- Open Access
Selective translational repression of HIV-1 RNA by Sam68DeltaC occurs by altering PABP1 binding to unspliced viral RNA
Retrovirologyvolume 5, Article number: 97 (2008)
HIV-1 structural proteins are translated from incompletely spliced 9 kb and 4 kb mRNAs, which are transported to the cytoplasm by Crm1. It has been assumed that once in the cytoplasm, translation of incompletely spliced HIV-1 mRNAs occurs in the same manner as host mRNAs. Previous analyses have demonstrated that Sam68 and a mutant thereof, Sam68ΔC, have dramatic effects on HIV gene expression, strongly enhancing and inhibiting viral structural protein synthesis, respectively. While investigating the inhibition of incompletely spliced HIV-1 mRNAs by Sam68ΔC, we determined that the effect was independent of the perinuclear bundling of the viral RNA. Inhibition was dependent upon the nuclear export pathway used, as translation of viral RNA exported via the Tap/CTE export pathway was not blocked by Sam68ΔC. We demonstrate that inhibition of HIV expression by Sam68ΔC is correlated with a loss of PABP1 binding with no attendant change in polyadenosine tail length of the affected RNAs. The capacity of Sam68ΔC to selectively inhibit translation of HIV-1 RNAs exported by Crm1 suggests that it is able to recognize unique characteristics of these viral RNPs, a property that could lead to new therapeutic approaches to controlling HIV-1 replication.
Expression of the full coding potential of the HIV-1 genome is dependent upon a number of post-transcriptional processes. The primary 9 kb transcript from the integrated provirus can be spliced into over 30 mRNAs through suboptimal splicing events [1–4]. Resulting HIV-1 mRNAs can be grouped into three classes: the unspliced, 9 kb class, encoding Gag and GagPol; the singly spliced, 4 kb class, encoding Vif, Vpr, Vpu and Env; and the multiply spliced, 2 kb class, encoding Tat, Rev and Nef. Incompletely spliced mRNAs are normally retained in the nucleus but the virus has evolved a mechanism for the transport of the 9 kb and 4 kb viral mRNAs to the cytoplasm. The Rev protein is translated in the cytoplasm, then shuttles into the nucleus where it multimerizes on the Rev Response Element (RRE) contained in the introns of the incompletely spliced HIV-1 mRNAs. Once Rev binds to the RNA, its nuclear export signal (NES) interacts with Crm1 and mediates export to the cytoplasm [5, 6].
HIV-1 gene expression may be controlled at several steps including transcription, splicing, polyadenylation, nuclear export and translation [3, 4, 7]. All of these processes depend upon host cell factors . Recent work in our laboratory has focused on Sam68, a member of the STAR/GSG family of proteins . These proteins contain an RNA binding motif, the KH domain, embedded within a larger conserved GSG (Gld1, Sam68, GRP33) domain, which mediates multimerization. Sam68 is a nuclear, non-shuttling protein, and contains both proline- and tyrosine-rich domains mediating binding to multiple kinases (i.e. Src, Lck, Sik/BRK, ZAP-70) through SH3 and SH2 domains, respectively [9, 10]. Given its interaction with kinases involved in signal transduction, Sam68 has been suggested to serve as a signal mediator that affects multiple cellular processes including cell cycle regulation, tumour suppression, alternative splicing, and RNA 3' end formation [9–17]. More pertinent to HIV-1, overexpression of Sam68 and other members of the GSG family have been shown to significantly enhance HIV-1 gene expression [18–21]. Sam68 can also enhance expression of HIV-1 mRNAs exported to the cytoplasm via the constitutive transport element (CTE) of Mason-Pfizer monkey virus by promoting utilization by the translational apparatus of the cell . Two groups have reported that depletion of Sam68 results in the loss of HIV-1 structural protein expression in several cell lines [23–25].
In contrast to the full length protein, a truncation mutant of Sam68 lacking the C-terminal 112 amino acids, Sam68ΔC, is a potent inhibitor of HIV-1 protein expression [19, 21]. Unlike Sam68, Sam68ΔC is localized predominantly in the cytoplasm and its inhibitory function requires this distribution . Therefore, differences in activity between Sam68 and Sam68ΔC likely reflects the different protein-protein interactions available in the different compartments of the cell. Previous experiments in our lab showed that Sam68ΔC induced accumulation of HIV-1 4 kb mRNAs into perinuclear bundles suggesting that it might act by sequestering the viral RNA from the translational apparatus . In this study, we set out to define the mechanism and specificity of Sam68ΔC inhibition. We show that Sam68ΔC specifically inhibits RRE containing mRNAs. We also demonstrate that depolymerization of microfilaments disrupted the perinuclear bundles, dispersing the viral RNA throughout the cytoplasm, but failed to restore the synthesis of the HIV-1 structural proteins (Gag, Env). This finding suggests that the block to expression is at the level of engagement with the translational apparatus. Subsequent analysis of HIV-1 env mRNA distribution in polysome gradients in the presence and absence of Sam68ΔC supports this conclusion. Our studies determined that Sam68ΔC has no effect on viral RNA polyadenylation or poly(A) tail length. Inhibition of translation by Sam68ΔC was not associated with any changes in viral RNA localization, abundance, or processing but is correlated with changes in the composition of the mRNP. We show that Sam68ΔC inhibition of HIV-1 mRNA translation is accompanied by a reduction in PABP1 association with the affected mRNAs.
Susceptibility to Sam68ΔC repression is conferred by the nuclear export pathway
The ability of Sam68ΔC to selectively suppress expression of the 9 kb and 4 kb classes of HIV-1 mRNAs suggested that there is some unique feature that renders them susceptible to repression. Cellular mRNAs use the Tap export pathway, while HIV-1 9 and 4 kb RNAs are incompletely spliced and contain sequences preventing their export by Tap [26–33]. These incompletely spliced HIV-1 RNAs are exported from the nucleus via the interaction of HIV-1 Rev with the host protein Crm1 [5, 6, 34]. Two possible explanations for repression of the 9 kb and 4 kb HIV-1 RNAs by Sam68ΔC are immediately apparent: either they contain unique RNA sequences recognized by Sam68ΔC or export via the Crm1 pathway marks the viral RNA for inhibition. To address this question directly, we examined the ability of Sam68ΔC to inhibit expression of HIV-1 Gag RNAs utilizing different nuclear export elements; the constitutive transport element (CTE) from Mason-Pfizer Monkey virus that interacts directly with Tap (Gag-CTE), or the RRE that requires Rev and Crm1 (Gag-RRE) (Fig. 1a) . Gag RNA generates a 55 kDa polyprotein that is subsequently processed by the viral protease into matrix (p17), capsid (p24), nucleocapsid (p9) and p6. Gag expression was measured by anti-Gag western blot in which the p55 precursor and p24 are detected. GagRRE expression is dependent upon Rev and is reduced to baseline by Sam68ΔC (Fig. 1b,c). Parallel western blots demonstrated that Sam68ΔC does not markedly alter Rev levels (Fig. 1b). In contrast to the Gag-RRE reporter, Sam68ΔC had no significant effect on expression from Gag-CTE (Fig. 1b,c). This demonstrates that it is not the Gag sequence, but rather the RRE and/or the Crm1 export pathway that dictates inhibition by Sam68ΔC.
