- Open Access
Identification of low molecular weight nuclear complexes containing integrase during the early stages of HIV-1 infection
- Annabelle Gérard†1, 2, 3,
- Nicolas Soler†1, 2, 3,
- Emmanuel Ségéral1, 2, 3,
- Michael Belshan4, 5 and
- Stéphane Emiliani1, 2, 3Email author
© Gérard et al.; licensee BioMed Central Ltd. 2013
- Received: 29 June 2012
- Accepted: 3 January 2013
- Published: 1 February 2013
HIV-1 replication requires integration of its reverse transcribed viral cDNA into a host cell chromosome. The DNA cutting and joining reactions associated to this key step are catalyzed by the viral protein integrase (IN). In infected cells, IN binds the viral cDNA, together with viral and cellular proteins, to form large nucleoprotein complexes. However, the dynamics of IN complexes formation is still poorly understood.
Here, we characterized IN complexes during the early stages of T-lymphocyte infection. We found that following viral entry into the host cell, IN was rapidly targeted to proteasome-mediated degradation. Interactions between IN and cellular cofactors LEDGF/p75 and TNPO3 were detected as early as 6 h post-infection. Size exclusion chromatography of infected cell extracts revealed distinct IN complexes in vivo. While at 2 h post-infection the majority of IN eluted within a high molecular weight complex competent for integration (IN complex I), IN was also detected in a low molecular weight complex devoid of full-length viral cDNA (IN complex II, ~440 KDa). At 6 h post-infection the relative proportion of IN complex II increased. Inhibition of reverse transcription or integration did not alter the elution profile of IN complex II in infected cells. However, in cells depleted for LEDGF/p75 IN complex II shifted to a lower molecular weight complex (IN complex III, ~150 KDa) containing multimers of IN. Notably, cell fractionation experiments indicated that both IN complex II and III were exclusively nuclear. Finally, IN complex II was not detected in cells infected with a virus harboring a mutated IN defective for LEDGF/p75 interaction and tetramerization.
Our findings indicate that, shortly after viral entry, a significant portion of DNA–free IN that is distinct from active pre-integration complexes accumulates in the nucleus.
- Human immunodeficiency virus
- Pre-integration complex
During the early stages of retroviral replication, the virus travels from the cellular plasma membrane across the nuclear pore to finally integrate its viral cDNA into the host cell genome. These early events first require the reverse transcription of the viral RNA into a linear double strand cDNA copy by the viral reverse transcriptase (RT). Once synthesized, this cDNA becomes part of a large nucleoprotein complex, called the pre-integration complex (PIC) (reviewed in ). PICs from Moloney murine leukemia virus (MLV) [2–4] or Human immunodeficiency virus (HIV) [5–7] can be partially purified after cell infection and can efficiently integrate their associated reverse transcribed viral cDNA into heterologous DNA targets in vitro. The integration reaction is mediated by the retroviral integrase (IN) [9–11]. Within the PIC, IN binds to viral cDNA ends [12–14] and catalyzes the DNA cutting and joining reactions. First, the 3′ processing reaction consists in the hydrolysis of a dinucleotide at each end of the viral cDNA [4, 15, 16]. Then, exposed recessed 3′ hydroxyl groups of the viral cDNA are joined to the 5′ ends of the cut host target DNA [4–6, 15]. At this stage, cellular enzymes are probably in charge of removing the 5′ unpaired viral DNA ends and subsequently catalyze the gap filling and ligation reactions of host-viral DNA junctions . Human immunodeficiency virus type-1 (HIV-1) integration produces a 5 bp duplication of the DNA host sequence at each end of the integrated provirus .
Retroviral PICs are large nucleoprotein complexes that contain several viral and cellular proteins in addition to IN and viral cDNA. Biochemical studies indicate that HIV-1 PICs contain the viral nucleocapsid (NC), matrix (MA), Vpr and RT proteins [19–25]. In contrast with MLV [2, 26], HIV-1 PICs were shown to be devoid of CA [19–25]. In addition, cellular proteins including barrier to auto-integration factor (BAF), high mobility group protein HMGA, Ku and LEDGF/p75 have been found to associate with partially purified HIV-1 PICs [20, 27–29].
