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Figure 7 | Retrovirology

Figure 7

From: Selective translational repression of HIV-1 RNA by Sam68DeltaC occurs by altering PABP1 binding to unspliced viral RNA

Figure 7

Effect of Sam68ΔC mutants on protein expression from the HIV-1 provirus. 293T cells were transfected with the proviral clone pHxBruR-/RI- in the absence (pcDNA) or presence of the various Sam68ΔC mutants. Western blots on total cell lysates harvested from 293T cells transfected with HxBruR-/RI- and either pcDNA, Sam68ΔC or mutants thereof. p24 is expressed from the 9 kb, gp120 is expressed from the 4 kb, and Rev (top band) is expressed from the 2 kb class of proviral mRNA. Blots were reprobed with anti-myc to confirm protein expression and anti-tubulin to normalize for loading.

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Retrovirology

ISSN: 1742-4690

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