Perinuclear bundling of HIV-1 RNA by Sam68ΔC does not account for translation inhibition
Previously, we reported that Sam68ΔC expression induced accumulation of both unspliced viral RNA and Sam68ΔC in bundles at the outer periphery of the nucleus . As shown in Fig. 2a, in the absence of Sam68ΔC, HIV-1 env RNA is distributed throughout the cytoplasm. The formation of the perinuclear bundles upon co-expression of Sam68ΔC, as seen in Fig. 2b, suggested that Sam68ΔC might be sequestering the RNA from the translational apparatus. If true, disruption of these complexes should restore translation of the viral RNAs. We examined the effect of various agents which disrupt the cytoskeleton on the integrity of the Sam68ΔC/viral RNA perinuclear bundles, and the expression of viral proteins (Fig. 2c–f)). To minimize secondary drug effects, the minimum amount of each drug required to depolymerize its target in one hour was used (data not shown). While treatment of cells with either nocodazole or colcemid to disrupt microtubules had no effect on localization of viral RNA or Sam68ΔC (Fig. 2c,d), disassembly of the microfilaments by treatment with cytochalasin D or latrunculin B resulted in dispersal of both viral RNA and Sam68ΔC throughout the cytoplasm (Fig. 2e, f). The distribution of the viral RNA which has been released from the perinuclear bundles is similar to that seen in the absence of Sam68ΔC (Fig. 2a).
Next we questioned whether the released viral RNA was translated. Cells were transfected with Gag-RRE and Rev expression vectors in the presence or absence of Sam68ΔC. Forty-eight hours post-transfection, cells were treated with the drugs as before and synthesis of viral protein was monitored by incubation in the presence of 35S methionine. Cells were lysed, the 35S-labelled Gag immunoprecipitated, the immunoprecipitates run out on SDS-PAGE gels, and the gels exposed to a phosphorscreen overnight (Fig. 2g). The 55 kDa Gag polyprotein (p55) was not detected in the immunoprecipitates from the mock transfected cells but only in immunoprecipitates from cells transfected with Gag-RRE and Rev (Fig. 2g, lanes 1–5 versus 6). Therefore, the immunoprecipitation was specific and the p55 signal could be used as a measure of Gag-RRE translation. None of the drugs had any effect on the level of Gag expression in the absence of Sam68ΔC indicating that they had no significant effect on translation (Fig. 2g, lanes 1–6). None of the drugs induced expression of Gag in the presence of Sam68ΔC (Fig. 2g, lanes 7–12) despite latrunculin B/cytochalasin D shifting the subcellular distribution of both Sam68ΔC and the viral RNA. Therefore, integrity of the Sam68ΔC/viral RNA perinuclear bundles is not essential for translational repression. This observation suggests that Sam68ΔC inhibits viral RNA translation, not by changing its subcellular distribution, but blocking interaction with the translational apparatus. This effect could be achieved either through alterations in viral RNA structure or composition of the mRNP.
Sam68ΔC inhibits HIV envRNA recruitment into heavy polysomes
To confirm that Sam68ΔC was acting at the level of translation, cytoplasmic extracts were fractionated on linear sucrose gradients (Fig. 3). Fractions collected were subsequently analyzed for the presence of Sam68ΔC, ribosomal protein L26, actin mRNA and unspliced HIV env RNA. As shown in Fig. 3b, Sam68ΔC is predominately found at the top of the gradient but a significant amount is present in the heavier fractions consistent with previous observations of an association between Sam68 and ribosomes . Addition of EDTA to disrupt polysomes resulted in a shift in Sam68ΔC distribution to the top of the gradient, suggesting that there might be interaction of Sam68ΔC with polysomes. Parallel analysis of actin mRNA (Fig. 3c) revealed that the bulk of this RNA is found within heavy polysomes in the presence or absence of Sam68ΔC. EDTA addition induced a shift in distribution to lighter gradient fractions consistent with an association with polysomes. In contrast, unspliced HIV-1 env RNA underwent a shift in distribution from being predominately in the polysome fraction to predominately in the mRNP/monosome fraction in the presence of Sam68ΔC (Fig. 3d). The distribution of unspliced env RNA in the presence of Sam68ΔC significantly overlaps with that seen upon addition of EDTA consistent with a selective inhibition of translation of this mRNA.
Mapping of domains within Sam68ΔC essential for repression of HIV-1 gene expression
Sam68 contains a number of well-defined domains that mediate specific RNA binding (KH-domain), non-specific RNA binding (RGG-boxes) or protein-protein interactions (proline-rich domains and tyrosine-phosphorylation sites) . We made a number of deletions of Sam68ΔC to define a minimal inhibitory mutant (Fig. 4a,b). As shown by western blots (Fig. 4b), all mutants were equally expressed and subsequent immunofluorescence microscopy confirmed that all were localized to the cytoplasm (data not shown). Previous analyses have determined that the region spanning amino acids (a.a) 269–321 is essential for the inhibitory property of Sam68ΔC . In our work, Sam68ΔCmin, spanning amino acids 14 to 300 with an internal deletion of amino acids 45 to 54 (encompassing an RGG box), was the minimal construct that retained significant inhibitory activity (Fig. 4b). In contrast, deletion of the first 28 (Sam68Δ28ΔC) or last 70 (Sam68:5–262) amino acids of Sam68ΔC resulted in a loss of inhibitory activity (Fig. 4b). These observations indicate that domains at the N- and C-terminus of Sam68ΔC are essential to its inhibitory activity.
Splicing, cleavage, and polyadenylation are tightly coupled events within the nucleus. However, in the case of HIV-1 RNA, numerous forms of viral RNA are generated, some of which have failed to undergo one or more of these steps. This is essential for the production of the HIV-1 structural proteins and therefore, the HIV-1 lifecycle. The HIV-1 env reporter, pgTat, expresses gp160/120 from the unspliced, cleaved mRNA (see Fig. 4a) . Previously, we showed that full-length Sam68 increases the amount of unspliced, cleaved pgTat mRNA available for polyadenylation, export and translation into gp160/120 . We have also shown that restoring nuclear accumulation of Sam68ΔC by addition of a heterologous nuclear localization signal (NLS) converted the protein into a stimulator of Rev function comparable to full length Sam68 . In light of these results, we wanted to assess whether Sam68ΔC inhibition is due to alterations in the abundance or processing of env RNA.