HIV and other lentiviruses have the ability to infect non-dividing cells, such as terminally differentiated macrophages. Therefore, this large viral nucleoprotein complex (>50 nm) must pass through the nuclear pore with the active participation of cellular factors involved in nucleo-cytoplasmic shuttling. Although several viral proteins within the HIV-1 PIC contain karyophilic signals (MA, Vpr and IN), their exact role during PIC translocation into the nucleus is still controversial . The central polypurine track (cPPT), a cis-acting sequence that forms a short triple stranded DNA structure (the central DNA Flap) during reverse transcription, is also implicated in the nuclear import of HIV PICs [31, 32]. Importantly, reports show that the HIV-1 capsid (CA) is the dominant viral determinant for HIV-1 infection of non-dividing cells, and the kinetic of dissociation of CA from the viral core appears to be a critical step in controlling nuclear import [33, 34].
Among the HIV-dependency factors involved in HIV-1 replication, TNPO3 was recently shown to be involved in a nuclear import and/or preintegration step [35–40]. TNPO3 is a karyopherin from the importin-ß family that mediates transport of serine/arginine rich (SR) proteins into the nucleus in a phosphorylation-dependent manner . Using yeast two-hybrid screenings, we identified TNPO3 as a binding partner of IN [36, 42]. Although a direct interaction between HIV-1 IN and TNPO3 has been clearly established [36, 43, 44], recent reports indicated that HIV CA is one of the viral determinants important for TNPO3 requirement [8, 38–40, 44, 45].
Once inside the nucleus, IN catalyzes viral cDNA integration into the genome of the host cell [46, 47]. HIV-1 integration occurs preferentially in transcription units (TUs) of transcriptionally active genes whereas CpG islands and promoter regions are disfavored. Importantly, the targeting of viral integration to specific regions of the host chromosome is under the control of LEDGF/p75 [48–50]. LEDGF/p75 is a key factor of HIV-1 integration that was identified as an IN interacting factor [51–53]. LEDGF/p75 is a cellular chromatin-associated protein presumably involved in transcriptional regulation of cellular genes [54–56]. LEDGF/p75 is tightly associated to chromatin, and the molecular basis of this interaction involves its conserved PWWP and AT-hook domains in the N-terminal region of the protein [57, 58]. LEDGF/p75 plays an important role in lentiviral cDNA integration, as demonstrated by mutagenesis [52, 59–61], over-expression of LEDGF/p75 IBD (Integrase Binding Domain) [62, 63] as well as RNAi and knock-out studies [49, 50, 62–65]. Structural studies revealed the roles of both the catalytic core domain dimeric interface and the N-terminal domain of IN for high affinity binding to IBD [60, 66, 67]. Albeit not strictly essential for replication, LEDGF/p75 tethers PIC-associated IN to chromatin to presumably stimulate its enzymatic activity at the site of integration [57, 58].
In this study, we explored at early times post infection the dynamics of interaction between IN and its cellular and viral partners. However, the detection of IN in infected cells remains technically challenging. We took advantage of a previously characterized virus carrying an active tagged-IN with the HA epitope at the C-terminus  to purify and characterize IN complexes in the context of infected lymphocytes. Our results shed light on the stability and distribution of IN during early steps of HIV-1 infection. We show that IN is rapidly degraded in a proteasome-dependent manner upon virus entry into the cell. Immunoprecipitation experiments allowed us to detect interactions between IN and its cofactors LEDGF/p75 and TNPO3 at 6 h post-infection (p.i.). Using size exclusion chromatography, we uncover that IN exists in at least two distinct complexes in infected cells: a high molecular weight complex that co-fractionates with viral cDNA and integration activity, and a low molecular weight complex devoid of viral cDNA that is found exclusively in the nucleus and depends on LEDGF/p75 expression.
