Infectious SIV resides in adipose tissue and induces metabolic defects in chronically infected rhesus macaques
© Couturier et al. 2016
Received: 2 March 2016
Accepted: 7 April 2016
Published: 27 April 2016
HIV reservoirs pose major challenges to viral eradication. The main cellular reservoirs include CD4 T cells and macrophages, whereas anatomic reservoirs are thought to be primarily lymphoid tissues. Adipose tissue represents a potentially important non-lymphoid location for HIV replication and persistence because the stromal-vascular-fraction (AT-SVF) contains activated innate and adaptive immune cells that increase in number during infections, obesity, and chronic inflammation.
Adipose tissue from two groups of SHIV-SF162p3-infected (~4 weeks acute infection) or SIVmac251-infected (~38 weeks chronic infection) rhesus macaques (N = 8 for each group) were studied for immune cell content, viral infectiousness, and metabolic health. The AT-SVF cells from SHIV-infected monkeys contained abundant memory CD4 and CD8 T cells, with fewer NKT cells and macrophages, and no B cells. Proviral DNA (Gag and Env) was readily detectable by nested PCR in AT-SVF cells from multiple adipose depots (subcutaneous and visceral) of acutely infected monkeys, but mostly from visceral fat. More importantly, viral outgrowth assays using input CD4 T cells derived from AT-SVF cells or peripheral blood of chronically infected monkeys resulted in robust replication of infectious virus from both AT-SVF and peripheral blood CD4 T cells. Chronically infected monkeys also experienced adipocyte dysfunction (suppression of major adipogenic genes) and systemic dyslipidemia (decreased serum total cholesterol and free fatty acids, and increased triglycerides), similar to metabolic abnormalities of HIV patients.
Adipose tissues of SIV-infected rhesus macaques become major compartments for infected immune cells, which in turn induce defects in adipose tissue metabolism.
KeywordsAdipose tissue CD4 T cells HIV latency HIV reservoirs Rhesus macaques SIV reservoirs
Eradication and immune control of HIV is difficult due to the establishment of reservoirs and anatomic sanctuaries [1, 2]. Memory CD4 T cells and macrophages are the primary hosts and cellular reservoirs for HIV in humans and SIV in non-human primates. Anatomic reservoirs for infected immune cells include lymphoid (lymph nodes, spleen, thymus, bone marrow, and GALT) and non-lymphoid (lungs, skin, liver, kidneys, reproductive, and nervous system) tissues. Antiretroviral therapy (ART) reduces the systemic viral load below the detection limits of clinical assays in most patients, but viral replication typically resumes if ART is interrupted . ART is also incompletely effective in certain locations, such as CNS tissue and lymph nodes, due to unique cellular barriers to ART drugs [4, 5]. Hence, understanding cellular and anatomic reservoirs for HIV, and how they may prevent adequate tissue penetration by ART drugs, is critical to achieving a cure.
A major organ that has been overlooked hitherto for a role in HIV pathogenesis is adipose tissue. It is well-known that a range of immune cells reside in or migrate into adipose tissues and affect their metabolic signals [6, 7], but adipocytes and other adipose tissue-resident cells interact with CD4 T cells and macrophages which has important implications for HIV pathogenesis. Anatomically, adipose tissue predominantly underlies the skin (subcutaneous fat) and surrounds abdominal organs (visceral fat). However, adipocytes are also intimately associated with most lymphoid tissues—lymph nodes are tightly encapsulated by adipose tissue, adipocytes are abundant within bone marrow, and the thymus gradually becomes filled with adipocytes during adult aging. Such an intricate arrangement is important during infections or immunological reactions because adipocytes are major sources of energy and survival signals for immune cells . At the cellular level, adipose tissue is heterogeneous and composed of mature adipocytes (the predominant fraction containing lipid droplets and triglycerides), and the stromal-vascular-fraction (AT-SVF) which includes mainly preadipocytes (adipocyte precursors) and fibroblasts, mesenchymal stem cells (MSC), endothelial cells, and immune cells. Extensive studies in humans and mice have demonstrated the presence of virtually every type of innate and adaptive leukocyte within adipose tissue during normal conditions, the composition and functions of which change dramatically in response to disease and inflammation. Importantly for HIV infection, the adipose tissue-resident CD4 T cells, which are predominantly activated memory CD4 T cells (CD45RO+CD69+HLA.DR+CD25+) [9–13], phenotypically resemble those in other tissues where HIV persists. We recently demonstrated for the first time the presence of memory CD4 T cells and HIV proviral DNA within the stromal-vascular-fraction of virally-suppressed ART-treated patients . In addition, we showed by in vitro co-culture experiments that primary human adipocytes enhance HIV replication in CD4 T cells . As adipocytes are ubiquitous endocrine cells that extensively regulate immunity and disease, these findings warrant further investigation into the role of adipose tissue in HIV replication and persistence.
SIV-infected rhesus macaques remain the best animal model for HIV infection and viral pathogenesis. In the present study, samples of adipose tissue were acquired from rhesus macaques at necropsy, which were infected with SHIV-SF162p3 for ~4 weeks (N = 8), or SIVmac251 for ~38 weeks (N = 8), and not treated with antiretroviral drugs. We hypothesized that memory CD4 T cells in adipose tissue harbors infectious virus, and that these untreated infected monkeys would develop metabolic complications similar to HIV-infected humans. Although the original purpose of these infected monkeys did not include the study of adipose tissue, examination of their fat tissue demonstrated that infiltration of adipose tissue by CD4 T cells infected with infectious virus is a regular event during SIV infection. Long-term infection of monkeys also resulted in some metabolic abnormalities resembling those of HIV patients. The present findings highlight the prevalence and stability of the viral reservoir in adipose tissue, and provide novel evidence for viral-induced metabolic dysfunction.
Establishment of reservoirs of memory CD4 T cells and SIV throughout adipose tissue during primary infection
Viral eradication is challenged by the rapidity with which SIV spreads throughout lymphoid tissues (within 7 days), and stable SIV reservoirs established (within 3 days) following infection of rhesus macaques [14, 15]. Adipose tissue inflammation and dysfunction typically involves the accumulation and regulatory activities of numerous innate and adaptive immune cells, particularly proinflammatory memory T cells, macrophages, and NKT cells. Thus, it is plausible that primary infection also includes viral dissemination and establishment of reservoirs in adipose tissue as adipocytes are intricately associated with most lymphoid tissues.
