| FOLD RESTRICTION |
---|
 | HIV-1 | N-MLV | NB-MLV | B-MLV | SIVmac |
---|
MOI | 0.5 | 5 | 0.5 | 5 | 0.5 | 5 | 0.5 | 5 | 0.5 | 5 |
---|
MDTF
| 1.2 | 1.0 | 1.0 | 1.1 | 1.1 | 1.0 | 1.1 | 1.1 | 1.1 | 1.0 |
HeLa
| 1.5 | 1.1 |
3.7
|
7.6
| 1.9 | 1.4 | nt | nt | nt | nt |
CMMT
|
2.8
| 1.2 | 1.1 | 1.0 | 1.1 | 1.1 | nt | nt | nt | nt |
Vero
| 1.2 | 1.3 |
4.4
|
8.8
| 1.5 | 1.2 | nt | nt | nt | nt |
OMK
|
2.9
|
26.1
|
5.6
|
3.9
|
5.6
|
5.5
|
2.1
|
3.9
| 1.1 | 1.1 |
- HeLa, CMMT, Vero or OMK cells were stimulated with IFN-β and transduced 24 h later at a MOI of 0.5 or 5 (as determined on MDTF cells) with HIV-1, N-MLV, NB-MLV, B-MLV or SIVmac, as indicated. The same experiment was performed in parallel on MDTF cells treated or not with universal type I IFN. Fold restriction represents the ratio of untreated to IFN-treated cells to be transduced by the GFP-encoding retroviral vectors. A significant enhancement of retroviral restriction is defined as a ratio > 2 (in bold). Data are from a typical experiment representative of three independent experiments. nt: not tested.