HIV-1 integrase is rapidly degraded in a proteasome-dependent manner upon cell infection
Dynamic distribution of HIV-1 integrase complexes during infection
We then addressed the distribution of viral proteins early following infection. At 2 h p.i., IN-HA was detected in 2 complexes of different size and composition (Figure 3B). The high molecular weight complex (IN complex I), which was excluded from gel separation range (>1.3 MDa), was the most abundant one (fractions 15–18, Figure 3B). Viral proteins capsid (CA) and matrix (MA) were mostly found as free molecules (fractions 36–44, Figure 3B and C), except for a small proportion co-eluting with IN complex I (fractions 15–17 for CA and fraction 17–20 for MA, Figure 3B).
Although present in a minor proportion, low molecular weight (IN complex II) IN-HA complex peaked in fractions 23–25 (around 440 KDa). At 6 h p.i., IN-HA was still found in IN complex I, but shifted readily to IN complex II, while both CA and MA were only detected in smaller peaks (fraction 36–44) (Figure 3C). Noteworthy, IN-HA detection required longer exposure times at 6 h than at 2 h p.i., due to its rapid degradation upon cell infection. Together, our results indicate that shortly after infection, IN associates with at least two distinct complexes.
IN low molecular weight complexes are devoid of viral DNA and in vitroPIC activity
Accumulation of IN complex II does not require reverse transcription nor integration but depends on LEDGF/p75 expression
IN complexes II and III are exclusively nuclear
A class II IN mutant virus failed to accumulate in low molecular weight complexes
Studies of retroviral pre-integration complexes have been hampered by the limited amounts available from infected cells. In this study, we took advantage of an infectious HIV-1 viral clone carrying a C-terminus HA-tagged IN protein to explore IN low molecular weight complexes shortly after cell entry.
Our results show that IN undergoes rapid degradation by the proteasome shortly after its entry in the cytoplasm, whereas CA and MA remained stable. Previous published data showed that HIV-1 IN over-expressed in cells is actively degraded by the ubiquitin/proteasome system in both N-end rule dependent and independent pathways [52, 70, 71, 74–76]. Furthermore, the binding to LEDGF/p75 was shown to stabilize IN by preventing its degradation by the proteasome [52, 75]. However, we observed a similar decrease of levels of the WT IN and the Q168A mutant impaired for LEDGF/p75 interaction. This is likely to reflect that at early steps of infection the vast majority of IN entering the cell cytoplasm is not yet accessible for LEDGF/p75 binding.
In preliminary studies aiming at analyzing the impact of HIV-1 infection on cellular cofactors complexes using a Superdex 200 10/300 GL column, we noticed that IN eluted in a low molecular weight complex that was distinct from the large IN-containing complex detected within the void volume of the gel filtration column. This large complex also co-eluted with viral DNA and integration activity, indicative of the presence of active PICs in these fractions. This observation is consistent with previous characterizations of retroviral nucleoprotein complexes in infected cells using gel filtration chromatography [2, 6, 21, 25]. In contrast, the low molecular weight complex containing IN was devoid of viral cDNA and integrase activity. This complex was not observed in previous studies of HIV-1 PIC composition using gel filtration chromatography  or fractionation by sucrose gradient . However, this is not surprising as these analyses were restricted to the cytoplasmic fraction of infected cells, precluding the detection of complex II that we found to be exclusively in the nuclear fraction. IN complex II could be similar to the one that was previously isolated from the nucleus of cells stably expressing IN. In this report, tetramers of INs associated with LEDGF/p75 were found in a complex with an estimated mass around 400 KDa . Accordingly, we found that depletion of LEDGF/p75 lead to a shift towards a lower molecular weight complex (IN complex III) that peaks at ~150 KDa and contains tetramers of IN.
