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Figure 8 | Retrovirology

Figure 8

From: Identification of low molecular weight nuclear complexes containing integrase during the early stages of HIV-1 infection

Figure 8

IN complex II and III are exclusively nuclear. Control SupT1 cells TC3 (A) and LEDGF/p75-knock-down SupT1 cells (TL34) (B) were infected with HIV-1IN-HA. Cytosolic and nuclear extracts (CE and NE, respectively) were prepared at 2 h p.i. and subjected to gel filtration on a Superdex 200HR 10/300 column. Fractions were collected and analyzed by Western blotting using antibodies against LEDGF/p75, or HA. Inputs represent 3,5& of WCE load. LEDGF/p75 served as an internal control for the nuclear fraction in (A).

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