Figure 6

The IFN-induced enhancement of anti-HIV-1 restriction in OMK cells is due to TRIMCyp. A. OMK cells were induced with IFN-β and challenged 24 h later with GFP-expressing HIV-1, N-MLV, B-MLV or NB-MLV at a MOI of 5. CsA (5 μM) was added 2 h before transduction. Percentage of transduced cells was determined by FACS 48 h post-transduction. Fold restriction represents the ratio of untreated to IFN-β-treated cells to be transduced by the GFP-encoding retroviral vectors. Results are representative of two independent experiments with comparable results. A significant enhancement of retroviral restriction is defined as a ratio > 2. B. OMK cells were stimulated with IFN-β and transduced 24 h later with VSV-pseudotyped GFP-expressing HIV-1, SIVmac or HIV-1 SCA vectors at a MOI of 1. The percentage of transduced cells was determined by FACS 48 h post-transduction. Fold restriction represents the ratio of untreated/IFN-β-treated cells to be transduced. As in panel A, a significant enhancement of retroviral restriction is defined as a ratio > 2.