We examined the extent of splicing and cleavage of pgTat RNA by RNase protection assay (RPA). As illustrated in Fig. 4a, the RPA probe used in this analysis spans both the 3' splice site and the polyadenylation site, yielding four RNase protection products: unspliced, uncleaved (US-UC); unspliced, cleaved (US-C); spliced, uncleaved (S-UC); and spliced, cleaved (S-C) (Fig. 4a). Sam68ΔC did not cause any marked change in the amount of US-C RNA that could account for the loss of gp160/120 expression but a reduction in levels of S-C RNA was consistently observed (Fig. 4c) that might reflect effects on either viral RNA splicing or S-C RNA stability. In contrast, the mutant, Sam68:5–262, increased the abundance of US-C RNA (Fig. 4c). The stimulation of cleavage of unspliced RNA by Sam68:5–262 is consistent with the known activity of full length Sam68 in promoting RNA polyadenylation previously reported by our laboratory  and define that different domains of Sam68 are required for inhibitory versus stimulatory activity.
Inhibition by Sam68ΔC is not associated with changes in polyadenylation of envRNAs
De-adenylation of mRNA is a common form of translational repression . To assess changes in the polyadenylation state of env mRNAs in the presence of Sam68ΔC, total RNA was extracted and the polyadenylated RNA isolated using oligo(dT)25 beads. Given that cleavage and polyadenylation are tightly coupled processes, all cleaved RNAs are expected to have a poly(A) tail and bind to the oligo(dT)25 column. Appearance of cleaved RNA in the poly(A)- fraction would be indicative of a de-adenylation event. Analysis of the poly(A)- and poly(A)+ fractions by RPA revealed that the fractionation was successful; uncleaved env RNAs (US-UC and S-UC) being predominantly in the poly(A)- fraction and cleaved versions (US-C and S-C) in the poly(A)+ fraction (Fig. 5a). Sam68ΔC did not shift the distribution of the unspliced RNAs between the fractions, indicating that the US-C form of pgTat RNA retained a poly(A) tail of sufficient size to bind to the column (Fig. 5a).
To assay whether there were any significant changes in poly(A) tail length, we used a random amplification of cDNA ends, polyadenylation test (RACE-PAT) . A primer consisting of a specific 15-mer sequence followed by 20 T residues was used to make cDNA. This primer anneals randomly along the length of the poly(A) tail, generating cDNAs with a range of lengths corresponding to where on the poly(A) tail cDNA synthesis was initiated. Using a reverse primer complementary to the 15-mer sequence and a env specific forward primer specific to either spliced (S F) or unspliced (US F) env RNA, we measured the length of the polyA tail of each RNA species (Fig. 5b). Without a poly(A) tail, the spliced amplicon is 340 nucleotides and the unspliced amplicon is 415 nucleotides. Therefore, the tail length of spliced pgTat RNA is approximately 10–60 nucleotides and that of the unspliced RNA is 200–250 nucleotides (Fig 5b). No change in amplicon size was observed upon addition of Sam68ΔC. Thus, the loss of gp120 expression upon Sam68ΔC co-transfection cannot be attributed to de-adenylation of unspliced env mRNA.
Sam68ΔC alters the association of Rev-dependent viral RNAs with PABP1
PABP1 has been shown to be an important promoter of mRNA translation through its direct interaction with eIF4G and resultant indirect interactions with the eIF4E cap binding protein [40, 41]. Therefore, we examined PABP1 association with pgTat mRNA by RNP immunoprecipitation (RIP). PABP1 levels were not affected by expression of the Sam68 proteins and there was no change in epitope availability since precipitation of PABP1 was similar for each sample (Fig 6a). RNA analysis determined that pgTat RNAs were specifically precipitated with PABP1 as compared to control rabbit IgG RIPs (Fig. 6b). The S-C form of pgTat was the major isoform pulled down with anti-PABP-1 antibody but US-C RNA was also detected in the immunoprecipitates. Unexpectedly. some immunoprecipitation of both US-UC and S-UC was also detected particularly in the presence of the Sam68Δ28ΔC and Sam68:5–262. The reason for this is unclear but might reflect an indirect interaction of PABP1 with these RNAs (that lack a polyA tail). PABP1 association was quantitated by comparing the ratio of US-C to S-C pgTat RNA in the RIP compared to the input (Fig. 6c). Sam68Δ28ΔC and Sam68:5–262 had no significant effect on US-C RNA association with PABP1. Sam68ΔC and Sam68ΔCmin significantly reduced US-C RNA association with PABP1 (p < 0.05, Fig. 6b,c). Expression of Sam68ΔC also had no effect on the immunoprecipitation of RNPs containing the S/C form of pgTat RNA, consistent with Sam68ΔC being able to discriminate between the US-C and S-C forms of the viral RNA.
To confirm that Sam68ΔC induced similar changes in PABP1 association in the context of the HIV-1 provirus, the experiment was repeated using a replication inactive form of the virus (HxBruR-/RI-). Sam68ΔC strongly reduced both p24 and gp160/120 expression from this construct (Fig. 7). However, the mutants have slightly different effects on the provirus than on the env reporter construct pgTat. While Sam68:5–262 enhances expression of gp160/120 and p24, Sam68Δ28ΔC and Sam68ΔCmin have little effect. None of the mutants had any effect on Rev or PABP1 protein levels (top band, Fig 7, Fig. 8a). PABP1 was immunoprecipitated uniformly in all samples (Fig. 8a). The proviral RNA associated with PABP1 was analyzed using an RPA probe that spans the 5' splice site within Gag, yielding 2 protection products. One protection product corresponds to the 9 kb and the other to the spliced forms (2 and 4 kb classes) of HIV-1 mRNAs. As translation of both the 9 kb (p24) and 4 kb (gp160/120) classes of viral RNAs are affected by Sam68ΔC, the band corresponding to the 2 and 4 kb classes could not be used for quantitation. Relative PABP1 association was quantitated by the ratio of 9 kb RNA to actin RNA in the RIP compared to the input. Sam68ΔC significantly decreased PABP1 association with HIV-1 9 kb RNA relative to pcDNA, while Sam68:5–262 significantly increased PABP1 association with HIV-1 9 kb RNA (p < 0.01, Fig. 8b,c). Both Sam68Δ28ΔC and Sam68ΔCmin showed slight reductions in PABP1 association with 9 kb viral RNA but these were not found to be significant even at a higher p-value (p < 0.05, Fig. 8b,c). Therefore, similar to the results seen with the pgTat vector, changes in viral protein expression are correlated with extent of interaction of the corresponding RNA with PABP1.