From total AT-SVF cells, CD3+ T cells accounted for ~9.8 % for uninfected healthy monkeys, ~6.1 % for SHIV-infected monkeys, and ~15.3 % for uninfected monkeys with chronic enterocolitis (Fig. 1b). Additionally, whereas CD8 and CD4 T cell levels were similar in AT-SVF of uninfected healthy monkeys (ratio of 1.38, p = 0.18), the ratio of AT-SVF CD8 to CD4 T cells increased during infection and disease (1.51 during SHIV infection, and 1.59 during chronic enterocolitis, p < 0.05) (Fig. 1c). For both uninfected healthy and SHIV-infected monkeys, nearly all of the AT-SVF CD4 and CD8 T cells were memory T cells (>94 % CD95+), which expressed tissue-resident and activation markers (62–84 % CD69+ and 3–13 % CD25+) (Fig. 1d). However, CD4 T cells expressed more CD25 (12–13 %) compared to CD8 T cells (3–4 %, p < 0.0001), possibly indicative of Treg subsets. AT-SVF cells of uninfected healthy and SHIV-infected monkeys were also comprised of similar levels of NKT cells (Fig. 1e), which were 3–8 % CD16+, 82–88 % CD27+, and 7–9 % CD56+ (gated on CD3+ cells), and 27–30 % GrzA+ and 49–61 % GrzB+ (gated on CD3+/CD56+ cells). Lastly, CD3-/CD14+/HLA.DR+ macrophages represented a small proportion (<1–2 %) of total AT-SVF cells of uninfected healthy and SHIV-infected monkeys (Fig. 1f), and CD3-/CD19+/CD80+ B cells were not present in AT-SVF cells (<0.8 %, data not shown). Thus, primary SIV infection is associated with homing of the major reservoir cell, memory CD4 T cells, into adipose tissue, which is further associated with minor changes of T cell and macrophage distributions.
To examine the SHIV-SF162p3 proviral content in AT-SVF cells of acutely infected monkeys, a nested PCR assay was utilized since AT-SVF cell numbers were limited and CD4 T cells comprised a small proportion (usually less than ~5 %) of total AT-SVF cells. Approximately 5 × 104–2 × 105 subcutaneous and visceral AT-SVF cell equivalents of DNA were used for each nested PCR reaction (6–9 replicates for each sample). For comparison, DNA extracted from other tissues including PBMC, mesenteric lymph nodes, and intestinal tissues were also examined (replicates of three for each sample). SHIV DNA (Gag and Env) was readily detectable in AT-SVF samples of all eight infected monkeys (but absent in tissues of the failed infection monkey), with more consistent detection in visceral AT-SVF samples compared to subcutaneous AT-SVF (Fig. 1g). Additionally, the 2nd round PCR bands of AT-SVF samples were gel-purified and sequenced, which showed that Gag and Env sequences were virtually identical amongst all monkeys (Additional file 2), consistent with previous reports demonstrating high sequence homology and minimal viral evolution in tissues during early infection periods of rhesus macaques [16, 17]. These data show that memory CD4 T cells and SIV reservoirs are widely distributed throughout adipose tissue of rhesus macaques during primary infection.
Infectious SIV harbored by adipose tissue CD4 T cells
Despite the efficacy of antiretroviral therapy to suppress viral replication in HIV patients, persistence of latently-infected CD4 T cells that harbor dormant, but replication-competent, provirus remains a major obstacle to eradication. These infected cells are unaffected by ART drugs and undetectable by antiviral CD8 T cells, and viral replication typically resumes within a month if therapy is discontinued. Whereas tissues such as lymph nodes containing CD4 T cells are important sources of rebound viremia, the contribution of other tissues such as the GALT is less clear [3, 18]. However, the presence of infectious virus in adipose tissue depots could also contribute to systemic viremia.
We additionally assessed if infectious virions may be sequestered or trapped by mature adipocytes in the adipocyte fraction of isolated adipose tissue samples, as it has been suggested that HIV may non-productively infect adipocytes . From three infected monkeys, ~6 × 106 M8166 cells were incubated with rotation at 37 °C in 5–8 ml of visceral floater fraction suspension from each monkey for 8 h, followed by centrifugation, washing, and removal of dead M8166 cells by density-gradient centrifugation. 3 × 106 M8166 cells were then cultured for up to 3 weeks and extracellular p27 measured, in which p27 was not detected (data not shown), indicating a lack of infectious virion sequestration or trapping by adipocytes.
Induction of metabolic perturbations by SIV infection in the absence of antiretroviral drugs
Metabolic dysfunction (such as dyslipidemias, hyperlipolysis, and decreased leptin and adiponectin production) and adipocyte abnormalities (such as differentiation block due to blunted expression of key adipogenic transcription factors) are prevalent during HIV infection. Whereas some of these defects have been attributed to the adverse effects of ART drugs, similar complications also occur in untreated or ART-naïve HIV patients. Additionally, viral proteins such as Vpr, Nef, and Tat impair adipocyte functions directly [20–24].
Consistent with the dysregulated expression of adipocyte factors, abnormal serum levels of important metabolic factors (total cholesterol, triglycerides, free fatty acids, leptin, and adiponectin) were observed in infected monkeys. When grouped by gender (eight acutely infected females, and four each chronically infected males and females) (since gender differences are well-known to influence metabolic parameters), and compared between baseline and necropsy time points, more changes of serum metabolites were observed in chronically infected monkeys. Bodyweights of acutely and chronically infected female monkeys were unchanged, but modestly increased for chronically infected males (Fig. 4c). Serum leptin levels were unchanged during acute infection, but modestly increased during chronic infection (Fig. 4d), whereas serum adiponectin levels were unchanged during acute and chronic infection (Fig. 4e). Serum total cholesterol was unchanged during acute infection, but decreased for chronically infected males (Fig. 4f). Serum triglycerides and free fatty acids were unchanged during acute infection, whereas triglycerides were increased and free fatty acids decreased for chronically infected females (Fig. 4g, h). Lastly, these alterations of adipocyte and serum metabolism by chronically infected monkeys were observed in conjunction with high systemic viral loads (Fig. 2a), increased peripheral blood CD8 T cells, and increased serum inflammatory cytokines (Additional file 4), although changes in other tissues affected by HIV infection likely impact metabolic homeostasis as well. These data suggest that adipose tissue and systemic metabolic homeostasis of rhesus macaques are disrupted by SIV infection, which resemble metabolic complications of HIV patients.
