TRIM5α expression, in agreement with another recent study . It should be noted that the TRIM5α expression increase in CMMT cells is not as strong as in the other primate cells, with a 3- to 3.5-fold increase following stimulation by IFN-α or IFN-β, respectively.
We found that this type I IFN enhancement of TRIM5α expression can influence cell permissivity towards retroviral infections in diverse primate cells. In most cases, we observed a greater effect of IFN stimulation when high concentrations of virus were used. This probably reflects the fact that the constitutive amount of TRIM5α is sufficient to block a small number of incoming viruses, whereas at high MOI, an enhancement of TRIM5α intracellular concentration following IFN treatment facilitates restriction by preventing TRIM5α saturation.
In human cells, IFN-β treatment increases TRIM5α expression by approximately 9-fold, and leads to a 7- to 8-fold reduction of N-MLV permissivity. In contrast, IFN treatment does not affect the permissivity of TRIM5αhu-insensitive viruses, such as NB-MLV or HIV-1. These first observations confirm a previous report showing that IFN-α treatment of human cells both increases the level of TRIM5α mRNA and potentates N-MLV restriction by approximately 5-fold .
In the case of CMMT and Vero cells, TRIM5α expression is also up-regulated following type I IFN treatment, but in these cases, the increase of expression does not strictly correlate with a decreased infectivity of TRIM5α-sensitive viruses.
In rhesus macaque cells, at low MOI, we only observed a moderate decrease of HIV-1 infectivity (2- to 3-fold reduction), in agreement with Sakuma et al. . In contrast, no effect on N-MLV infection can be observed. Surprisingly, when a higher MOI was used, IFN-treated CMMT cells showed no difference of susceptibility to either N-MLV or HIV-1 infection, compared to untreated cells. This relative insensitivity of CMMT to IFN treatment probably reflects the fact that these cells constitutively express high amount of TRIM5α (Figure 1B) compared to the other primate cell lines. Furthermore, this high constitutive expression is conjugated to a moderate enhancement of TRIM5α expression in response to IFN (Figure 1D).
In Vero cells, we detected no effect of type I or type II IFNs at low MOI, whereas at high MOI, we observed an enhancement of N-MLV restriction following induction by type I IFN only. Unlike N-MLV, HIV-1 infectivity was not found to be significantly affected by the IFN-induced up-regulation of TRIM5α expression. We believe that this apparent discrepancy may be explained by the basal restriction phenotype in Vero cells. Indeed, at constitutive levels of TRIM5α expression, Vero cells restrict N-MLV infection much more efficiently than HIV-1, suggesting that their TRIM5α proteins recognize N-MLV CA better than HIV-1 CA. Our observations suggest that IFN stimulation can potentate restriction towards highly sensitive viruses, but is not sufficient to enhance the restriction activity towards less sensitive ones.
Importantly, we were able to demonstrate that the IFN-induced restriction increase towards N-MLV observed in human and African green monkey cells was mainly due to the enhancement of TRIM5α expression, demonstrating that TRIM5α is the main mediator of the anti-retroviral activity of type I IFN in these cells.
Several studies have demonstrated that IFN-α can interfere with early and late steps of HIV-1 replication, in vitro [32–35]. Although we have only tested the effect of IFN on HeLa cells rather than primary blood cells, our results are not in favor of a direct implication of TRIM5α in the early anti-HIV-1 block induced by IFN in human cells. Indeed, up-regulation of TRIM5α expression by type I IFN was not found to correlate with a significant decrease in HIV-1 permissivity. In this context, APOBEC3G, another cellular IFN-induced anti-retroviral factor, is more likely to play a role as a mediator of the early IFN-α-mediated anti-HIV block in human cells , whereas the possible involvement of TRIM5α in the late steps remains to be addressed .
The second part of our study focused on the characterization of IFN-induced modulation of retroviral infection susceptibility in owl monkey cells. Cells derived from this New World monkey species express a TRIMCyp fusion protein which allows them to specifically interfere with viruses whose capsid can bind CypA, such as HIV-1, FIV and SIVagm [18, 22]. In contrast, MLV and SIVmac capsid proteins do not bind CypA and these viruses are in consequence insensitive to TRIMCyp restriction.
First, we observed a 3 (at a MOI of 0.5) to 26-fold (at a MOI of 5) reduction of HIV-1 permissivity following stimulation of OMK cells with type I IFN, which can be attributed almost entirely to the IFN-induced up-regulation of TRIMCyp expression.