Recent studies have highlighted the changes in RNP composition that occur during mRNA processing, export and surveillance as the RNA moves from its site of synthesis through the nuclear pore to the translational apparatus. In particular, a number of hnRNP proteins are removed, the exon junction complex (EJC) is added following splicing then subsequently removed during translation, and there is an exchange of factors at the extreme 5' and 3' ends of the RNA (CBP20/80 and PABP2 are exchanged for eIF4E and PABP1, respectively) [42–44]. What remains unknown is the extent to which the protein composition of a specific RNP is determined by its sequence or its processing pathway. In the case of the EJC, the act of splicing deposits the complex ~20–25 nt 5' of the splice site in a sequence independent fashion . In other instances, binding of factors to specific sequence elements (Zipcode localization elements, AU-rich elements) affects localization, stability or translation of an RNA . It has generally been assumed that, despite an alternative export pathway, translation of incompletely spliced HIV-1 mRNAs occurs in the same manner as host mRNAs. However, in this study we provide evidence that Sam68ΔC is able to discriminate between RNAs based, in part, on the export pathway used (Fig. 1). What is unclear is whether export by Crm1 alone is sufficient to confer inhibition by Sam68ΔC or if additional features of the HIV-1 incompletely spliced RNAs (perhaps the Rev/RRE complex) provide additional recognition elements that confer regulation by this factor. Some data (not shown) supports the latter hypothesis.
Translational control has been studied extensively, and there are several well-defined mechanisms: changes in subcellular distribution (stress granules, P-bodies) [45, 46], changes in RNA structure (de-adenylation, cytoplasmic adenylation), destabilizing RNA elements (AREs) [47, 48], and blocking eIF4E initiation (4E-BPs)  to name a few. Our data show that inhibition by Sam68ΔC is not dependent upon changes in mRNA subcellular distribution (Fig. 2). Polysome gradient analysis (Fig. 3) demonstrated that Sam68ΔC selectively blocks the association of unspliced HIV-1 env RNA with the polysome fraction, consistent with a block in one of the steps of translation. However, data acquired to date does not permit us to evaluate whether the block is at initiation or subsequent elongation. Future experiments will attempt to address this issue. These observations conflict with a previous report by Zhang et al that suggested the mechanism of inhibition was a block to nuclear export . As evidence of this transport block, they performed nuclear/cytoplasmic fractionation in which Sam68ΔC sensitive mRNAs co-sedimented with the nuclear fraction. We clearly see that the inhibited mRNAs are in perinuclear bundles in the cytoplasm, in the presence of Sam68ΔC (Fig. 2b). However, their association with the microfilaments makes these mRNPs insoluble, and as a result they co-sediment with the nuclei and would be incorrectly scored as untransported (Fig. 2b, data not shown). Sam68ΔC does not affect target mRNA abundance (Fig. 4c), polyadenylation (Fig. 5a), or poly(A) tail length (Fig. 5b), only PABP1 binding (Fig. 6, 8). The reduction in PABP1 binding to Rev-responsive viral RNAs by Sam68ΔC could be achieved either by blocking PABP2 exchange for PABP1 following export, or removing PABP1 from the mRNA once it has engaged the translational apparatus. We believe that inhibition of PABP1 binding to an mRNA, by Sam68ΔC, represents a novel mode of translation inhibition but further studies will be necessary to identify the stage of cytoplasmic RNA processing being affected by Sam68ΔC.
Previous analyses had demonstrated that inhibition of HIV expression by Sam68ΔC was lost upon introduction of mutations that disrupted RNA binding capacity consistent with a direct interaction with the affected RNA being required . Mutational analysis shown in this study indicates that additional domains of Sam68ΔC are essential to its inhibitory potential; amino acids 14 to 44 and amino acids 262 to 300. Since neither of these regions has been implicated in RNA binding, the findings suggest that these regions might be involved in mediating protein-protein interactions. Interestingly, the requirements for inhibition appear distinct from those promoting unspliced RNA polyadenylation and enhancement of HIV gene expression as further C-terminal mutations (Sam 5–262) converted the protein from an inhibitor to a stimulator. While the regions encompassed by Sam68ΔCmin were sufficient to inhibit HIV expression in several different assays, other data suggests that it might have reduced function. Sam68ΔC consistently inhibited expression of the incompletely spliced HIV-1 mRNAs and had a corresponding effect on PABP1 binding. However, the mutants had different effects on pgTat and HxBruR-/RI- reporters. Importantly, the effect of the mutants on viral protein synthesis was mirrored in the effects they had on PABP1 binding. For instance, Sam68:5–262 had a limited effect on gp160/120 expression from pgTat, but increased the amount of gp160/120 and p24 from HxBruR-/RI-. Correspondingly, Sam68:5–262 had no significant effect on PABP1 binding to US-C pgTat mRNA but did significantly increase PABP1 binding to the 9 kb HxBruR-/RI- mRNA. In contrast, Sam68ΔCmin was able to block expression of pgTat RNA but not the 9 kb and 4 kb RNAs of the provirus. Analysis of the effect of Sam68ΔCmin revealed that it decreased binding of PABP1 to US/C pgTat RNA but resulted in no or little alteration in PABP1 interaction with viral RNAs. Given that regions affecting inhibitory function are outside of the domain required for RNA binding (GSG and RGG motifs), the differences among the Sam68ΔC mutants likely reflect changes in the interaction with host factors. These factors could facilitate or inhibit remodeling of the viral RNP following export from the nucleus and their activity could vary depending upon subtle differences in the processing/composition of the RNPs being affected.
The perinuclear accumulation of viral RNA induced by Sam68ΔC, while not essential for the translational inhibition, is similar in phenotype to the effect seen upon depletion of human Rev-interacting protein (hRIP) . This finding suggests that both Sam68ΔC and hRIP may be influencing a similar step in viral RNA metabolism. These observations are consistent with a model whereby the viral RNP and hRIP interact at the nuclear periphery (directly or indirectly) to initiate remodeling of the viral RNP to enhance its translation, a part of which is the exchange of PABP2 for PABP1. Sam68ΔC may interfere with this remodeling and prevent binding of PABP1, thereby inhibiting translation initiation (Figure 9). The recent identification of two RNA helicases (DDX1 and DDX3) that play essential roles in Rev function suggests that remodeling of the viral RNP plays an important role in ensuring efficient expression of the HIV-1 structural genes [51, 52]. How and where these helicases act remains to be determined.
In summary, we have discovered that Sam68ΔC is able to use the features of Crm1 mRNA export to specifically inhibit translation of Rev-dependent HIV-1 mRNAs. Inhibition occurs by a novel mechanism: inhibiting the association of PABP1 with the target mRNA. Based on the data presented here, and other published work, we hypothesize that viral mRNAs transported via the Crm1 export pathway have a unique mechanism through which they engage the translation machinery, possibly involving hRIP, DDX1 and DDX3 (Fig. 9). If true, it would indicate that mRNAs using different export pathways have distinct RNP compositions and therefore, separate control mechanisms. Future research into the details of translation initiation for Rev-dependent HIV-1 mRNAs may lead us to discover further discrepancies from host mRNAs and lead to new therapeutic approaches to control HIV-1 gene expression.
