- Open Access
Cerebellum-specific and age-dependent expression of an endogenous retrovirus with intact coding potential
© Lee et al; licensee BioMed Central Ltd. 2011
- Received: 25 April 2011
- Accepted: 12 October 2011
- Published: 12 October 2011
Endogenous retroviruses (ERVs), including murine leukemia virus (MuLV) type-ERVs (MuLV-ERVs), are presumed to occupy ~10% of the mouse genome. In this study, following the identification of a full-length MuLV-ERV by in silico survey of the C57BL/6J mouse genome, its distribution in different mouse strains and expression characteristics were investigated.
Application of a set of ERV mining protocols identified a MuLV-ERV locus with full coding potential on chromosome 8 (named ERVmch8). It appears that ERVmch8 shares the same genomic locus with a replication-incompetent MuLV-ERV, called Emv2; however, it was not confirmed due to a lack of relevant annotation and Emv2 sequence information. The ERVmch8 sequence was more prevalent in laboratory strains compared to wild-derived strains. Among 16 different tissues of ~12 week-old female C57BL/6J mice, brain homogenate was the only tissue with evident expression of ERVmch8. Further ERVmch8 expression analysis in six different brain compartments and four peripheral neuronal tissues of C57BL/6J mice revealed no significant expression except for the cerebellum in which the ERVmch8 locus' low methylation status was unique compared to the other brain compartments. The ERVmch8 locus was found to be surrounded by genes associated with neuronal development and/or inflammation. Interestingly, cerebellum-specific ERVmch8 expression was age-dependent with almost no expression at 2 weeks and a plateau at 6 weeks.
The ecotropic ERVmch8 locus on the C57BL/6J mouse genome was relatively undermethylated in the cerebellum, and its expression was cerebellum-specific and age-dependent.
- Olfactory Bulb
- Mouse Genome
- Protein Code Sequence
- Minus Strand
- Mouse Genome Database
The concept of "endogenous" retroviruses (ERVs), which are inherited to subsequent generations by Mendelian order, was introduced following the discovery of three variants of ERVs in the genomes of laboratory mice and domestic fowls: murine leukemia virus (MuLV), mouse mammary tumor virus (MMTV), and avian leukosis virus [1, 2]. ERVs are a family of long-terminal repeat (LTR) retrotransposons, and they occupy ~10% of the mouse genome [3, 4]. In conjunction with the ERV population data accumulated from studies during the last few decades, the current mouse genome database renders an in-depth and systematic cataloguing of ERVs and other transposable and/or repetitive elements [4, 5]. Mouse ERVs are segregated into three different classes (class I, II, III) based on the phylogenetic relatedness of their reverse transcriptase codons . Class I (e.g., MuLV-type ERVs [MuLV-ERVs]), class II (e.g., MMTV-type ERVs), and class III ERVs represent ~0.7%, ~3%, and ~5.4% of the mouse genome, respectively.
Some studies have shed an initial light into the biological properties of mouse ERVs. Rowe et al. reported that activation of recombinant MuLV-ERVs is linked to the onset of thymic lymphomagenesis . In addition, it has been demonstrated that extended culturing of embryonic cells derived from certain mouse strains, such as AKR mice, resulted in the de novo production and release of MuLV-type ERVs [8, 9]. Recent studies have suggested that the envelope gene products of ERVs participate in various pathophysiologic processes, such as placental morphogenesis in mice and demyelination of oligodendrocytes in multiple sclerosis patients [10, 11]. Our laboratory reported that stress signals elicited from injury and/or infection activate certain ERVs, and lipopolysaccharide treatment differentially induces the production and release of ERV virions from mouse primary lymphocytes of various origins and at different developmental stages [12–14]. Furthermore, it was observed that ERV expression patterns in mice are directly linked to ERV-, cell-, and/or tissue-type [14, 15].
In this study, using a combination of different ERV mining protocols, a full-length MuLV-ERV locus with an intact coding potential was identified from the C57BL/6J mouse genome. The genomic distribution of this ERV in different mouse strains and its expression characteristics in various tissues, including different brain compartments, were investigated.