Surprisingly, in addition to this strong enhancement of HIV-1 restriction following type I IFN stimulation of OMK cells, we also observed a significant and reproducible restriction of MLV. This MLV block is independent of the strain tropism, since N-, B- and NB-tropic MLV strains were found to be sensitive. Many mammalian cells are able to restrict MLV, but in all cases, the block only affects N-tropic strains , with the exception of murine NIH-3T3 cells which express a B-tropic MLV-specific restriction factor, known as Fv1n (reviewed in [38, 39]). We were able to demonstrate that this IFN-induced anti-MLV activity is independent of TRIMCyp expression and occurs early during the MLV replication cycle, before or during the reverse transcription step. In contrast to MLV, we found that SIVmac was not affected by IFN-treatment in OMK cells. Even though the effect of IFN-treatment in OMK cells has never been investigated, this observation was unexpected. Indeed, in human cells, IFN-treatment was found to block an early step of SIVmac replication, between viral entry and reverse transcription [40, 41]. Furthermore, IFNs are known to activate multiple antiviral proteins which induce a wide-spectrum antiviral response. Thus, our results suggest the absence of IFN-induced antiviral response against SIVmac in OMK cells.
Several cellular proteins have been identified as mediators of the antiviral activity of IFNs, such as Protein Kinase RNA-dependent, 2'5' oligoadenylate synthetase/RNase L, and certain Mx proteins (reviewed in ). Interestingly, in addition to these well characterized IFN-induced antiviral mediators, some other proteins belonging to the TRIM protein family have also been involved in IFN-induced antiviral defense, such as TRIM5α [25, 31], PML/TRIM19 (for review, see [43, 44]), TRIM22 [45, 46] or TRIM25 . These observations suggest that the entire TRIM family may constitute a family of antiviral factors implicated in the IFN-induced intracellular innate immunity . In this respect, a systematic study of the expression of mouse TRIM genes in immune cells in response to various stimuli, including IFN treatment and viral infection, provides new insights into the implication of the TRIM family in antiviral defense. The number of TRIM proteins up-regulated by IFN and/or implicated in antiviral resistance raises the possibility of the involvement of a TRIM protein other than TRIMCyp in the IFN-induced anti-MLV restriction we observed in OMK cells. Apart from TRIMCyp, the only TRIM protein that has been characterized so far in OMK cells is TRIM1 . However, since it was found to interfere specifically with N- but not B-tropic strains of MLV, this protein is unlikely to play a role in the observed phenotype. Whether it belongs to the TRIM protein family or not, the identification of the mediator(s) of the IFN-induced anti-MLV restriction in owl monkey cells will need further investigation.
We thank Joseph Sodroski and François-Loïc Cosset for the gift of reagents and Jonathan Stoye for sharing reagents and for helpful discussions. We also thank Laura Burleigh for correcting the English in the manuscript. This work was supported by a grant from Sidaction and two grants (attributed to SN and MKCA) from the Agence Nationale de Recherche contre le SIDA et les hépatites virales (ANRS). LC and MCP are supported by grants from Le Ministère de l'Enseignement Supérieur et de la Recherche (MESR).