Materials and methods
The following constructs have been previously described: SV-Hygro, SV-H6Rev, CMVmyc3xterm , pDM128 , pgTat , Bl-env-HindIII , Sam68, and Sam68ΔC . Gag-CTE and Gag-RRE plasmids were provided by Dr. K. Boris-Lawrie, Ohio State University. HxBruR-/RI- was provided by Eric Cohen, Universite de Montreal. Sam68Δ28ΔC was generated by PCR using primers: 5'-CGGAATTCCCCTCGGTGCGTCTGA-3' and 5'-CGGGATCCAGTCACAGTGG-CACCTCT-3'. The amplicon was digested with EcoRI and BamHI and ligated into CMVmyc3xterm. Sam68:5–262 was made by EcoRI and EcoRV digest of Sam68ΔC and ligated into the EcoRI and SmaI sites of CMVmyc3xterm. Sam68ΔCmin was made in a stepwise fashion. First, Sam68:5–300 was generated by PCR using primers 5'-CCATTAACGCAAATGGGCGGTA-3' and 5'-CCGCTCGAGAACAGG-TGGAGG-3'. The amplicon was digested with EcoRI and XhoI and ligated into Sam68ΔC. To generate internal deletions, Quickchange mutagenesis (Stratagene) was carried out using Sam68:5–300 as a template: Δ14: 5'-GGGGAATTCGAGAAGATCGGGCCGCAGCTGGC-3' and 5'-GCAGCTGCGG-CCCGATCTTCTCGAATTCCC-3'; Δ(45–54): 5'-GCTTCCTCAC-CGGCCCGCTCGGGCCTCGCCC-3' and 5'-GGGCGAGGCCCGAGCGGGCCGG-TGAGGAAGC-3'. Bl-actin was generated by PCR from cDNA using primers: 5'-GCTACGAGCTGCCTGACG-3' and 5'-TCCTTCTGCATCCTGTCG-3'. The amplicon was cloned into the EcoRV site of Bluescript. Bl-SD-Gag was amplified from HxBruR-/RI- using: 5'-CGCGGATCCGAAGTAGTGTGTGCCCGTCT-3' and 5'-CCCAAGCTTCCCTGCTTGCCCATACTATA-3'. The amplicon was digested with BamHI and HindIII and ligated into Bluescript.
Cell lines and transfections
HeLa and 293T cells were maintained in Iscove's modified Dulbecco's medium (IMDM) supplemented with 10% fetal bovine serum (FBS), 50 μg/mL gentamycin sulfate and 2.5 μg/mL amphotericin B. Vectors were introduced to 293T cells by calcium phosphate transfection . Vectors were introduced to HeLa cells by Fugene 6 transfection reagent (Roche) following manufacturer's protocol. Cells were harvested two days post-transfection.
The following antibodies were used: mouse anti-myc (Invitrogen), mouse anti-tubulin (Sigma), mouse anti-gp120AK (courtesy of H. Schaal), mouse anti-p24 (clone 183-1112-5C), rabbit anti-L26 (Cell Signaling Technology) and rabbit anti-human PABP1 (aa 462–633) (courtesy of N. Sonenburg, McGill University). The following secondary antibodies were used: donkey anti-rabbit IgG conjugated HRP, donkey anti-mouse IgG conjugated HRP, donkey anti-rabbit IgG conjugated Texas red, donkey anti-mouse IgG conjugated Texas red (Jackson Immuno Research), protein G conjugated HRP (Molecular Probes), and sheep anti-digoxigenin conjugated FITC (Boehringer Mannheim).
6 × 105 293T cells were transfected as follows: 0.5 μg GagRRE, 0.1 μg SV-Hygro or SV-H6Rev, and 2.0 μg pcDNA3.1 or Sam68ΔC. For Rev-independent expression cells were transfected with 2.0 μg GagCTE, and 2.0 μg pcDNA3.1 or Sam68ΔC. Cells were harvested in whole cell lysis buffer (150 mM NaCl, 10 mM Na2HPO4, 1% Triton X-100, 0.1% SDS, 0.2% sodium azide, 0.5% sodium deoxycholate, 1 mM sodium orthovanadate), fractionated on SDS-PAGE gels and transferred to PVDF membrane (Pall Life Sciences). Bound antibodies were detected using Western Lightning (Perkin Elmer). For quantitation of western blots, films were scanned and analyzed using Imagequant software. Signals for both p55 and p24 were combined for analysis and data normalized using the tubulin signals. Quantitation shown was generated from three independent experiments.
In Situhybridization and immunofluorescence
3 × 105 HeLa cells were transfected with 0.25 μg SV H6Rev, 1.25 μg pgTat and 5.0 μg pcDNA3.1 or Sam68ΔC. 48 hours post-transfection, cells were treated with fresh media containing no drug, colcemid (0.1 μg/mL), nocodazole (1.0 μg/mL), cytochalasin D (0.5 μg/mL), or latrunculin B (0.5 μg/mL) for 2 hours prior to fixation. In situ hybridization was performed as previously described . Digoxigenin-labeled Env-HindIII probe, antisense to HIV-1 env mRNA, was made with 10 × digoxigenin RNA labeling kit (Roche). Myc-tagged proteins were detected with monoclonal anti-myc antibody (Invitrogen) as previously described . Immunofluorescence was detected using a Leica DMR microscope at either 400× or 630× magnification.
6 × 105 HeLa cells were transfected with 0.2 μg SV-H6Rev, 1.0 μg Gag-RRE and 4.0 μg pcDNA3.1 or Sam68ΔC. 48 hours post-transfection, cells were treated with drugs for 2 hours, as outlined above. Cells were then labeled with 100 μCi of 35S- methionine for 4 hours. Cells were harvested in whole cell lysis buffer then diluted with 3 volumes IPP150 (150 mM KCl, 10 mM Tris-HCl pH 7.5, 0.1% NP40, 0.1% sodium azide). The precleared lysates were incubated with anti-p24 antibody for 1 hour at 4°C, then immunoprecipitated with Gammabind plus sepharose (GE Healthcare) for 1 hour. Beads were washed with IPP150 buffer and the immunoprecipitated protein was run out on 10% SDS-PAGE. Labeled proteins were detected following exposure to a Phosphor Imager screen.
