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Human APOBEC1 cytidine deaminase edits HBV DNA
Retrovirology volume 6, Article number: 96 (2009)
Retroviruses, hepadnaviruses, and some other retroelements are vulnerable to editing by single stranded DNA cytidine deaminases. Of the eleven human genes encoding such enzymes, eight have demonstrable enzymatic activity. Six of seven human APOBEC3 are able to hyperedit HBV DNA, frequently on both strands. Although human APOBEC1 (hA1) is not generally expressed in normal liver, hA1 can edit single stranded DNA in a variety of experimental assays. The possibility of ectopic expression of hA1 in vivo cannot be ruled out and interestingly, transgenic mice with A1 expressed under a liver specific promoter develop hepatocellular carcinoma. The impact of hA1 on HBV in tissue culture is varied with reports noting either reduced DNA synthesis or not, with cytidine deamination taking a low profile. We sought to examine the hA1 editing activity on replicating HBV. Using highly sensitive 3DPCR it was possible to show that hA1 edits the HBV minus DNA strand as efficiently as hA3G, considered the reference deaminase for HIV and HBV. The dinucleotide specificity of editing was unique among human cytidine deaminases providing a hallmark of use in a posteriori analyses of in vivo edited genomes. Analysis of sequences derived from the serum of two chronic carriers, indicated that hA1 explained only a small fraction of edited HBV genomes. By contrast, several human APOBEC3 deaminases were active including hA3G.
Despite being the prototypic human cytidine deaminase, human APOBEC1 (hA1) first identified in 1995 has been overshadowed by other paralogs, notably activation induced cytidine deaminase (AICDA) and the APOBEC3 gene cluster at ch22q13.1 [1–3]. Human A1 (hA1) edits a single cytidine residue in human apolipoprotein B (apoB) mRNA, a specificity that is conferred by its major interactor, ACF, its expression being confined to intestinal epithelial cells [4, 5]. By contrast, the mouse, rat, dog and horse A1s are expressed in the intestine and other organs including the liver [2, 6, 7]. This situation is probably due to an Alu insertion in a part of the hA1 gene inactivating a generalist promoter [8, 9]. RNA editing specificity can break down in rabbit A1 transgenic mice where hyperediting of the apoB mRNA was described  and subsequently noted in normal mouse intestinal tissues . For transgenic mice expressing the rabbit APOBEC1 gene under the control of a liver specific promoter, hepatic dysplasia and hepatocellular carcinomas were found . Whether this is due to RNA or DNA editing is unknown although in an E. coli DNA mutator assay, hA1 was highly mutagenic meaning that the latter cannot be ruled out . It turned out that human and mouse/rat A1 enzymes are not true orthologs in that the rodent enzymes can hyperedit both RNA and DNA, unlike hA1 that can only deaminate ssDNA . By contrast AICDA and the human APOBEC3 (hA3) show an exclusive single stranded DNA substrate specificity [14–27].
As retroviruses and hepadnaviruses replicate via a single stranded cDNA intermediate, it is not surprising that some are vulnerable to the effects of these cytidine deaminases if expressed in the target cell. While there is a huge literature on the interaction between human immunodeficiency virus (HIV) and the hA3 cluster of genes (the hA3 genes), most of them can also edit hepatitis B virus (HBV) DNA [28–33]. The role of A1 genes on retroviral replication is somewhat checkered. One report has shown that HBV replication is restricted by hA1 yet doesn't address the question of editing . By contrast, another study shows that the rat A1 deaminase hardly impacts HBV replication, even though a little cytidine deamination was found . Both hA1 and rat A1 impact HIV replication in single cycle growth assays . In the mouse A1 hardly restricts Friend murine leukemia virus replication, although using highly sensitive 3DPCR hypermutants were recovered from a small fraction of cultured cells or splenocytes from infected mice .