- Bieniasz PD: Intrinsic immunity: a front-line defense against viral attack. Nat Immunol. 2004, 5 (11): 1109-1115. 10.1038/ni1125.View ArticlePubMedGoogle Scholar
- Baumann JG: Intracellular restriction factors in mammalian cells--An ancient defense system finds a modern foe. Curr HIV Res. 2006, 4 (2): 141-168. 10.2174/157016206776055093.View ArticlePubMedGoogle Scholar
- Perez O, Hope TJ: Cellular restriction factors affecting the early stages of HIV replication. Curr HIV/AIDS Rep. 2006, 3 (1): 20-25. 10.1007/s11904-006-0004-3.View ArticlePubMedGoogle Scholar
- Hatziioannou T, Perez-Caballero D, Yang A, Cowan S, Bieniasz PD: Retrovirus resistance factors Ref1 and Lv1 are species-specific variants of TRIM5alpha. Proc Natl Acad Sci U S A. 2004, 101 (29): 10774-10779. 10.1073/pnas.0402361101.PubMed CentralView ArticlePubMedGoogle Scholar
- Keckesova Z, Ylinen LM, Towers GJ: The human and African green monkey TRIM5alpha genes encode Ref1 and Lv1 retroviral restriction factor activities. Proc Natl Acad Sci U S A. 2004, 101 (29): 10780-10785. 10.1073/pnas.0402474101.PubMed CentralView ArticlePubMedGoogle Scholar
- Perron MJ, Stremlau M, Song B, Ulm W, Mulligan RC, Sodroski J: TRIM5alpha mediates the postentry block to N-tropic murine leukemia viruses in human cells. Proc Natl Acad Sci U S A. 2004, 101 (32): 11827-11832. 10.1073/pnas.0403364101.PubMed CentralView ArticlePubMedGoogle Scholar
- Stremlau M, Owens CM, Perron MJ, Kiessling M, Autissier P, Sodroski J: The cytoplasmic body component TRIM5alpha restricts HIV-1 infection in Old World monkeys. Nature. 2004, 427: 848-853. 10.1038/nature02343.View ArticlePubMedGoogle Scholar
- Yap MW, Nisole S, Lynch C, Stoye JP: Trim5alpha protein restricts both HIV-1 and murine leukemia virus. Proc Natl Acad Sci U S A. 2004, 101 (29): 10786-10791. 10.1073/pnas.0402876101.PubMed CentralView ArticlePubMedGoogle Scholar
- Nisole S, Stoye JP, Saib A: TRIM family proteins: retroviral restriction and antiviral defence. Nat Rev Microbiol. 2005, 3 (10): 799-808. 10.1038/nrmicro1248.View ArticlePubMedGoogle Scholar
- Reymond A, Meroni G, Fantozzi A, Merla G, Cairo S, Luzi L, Riganelli D, Zanaria E, Messali S, Cainarca S, Guffanti A, Minucci S, Pelicci PG, Ballabio A: The tripartite motif family identifies cell compartments. Embo J. 2001, 20 (9): 2140-2151. 10.1093/emboj/20.9.2140.PubMed CentralView ArticlePubMedGoogle Scholar
- Sawyer SL, Wu LI, Emerman M, Malik HS: Positive selection of primate TRIM5alpha identifies a critical species-specific retroviral restriction domain. Proc Natl Acad Sci U S A. 2005Google Scholar
- Yap MW, Nisole S, Stoye JP: A Single Amino Acid Change in the SPRY Domain of Human Trim5alpha Leads to HIV-1 Restriction. Curr Biol. 2005, 15 (1): 73-78. 10.1016/j.cub.2004.12.042.View ArticlePubMedGoogle Scholar
- Song B, Javanbakht H, Perron M, Park do H, Stremlau M, Sodroski J: Retrovirus restriction by TRIM5alpha variants from Old World and New World primates. J Virol. 2005, 79 (7): 3930-3937. 10.1128/JVI.79.7.3930-3937.2005.PubMed CentralView ArticlePubMedGoogle Scholar
- Nisole S, Lynch C, Stoye JP, Yap MW: A Trim5-cyclophilin A fusion protein found in owl monkey kidney cells can restrict HIV-1. Proc Natl Acad Sci U S A. 2004, 101 (36): 13324-13328. 10.1073/pnas.0404640101.PubMed CentralView ArticlePubMedGoogle Scholar
- Sayah DM, Sokolskaja E, Berthoux L, Luban J: Cyclophilin A retrotransposition into TRIM5 explains owl monkey resistance to HIV-1. Nature. 2004, 430 (6999): 569-573. 10.1038/nature02777.View ArticlePubMedGoogle Scholar
- Gamble TR, Vajdos FF, Yoo S, Worthylake DK, Houseweart M, Sundquist WI, Hill CP: Crystal structure of human cyclophilin A bound to the amino-terminal domain of HIV-1 capsid. Cell. 1996, 87 (7): 1285-1294. 10.1016/S0092-8674(00)81823-1.View ArticlePubMedGoogle Scholar