3 × 106 293T cells were transfected with 0.4 μg SV-Hygro or SV-H6Rev, 2.0 μg pgTat and 8.0 μg of pcDNA3.1, Sam68ΔC or mutants thereof. 48 hours post-transfection 25% of the cells were harvested in whole cell lysis buffer for protein analysis, and 75% of the cells were harvested for RNA isolation . RNase protection assays (RPA) were performed as previously described . To monitor the polyadenylation status of the pgTat mRNA 10 μg total RNA was selected using oligo(dT)25 beads according to manufacturers directions (Dynal Biotech). The selected RNA was then input into the RNase protection assay using Bl-Tat X/X probe . RACE-PAT (random amplification of cDNA ends-polyadenylation test) cDNA was synthesized using an anchor primer (5'-CTCGCCGGACACGCTGAACTTTTTTTTTTTTTTTTTTTT-3') with MMLV-RT (Invitrogen). The cDNA generated was then used to generate both spliced and unspliced amplicons using the forward spliced (5'-AGCGGAGACAGCGACGAAGAG-3') or unspliced (5'-CGACCTGGATGGAGTGGGACA-3') and the reverse primer (5'-CTCGCCGGACACGCTGAAC-3'). Amplification used the following cycle parameters: 94°C, 1 minute; 66°C, 1 minute; 72°C, 2 minute; for 30 cycles. Amplicons were fractionated on native PAGE gels and detected by exposure to Phosphor Imager screen. For proviral RNA analysis, 3 × 106 293T cells were transfected with 2.0 μg HxBruR-/RI- and 8.0 μg of Sam68ΔC or mutants thereof. The probes used for RNase protection assay were Bl-actin and Bl-SD-Gag.
Polysome analysis was performed as described by Li et al.  with the only modification being the addition of cytochalasin D (0.5 μg/ml) to the media 1 h prior to harvest. 0.5 ml fractions were collected following centrifugation and analyzed for protein or RNA distribution. For protein analysis, 5 μg/ml BSA was added to 200 μl of each fraction and proteins precipitated by addition of an equal volume of 40% TCA. After incubation at 4 C for 1 h, precipitate was collected by centrifugation, washed 5 times with acetone and resuspended in whole cell lysis buffer. Proteins were fractionated on SDS-PAGE gels and blotted onto PVDF membranes for detection of indicated proteins. In the case of RNA, following proteinase K/phenol-chloroform purification, RNA was ethanol precipitated, resuspended and treated with Turbo DNase 1 as per manufacturer's instructions (Ambion). RNA was then used for quantition of actin and env RNA by real time QRT-PCR using the following primer sets: actin forward 5'-GAG CGG TTC CGC TGC CCT GAG GCA CTC-3', actin reverse 5'-GGG CAG TGA TCT CCT TCT GCA TCC TG-3', env forward 5'-CAA CAA TGG GTC CGA GAT CTT-3', env reverse 5'-AGC TCC TAT TCC CAC TGC TC-3'. QPCR reactions were run in duplicate for each probe and gradient fraction.
3 × 106 293T cells were transfected with 0.4 μg SVH6Rev, 2.0 μg pgTat and 8.0 μg of pcDNA3.1, Sam68ΔC or mutants thereof. Whole cell lysates were generated by a modification of the protocol of Siomi et al . 48 hours post-transfection, cells were washed twice with 1 × PBS and once with Buffer A (110 mM potassium acetate, 2 mM magnesium acetate, 2 mM DTT, 10 mM HEPES pH 7.5). The cell pellet was resuspended in 400 μl Buffer B (10 mM potassium acetate, 2 mM magnesium acetate, 2 mM DTT, 5 mM HEPES pH 7.5, 20 μM cytochalasin D) and incubated on ice for 10 min. Cells were disrupted by passage through 25 gauge needle, 5 times. KCl was added to 100 mM final concentration. Cells debris was pelleted by centrifugation at 1,500 rpm. Supernatant was adjusted to 0.5% NP40 and incubated on ice for 20 min. Insoluble material was pelleted by centrifugation at 14,000 rpm and supernatant retained for use in subsequent immunoprecipitations. 1/42 of the supernatant was retained for analysis of the RNA input and 1/42 for analysis of protein input. For each immunoprecipitation, 10/42 of the soluble fraction was diluted with 10 volumes RIPA buffer (150 mM NaCl, 50 mM Tris-HCl pH 7.5, 1% NP40, 1 mM EDTA ph 7.5, 0.5% sodium deoxycholate, 0.05% SDS) and 100 u RNaseOUT (Invitrogen). The sample was precleared by incubation with Gammabind plus sepharose (GE Healthcare) for 1 h at 4°C. Supernatant was incubated overnight at 4°C with the indicated antibody. Immune complexes were collected by addition of Gammabind plus sepharose and incubation at 4°C for 1 h. The resin was washed 5× with 1 ml of RIPA buffer and then resuspended in 100 μl of RIPA. RNA was extracted from 150 μl of the resin slurry  and 50 μl of slurry was used for protein analysis following addition of 50 μl 2× dissociation buffer. Isolated RNA was subsequently used in RPA assays as outlined previously.
The data are presented as mean +/- one standard deviation. Data were compared using Student's t-test. * indicates a p-value < 0.05. ** indicates a p-value < 0.01.
Schwartz S, Felber BK, Benko DM, Fenyo E-M, Pavlakis GN: Cloning and functional analysis of multiply spliced mRNA species of human immunodeficiency virus type 1. J Virol. 1990, 64: 2519-2529.
Purcell D, Martin MA: Alternative splicing of human immunodeficiency virus type 1 mRNA modulates viral protein expression, replication, and infectivity. J Virol. 1993, 67: 6365-6378.
Cochrane AW, McNally MT, Mouland AJ: The retrovirus RNA trafficking granule: from birth to maturity. Retrovirology. 2006, 3: 18-10.1186/1742-4690-3-18.
Stoltzfus CM, Madsen JM: Role of viral splicing elements and cellular RNA binding proteins in regulation of HIV-1 alternative RNA splicing. Curr HIV Res. 2006, 4 (1): 43-55. 10.2174/157016206775197655.
Hope TJ: The ins and outs of HIV Rev. Arch Biochem Biophys. 1999, 365: 186-191. 10.1006/abbi.1999.1207.
Pollard V, Malim M: The HIV-1 Rev Protein. Ann Rev Micro. 1998, 52: 491-532. 10.1146/annurev.micro.52.1.491.
Tang H, Kuhen KL, Wong-Staal F: Lentivirus replication and regulation. Ann Rev Genet. 1999, 33: 133-170. 10.1146/annurev.genet.33.1.133.
Greene WC, Peterlin BM: Charting HIV's remarkable voyage through the cell: Basic science as a passport to future therapy. Nat Med. 2002, 8 (7): 673-680. 10.1038/nm0702-673.
Lukong KE, Richard S: Sam68, the KH domain-containing superSTAR. Biochim Biophys Acta. 2003, 1653 (2): 73-86.