We sought to investigate the editing capacity of hA1 on HBV replication using 3DPCR [28, 31, 32]. An infectious molecular clone of HBV was transfected into quail QT6 cells with hA1 along with human APOBEC2 (hA2) and human APOBEC3G (hA3G) as negative and positive controls. QT6 cells were used so as to eliminate the endogenous APOBEC background typical of mammalian and human cell lines . At 72 h culture supernatants and cell lysates were recovered. Western blotting of whole cell lysates showed that all three APOBEC constructs were produced without obvious degradation (Figure 1A), the levels of hA1 and hA3G being particularly comparable. Using SYBR green quantification of HBV DNA, when normalized to the empty expression vector transfection control, hA1 and hA3G clearly impacted DNA replication (Figure 1B). PCR was performed on the HBV X gene using a conventional two-step procedure as previously described [28, 31]. Amplification performed using a 95°C denaturation temperature recovered HBV DNA in all samples, apart from the negative DNA control (Figure 1C). When 3DPCR was performed on these PCR products at the restricting temperature of 88.7°C, DNA was recovered only from the hA1 and hA3G transfections (Figure 1C). Cloning and sequencing of 24 clones identified so-called G->A hypermutants indicative of genetic editing of the minus DNA strand or cDNA, the frequency distribution of edited genomes per sequence being generally comparable (Figure 1D). No C->T hypermutants, indicative of plus strand editing, were recovered. The mutation matrices (Figure 1E) and editing frequencies were strictly comparable, hA1 32% (range 16-52%) while that for hA3G was 34% (range 25-41%). In short, hA1 is every bit as efficient as hA3G in editing HBV DNA.
As the dinucleotide context of editing is frequently a hallmark of different deaminases, for example hA3G shows a strong preference for CpC, while hA3H prefers TpC, the hA1 edited genomes were so analyzed. As can be seen from Figure 1F, hA1 showed a strong preference for TpC and a strong aversion for GpC. It was neutral with respect to ApC. All other hA3 deaminases (hA3s) either avoid GpC and ApC or are neutral (hA3A, hA3C, ). Hence, along with the preference for TpC, this feature is highly distinctive of hA1 editing.
Of course in vivo, one or more APOBEC deaminases could be operative, either in different HBV infected cells or even within the same cell. Hence global dinucleotide analyses of edited genomes from patients might be misleading. Comparative analysis of the dinucleotide contexts for individual in vivo hyperedited sequences with reference sets derived from transfections with individual hA1 or hA3 genes should help highlight those deaminases operative in vivo. The only exception would be if two different hA3 enzymes were packaged in the same virion. As can be seen plotting the number of edited TpC vs. CpC sites allows a clear distinction between hA1 and hA3G edited sequences (Figure 2A). The same is true for GpC vs. ApC (Figure 2B), although the number of deaminated bases is far fewer, meaning that the observation is a little less robust. The distinction between hA1 and hA3G could be emphasized by analyzing TpC+ApC (unique to hA1) vs. GpC+CpC (Figure 2C).
We have previously reported hA3 editing of HBV genomes from sera derived from two chronically infected patients with high viremia (> 109 DNA copies/ml, ). Single molecule analysis of these sequences showed that there was more overlap between the patient data "clouds" than with that of hA3G. Although the individual dinucleotide analyses might suggest that a few genomes are edited by hA1 in vivo (Figures 2D, E vs. 2A, B), the combined dinucleotide analysis (Figure 2C vs. 2F) showed no overlap with the hA1 edited sequence set. We have previously shown that the dinucleotide bias for individual hA3 deaminases varies with the base composition of the locus ; hence, it is not possible to extend the analysis to other edited regions of the HBV genome.
