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Fig. 1 | Retrovirology

Fig. 1

From: Infectious SIV resides in adipose tissue and induces metabolic defects in chronically infected rhesus macaques

Fig. 1

Leukocyte and proviral distribution in the adipose tissue stromal-vascular-fraction (AT-SVF) of acutely infected monkeys. Visceral and subcutaneous adipose tissue samples were harvested from rhesus macaques at necropsy. AT-SVF cells were isolated as described in “Methods” section and examined for leukocyte content (T cells, NKT cells, and macrophages) by flow cytometry, and SHIV DNA detection (Gag and Env) by nested PCR. a Plasma viral loads of SHIV-SF162p3-infected rhesus macaques (infection was successful for eight out of nine monkeys). b, c Mean ± SEM percentages of AT-SVF CD3+, or CD4 (CD3+CD4+) and CD8 (CD3+CD4−) T cells of uninfected healthy and SHIV-infected monkeys (*p < 0.05). For additional comparisons, AT-SVF T cells of uninfected rhesus macaques with chronic enterocolitis were also examined. df Mean ± SEM percentages of AT-SVF T cell memory (CD95) and activation (CD25 and CD69), NKT cell (CD16, CD27, CD56, GrzA, and GrzB), and macrophage (CD14 and HLA.DR) markers of uninfected healthy or SHIV-infected monkeys (*p < 0.05). g Nested PCR detection (2nd round gel bands) of SHIV-SF162p3 Gag and Env genes in DNA extracted from subcutaneous and visceral AT-SVF cells (~5 × 104–2 × 105 cell equivalents of DNA) of infected monkeys. DNA from PBMC, mesenteric lymph nodes, and intestinal tissue were also examined for comparison. PCR replicates of 6–9 were tested for AT-SVF cells, and replicates of three were tested for PBMC, MLN, and intestinal tissues

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