Identification of a full-length MuLV-ERV locus on chromosome 8 of the C57BL/6J mouse genome
Distribution of the ERVmch8 sequence in the genomes of laboratory and wild-derived mouse strains
Brain-specific ERVmch8 expression
Age-dependent regulation of the expression of ERVmch8 in the cerebellum
Protein coding sequences neighboring the ERVmch8 locus
Unique methylation profile of the ERVmch8 locus in the cerebellum in comparison to the other brain compartments
Most characterized members of the C57BL/6J ERV population exist as multiple copies in the genome. A survey in this study identified only a single copy of an ecotropic ERV (ERVmch8) in the C57BL/6J mouse genome, and it is not currently annotated in the NCBI database (Build 37.1, as of November 12, 2010). ERVmch8 (8,728 nucleotides) shares a greater than 98% nucleotide sequence homology with the melanoma-associated retrovirus (MelARV), which was localized on chromosome 7 of the B16 melanoma cell line derived from the C57BL/6 mouse strain . According to the results obtained from the env polypeptide alignment against MelARV, it appears that ERVmch8 harbors an ecotropic tropism trait. Pothlichet et al. reported that a single locus on chromosome 8-qE1 was mapped using the MelARV env sequence as a query and presumed that MelARV originated from the Emv2 locus, which is reported to be the only ecotropic ERV found in normal C57BL/6 cells [17, 34]. Contrary to this report, the ERVmch8 locus, which has ~98% nucleotide sequence homology with MelARV, was mapped on the chromosome 8-qE1 junction, based on survey results using NCBI BLAST. In addition, Emv2 is located/annotated at 67.0 cM, ~11.4 cM upstream of the ERVmch8 locus (~78.4 cM), according to a survey of the NCBI map viewer [21, 35] http://www.ncbi.nlm.nih.gov/projects/mapview. Thus, it is probable that ERVmch8, but not Emv2, is the probable progenitor of MelARV, if any. Unexpectedly, we were unable to retrieve the nucleotide sequence, which is presumed to be the Emv2 provirus, from the Emv2 locus annotated in the NCBI C57BL/6J mouse genome (Build 37.1, as of November 12, 2010) and MGI (MGI_4.4 as of November 19, 2010) databases. Further, we were unsuccessful in locating the Emv2 proviral sequence, either partial or full, using the keyword, "Emv2", in the NCBI Nucleotide database. However, it is still a possibility that ERVmch8 shares the same locus on chromosome 8-qE1 region with Emv2 with an assumption that the NCBI annotation information regarding the Emv2 locus needs to be revised.
Analysis of the distribution of the ERVmch8 sequence among various mouse strains demonstrated that a majority of strains in Groups 1 and 4 of the phylogenetic tree, which was developed by Petkov et al., harbor the proviral sequence in their genome. Within Group 1, which consists of mostly laboratory strains, including BALB/cJ and C3H, all except for the SF/CamEiJ and CE/J strains had evident amplification of the ERVmch8 sequence. The C57L/J strain in Group 4, which also contains the C57BL/6J strain, did not have the ERVmch8 sequence amplified, and this finding is consistent with the description from the Jackson Laboratory that "C57L/J mice carry no detectable endogenous ecotropic MuLV DNA sequences". On the contrary, there was no amplification of the ERVmch8 sequence in the vast majority (16 of 19) of Group 7, which is comprised of wild-derived strains. Interestingly, a unique branch of three strains (MOLC/RkJ, MOLD/RkJ, and MOLF/EiJ) in Group 7, which had the ERVmch8 sequence amplified, were derived by independent pairings of Mus musculus molossinus mice originating from Fukuoka, Japan (JAX® NOTES Issue 456 and JAX Mice Database, Jackson Laboratory). The SPRET/EiJ mice, also from Group 7 and derived from wild mice caught in Puerto Real, Spain (JAX Mice Database, Jackson Laboratory), had no ERVmch8 sequence amplified. These findings suggest that the ERVmch8 sequence is present in wild mice originating only from certain geographic regions.
The unique methylation profile, in particular, the high number of converted cytosines in a segment of the ERVmch8 sequence of the cerebellum (~12 week-old mice) in comparison to the other brain compartments, may explain, at least in part, the cerebellum-specific expression of the ERVmch8 locus. Active transcription of this full-length MuLV-ERV (ERVmch8), presumed to retain the ecotropic tropism trait, from the age of five to six weeks may lead to a series of potential short-term and long-term events: 1) persistent expression of gag, pol, and env polypeptides, and their potential contribution to the biology of the cerebellum, 2) assembly of virus particles with ecotropic tropism followed by their release, and 3) very low-level, if any, infection (due to presumed to be poor replication-competency) of neighboring and/or distant cells expressing relevant receptor(s) during the course of the relatively long lifespan of brain cells .