Non-exclusive hypothesis could explain the presence of IN in a low molecular weight complex distinct from the PIC. First, IN complex II could be the result of PIC destabilization. Indeed, the relative increase of the proportion of IN complex II at 6 h p.i. could reflect a dynamic shift of IN from active PICs to lower molecular weight complexes lacking full-length viral cDNA. Several mechanisms could account for the escape of IN from active PICs. Inefficient reverse transcription [78, 79], or degradation of incoming viral cDNA by cytosolic exonucleases like TREX1  could lead to the destabilization of PICs and the release of free IN. However, we did not observe a significant change in the distribution of IN complexes I and II upon inhibition of reverse transcription by the non-nucleoside reverse transcriptase inhibitor Nevirapine, challenging the hypothesis that IN complex II formation is the result of abortive reverse transcription complexes. Moreover, a catalytic inactive mutant IND116A accumulated in complex II suggesting that it is not the result of a post-integration event. Further experiments will be required to assess the potential role of cellular exonucleases in the release of low molecular weight IN complexes distinct from the PICs.
A second hypothesis posits that IN complex II might originate from IN molecules that are not associated with the PIC. IN is incorporated in virions as part of the Gag-Pol polyprotein precursor, and it is generally admitted that between 30 to 100 of IN molecules are packaged per retroviral particle . Although a precise quantification of IN incorporated into HIV-1 particles is still being awaited, it is likely that IN is present in large excess. Indeed, the functional intasome is formed by tetramer of IN tightly bound to the LTR ends of the newly synthesized viral cDNA [82, 83]. Thus, this apparent excess of DNA-free IN could lead to the formation of IN complex II. Future experiments will reveal whether IN low molecular weight complexes play an active role during HIV-1 replication.
We show that, shortly after the virus enters the cell, a significant portion of IN distinct from active PICs forms a low molecular weight complex in the nucleus that is dependent on LEDGF/p75.
Cells and viruses
293 T cells were grown in DMEM plus glutamine, antibiotics and 10& decomplemented-FCS (foetal calf serum) (GibcoBRL, Invitrogen). SupT1, TC3 and TL34 cells were grown in RPMI 1640 plus glutamine, antibiotics and 10& decomplemented-FCS (foetal calf serum) (GibcoBRL, Invitrogen). Virus stocks were generated by transfecting 293 T cells with either wild type or IN-HA tagged Lai molecular clone (HIV-1IN-HA) using Fugene 6 reagent (Roche). HIV-1INQ168A-HA and HIV-1IN D116A-HA were generated by site-directed mutagenesis using HIV-1IN-HA as a template. Mutations were confirmed by sequencing and subcloned back into the PflMI sites of the WT HIV-1IN-HA. HIV-1 Δenv Luc has been previously described . VSV-G pseudotyped viruses were produced by co-transfecting HIV-1IN-HA or HIV-1 Δenv Luc with plasmid pMD.G . Twenty-four hours post-transfection, cells were washed with PBS and supernatants were collected at 48 h and 72 h post-transfection. The supernatants were 0.45-μm-filtered and ultracentrifuged at 150,000 g for 1 h 30 minutes at 4°C. Virus pellets from 30 ml of supernatants were resuspended in 75 μl of PBS. An equal volume of RPMI 1640 medium (GIBCO, Invitrogen) supplemented with 10& fetal calf serum and antibiotics was added, and virus stocks were stored at −80°C. HIV-1 CAp24 antigen was quantified by ELISA (Innotests HIV Antigen mAb, Innogenetics, France).