Najib S, Martin-Romero C, Gonzalez-Yanes C, Sanchez-Margalet V: Role of Sam68 as an adaptor protein in signal transduction. Cell Mol Life Sci. 2005, 62 (1): 36-43. 10.1007/s00018-004-4309-3.
McLaren M, Asai K, Cochrane A: A novel function for Sam68: Enhancement of HIV-1 RNA 3' end processing. RNA. 2004, 10: 1119-1129. 10.1261/rna.5263904.
Richard S, Vogel G, Huot ME, Guo T, Muller WJ, Lukong KE: Sam68 haploinsufficiency delays onset of mammary tumorigenesis and metastasis. Oncogene. 2007
Paronetto MP, Achsel T, Massiello A, Chalfant CE, Sette C: The RNA-binding protein Sam68 modulates the alternative splicing of Bcl-x. J Cell Biol. 2007, 176 (7): 929-939. 10.1083/jcb.200701005.
Busa R, Paronetto MP, Farini D, Pierantozzi E, Botti F, Angelini DF, Attisani F, Vespasiani G, Sette C: The RNA-binding protein Sam68 contributes to proliferation and survival of human prostate cancer cells. Oncogene. 2007, 26 (30): 4372-4382. 10.1038/sj.onc.1210224.
Li QH, Fan TX, Pang TX, Yuan WS, Han ZC: Effect of proline rich domain of an RNA-binding protein Sam68 in cell growth process, death and B cell signal transduction. Chin Med J. 2006, 119 (18): 1536-1542.
Richard S, Torabi N, Franco GV, Tremblay GA, Chen T, Vogel G, Morel M, Cléroux P, Forget-Richard A, Komarova S, Tremblay ML, Li W, Li A, Gao YJ, Henderson JE: Ablation of the Sam68 RNA binding protein protects mice from age-related bone loss. PLoS Genet. 2005, 1 (6): e74-10.1371/journal.pgen.0010074.
Cheng C, Sharp PA: Regulation of CD44 alternative splicing by SRm160 and its potential role in tumor cell invasion. Mol Cell Biol. 2006, 26 (1): 362-370. 10.1128/MCB.26.1.362-370.2006.
Reddy TR, Xu W, Wong-Staal F: General effect of Sam68 on Rev/Rex regulated expression of complex retroviruses. Oncogene. 2000, 19: 4071-4074. 10.1038/sj.onc.1203749.
Reddy T, Xu W, Mau J, Goodwin C, Suhasini M, Tang H, Frimpong K, Rose D, Wong-Staal F: Inhibition of HIV replication by dominant negative mutants of Sam68, a functional homolog of HIV-1 Rev. Nature Med. 1999, 5: 635-642. 10.1038/9479.
Reddy TR, Suhasini M, Xu W, Yeh LY, Yang JP, Wu J, Artzt K, Wong-Staal F: A role for KH domain proteins (Sam68-like mammalian proteins and quaking proteins) in the post-transcriptional regulation of HIV replication. J Biol Chem. 2002, 277 (8): 5778-5784. 10.1074/jbc.M106836200.
Soros V, Valderrarama Carvajal H, Richard S, Cochrane A: Inhibition of Human Immunodeficiency Virus Type 1 Rev Function by a Dominant-Negative Mutant of Sam68 through Sequestration of Unspliced RNA at Perinuclear Bundles. J Virol. 2001, 75: 8203-8215. 10.1128/JVI.75.17.8203-8215.2001.
Coyle JH, Guzik BW, Bor YC, Jin L, Eisner-Smerage L, Taylor SJ, Rekosh D, Hammarskjold ML: Sam68 enhances the cytoplasmic utilization of intron-containing RNA and is functionally regulated by the nuclear kinase Sik/BRK. Mol Cell Biol. 2003, 23 (1): 92-103. 10.1128/MCB.23.1.92-103.2003.
Chen T, Richard S: A role for the GSG domain in localizing Sam68 to novel nuclear structures in cancer cell lines. Mol Biol Cell. 1999, 10: 3015-3033.
Li J, Liu Y, Kim BO, He JJ: Direct participation of Sam68, the 68-kilodalton Src-associated protein in mitosis, in the CRM1-mediated Rev nuclear export pathway. J Virol. 2002, 76 (16): 8374-8382. 10.1128/JVI.76.16.8374-8382.2002.
Modem S, Badri KR, Holland TC, Reddy TR: Sam68 is absolutely required for Rev function and HIV-1 production. Nucl Acids Res. 2005, 33 (3): 873-879. 10.1093/nar/gki231.
Brighty DW, Rosenberg M: A cis-acting repressive sequence that overlaps the Rev-responsive element of human immunodeficiency virus type 1 regulates nuclear retention of env mRNAs independently of known splicing signals. Proc Nat Acad Sci USA. 1994, 91: 8314-8318. 10.1073/pnas.91.18.8314.
Cochrane AW, Jones KS, Beidas S, Dillon PJ, Skalka AM, Rosen CA: Identification and characterization of intragenic sequences which repress human immunodeficiency virus structural gene expression. J Virol. 1991, 65: 5305-5313.
Graf M, Bojak A, Deml L, Bieler H, Wolf H, Wagner R: Concerted Action of Multiple cis-acting Sequences Is Required for Rev Dependence of Late Human Immunodeficiency Virus Type 1 Gene Expression. J Virol. 2000, 74: 10822-10826. 10.1128/JVI.74.22.10822-10826.2000.
Maldarelli F, Martin MA, Strebel K: Identification of post-transcriptionally active inhibitory sequences in human immunodeficiency virus type I RNA: novel level of gene regulation. J Virol. 1991, 65: 5732-5743.
Nasioulas G, Zolotukhin A, Tabernero C, Solomin L, Cunningham C, Pavlakis G, Felber B: Elements Distinct from Human Immunodeficiency Virus Type 1 Splice Sites Are Responsible for the Rev Dependence of env mRNA. J Virol. 1994, 68: 2986-2993.
Schwartz S, Felber BK, Pavlakis GN: Distinct RNA sequences in the gag region of human immunodeficiency virus type 1 decreases RNA stability and inhibit expression in the absence of Rev protein. J Virol. 1992, 66: 150-159.
Suh D, Seguin B, Atkinson S, Ozdamar B, Staffa A, Emili A, Mouland A, Cochrane A: Mapping of determinants required for the function of the HIV-1 env nuclear retention sequence. Virology. 2003, 310 (1): 85-99. 10.1016/S0042-6822(03)00073-4.
Schwartz S, Campbell M, Nasioulas G, Harrison J, Felber B, Pavlakis G: Mutational inactivation of an inhibitory sequence in human immunodeficiency virus type 1 results in Rev-independent gag expression. J Virol. 1992, 66: 7176-7182.
Rodriguez MS, Dargemont C, Stutz F: Nuclear export of RNA. Biol Cell. 2004, 96 (8): 639-655. 10.1016/j.biolcel.2004.04.014.