- Luban J, Bossolt KL, Franke EK, Kalpana GV, Goff SP: Human immunodeficiency virus type 1 Gag protein binds to cyclophilins A and B. Cell. 1993, 73 (6): 1067-1078. 10.1016/0092-8674(93)90637-6.View ArticlePubMedGoogle Scholar
- Lin TY, Emerman M: Cyclophilin A interacts with diverse lentiviral capsids. Retrovirology. 2006, 3: 70-10.1186/1742-4690-3-70.PubMed CentralView ArticlePubMedGoogle Scholar
- Franke EK, Yuan HE, Luban J: Specific incorporation of cyclophilin A into HIV-1 virions. Nature. 1994, 372 (6504): 359-362. 10.1038/372359a0.View ArticlePubMedGoogle Scholar
- Braaten D, Aberham C, Franke EK, Yin L, Phares W, Luban J: Cyclosporine A-resistant human immunodeficiency virus type 1 mutants demonstrate that Gag encodes the functional target of cyclophilin A. J Virol. 1996, 70 (8): 5170-5176.PubMed CentralPubMedGoogle Scholar
- Towers GJ, Hatziioannou T, Cowan S, Goff SP, Luban J, Bieniasz PD: Cyclophilin A modulates the sensitivity of HIV-1 to host restriction factors. Nat Med. 2003, 9 (9): 1138-1143. 10.1038/nm910.View ArticlePubMedGoogle Scholar
- Diaz-Griffero F, Vandegraaff N, Li Y, McGee-Estrada K, Stremlau M, Welikala S, Si Z, Engelman A, Sodroski J: Requirements for capsid-binding and an effector function in TRIMCyp-mediated restriction of HIV-1. Virology. 2006, 351 (2): 404-419. 10.1016/j.virol.2006.03.023.View ArticlePubMedGoogle Scholar
- Chelbi-Alix MK, Pelicano L, Quignon F, Koken MH, Venturini L, Stadler M, Pavlovic J, Degos L, de The H: Induction of the PML protein by interferons in normal and APL cells. Leukemia. 1995, 9 (12): 2027-2033.PubMedGoogle Scholar
- Stadler M, Chelbi-Alix MK, Koken MH, Venturini L, Lee C, Saib A, Quignon F, Pelicano L, Guillemin MC, Schindler C, et al: Transcriptional induction of the PML growth suppressor gene by interferons is mediated through an ISRE and a GAS element. Oncogene. 1995, 11 (12): 2565-2573.PubMedGoogle Scholar
- Asaoka K, Ikeda K, Hishinuma T, Horie-Inoue K, Takeda S, Inoue S: A retrovirus restriction factor TRIM5alpha is transcriptionally regulated by interferons. Biochem Biophys Res Commun. 2005, 338 (4): 1950-1956. 10.1016/j.bbrc.2005.10.173.View ArticlePubMedGoogle Scholar
- Darnell JE, Kerr IM, Stark GR: Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins. Science. 1994, 264 (5164): 1415-1421. 10.1126/science.8197455.View ArticlePubMedGoogle Scholar
- Bock M, Bishop KN, Towers G, Stoye JP: Use of a transient assay for studying the genetic determinants of Fv1 restriction. J Virol. 2000, 74 (16): 7422-7430. 10.1128/JVI.74.16.7422-7430.2000.PubMed CentralView ArticlePubMedGoogle Scholar
- Hartley JW, Rowe WP: Clonal cells lines from a feral mouse embryo which lack host-range restrictions for murine leukemia viruses. Virology. 1975, 65 (1): 128-134. 10.1016/0042-6822(75)90013-6.View ArticlePubMedGoogle Scholar
- Lee K, KewalRamani VN: In defense of the cell: TRIM5alpha interception of mammalian retroviruses. Proc Natl Acad Sci U S A. 2004, 101 (29): 10496-10497. 10.1073/pnas.0404066101.PubMed CentralView ArticlePubMedGoogle Scholar
- Mariani R, Chen D, Schrofelbauer B, Navarro F, Konig R, Bollman B, Munk C, Nymark-McMahon H, Landau NR: Species-specific exclusion of APOBEC3G from HIV-1 virions by Vif. Cell. 2003, 114 (1): 21-31. 10.1016/S0092-8674(03)00515-4.View ArticlePubMedGoogle Scholar
- Sakuma R, Mael AA, Ikeda Y: Alpha interferon enhances TRIM5alpha-mediated antiviral activities in human and rhesus monkey cells. J Virol. 2007, 81 (18): 10201-10206. 10.1128/JVI.00419-07.PubMed CentralView ArticlePubMedGoogle Scholar
- Coccia EM, Krust B, Hovanessian AG: Specific inhibition of viral protein synthesis in HIV-infected cells in response to interferon treatment. J Biol Chem. 1994, 269 (37): 23087-23094.PubMedGoogle Scholar
