Single molecule analysis of hyperedited HBV DNA. Based on the macroscopic trends identified in Figure 1F, mutated dinucleotide frequencies were plotted for individual minus strand hyperedited sequences. The units are numbers of NpC edited sites per clone. A-C) Data from HBV co-transfections with hA1 and hA3G (24 sequences each). D-F) Patient data from two individuals with high viremia chronic hepatitis (#12763, 19 sequences, ~109 DNA copies/mL; #130.71, 21 sequences, ~3 109 DNA copies/mL). For A to F, a few data points overlap; hence the number of points is less than the number of sequences. The diagonals represent the expected values assuming no dinucleotide preference. As the base composition differs between the sequences of the two HBV strains, dinucleotide composition varies and the expected values accordingly.