As ~20 hypermutated sequences were derived from each of the sera, the resolution of the argument is ~5%. Obviously with 10 times more patient sequences it might be able to find bona fide evidence of a little hA1 editing. By contrast, hA3G accounts for a sizeable fraction (79% and 58% respectively for patients 130.71 and 12763). Accordingly, other hA3 deaminases must be involved; yet given the similarities between the editing contexts of hA3A, hA3B and hA3C, it is not possible to be more precise. Furthermore, chronic hepatitis shows many facets; and although hA1 expression is confined to the intestinal epithelium and not the liver , its expression profile in a variety of different clinical presentations including highly inflamed cirrhotic tissue is not known.
As mentioned above, hA1 transgenic mice under the control of the liver specific apoE promoter presented with hepatic dysplasia and ultimately liver cancer; thus, the role of cytidine deamination remains open. Even though hA1 does not edit HBV at a high frequency, because hA1 can shuttle between the cytoplasm and nucleus, it is potentially a more likely pro-cancerous candidate than hA3G, which is strictly cytoplasmic.
Differential DNA denaturation PCR
APOBEC1 complementary factor
Human activation induced cytidine deaminase
hA1: Human apolipoprotein B editing complex protein 1
hA2: Human apolipoprotein B editing complex protein 2
hA3: Human apolipoprotein B editing complex protein 3
hA3A-H: Human apolipoprotein B editing complex protein 3 proteins A to H
Hepatitis B virus
Human Immunodeficiency virus type 1.
Jarmuz A, Chester A, Bayliss J, Gisbourne J, Dunham I, Scott J, Navaratnam N: An anthropoid-specific locus of orphan C to U RNA-editing enzymes on chromosome 22. Genomics. 2002, 79: 285-296. 10.1006/geno.2002.6718.
Lau PP, Zhu HJ, Baldini A, Charnsangavej C, Chan L: Dimeric structure of a human apolipoprotein B mRNA editing protein and cloning and chromosomal localization of its gene. Proc Natl Acad Sci USA. 1994, 91: 8522-8526. 10.1073/pnas.91.18.8522.
Teng B, Burant CF, Davidson NO: Molecular cloning of an apolipoprotein B messenger RNA editing protein. Science. 1993, 260: 1816-1819. 10.1126/science.8511591.
Chester A, Weinreb V, Carter CW, Navaratnam N: Optimization of apolipoprotein B mRNA editing by APOBEC1 apoenzyme and the role of its auxiliary factor, ACF. RNA. 2004, 10: 1399-1411. 10.1261/rna.7490704.
Maris C, Masse J, Chester A, Navaratnam N, Allain FH: NMR structure of the apoB mRNA stem-loop and its interaction with the C to U editing APOBEC1 complementary factor. RNA. 2005, 11: 173-186. 10.1261/rna.7190705.
Greeve J, Altkemper I, Dieterich JH, Greten H, Windler E: Apolipoprotein B mRNA editing in 12 different mammalian species: hepatic expression is reflected in low concentrations of apoB-containing plasma lipoproteins. J Lipid Res. 1993, 34: 1367-1383.
Nakamuta M, Oka K, Krushkal J, Kobayashi K, Yamamoto M, Li WH, Chan L: Alternative mRNA splicing and differential promoter utilization determine tissue-specific expression of the apolipoprotein B mRNA-editing protein (Apobec1) gene in mice. Structure and evolution of Apobec1 and related nucleoside/nucleotide deaminases. J Biol Chem. 1995, 270: 13042-13056. 10.1074/jbc.270.22.13042.
Greeve J, Axelos D, Welker S, Schipper M, Greten H: Distinct promoters induce APOBEC-1 expression in rat liver and intestine. Arterioscler Thromb Vasc Biol. 1998, 18: 1079-1092.
Hirano K, Min J, Funahashi T, Davidson NO: Cloning and characterization of the rat apobec-1 gene: a comparative analysis of gene structure and promoter usage in rat and mouse. J Lipid Res. 1997, 38: 1103-1119.
Innerarity TL, Boren J, Yamanaka S, Olofsson SO: Biosynthesis of apolipoprotein B48-containing lipoproteins. Regulation by novel post-transcriptional mechanisms. J Biol Chem. 1996, 271: 2353-2356. 10.1074/jbc.271.5.2353.
