Figure 4
Retroviral DNA and integration activity co-eluted with IN complex I. SupT1 cells were infected with HIV-1IN-HA pseudotyped with VSV-G to increase infectivity. WCE were prepared at 6 h p.i. and subjected to gel filtration. (A) Fractions were collected and analyzed by Western blotting using antibody against HA. (B) DNA was extracted from each fraction and late reverse transcription product (LRT) content was quantified by real time PCR. (C) In vitro PIC activity was assayed for each fraction (see Methods for details).