A more complete understanding of cellular and anatomic compartments for HIV is important to permit targeted efforts to eradicate latent HIV infection. The present study demonstrates that adipose tissue of SIV-infected rhesus macaques is a major site for memory CD4 T cells harboring infectious virus. In light of the extensive characterization of adipose tissue-immune system interactions in recent years, it is significant that this study demonstrated an abundance of infectious virus in adipose tissue. Adipocytes express numerous chemokines that recruit memory CD4 T cells and monocytes into adipose tissue, where T cells and macrophages differentiate into proinflammatory subsets during disease and inflammation. Additionally, adipose tissue macrophages, stromal-vascular-fraction cells, and mature adipocytes activate CD4 T cells via soluble factors and direct contact mechanisms, and furthermore promote T cell survival [25–27]. HIV replication by adipose tissue CD4 T cells and macrophages may also be regulated by TLR stimulation, as breaches of intestinal epithelia result in infiltration of microbial products into visceral fat . Furthermore, microbial pathogens such as M. tuberculosis can establish reservoirs in adipocytes , which may influence the pathogenesis of HIV co-infections in adipose tissue. Future investigations should focus on better understanding the mechanisms of antiviral immunity and efficacy of antiretroviral therapies in adipose tissue.
The rhesus macaques in the present study were infected with two different strains of virus (SHIV-SF162p3 or SIVmac251) for different time periods (~4 or ~38 weeks), and represented a range of viral loads, immunological parameters (CD4/CD8 cell counts and systemic cytokine levels), ages (2-16yo), and gender. Despite this biological range, notable was the ease and consistency in which memory CD4 T cells, SHIV provirus, and infectious SIV could be detected in the adipose tissue of all infected monkeys studied (Figs. 1, 2). This corroborates recent studies demonstrating HIV- or SIV-infected immune cells in both subcutaneous and visceral fat of infected humans and cynomolgus macaques examined [12, 13], further emphasizing the regularity of viral dissemination throughout adipose tissue. In these investigations, it has been essential to examine specifically the stromal-vascular-fraction of adipose tissue (following collagenase digestion to separate these cells from the mature adipocyte fraction), as mature adipocytes comprise the majority of adipose tissue, and only less than 5–10 % of total AT-SVF cells were CD4 T cells or macrophages. As shown in Fig. 1, the accumulation of infected immune cells in adipose tissue during acute infection was accompanied by minor changes in T cell and macrophage distribution, possibly due to the short time period of infection. Compared to uninfected healthy monkeys, the adipose tissue CD4:CD8 T cell ratio of infected monkeys was slightly increased (suggesting increased CD4 depletion or increased CD8 homing), whereas macrophages were decreased and NKT cells unchanged (although examination of additional M1/M2 macrophage markers, and NKT cell markers such as NKG2A may indicate more distinct changes of these subsets). These findings indicate that adipose tissue, particularly visceral fat, become a reservoir for SIV early during infection, and remain an infectious viral reservoir during longer-term infections.
The infectiousness of adipose tissue CD4 T cell SIV was mostly comparable to peripheral blood virus (Fig. 2), and such replication-competence suggests that adipose tissue infected CD4 T cells may contribute to systemic viremia or viral rebound. SIV replication was inducible from adipose tissue CD4 T cells of all seven chronically infected monkeys examined, and from as few as ~1 × 104 input AT-SVF CD4 T cells from most monkeys. This finding is also consistent with the report by Damouche et al., which demonstrated higher replication-competence by adipose tissue CD4 T cells compared to peripheral blood CD4 T cells in ART-treated HIV patients . This may be because CD4 T cells in adipose tissue, as well as in other extravascular tissues, are predominantly memory CD4 T cells and the most productive host for HIV, by contrast to peripheral blood which contains mostly resting memory CD4 T cells and more naïve T cells. However, in the presence of multi-functional proinflammatory CD8 T cells, which comprised the majority of CD3 T cells in adipose tissue, propagation of infectious virus was inhibited (Fig. 3). Additionally, NKT cell populations (CD3+/CD16+/CD27+/CD56+/GrzA+/GrzB+) were also observed in AT-SVF of infected monkeys (Fig. 1e), which may exert additional antiviral functions. The proinflammatory cytokine phenotypes of AT-SVF T cells of infected monkeys are also consistent with a range of models of adipose tissue inflammation which collectively depict that adipose tissue of healthy and lean humans and mice contain predominantly anti-inflammatory Tregs and Th2 cells, but shifts toward proinflammatory Th1 and Th17 cells during obesity and other diseases [10, 30–34]. Such changes in CD4 T cell differentiation may yield more susceptible targets for HIV, as Th1 and Th17 cells are more productive hosts for HIV compared to Tregs or Th2 cells [35–37]. It is unclear how viral replication levels by CD4 T cells and macrophages in adipose tissue compare to other tissues, and how well antiviral immunity suppresses replication, but the large mass of adipose tissue in the body suggests that it may be a large infectious reservoir.
Adipose tissue dysfunction is common amongst HIV patients, manifested by a range of clinical effects from dyslipidemia to lipoatrophy and fat “redistribution” [38, 39]. The etiology of these manifestations is multifactorial, including the adverse effects of ART drugs, direct effects of viral proteins such as Vpr and Nef, and indirect effects of inflammation secondary to immune activation within adipose depots [40, 41]. The present study of untreated acutely and chronically infected monkeys suggests that more metabolic changes occur as infection progresses over time. Additionally, interactions amongst activated immune cells and adipocytes in adipose tissue may promote inflammation that precedes the systemic metabolic changes. Whereas acutely infected monkeys mostly experienced metabolic stability, chronically infected monkeys experienced reduction of serum total cholesterol and increased triglycerides (Fig. 4f–h). Serum leptin was also increased in chronically infected monkeys (Fig. 4d), which for the males is consistent with their modest weight gain (Fig. 4c). This weight change may also be related to the increased densities of adipocytes and SVF cells observed in chronically SIV-infected (15 months) cynomolgus macaques . Leptin can also promote T cell survival , which could influence HIV persistence in adipose tissue. Quantification of gene expression of factors critical for adipocyte differentiation and function (PPARγ2, C/EBPα, C/EBPβ, leptin, adiponectin, and GLUT4) indicated that SIV infection decreased the expression of most of these genes (Fig. 4a), which may increase the risk for fat redistribution, lipoatrophy, or insulin resistance. Although metabolic changes in chronically infected monkeys occurred in conjunction with increased plasma cytokines (Additional file 4), factors derived from other tissues affected by viral infection, particularly lymphoid tissues proximal to adipose tissue, likely contribute to adipocyte dysfunction as well.