The key finding of this study that ERVmch8 expression is cerebellum-specific and age-dependent suggests that the expression of ERVmch8 is linked to the biology of the cerebellum. A set of further experiments is needed to unveil the detailed mechanisms controlling the cerebellum-specific and age-dependent expression of ERVmch8. In addition, a full investigation into the roles of ERVmch8 in the biology of the cerebellum and potentially other tissues is warranted.
Eight different age groups (~2 to ~29 weeks) of female C57BL/6J mice and ~12 week-old females were purchased from the Jackson Laboratory-West (West Sacramento, CA). The experimental protocol was approved by the Animal Use and Care Administrative Advisory Committee of the University of California, Davis. Three mice from each age group were sacrificed by CO2 inhalation or cervical dislocation followed by harvesting of different sets of tissues depending on the age groups. Certain brain samples were dissected further into their separate compartments and all tissue samples were snap-frozen.
Genomic DNA samples from 57 different inbred mouse strains (both laboratory and wild-derived) were purchased from the Jackson Laboratory (Bar Harbor, Maine). Genotyping PCR was performed using 100 ng of genomic DNA to determine the presence of ERVmch8 sequence using Taq polymerase from Qiagen (Valencia, CA) and a set of primers; UM1: 5'-GAA GTT GAA AAG TCC ATC ACT AA-3' and UM3: 5'-TCT GGG TCT CTT GAA ACT GT-3'.
RNA isolation and RT-PCR
Total RNA was isolated from the tissue samples using an RNeasy Lipid Tissue Mini Kit (for brain tissues) or RNeasy Mini Kit (for non-brain tissues) from Qiagen. cDNAs were synthesized from 100 ng of total RNA using a QuantiTect Reverse Transcription Kit (Qiagen). A region near the 3'-end of the ERVmch8 transcript was amplified by PCR using the UM1 and UM3 primer set (see above). β-actin served as a normalization control. Primers for β-actin amplification are as follows; Forward: 5'-CCA ACT GGG ACG TGG AA-3' and Reverse: 5'-GTA GAT GGG CAC AGT GTG GG-3'.
Genomic DNA isolation, bisulfite treatment, and PCR amplification
Genomic DNA was isolated from six different brain compartments (cerebral cortex, corpus callosum, brain stem, cerebellum, hippocampus, and olfactory bulb) of ~12 weeks-old mice using a DNeasy Tissue Kit (Qiagen). For the conversion of unmethylated cytosines to uracils/thymines, 2 μg of genomic DNA from each sample was treated with bisulfite using a Methyl Detector Kit (Active Motif, Carlsbad, CA). PCR was performed using Taq polymerase (Qiagen), 7.5 μl of bisulfite-treated DNA, and a set of primers; UM1 (see above) and m-L2D: 5'-CAA AAR RCT TTA TTR RAT ACA C-3'.
Cloning and sequencing of PCR products
PCR products were purified using a QIAquick Gel Extraction Kit (Qiagen) followed by cloning into the pGEM®-T Easy vector (Promega, Madison, WI). Plasmid DNA was prepared using a QIAprep Spin Miniprep Kit (Qiagen) for sequencing at Functional Biosciences (Madison, WI).
ERV mining, sequence alignment, and phylogenetic analyses
The National Center for Biotechnology Information (NCBI) BLASTN and BLASTP programs were alternately used for mining new ERVs from the C57BL/6J mouse genome database with a 40 nucleotide probe, which was serendipitously identified in our previous study (unpublished). Alignment and phylogenetic analyses of the DNA, including the bisulfite-converted DNA clones, and protein sequences were performed using the MegAlign program from DNASTAR (Madison, WI).
The significance of differences in the C to T conversion rate at individual cytosine residue positions (plus and minus strands) was evaluated by Fisher's Exact probability test. The differences in the number of converted cytosine residues in the ERVmch8 sequence between the cerebellum and the other five brain compartments were evaluated by a Student's t-test. P values of less than 0.05 were determined to be significant.
Acknowledgements and funding
This study was supported by grants from Shriners of North America (No. 86800 to KC, No. 84302 to KHL [postdoctoral fellowship]) and the National Institutes of Health (R01 GM071360 to KC).
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