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Fig. 3 | Retrovirology

Fig. 3

From: Infectious SIV resides in adipose tissue and induces metabolic defects in chronically infected rhesus macaques

Fig. 3

Multi-functionality of CD8 T cells in adipose tissue of infected rhesus macaques. a Lack of viral outgrowth from total AT-SVF cells (without CD8 T cell depletion) of three acutely SHIV-SF162p3-infected monkeys. Shown are input numbers of total AT-SVF cells at the start of the assay, and the ratio of AT-SVF CD8 to CD4 T cells. b, c Proinflammatory cytokine functionality of peripheral blood and AT-SVF T cells of chronically infected monkeys. Isolated PBMC or AT-SVF cells of SIVmac251-infected monkeys were untreated (UT) or stimulated with PMA/IO (in the presence of brefeldin) for 5 h. Cells were then stained for CD3, CD8, TNFa, IL-2, IFNγ, and IL-17A and analyzed by flow cytometry. Shown are representative cytokine dotplots gated on peripheral blood or AT-SVF CD3+/CD8+ or CD3+/CD8− T cells after PMA/IO activation, and mean ± SEM cytokine expression (N = 4–5)

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