Some of these metabolic changes parallel adipose metabolic defects demonstrated in other animal models in the absence of ART drugs. For example, mouse models have shown that circulating Vpr induces dyslipidemias, increases energy expenditure, and suppresses adipogenic gene expression and adipocyte growth in vitro [20, 22]. Soluble Nef can also mediate dyslipidemias in SIV-infected rhesus macaques (infection for 2 months following 6 months of a proatherogenic diet of high cholesterol and saturated fats) by decreasing liver ABCA1 expression and impairing reverse cholesterol transport . These findings are further supported by in vitro studies demonstrating more directly the negative effects of Vpr, Nef, and Tat on adipocyte functions [22, 23, 43, 44]. Thus, in the absence of antiretroviral treatments, the metabolic changes in the infected monkeys of the present study provides further evidence that viral infection within the stromal-vascular-fraction of adipose depots and the resultant immunological alterations can perturb metabolism and adipose tissue homeostasis.
Immune-regulatory factors that promote T cell homing and HIV replication was also expressed by adipocytes (Fig. 4b). Expression of these factors has been previously demonstrated in human and murine adipocytes [45–47], but not in adipocytes of non-human primates. The common gamma-chain cytokines IL-2, IL-7, and IL-15 are major regulators of T cell survival and homeostatic stimulation, but in combination with proinflammatory cytokines such as IL-6 and TNFα, and with other adipocyte-secreted factors, can upregulate T cell activation and HIV replication [12, 48, 49]. Although these factors are generally upregulated in adipose tissue during infections and disease, substantial increases of immune cells were not observed in adipose tissue of acutely infected monkeys compared to uninfected monkeys as shown in Fig. 1, possibly due to the short time period of infection or the requirement for additional chemotactic signals from other stromal-vascular-fraction cells. Consistent with previous reports showing relatively high expression of CCL19 compared to other chemokines in human and murine adipocytes , we observed higher expression of CCL19 by adipocytes of infected monkeys compared to uninfected monkeys. During HIV infection, CCL19 and CCL21 enhance steps of viral post-integration latency in CD4 T cells , which in combination with other stimulatory agents in adipose tissue, may facilitate the complete replication cycle of HIV. Thus, adipocyte immunokines may contribute to the homing and survival of infected CD4 T cells and macrophages in adipose tissue, thus “seeding” the viral reservoir in this organ.
It is possible that the antiviral efficacy of ART drugs may be compromised in adipose tissue, since some of these drugs are lipophilic and can be sequestered within the relatively larger-sized adipocytes [52, 53]. Such sequestration might prevent the drugs from penetrating the stromal-vascular compartment harboring infected immune cells. In virally-suppressed ART-treated HIV patients, low-level viral replication in lymphoid tissue sanctuary sites, in association with inadequate penetration by ART drugs, has recently been reported as an important mechanism of viral persistence [4, 5]. Such sanctuary sites may exist in other tissues, and ongoing studies are investigating the penetration and efficacy of ART drugs in adipose tissue.
A better understanding of HIV reservoirs and anatomic sanctuaries is essential for treatment and eradication efforts. SIV-infected rhesus macaques represent the best animal model for HIV pathogenesis, and in the present study, fat tissues of infected monkeys are demonstrated to contain abundant memory CD4 T cells, highly infectious virus, and proinflammatory immune cells. SIV infection furthermore induced metabolic complications in the absence of antiretroviral drugs, some of which resembled metabolic conditions of HIV patients. Thus, this major endocrine organ represents an expansive location for SIV and HIV reservoirs that gradually becomes dysfunctional during viral infection.
Animals and infections
All animal experiments were approved by the Institutional Animal Care and Use Committee at the University of Texas MD Anderson Cancer Center and were carried out according to the provisions of the Animal Welfare Act, PHS Animal Welfare Policy, and the principles of the NIH Guide for the Care and Use of Laboratory Animals, and the policies and procedures of the University of Texas MD Anderson Cancer Center. Rhesus macaques (Macaca mulatta) of Indian origin were maintained in the specific pathogen-free breeding colony at the Michael Keeling Center for Comparative Medicine and Research of The University of Texas MD Anderson Cancer Center (Bastrop, Texas). The chamber size for the animals was 44′W × 88′H × 160′D. Monkeys were given water ad libitum, and fed a commercial monkey diet (Harlan). Additional enrichment was provided in the form of manipulanda, visual stimulation or auditory stimulation, and combinations thereof. Animals were monitored daily, including weekends and holidays. Anesthetics/analgesics were used to minimize any discomfort, distress, pain, and injury the animal might experience. Animals were euthanized with ketamine (11 mg/kg), followed by Beuthanasia (1 ml/10 lbs). If any animal was moribund, unresponsive to treatment, could not eat or drink, was severely sick, or had symptoms of SAIDS, it was euthanized as per guidelines. Animals were anesthetized during procedures to minimize discomfort.
For infections, monkeys were fasted for a minimum of 24 h prior to exposure. Monkeys were first anesthetized with 10 mg/kg of body weight ketamine intramuscularly and 0.5 mg/kg xylazine, then placed in a sternal position with the pelvis propped at 45°. Monkeys were infected by intra-rectal inoculation of 1000 TCID50 clonal stocks of SHIV-SF162p3 or SIVmac251 (NIH AIDS Reagent Program). The infected monkey was then returned to its cage and kept tilted at 45° until full recovery from anesthesia. RNA plasma viral loads were measured as previously described . The SHIV-SF162p3-infected monkeys were necropsied ~4 weeks post-infection, and SIVmac251-infected monkeys necropsied ~38 weeks post-infection, and are referred to as acutely or chronically infected monkeys, respectively, throughout the manuscript.
During the course of the study, adipose tissue samples were also acquired from rhesus macaques that were uninfected and mostly healthy, or uninfected but afflicted with chronic enterocolitis. These samples were utilized for comparisons to the infected monkeys (i.e. adipose tissue immune cell distribution or adipocyte gene expression studies) as indicated in the Results section. The demographic details of all the rhesus macaques used in the study are shown in Additional file 5.
Isolation of stromal-vascular-fraction (AT-SVF) cells from adipose tissue, and PBMC isolation from peripheral blood
During necropsy of monkeys, adipose tissue samples (~5–15 g) were harvested from abdominal subcutaneous and visceral regions and immediately processed for AT-SVF isolation. For AT-SVF isolation, ~1–3 g of adipose tissue at a time was minced with scissors, then digested with 1 mg/ml collagenase type II (Sigma) in 6 ml PBS in 15 ml conical tubes for 30–60 min (with rotation at 37 °C). The digest was then centrifuged to pellet the AT-SVF cells, whereas mature adipocytes (floater fraction) remained suspended due to lipid droplet buoyancy. The adipocytes were harvested and stored at −80 °C for real-time PCR analyses. The AT-SVF cells were then washed with PBS/2 % FBS, filtered through 70 µm mesh, and maintained in complete RPMI-1640 medium (10 % FBS, 2 mM l-glutamine, 0.1 mM MEM nonessential amino acids, 2 mM sodium pyruvate, 25 mM HEPES, and 1X antibiotic–antimycotic) at 37 °C/5 % CO2 prior to experiments. AT-SVF cell yields were usually ~2 × 105–8 × 105 cells per gram of adipose tissue.