Cell infection and cell extract preparation
SupT1 cells (2 × 108) were infected with 200 μg of CAp24 (corresponding to a multiplicity of infection-MOI- of 5, as quantified by real time PCR) of HIV-1 or HIV-1 IN-HA virus in a total volume of 500 μl for 2 h at 37°C. When indicated, 20 μM of MG-132 was added along the course of infection. Two hours later, cells were washed three times in 25 ml of PBS and resuspended in RPMI 1640 medium supplemented with 10& fetal calf serum and antibiotics at a final concentration of 1.5 × 106 cells/ml. At different time post infection cells were harvested, washed twice with 25 ml of PBS and lysed in 3 cell pellet volumes of lysis buffer (20 mM Tris–HCl pH 8.0, 0.3 M KCl, 5 mM MgCl2, 10& (v/v) glycerol, 0.1& tween 20, 1 mM PMSF and protease inhibitor cocktail from Sigma). Cell lysis was completed by two successive rounds of freeze-thaw, then incubated for 30 min at 4°C on rotating wheel. Two successive centrifugation steps at 16,000 g for 30 min at 4°C allowed complete removal of insoluble materials. The collected supernatant corresponding to soluble proteins within the cells was called whole cell extracts (WCE). Cytosolic and nuclear extracts were obtained by sequential cell fractionation. Cells were first washed in buffer A (20 mM HEPES-KOH pH 7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM PMSF and protease inhibitor cocktail from Sigma) and lysed 5 min on ice in 2 cell pellet volumes in buffer A and 0.1& (v/v) NP-40. After centrifugation of the cell lysate at 10,000 g for 5 min, the supernatant corresponding to the cytosolic extract (CE) was collected and the pellet was resuspended in 3 volumes of buffer B (20 mM HEPES-KOH pH 7.9, 1.5 mM MgCl2, 0.5 M NaCl, 25& (v/v) glycerol, 1 mM PMSF and protease inhibitor cocktail from Sigma). After 15 minutes incubation on ice, the nuclear extract (NE) was collected by centrifugation for 15 minutes at 14,000 g. CE and NE were stored at −80°C.
PIC activity assay
The assay is based on the quantification of integration events of viral cDNA into a parental vector (pTZ-19R)  with the following modifications. SupT1 cells were infected for 2 h with a VSV-G pseudotyped HIV-1IN-HA virus and WCE were prepared at 6 h p.i. as previously described. WCE (3 mg) were injected into Superdex 200 10/300 GL gel filtration column. Each fraction was collected and treated with RNase A (final concentration at 20 μg/ml) for 20 minutes at RT in a final volume of 250 μl. Integration reaction assay was performed at 37°C for 45 minutes by mixing each fraction with 1 μg of target plasmid pTZ-19R in integrase reaction buffer (INRB: 20 mM HEPES-KOH pH 7.4, 150 mM KCl, 1 mM MgCl2, 4& glycerol, and 1 mM DTT added just before starting the reaction) in a final volume of 350 μl. For each fraction, negative control was performed by omitting the target plasmid in the integration reaction assay. The reaction was stopped by adding 0.5& SDS, 8 mM EDTA and 0.5 mg/ml proteinase K for 1 h at 56°C. DNA was then extracted with phenol and phenol:chloroform:isoamylalcohol 25:24:1, using glycogen as carrier, and stored at −20°C. Integration events were quantified using a two-step PCR reaction. In the first round PCR, integrated HIV-1 sequences were amplified using the HIV-1 specific LM667 primer  together with primers annealing to opposite strand of pTZ-19R in tail-to-tail fashion. TZ2414 primer sequence is 5′- GTTGTTCCAGTTTG GAACAAGAGTC-3′. TZ2413 primer sequence is 5′- ACTCAACCCTATCTCGGTCTATTC-3′. To evidence background PCR signal, TZ2414 and TZ2413 primers were omitted from each integration reaction. We performed the second nested-PCR steps using conditions previously described . Results shown are indicated as relative units of specific signal (signal from PCR with target and with TZ2414/TZ2413 primers) minus unspecific signal (signal from PCR without target or without TZ2414/TZ2413 primers). The copy number of integrated DNA was determined in reference to a standard curve obtained by concomitant two steps amplification of serial dilution of the standard pNLX-HIVLTR vector. This construct was generated by amplifying the LTR region (n.t. 1–702, numbering based on NL4.3 sequence) and cloning into BamH1 and Pst1 restriction endonuclease sites of pTZ-19R.