Cullen BR: Nuclear RNA export. J Cell Sci. 2003, 116 (Pt 4): 587-597. 10.1242/jcs.00268.
Paronetto MP, Zalfa F, Botti F, Geremia R, Bagni C, Sette C: The nuclear RNA-binding protein Sam68 translocates to the cytoplasm and associates with the polysomes in mouse spermatocytes. Mol Biol Cell. 2006, 17 (1): 14-24. 10.1091/mbc.E05-06-0548.
Zhang J, Liu Y, Henao J, Rugeles MT, Li J, Chen T, He JJ: Requirement of an additional Sam68 domain for inhibition of human immunodeficiency virus type 1 replication by Sam68 dominant negative mutants lacking the nuclear localization signal. Gene. 2005, 363: 67-76. 10.1016/j.gene.2005.06.043.
Colgan DF, Manley JL: Mechanism and regulation of mRNA polyadenylation. Genes Dev. 1997, 11 (21): 2755-2766. 10.1101/gad.11.21.2755.
Salles FJ, Richards WG, Strickland S: Assaying the polyadenylation state of mRNAs. Methods. 1999, 17 (1): 38-45. 10.1006/meth.1998.0705.
Mangus DA, Evans MC, Jacobson A: Poly(A)-binding proteins: multifunctional scaffolds for the post-transcriptional control of gene expression. Genome Biology. 2003, 4 (7): 223-10.1186/gb-2003-4-7-223.
Kahvejian A, Svitkin YV, Sukarieh R, M'Boutchou MN, Sonenberg N: Mammalian poly(A)-binding protein is a eukaryotic translation initiation factor, which acts via multiple mechanisms. Genes Dev. 2005, 19 (1): 104-113. 10.1101/gad.1262905.
Mili S, Shu HJ, Zhao Y, Pinol-Roma S: Distinct RNP complexes of shuttling hnRNP proteins with pre-mRNA and mRNA: candidate intermediates in formation and export of mRNA. Mol Cell Biol. 2001, 21 (21): 7307-7319. 10.1128/MCB.21.21.7307-7319.2001.
Kindler S, Wang H, Richter D, Tiedge H: RNA transport and local control of translation. Annu Rev Cell Dev Biol. 2005, 21: 223-245. 10.1146/annurev.cellbio.21.122303.120653.
Dreyfuss G, Kim VN, Kataoka N: Messenger-RNA-binding proteins and the messages they carry. Nat Rev Mol Cell Biol. 2002, 3 (3): 195-205. 10.1038/nrm760.
Kedersha N, Anderson P: Stress granules: sites of mRNA triage that regulate mRNA stability and translatability. Biochem Soc Trans. 2002, 30 (Pt 6): 963-969.
Eulalio A, Behm-Ansmant I, Izaurralde E: P bodies: at the crossroads of post-transcriptional pathways. Nat Rev Mol Cell Biol. 2007, 8 (1): 9-22. 10.1038/nrm2080.
Bevilacqua A, Ceriani MC, Capaccioli S, Nicolin A: Post-transcriptional regulation of gene expression by degradation of messenger RNAs. J Cell Physiol. 2003, 195 (3): 356-372. 10.1002/jcp.10272.
Meyer S, Temme C, Wahle E: Messenger RNA turnover in eukaryotes: pathways and enzymes. Crit Rev Biochem Mol Biol. 2004, 39 (4): 197-216. 10.1080/10409230490513991.
Richter JD, Sonenberg N: Regulation of cap-dependent translation by eIF4E inhibitory proteins. Nature. 2005, 433 (7025): 477-480. 10.1038/nature03205.
Sanchez-Velas N, Udofia I, Yu Z, Zapp M: hRIP, a cellular cofactor for Rev function, promotes release of HIV-1 RNAs from the perinuclear region. Genes & Dev. 2004, 18: 23-34. 10.1101/gad.1149704.
Fang J, Kubota S, Yang B, Zhou N, Zhang H, Godbout R, Pomerantz RJ: A DEAD box protein facilitates HIV-1 replication as a cellular co-factor of Rev. Virology. 2004, 330 (2): 471-480. 10.1016/j.virol.2004.09.039.
Yedavalli VS, Neuveut C, Chi YH, Kleiman L, Jeang KT: Requirement of DDX3 DEAD box RNA helicase for HIV-1 Rev-RRE export function. Cell. 2004, 119 (3): 381-392. 10.1016/j.cell.2004.09.029.
Pongoski J, Asai K, Cochrane A: Positive and Negative Modulation of Human Immunodeficiency Virus Type 1 Rev Function by cis and trans Regulators of Viral RNA Splicing. J Virol. 2002, 76 (10): 5108-5120. 10.1128/JVI.76.10.5108-5120.2002.
Hope TJ, Huang X, McDonald D, Parslow TG: Steroid-Receptor Fusion of the Human Immunodeficiency Virus Type 1 Rev Transactivator: Mapping of Cryptic Functions of the Arginine-Rich Motif. Proc Nat Acad Sci USA. 1990, 87: 7787-7791. 10.1073/pnas.87.19.7787.
Malim MH, Hauber J, Le S-Y, Maizel JV, Cullen BR: The HIV-1 rev trans-activator acts through a structured target sequence to activate nuclear export of unspliced viral mRNA. Nature. 1989, 338: 254-257. 10.1038/338254a0.
Seguin B, Staffa A, Cochrane A: Control of HIV-1 RNA Metabolism: The Role of Splice Sites and Intron Sequences in Unspliced Viral RNA Subcellular Distribution. J Virol. 1998, 72: 9503-9513.
Kriegler M: Gene Transfer and Expression: A Laboratory Manual. 1990, New York: Stockton Press, 1
Perkins A, Cochrane AW, Ruben SM, Rosen CA: Structural and functional characterization of the human immunodeficiency virus rev protein. J Acquir Immune Defic Syndr. 1989, 2 (3): 256-263.
Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987, 162: 156-159. 10.1016/0003-2697(87)90021-2.
Li Y, Bor YC, Misawa Y, Xue Y, Rekosh D, Hammarskjold ML: An intron with a constitutive transport element is retained in a Tap messenger RNA. Nature. 2006, 443 (7108): 234-237. 10.1038/nature05107.
Siomi MC, Zhang Y, Siomi H, Dreyfuss G: Specific sequences in the fragile X syndrome protein FMR1 and the FXR proteins mediate their binding to 60S ribosomal subunits and the interactions among them. Mol Cell Biol. 1996, 16 (7): 3825-3832.
KM and VS were supported by OHTN studentships. AC is the recipient of an OHTN scientist award. Studies were supported by an operating grant from CIHR. M. McLaren cloned Sam68Δ28ΔC. Antibodies were generously provided by N. Sonenberg, and H. Schaal.
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