PBMC were isolated from EDTA-anticoagulated whole blood by overlay onto Ficoll-Paque PLUS (GE Healthcare) and density-gradient centrifugation. PBMC were then washed and maintained in complete RPMI medium prior to experiments.
Antibodies for flow cytometry analyses were obtained from Biolegend, BD Biosciences, or eBioscience. For examination of PBMC and AT-SVF leukocytes, cells were washed with PBS/2 % FBS and stained with the following mabs: CD3-Pacblue, CD4-PerCPCy5.5, CD8-FITC, CD95-PE, CD25-PECy7, and CD69-APC for T cells; CD3-Pacblue, CD16-AF700, CD56-PE, GrzB-AF647, and either GrzA-PerCPCy5.5 or CD27-PerCPCy5.5 for NKT cells (intracellular GrzA and GrzB were stained using BD Biosciences Cytofix/Cytoperm solutions); CD19-PE and CD80-FITC for B cells; and CD14-Pacblue, CD16-PE, and HLA.DR-APCCy7 for macrophages. Isotype controls were used to assess non-specific binding, and compensation settings determined with single-color and fluorescence-minus-one (FMO) controls. Data was acquired with a Gallios Flow Cytometer and analyzed with Kaluza software (Beckman-Coulter).
For intracellular cytokine secretion (ICS) assays, PBMC or AT-SVF cells were stimulated with PMA/IO in the presence of GolgiPlug (BD Biosciences Leukocyte Activation Cocktail) for 5 h. Cells were washed with PBS/2 % FBS and stained with viability dye and surface mabs (CD3-PerCPCy5.5, CD8-FITC or CD8-APCCy7) for 30 min, followed by fixation/permeabilization with Cytofix/Cytoperm solution (BD Biosciences) for 30 min. Cells were then washed with Perm/Wash buffer and incubated with intracellular mabs (TNFα-PECy7, IL-2-AF700, IFNγ-PE, or IL-17A-PE) for 30 min. Cells were washed and analyzed by flow cytometry.
Nested PCR and DNA sequencing
DNA extraction was conducted using QIAamp DNA Micro Kit (QIAGEN). Nested PCR was performed for detection of SHIV-SF162p3 DNA (SIVmac239 Gag and HIV-1 Env) in cells of acutely infected monkeys, and primers are listed in Additional file 6. 1st round reactions included 40 μl PCR SuperMix (Life Technologies), 200–300 nM primers, 0.1 μg DNA, and 10 μl water; PCR conditions were one cycle at 94 °C (2 min), then 35–40 cycles at 94 °C (30 s), 55 °C (30 s), and 72 °C (55 s), then final extension at 72 °C (10 min). 2nd round reactions were 40 μl PCR SuperMix, 200–300 nM primers, 3 μ1 of 1st round product, and 10 μl water, and PCR conditions were one cycle at 94 °C (2 min), then 35–40 cycles at 94 °C (30 s), 55 °C (30 s), and 72 °C (30 s), then final extension at 72 °C (10 min). 2nd round PCR products were then gel-purified and sequenced by the Baylor College of Medicine DNA Sequencing Core (using ABI 3130XL Genetic Analyzer and BigDye Terminator). Multiple sequence alignments were conducted with Clustal Omega.
CD4 T cell purification and viral outgrowth assays
CD4 T cells were purified from PBMC or AT-SVF cells of infected rhesus macaques using bead-based positive selection kits (Stemcell Technologies), and purity assessed by flow cytometry. Induction and propagation of SIV replication was conducted using a previously described viral outgrowth method with minor modifications . Cells were serially diluted (twofold) six times and activated with 5 µg/ml PHA-L+50 ng/ml IL-2 in complete RPMI medium (3 ml) for 2 days. Medium was then replenished and PHA-L removed, followed by addition of 2 × 105 M8166 cells (NIH AIDS Reagent Program) for viral propagation. Cells were cultured for up to 3–4 weeks, in which medium was replenished every 3–4 days and cultures split every 6–7 days. Extracellular p27 was measured by sandwich ELISA kits (XpressBio).
Serum analyte measurements
Serum of infected monkeys was collected by centrifugation of anti-coagulated whole blood and storage at −80 °C. Serum cytokines were measured using the MILLIPLEX Non-Human Primate Immunology Multiplex Assay (EMD Millipore), and analyzed with a Bio-Plex 200 System (Bio-Rad). Serum total cholesterol was measured by Infinity Cholesterol Liquid Stable Reagent (Thermo Scientific), serum triglycerides were measured using Triglycerides Reagent kit (Thermo Scientific), and serum free fatty acids were measured using NEFA-HR(2) kit (Wako Diagnostics). Serum leptin was measured using Non-Human Primate Leptin competitive ELISA kit (NeoScientific), and serum adiponectin (Acrp30) was measured using LEGEND MAX sandwich ELISA kit (Biolegend).
Total RNA was extracted from the floater fraction adipocytes of collagenase-digested adipose tissue samples using RNeasy Lipid Tissue Mini Kit (QIAGEN). RNA was reverse-transcribed to cDNA, and SYBR Green real-time PCR performed using the ABI 7300 System (Applied Biosystems). Primers (Additional file 7) were designed using Primer Express and synthesized by Sigma Genosys. Reaction conditions were one cycle at 95 °C (10 min), followed by 40 cycles of 95 °C (30 s), 55 °C (60 s), and 72 °C (60 s), and ended with one cycle of 95 °C (60 s), 55 °C (30 s), and 95 °C (30 s). GAPDH was used as the calibrator for normalization of gene expression, and fold changes were calculated using 2−ΔΔCT formula.
Data were analyzed with GraphPad Prism or Microsoft Excel. Comparisons utilized two-tailed student’s t test (paired or unpaired as appropriate), and p values less than 0.05 were considered significant.
JC performed experiments, analyzed data, and wrote the manuscript. NA performed experiments and analyzed data. PNN, WBB, MAB, and KJS conducted non-human primate studies and provided tissue samples. AB and DEL supervised the project and approved the manuscript. All authors read and approved the final manuscript.
We thank the Baylor College of Medicine DNA Sequencing Core for sequencing, and the Mouse Metabolism Core for serum metabolite measurements.
The authors declare that they have no competing interests.