Petit V, Guetard D, Renard M, Keriel A, Sitbon M, Wain-Hobson S, Vartanian JP: Murine APOBEC1 is a powerful mutator of retroviral and cellular RNA in vitro and in vivo. J Mol Biol. 2009, 385: 65-78. 10.1016/j.jmb.2008.10.043.
Yamanaka S, Balestra ME, Ferrell LD, Fan J, Arnold KS, Taylor S, Taylor JM, Innerarity TL: Apolipoprotein B mRNA-editing protein induces hepatocellular carcinoma and dysplasia in transgenic animals. Proc Natl Acad Sci USA. 1995, 92: 8483-8487. 10.1073/pnas.92.18.8483.
Harris RS, Petersen-Mahrt SK, Neuberger MS: RNA editing enzyme APOBEC1 and some of its homologs can act as DNA mutators. Mol Cell. 2002, 10: 1247-1253. 10.1016/S1097-2765(02)00742-6.
Beale RC, Petersen-Mahrt SK, Watt IN, Harris RS, Rada C, Neuberger MS: Comparison of the differential context-dependence of DNA deamination by APOBEC enzymes: correlation with mutation spectra in vivo. J Mol Biol. 2004, 337: 585-596. 10.1016/j.jmb.2004.01.046.
Bishop KN, Holmes RK, Sheehy AM, Davidson NO, Cho SJ, Malim MH: Cytidine deamination of retroviral DNA by diverse APOBEC proteins. Curr Biol. 2004, 14: 1392-1396. 10.1016/j.cub.2004.06.057.
Chelico L, Pham P, Calabrese P, Goodman MF: APOBEC3G DNA deaminase acts processively 3' -> 5' on single-stranded DNA. Nat Struct Mol Biol. 2006, 13: 392-9. 10.1038/nsmb1086.
Chelico L, Pham P, Goodman MF: Stochastic properties of processive cytidine DNA deaminases AID and APOBEC3G. Phil Trans R Soc Lond B. 2009, 364: 583-593. 10.1098/rstb.2008.0195.
Harris RS, Bishop KN, Sheehy AM, Craig HM, Petersen-Mahrt SK, Watt IN, Neuberger MS, Malim MH: DNA deamination mediates innate immunity to retroviral infection. Cell. 2003, 113: 803-809. 10.1016/S0092-8674(03)00423-9.
Lecossier D, Bouchonnet F, Clavel F, Hance AJ: Hypermutation of HIV-1 DNA in the absence of the Vif protein. Science. 2003, 300: 1112-10.1126/science.1083338.
Liddament MT, Brown WL, Schumacher AJ, Harris RS: APOBEC3F properties and hypermutation preferences indicate activity against HIV-1 in vivo. Curr Biol. 2004, 14: 1385-1391. 10.1016/j.cub.2004.06.050.
Mangeat B, Turelli P, Caron G, Friedli M, Perrin L, Trono D: Broad antiretroviral defence by human APOBEC3G through lethal editing of nascent reverse transcripts. Nature. 2003, 424: 99-103. 10.1038/nature01709.
Mariani R, Chen D, Schrofelbauer B, Navarro F, Konig R, Bollman B, Munk C, Nymark-McMahon H, Landau NR: Species-specific exclusion of APOBEC3G from HIV-1 virions by Vif. Cell. 2003, 114: 21-31. 10.1016/S0092-8674(03)00515-4.
Shivarov V, Shinkura R, Honjo T: Dissociation of in vitro DNA deamination activity and physiological functions of AID mutants. Proc Natl Acad Sci USA. 2008, 105: 15866-15871. 10.1073/pnas.0806641105.
