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Figure 5 | Retrovirology

Figure 5

From: Identification of low molecular weight nuclear complexes containing integrase during the early stages of HIV-1 infection

Figure 5

Inhibition of reverse transcription does not affect the accumulation of IN complex II. (A) IN is rapidly degraded in presence of Nevirapine. SupT1 cells were infected with HIV-1IN-HA in absence or presence of 1 μM of Nevirapine. At indicated time post infection, cells were lysed and analyzed using Western blotting with antibodies against HA and CA. The inhibition of reverse transcription was monitored in cell extracts by the quantification of late reverse transcription products (LRT) by real time PCR. (B) and (C) SupT1 cells were infected with HIV-1IN-HA in absence or presence of 1 μM of Nevirapine. The inhibition of reverse transcription was monitored in cell extracts by the quantification of late reverse transcription products (LRT) by real time PCR (B). WCE were prepared at 2 h p.i. and subjected to gel filtration. Fractions were collected and analyzed by Western blotting using antibody against HA (C).

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