All animal experiments were approved by the Institutional Animal Care and Use Committee at the University of Texas MD Anderson Cancer Center and were carried out according to the provisions of the Animal Welfare Act, PHS Animal Welfare Policy, and the principles of the NIH Guide for the Care and Use of Laboratory Animals, and the policies and procedures of the University of Texas MD Anderson Cancer Center.
This research was funded by NIH/NIDDK Grant 1R01DK081553 (AB), NIH/NIAID Grant R21AI116208 (AB and DEL), and NIH/NIAID Baylor College of Medicine/University of Texas at Houston Center for AIDS Research (CFAR) Grant 5P30AI36211 (DEL).
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- Siliciano RF, Greene WC. HIV latency. Cold Spring Harb Perspect Med. 2011;1:a007096.View ArticlePubMedPubMed CentralGoogle Scholar
- Chun TW, Moir S, Fauci AS. HIV reservoirs as obstacles and opportunities for an HIV cure. Nat Immunol. 2015;16:584–9.View ArticlePubMedGoogle Scholar
- Rothenberger MK, Keele BF, Wietgrefe SW, Fletcher CV, Beilman GJ, Chipman JG, Khoruts A, Estes JD, Anderson J, Callisto SP, Schmidt TE, Thorkelson A, Reilly C, Perkey K, Reimann TG, Utay NS, Nganou Makamdop K, Stevenson M, Douek DC, Haase AT, Schacker TW. Large number of rebounding/founder HIV variants emerge from multifocal infection in lymphatic tissues after treatment interruption. Proc Natl Acad Sci USA. 2015;112:1126–34.View ArticleGoogle Scholar
- Fletcher CV, Staskus K, Wietgrefe SW, Rothenberger M, Reilly C, Chipman JG, Beilman GJ, Khoruts A, Thorkelson A, Schmidt TE, Anderson J, Perkey K, Stevenson M, Perelson AS, Douek DC, Haase AT, Schacker TW. Persistent HIV-1 replication is associated with lower antiretroviral drug concentrations in lymphatic tissues. Proc Natl Acad Sci USA. 2014;111:2307–12.View ArticlePubMedPubMed CentralGoogle Scholar
- Lorenzo-Redondo R, Fryer HR, Bedford T, Kim EY, Archer J, Kosakovsky Pond SL, Chung YS, Penugonda S, Chipman JG, Fletcher CV, Schacker TW, Malim MH, Rambaut A, Haase AT, McLean AR, Wolinsky SM. Persistent HIV-1 replication maintains the tissue reservoir during therapy. Nature. 2016;530:51–6.View ArticlePubMedGoogle Scholar
- Ferrante AW Jr. The immune cells in adipose tissue. Diabetes Obes Metab. 2013;15(Suppl 3):34–8.View ArticlePubMedPubMed CentralGoogle Scholar
- DiSpirito JR, Mathis D. Immunological contributions to adipose tissue homeostasis. Semin Immunol. 2015;27:315–21.View ArticlePubMedGoogle Scholar
- Pond CM. Paracrine relationships between adipose and lymphoid tissues: implications for the mechanism of HIV-associated adipose redistribution syndrome. Trends Immunol. 2003;24:13–8.View ArticlePubMedGoogle Scholar
- Duffaut C, Zakaroff-Girard A, Bourlier V, Decaunes P, Maumus M, Chiotasso P, Sengenès C, Lafontan M, Galitzky J, Bouloumié A. Interplay between human adipocytes and T lymphocytes in obesity: CCL20 as an adipochemokine and T lymphocytes as lipogenic modulators. Arterioscler Thromb Vasc Biol. 2009;29:1608–14.View ArticlePubMedGoogle Scholar
- Lysaght J, Allott EH, Donohoe CL, Howard JM, Pidgeon GP, Reynolds JV. T lymphocyte activation in visceral adipose tissue of patients with oesophageal adenocarcinoma. Br J Surg. 2011;98:964–74.View ArticlePubMedGoogle Scholar
- Travers RL, Motta AC, Betts JA, Bouloumié A, Thompson D. The impact of adiposity on adipose tissue-resident lymphocyte activation in humans. Int J Obes (Lond). 2015;39:762–9.View ArticleGoogle Scholar
- Couturier J, Suliburk JW, Brown JM, Luke DJ, Agarwal N, Yu X, Nguyen C, Iyer D, Kozinetz CA, Overbeek PA, Metzker ML, Balasubramanyam A, Lewis DE. Human adipose tissue as a reservoir for memory CD4 + T cells and HIV. AIDS. 2015;29:667–74.View ArticlePubMedPubMed CentralGoogle Scholar
- Damouche A, Lazure T, Avettand-Fènoël V, Huot N, Dejucq-Rainsford N, Satie AP, Mélard A, David L, Gommet C, Ghosn J, Noel N, Pourcher G, Martinez V, Benoist S, Béréziat V, Cosma A, Favier B, Vaslin B, Rouzioux C, Capeau J, Müller-Trutwin M, Dereuddre-Bosquet N, Le Grand R, Lambotte O, Bourgeois C. Adipose Tissue Is a Neglected Viral Reservoir and an Inflammatory Site during Chronic HIV and SIV Infection. PLoS Pathog. 2015;11:e1005153.View ArticlePubMedPubMed CentralGoogle Scholar
- Whitney JB, Hill AL, Sanisetty S, Penaloza-MacMaster P, Liu J, Shetty M, Parenteau L, Cabral C, Shields J, Blackmore S, Smith JY, Brinkman AL, Peter LE, Mathew SI, Smith KM, Borducchi EN, Rosenbloom DI, Lewis MG, Hattersley J, Li B, Hesselgesser J, Geleziunas R, Robb ML, Kim JH, Michael NL, Barouch DH. Rapid seeding of the viral reservoir prior to SIV viraemia in rhesus monkeys. Nature. 2014;512:74–7.PubMedPubMed CentralGoogle Scholar
- Ma ZM, Dutra J, Fritts L, Miller CJ. Lymphatic dissemination of SIV after penile inoculation. J Virol. 2016;90:4093–104View ArticlePubMedPubMed CentralGoogle Scholar
- Tsibris AM, Pal U, Schure AL, Veazey RS, Kunstman KJ, Henrich TJ, Klasse PJ, Wolinsky SM, Kuritzkes DR, Moore JP. SHIV-162P3 infection of rhesus macaques given maraviroc gel vaginally does not involve resistant viruses. PLoS One. 2011;6:e28047.View ArticlePubMedPubMed CentralGoogle Scholar
- Fieni F, Stone M, Ma ZM, Dutra J, Fritts L, Miller CJ. Viral RNA levels and env variants in semen and tissues of mature male rhesus macaques infected with SIV by penile inoculation. PLoS One. 2013;8:e76367.View ArticlePubMedPubMed CentralGoogle Scholar