Suspène R, Sommer P, Henry M, Ferris S, Guetard D, Pochet S, Chester A, Navaratnam N, Wain-Hobson S, Vartanian JP: APOBEC3G is a single-stranded DNA cytidine deaminase and functions independently of HIV reverse transcriptase. Nucleic Acids Res. 2004, 32: 2421-2429. 10.1093/nar/gkh554.
Wiegand HL, Doehle BP, Bogerd HP, Cullen BR: A second human antiretroviral factor, APOBEC3F, is suppressed by the HIV-1 and HIV-2 Vif proteins. EMBO J. 2004, 23: 2451-2458. 10.1038/sj.emboj.7600246.
Yu Q, Konig R, Pillai S, Chiles K, Kearney M, Palmer S, Richman D, Coffin JM, Landau NR: Single-strand specificity of APOBEC3G accounts for minus-strand deamination of the HIV genome. Nat Struct Mol Biol. 2004, 11: 435-442. 10.1038/nsmb758.
Zhang H, Yang B, Pomerantz RJ, Zhang C, Arunachalam SC, Gao L: The cytidine deaminase CEM15 induces hypermutation in newly synthesized HIV-1 DNA. Nature. 2003, 424: 94-98. 10.1038/nature01707.
Henry M, Guetard D, Suspene R, Rusniok C, Wain-Hobson S, Vartanian JP: Genetic editing of HBV DNA by monodomain human APOBEC3 cytidine deaminases and the recombinant nature of APOBEC3G. PLoS ONE. 2009, 4: e4277-10.1371/journal.pone.0004277.
Noguchi C, Ishino H, Tsuge M, Fujimoto Y, Imamura M, Takahashi S, Chayama K: G to A hypermutation of hepatitis B virus. Hepatology. 2005, 41: 626-633. 10.1002/hep.20580.
Rosler C, Kock J, Kann M, Malim MH, Blum HE, Baumert TF, von Weizsacker F: APOBEC-mediated interference with hepadnavirus production. Hepatology. 2005, 42: 301-309. 10.1002/hep.20801.
Suspène R, Guétard D, Henry M, Sommer P, Wain-Hobson S, Vartanian JP: Extensive editing of both hepatitis B virus DNA strands by APOBEC3 cytidine deaminases in vitro and in vivo. Proc Natl Acad Sci USA. 2005, 102: 8321-8326. 10.1073/pnas.0408223102.
Suspène R, Henry M, Guillot S, Wain-Hobson S, Vartanian JP: Recovery of APOBEC3-edited human immunodeficiency virus G->A hypermutants by differential DNA denaturation PCR. J Gen Virol. 2005, 86: 125-129. 10.1099/vir.0.80426-0.
Turelli P, Mangeat B, Jost S, Vianin S, Trono D: Inhibition of hepatitis B virus replication by APOBEC3G. Science. 2004, 303: 1829-10.1126/science.1092066.
Jost S, Turelli P, Mangeat B, Protzer U, Trono D: Induction of antiviral cytidine deaminases does not explain the inhibition of hepatitis B virus replication by interferons. J Virol. 2007, 81: 10588-10596. 10.1128/JVI.02489-06.
Bishop KN, Holmes RK, Sheehy AM, Malim MH: APOBEC-mediated editing of viral RNA. Science. 2004, 305: 645-10.1126/science.1100658.
This work was supported by grants from the Institut Pasteur, ANRS and CNRS. MCG and RS were supported by a bursaries from CONACYT (Mexico) and ARC respectively.
The authors declare that they have no competing interests.
MCG, MH, DG and RS performed the work. SWH and JPV designed the study and wrote the paper.
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Gonzalez, M.C., Suspène, R., Henry, M. et al. Human APOBEC1 cytidine deaminase edits HBV DNA. Retrovirology 6, 96 (2009) doi:10.1186/1742-4690-6-96
- Cytidine Deaminase
- Friend Murine Leukemia Virus
- Cytidine Deamination
- Dinucleotide Context
- Liver Specific Promoter