- Poli G, Orenstein JM, Kinter A, Folks TM, Fauci AS: Interferon-alpha but not AZT suppresses HIV expression in chronically infected cell lines. Science. 1989, 244 (4904): 575-577. 10.1126/science.2470148.View ArticlePubMedGoogle Scholar
- Shirazi Y, Pitha PM: Alpha interferon inhibits early stages of the human immunodeficiency virus type 1 replication cycle. J Virol. 1992, 66 (3): 1321-1328.PubMed CentralPubMedGoogle Scholar
- Fernie BF, Poli G, Fauci AS: Alpha interferon suppresses virion but not soluble human immunodeficiency virus antigen production in chronically infected T-lymphocytic cells. J Virol. 1991, 65 (7): 3968-3971.PubMed CentralPubMedGoogle Scholar
- Chen K, Huang J, Zhang C, Huang S, Nunnari G, Wang FX, Tong X, Gao L, Nikisher K, Zhang H: Alpha interferon potently enhances the anti-human immunodeficiency virus type 1 activity of APOBEC3G in resting primary CD4 T cells. J Virol. 2006, 80 (15): 7645-7657. 10.1128/JVI.00206-06.PubMed CentralView ArticlePubMedGoogle Scholar
- Towers G, Bock M, Martin S, Takeuchi Y, Stoye JP, Danos O: A conserved mechanism of retrovirus restriction in mammals. Proc Natl Acad Sci U S A. 2000, 97 (22): 12295-12299. 10.1073/pnas.200286297.PubMed CentralView ArticlePubMedGoogle Scholar
- Stoye JP: Fv1, the mouse retrovirus resistance gene. Rev Sci Tech. 1998, 17 (1): 269-277.PubMedGoogle Scholar
- Nisole S, Saïb A: Early steps of retrovirus replicative cycle. Retrovirology. 2004, 1 (1): 9-10.1186/1742-4690-1-9.PubMed CentralView ArticlePubMedGoogle Scholar
- Taylor MD, Korth MJ, Katze MG: Interferon treatment inhibits the replication of simian immunodeficiency virus at an early stage: evidence for a block between attachment and reverse transcription. Virology. 1998, 241 (1): 156-162. 10.1006/viro.1997.8964.View ArticlePubMedGoogle Scholar
- Korth MJ, Taylor MD, Katze MG: Interferon inhibits the replication of HIV-1, SIV, and SHIV chimeric viruses by distinct mechanisms. Virology. 1998, 247 (2): 265-273. 10.1006/viro.1998.9249.View ArticlePubMedGoogle Scholar
- Stark GR, Kerr IM, Williams BR, Silverman RH, Schreiber RD: How cells respond to interferons. Annu Rev Biochem. 1998, 67: 227-264. 10.1146/annurev.biochem.67.1.227.View ArticlePubMedGoogle Scholar
- Regad T, Chelbi-Alix MK: Role and fate of PML nuclear bodies in response to interferon and viral infections. Oncogene. 2001, 20 (49): 7274-7286. 10.1038/sj.onc.1204854.View ArticlePubMedGoogle Scholar
- Everett RD, Chelbi-Alix MK: PML and PML nuclear bodies: implications in antiviral defence. Biochimie. 2007, 89 (6-7): 819-830. 10.1016/j.biochi.2007.01.004.View ArticlePubMedGoogle Scholar
- Tissot C, Mechti N: Molecular cloning of a new interferon-induced factor that represses human immunodeficiency virus type 1 long terminal repeat expression. J Biol Chem. 1995, 270 (25): 14891-14898. 10.1074/jbc.270.25.14891.View ArticlePubMedGoogle Scholar
- Barr SD, Smiley JR, Bushman FD: The Interferon Response Inhibits HIV Particle Production by Induction of TRIM22. PLoS Pathog. 2008, 4 (2): e1000007-10.1371/journal.ppat.1000007.PubMed CentralView ArticlePubMedGoogle Scholar
- Gack MU, Shin YC, Joo CH, Urano T, Liang C, Sun L, Takeuchi O, Akira S, Chen Z, Inoue S, Jung JU: TRIM25 RING-finger E3 ubiquitin ligase is essential for RIG-I-mediated antiviral activity. Nature. 2007, 446 (7138): 916-920. 10.1038/nature05732.View ArticlePubMedGoogle Scholar
- Rajsbaum R, Stoye JP, O'Garra A: Type I interferon-dependent and -independent expression of tripartite motif proteins in immune cells. Eur J Immunol. 2008, 38 (3): 619-630. 10.1002/eji.200737916.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.