- Lerner P, Guadalupe M, Donovan R, Hung J, Flamm J, Prindiville T, Sankaran-Walters S, Syvanen M, Wong JK, George MD, Dandekar S. The gut mucosal viral reservoir in HIV-infected patients is not the major source of rebound plasma viremia following interruption of highly active antiretroviral therapy. J Virol. 2011;85:4772–82.View ArticlePubMedPubMed CentralGoogle Scholar
- Munier S, Borjabad A, Lemaire M, Mariot V, Hazan U. In vitro infection of human primary adipose cells with HIV-1: a reassessment. AIDS. 2003;17:2537–9.View ArticlePubMedGoogle Scholar
- Balasubramanyam A, Mersmann H, Jahoor F, Phillips TM, Sekhar RV, Schubert U, Brar B, Iyer D, Smith EO, Takahashi H, Lu H, Anderson P, Kino T, Henklein P, Kopp JB. Effects of transgenic expression of HIV-1 Vpr on lipid and energy metabolism in mice. Am J Physiol Endocrinol Metab. 2007;292:E40–8.View ArticlePubMedGoogle Scholar
- Asztalos BF, Mujawar Z, Morrow MP, Grant A, Pushkarsky T, Wanke C, Shannon R, Geyer M, Kirchhoff F, Sviridov D, Fitzgerald ML, Bukrinsky M, Mansfield KG. Circulating Nef induces dyslipidemia in simian immunodeficiency virus-infected macaques by suppressing cholesterol efflux. J Infect Dis. 2010;202:614–23.View ArticlePubMedPubMed CentralGoogle Scholar
- Cheney L, Hou JC, Morrison S, Pessin J, Steigbigel RT. Nef inhibits glucose uptake in adipocytes and contributes to insulin resistance in human immunodeficiency virus type I infection. J Infect Dis. 2011;203:1824–31.View ArticlePubMedPubMed CentralGoogle Scholar
- Díaz-Delfín J, Domingo P, Wabitsch M, Giralt M, Villarroya F. HIV-1 Tat protein impairs adipogenesis and induces the expression and secretion of proinflammatory cytokines in human SGBS adipocytes. Antivir Ther. 2012;17:529–40.View ArticlePubMedGoogle Scholar
- Agarwal N, Iyer D, Patel SG, Sekhar RV, Phillips TM, Schubert U, Oplt T, Buras ED, Samson SL, Couturier J, Lewis DE, Rodriguez-Barradas MC, Jahoor F, Kino T, Kopp JB, Balasubramanyam A. HIV-1 Vpr induces adipose dysfunction in vivo through reciprocal effects on PPAR/GR co-regulation. Sci Transl Med. 2013;5:213ra164.View ArticlePubMedPubMed CentralGoogle Scholar
- Deng T, Lyon CJ, Minze LJ, Lin J, Zou J, Liu JZ, Ren Y, Yin Z, Hamilton DJ, Reardon PR, Sherman V, Wang HY, Phillips KJ, Webb P, Wong ST, Wang RF, Hsueh WA. Class II major histocompatibility complex plays an essential role in obesity-induced adipose inflammation. Cell Metab. 2013;17:411–22.View ArticlePubMedPubMed CentralGoogle Scholar
- Morris DL, Cho KW, Delproposto JL, Oatmen KE, Geletka LM, Martinez-Santibanez G, Singer K, Lumeng CN. Adipose tissue macrophages function as antigen-presenting cells and regulate adipose tissue CD4 + T cells in mice. Diabetes. 2013;62:2762–72.View ArticlePubMedPubMed CentralGoogle Scholar
- Cho KW, Morris DL, DelProposto JL, Geletka L, Zamarron B, Martinez-Santibanez G, Meyer KA, Singer K, O’Rourke RW, Lumeng CN. An MHC II-dependent activation loop between adipose tissue macrophages and CD4 + T cells controls obesity-induced inflammation. Cell Rep. 2014;9:605–17.View ArticlePubMedPubMed CentralGoogle Scholar
- Kruis T, Batra A, Siegmund B. Bacterial translocation—impact on the adipocyte compartment. Front Immunol. 2014;4:510.View ArticlePubMedPubMed CentralGoogle Scholar
- Neyrolles O, Hernández-Pando R, Pietri-Rouxel F, Fornès P, Tailleux L, Barrios Payán JA, Pivert E, Bordat Y, Aguilar D, Prévost MC, Petit C, Gicquel B. Is adipose tissue a place for Mycobacterium tuberculosis persistence? PLoS One. 2006;1:e43.View ArticlePubMedPubMed CentralGoogle Scholar
- Kintscher U, Hartge M, Hess K, Foryst-Ludwig A, Clemenz M, Wabitsch M, Fischer-Posovszky P, Barth TF, Dragun D, Skurk T, Hauner H, Blüher M, Unger T, Wolf AM, Knippschild U, Hombach V, Marx N. T-lymphocyte infiltration in visceral adipose tissue: a primary event in adipose tissue inflammation and the development of obesity-mediated insulin resistance. Arterioscler Thromb Vasc Biol. 2008;28:1304–10.View ArticlePubMedGoogle Scholar
- Strissel KJ, DeFuria J, Shaul ME, Bennett G, Greenberg AS, Obin MS. T-cell recruitment and Th1 polarization in adipose tissue during diet-induced obesity in C57BL/6 mice. Obesity (Silver Spring). 2010;18:1918–25.View ArticleGoogle Scholar
- Cipolletta D, Feuerer M, Li A, Kamei N, Lee J, Shoelson SE, Benoist C, Mathis D. PPAR-γ is a major driver of the accumulation and phenotype of adipose tissue Treg cells. Nature. 2012;486:549–53.PubMedPubMed CentralGoogle Scholar
- Fabbrini E, Cella M, McCartney SA, Fuchs A, Abumrad NA, Pietka TA, Chen Z, Finck BN, Han DH, Magkos F, Conte C, Bradley D, Fraterrigo G, Eagon JC, Patterson BW, Colonna M, Klein S. Association between specific adipose tissue CD4 + T-cell populations and insulin resistance in obese individuals. Gastroenterology. 2013;145:366–74.View ArticlePubMedPubMed CentralGoogle Scholar
- McLaughlin T, Liu LF, Lamendola C, Shen L, Morton J, Rivas H, Winer D, Tolentino L, Choi O, Zhang H, Hui Yen Chng M, Engleman E. T-cell profile in adipose tissue is associated with insulin resistance and systemic inflammation in humans. Arterioscler Thromb Vasc Biol. 2014;34:2637–43.View ArticlePubMedPubMed CentralGoogle Scholar