Quantification of viral cDNA by real-time PCR
Prior to infection, viral stocks were treated 1 h at 37°C with 100 U per ml of DNAseI (Roche applied Science). SupT1 cells (6x106) were infected with viral doses corresponding to 6 μg of HIV-1IN-HA CAp24 antigen in 12-wells plates. At 2 h p.i., cells were washed twice in PBS. At 2 h, 4 h, 6 h, 8 h and 10 h p.i. cells were harvested, washed twice in PBS and DNA was extracted using the QIAamp Blood DNA Minikit (Qiagen). Quantifications of viral DNA were performed by real-time PCR using the LightCycler 480 system (Roche Applied Science). Primers, probes and PCR run conditions were described previously . The copy numbers of HIV-1 late reverse transcription product (LRT) and 2-LTR circles were determined using standard curves obtained by amplification of cloned DNA containing the matched sequences. The copy number of integrated HIV-1 DNA was determined in reference to a standard curve generated by concomitant two-stage PCR amplification of a serial dilution of the standard HeLa HIVR7-Neo cell DNA . Copy numbers of each viral form were normalized with the number of cells obtained by the quantification by PCR of the β-globin gene according to the manufacturer instructions (Roche Applied Science).
For co-immunoprecipitation experiments, 3 mg of WCE were mixed with 50 μl (50& slurry) of anti-HA affinity matrix (clone 3 F10, Roche Applied Science) supplemented with protease inhibitor cocktail and 1 mM PMSF for 3 h at 4°C on rotating wheel. Beads were washed three times with 15 volumes PBS-0.1& tween 20 for 5 minutes on a rotating wheel at 4°C. IN-HA complexes were directly resuspended in 1× loading sample buffer and boiled for 5 min.
Whole cell extracts were subjected to size exclusion chromatography at 4°C using an AKTA purifier system (GE Healthcare). Four mg of WCE were injected on a Superdex 200 10/300 GL column and 400 μl fractions were collected at a flow rate of 0.5 ml/min of WCE buffer. Proteins were precipitated over-night at 4°C in 10& trichloroacetic acid, washed twice in cold acetone and analyzed by Western blotting. Protein standards (Sigma) were fractionated under same conditions to estimate the size of the eluted protein complexes. When required, DNA was extracted from each fraction using the QIAamp Blood DNA Minikit (Qiagen).
Cross-linking reactions were performed on gel filtration fractions collected in 20 mM HEPES-KOH pH 7.2, 0.3 M KCl, 5 mM MgCl2, 10& glycerol, 0.1& tween 20, 1 mM PMSF and protease inhibitor cocktail (Sigma). Fractions 30–32, corresponding to part of IN complex III, were pooled and proteins were cross-linked with 0.25 mM to 2 mM of the cross-linker ethylene glycol bis-succinimidylsuccinate (EGS, freshly prepared) for 30 minutes at room temperature. The reaction was stopped by adding Tris–HCl pH 7.5 at a final concentration of 50 mM for 15 minutes at room temperature.
SDS-Page and Western-blotting
Proteins were separated by 4-12& gradient SDS-PAGE (Invitrogen), transferred onto nitrocellulose membranes, and revealed by Western-blotting using the following antibodies as indicated: anti-CAp24 (AIDS Research and Reference Reagent Program), anti-IN (Santa Cruz), anti-MA (Hybridolab), anti-HA-HRP (clone 3 F10, Roche Applied Science), LEDGF/p75 (BD Bioscience), TNPO3 (Abcam), and α-tubulin (Sigma-Aldrich).
We are grateful to Fabrizzio Mammano (Inserm U941, Paris, France) for the generous gift of the pBru IN-HA plasmid, to Eric Poeschla (Mayo Clinic, Rochester, Minnesota, USA) for the generous gift of the TC3 and TL34 cell lines, to Clarisse Berlioz-Torrent, Sarah Gallois-Montbrun and Lisa Hayden for critical reading of the manuscript, and to Jean-Luc Darlix and all members of the Berlioz-Emiliani laboratory for helpful discussions. The following reagent was obtained through the AIDS Research and Reference Reagent Program, Division of AIDS, NIAID, NIH: HIV-1SF2 p24 Antiserum. We thank the Genome and sequencing research facility from Institut Cochin.
This work was supported by grants from the Agence Nationale de la Recherche sur le Sida et les Hépatites (ANRS) and Agence Nationale de la Recherche (ANR) [ANR-06-RIB-021-02, ANR-09-BIOT-006-02] to SE. A.G. was recipient of postdoctoral fellowships from ANRS and SIDACTION. N.S. was recipient of a postdoctoral fellowship from ANRS.
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