- Bahbouhi B, Landay A, Al-Harthi L. Dynamics of cytokine expression in HIV productively infected primary CD4 + T cells. Blood. 2004;103:4581–7.View ArticlePubMedGoogle Scholar
- El Hed A, Khaitan A, Kozhaya L, Manel N, Daskalakis D, Borkowsky W, Valentine F, Littman DR, Unutmaz D. Susceptibility of human Th17 cells to human immunodeficiency virus and their perturbation during infection. J Infect Dis. 2010;201:843–54.View ArticlePubMedPubMed CentralGoogle Scholar
- Chevalier MF, Weiss L. The split personality of regulatory T cells in HIV infection. Blood. 2013;121:29–37.View ArticlePubMedGoogle Scholar
- Koutkia P, Grinspoon S. HIV-associated lipodystrophy: pathogenesis, prognosis, treatment, and controversies. Annu Rev Med. 2004;55:303–17.View ArticlePubMedGoogle Scholar
- Falutz J. Therapy insight: Body-shape changes and metabolic complications associated with HIV and highly active antiretroviral therapy. Nat Clin Pract Endocrinol Metab. 2007;3:651–61.View ArticlePubMedGoogle Scholar
- Giralt M, Domingo P, Villarroya F. Adipose tissue biology and HIV-infection. Best Pract Res Clin Endocrinol Metab. 2011;25:487–99.View ArticlePubMedGoogle Scholar
- Koethe JR, Hulgan T, Niswender K. Adipose tissue and immune function: a review of evidence relevant to HIV infection. J Infect Dis. 2013;208:1194–201.View ArticlePubMedPubMed CentralGoogle Scholar
- Galgani M, Procaccini C, De Rosa V, Carbone F, Chieffi P, La Cava A, Matarese G. Leptin modulates the survival of autoreactive CD4 + T cells through the nutrient/energy-sensing mammalian target of rapamycin signaling pathway. J Immunol. 2010;185:7474–9.View ArticlePubMedGoogle Scholar
- Otake K, Omoto S, Yamamoto T, Okuyama H, Okada H, Okada N, Kawai M, Saksena NK, Fujii YR. HIV-1 Nef protein in the nucleus influences adipogenesis as well as viral transcription through the peroxisome proliferator-activated receptors. AIDS. 2004;18:189–98.View ArticlePubMedGoogle Scholar
- Shrivastav S, Kino T, Cunningham T, Ichijo T, Schubert U, Heinklein P, Chrousos GP, Kopp JB. Human immunodeficiency virus (HIV)-1 viral protein R suppresses transcriptional activity of peroxisome proliferator-activated receptor gamma and inhibits adipocyte differentiation: implications for HIV-associated lipodystrophy. Mol Endocrinol. 2008;22:234–47.View ArticlePubMedPubMed CentralGoogle Scholar
- Meijer K, de Vries M, Al-Lahham S, Bruinenberg M, Weening D, Dijkstra M, Kloosterhuis N, van der Leij RJ, van der Want H, Kroesen BJ, Vonk R, Rezaee F. Human primary adipocytes exhibit immune cell function: adipocytes prime inflammation independent of macrophages. PLoS One. 2011;6:e17154.View ArticlePubMedPubMed CentralGoogle Scholar
- Liou YH, Wang SW, Chang CL, Huang PL, Hou MS, Lai YG, Lee GA, Jiang ST, Tsai CY, Liao NS. Adipocyte IL-15 regulates local and systemic NK cell development. J Immunol. 2014;193:1747–58.View ArticlePubMedGoogle Scholar
- Pierce JR, Maples JM, Hickner RC. IL-15 concentrations in skeletal muscle and subcutaneous adipose tissue in lean and obese humans: local effects of IL-15 on adipose tissue lipolysis. Am J Physiol Endocrinol Metab. 2015;308:E1131–9.View ArticlePubMedGoogle Scholar
- Chun TW, Engel D, Mizell SB, Ehler LA, Fauci AS. Induction of HIV-1 replication in latently infected CD4 + T cells using a combination of cytokines. J Exp Med. 1998;188:83–91.View ArticlePubMedPubMed CentralGoogle Scholar
- Unutmaz D, KewalRamani VN, Marmon S, Littman DR. Cytokine signals are sufficient for HIV-1 infection of resting human T lymphocytes. J Exp Med. 1999;189:1735–46.View ArticlePubMedPubMed CentralGoogle Scholar
- Tourniaire F, Romier-Crouzet B, Lee JH, Marcotorchino J, Gouranton E, Salles J, Malezet C, Astier J, Darmon P, Blouin E, Walrand S, Ye J, Landrier JF. Chemokine expression in inflamed adipose tissue is mainly mediated by NF-κB. PLoS One. 2013;8:e66515.View ArticlePubMedPubMed CentralGoogle Scholar
- Saleh S, Solomon A, Wightman F, Xhilaga M, Cameron PU, Lewin SR. CCR7 ligands CCL19 and CCL21 increase permissiveness of resting memory CD4 + T cells to HIV-1 infection: a novel model of HIV-1 latency. Blood. 2007;110:4161–4.View ArticlePubMedGoogle Scholar
- Dupin N, Buffet M, Marcelin AG, Lamotte C, Gorin I, Ait-Arkoub Z, Tréluyer JM, Bui P, Calvez V, Peytavin G. HIV and antiretroviral drug distribution in plasma and fat tissue of HIV-infected patients with lipodystrophy. AIDS. 2002;16:2419–24.View ArticlePubMedGoogle Scholar
- Vernochet C, Azoulay S, Duval D, Guedj R, Cottrez F, Vidal H, Ailhaud G, Dani C. Human immunodeficiency virus protease inhibitors accumulate into cultured human adipocytes and alter expression of adipocytokines. J Biol Chem. 2005;280:2238–43.View ArticlePubMedGoogle Scholar
- Cline AN, Bess JW, Piatak M Jr, Lifson JD. Highly sensitive SIV plasma viral load assay: practical considerations, realistic performance expectations, and application to reverse engineering of vaccines for AIDS. J Med Primatol. 2005;34:303–12.View ArticlePubMedGoogle Scholar
- Shen A, Yang HC, Zhou Y, Chase AJ, Boyer JD, Zhang H, Margolick JB, Zink MC, Clements JE, Siliciano RF. Novel pathway for induction of latent virus from resting CD4(+) T cells in the simian immunodeficiency virus/macaque model of human immunodeficiency virus type 1 latency. J Virol. 2007;81:1660–70.View ArticlePubMedPubMed CentralGoogle Scholar