- Open Access
Tetraspanins regulate cell-to-cell transmission of HIV-1
Retrovirology volume 6, Article number: 64 (2009)
The presence of the tetraspanins CD9, CD63, CD81 and CD82 at HIV-1 budding sites, at the virological synapse (VS), and their enrichment in HIV-1 virions has been well-documented, but it remained unclear if these proteins play a role in the late phase of the viral replication cycle. Here we used overexpression and knockdown approaches to address this question.
Neither ablation of CD9, CD63 and/or CD81, nor overexpression of these tetraspanins was found to affect the efficiency of virus release. However, confirming recently reported data, tetraspanin overexpression in virus-producing cells resulted in the release of virions with substantially reduced infectivity. We also investigated the roles of these tetraspanins in cell-to-cell transmission of HIV-1. Overexpression of CD9 and CD63 led to reduced cell-to-cell transmission of this virus. Interestingly, in knockdown experiments we found that ablation of CD63, CD9 and/or CD81 had no effect on cell-free infectivity. However, knockdown of CD81, but not CD9 and CD63, enhanced productive particle transmission to target cells, suggesting additional roles for tetraspanins in the transmission process. Finally, tetraspanins were found to be downregulated in HIV-1-infected T lymphocytes, suggesting that HIV-1 modulates the levels of these proteins in order to maximize the efficiency of its transmission within the host.
Altogether, these results establish an active role of tetraspanins in HIV-1 producer cells.
Persistence of HIV-1 in infected individuals is a major public health problem. Despite great advances in anti-retroviral therapies, the virus cannot be completely eliminated once infection is established. One (of the many) potential explanation(s) for this failure of infected individuals to clear the virus is that its mode of spread does not allow components of the immune system to recognize and attack it appropriately. It is now well documented that HIV-1 can be transferred very efficiently from cell-to-cell, most likely upon induction of so-called virological synapses (VSs), sites of transient adhesion between infected (producer) and uninfected (target) cells [1–7]. Upon formation of the VS, viral budding is polarized towards the target cell, and the virus is thought to be released into the tight synaptic cleft where it appears to be at least partially protected from neutralizing antibodies .
Clearly, how the VS is formed and organized is an important question. Like immunological synapse (IS) formation, VS formation likely requires the concerted action of numerous cellular factors, several of which are also utilized for IS formation. Indeed it has been proposed that HIV-1 infection, followed by the expression of viral proteins, favors the formation of VSs at the expense of IS formation (e.g. , reviewed in ). However, we are only at a very early stage of understanding the relative distribution and function of viral and cellular elements during the formation, maintenance and disengagement of the VS. Specifically, while some studies show that VS formation and virus transfer is primarily driven by Env-CD4 interactions [2, 3, 10], other cellular components involved are only beginning to be unveiled.
Tetraspanins are a 33-member family of 4-span transmembrane proteins. They are thought to act as membrane organizers, selectively clustering proteins into microdomains in order for specific membrane-based processes to proceed [11, 12]. These processes include (among others) cell-cell fusion, cell adhesion, and cell motility (reviewed e.g. in [13, 14]). Interestingly, tetraspanins are recruited to sites of HIV-1 budding, as evidenced by their incorporation into viral particles [15, 16], as well as their clustering at budding sites in virus-producing cells, including the VS [17–22]. While several reports document involvement of these proteins in HIV-1 entry and possibly activation of newly infected cells [23–27], it remained unclear whether these proteins also play a functional role during the late stages of virus replication, specifically during the budding process [28–30]. We have recently shown that treatment of virus-producing HeLa cells with an anti-CD9 antibody reduces virus release . While this pointed towards a potential role of this tetraspanin in HIV-1 budding, it appears now more likely that this treatment, which results in the clustering not only of CD9 but also of other tetraspanins and of all the viral structural components, simply redirects particle formation towards cell-cell contact sites, thus reducing the overall area through which progeny virus can exit from cells .
Here, we show that tetraspanins in producer cells, rather than acting as budding co-factors, regulate cell-free virus infectivity (confirming recent data by the Koyanagi laboratory ) and cell-to-cell transmission. Further, we provide evidence that HIV-1 modulates the levels of tetraspanins in producer cells in order to maximize the efficiency of its dissemination. Altogether, our findings identify new cellular players involved in the late stages of viral replication, specifically in the transfer of viral particles from cell-to-cell, a process that is crucial for HIV-1 dissemination in infected individuals.
Cell culture, plasmids, and antibodies
The following reagents were obtained through the NIH AIDS Research and Reference Reagent Program, Division of AIDS, NIAID, NIH: TZM-bl cells from Dr. John C. Kappes, Dr. Xiaoyun Wu and Tranzyme Inc, Jurkat Clone E6-1 cells from Dr. Arthur Weiss, CEM.NKR-CCR5-Luc (referred to as CEM-Luc throughout) cells from Drs. John Moore and Catherine Spenlehauer, CEM-GFP cells from Dr. Jacques Corbeil; as well as reverse transcriptase inhibitors Zidovudine and Efavirenz, and the following antibodies: monoclonal antibody to HIV-1 p24 (AG3.0) from Dr. Jonathan Allan, HIVIG from NABI and NHLBI, HIV-1 p24 Hybridoma (183-H12-5C) from Dr. Bruce Chesebro.
HeLa and TZM-bl cells were maintained in DMEM with 10% FBS. All T cell lines were maintained in RPMI with 10% FBS. Media for CEM-GFP and CEM.NKR-CCR5-Luc were supplemented with 0.5 and 0.8 mg/ml G418, respectively.
The following proviral plasmids were used: pNL4-3, pNL4-3deltaNef (provided by Dr. John Guatelli, UCSD), HIV-i-GFP (pNL4-3 containing a MA-GFP fusion, and an additional protease cleavage site between MA and GFP) , and HXB2 (provided by Dr. Clarisse Berlioz-Torrent, Institut Cochin, France). Previously described , tetraspanin and L6 expression plasmids (in pCMV-Sport6 vector) were provided by Dr. Koyanagi, (Kyoto University, Japan). The FG12 shRNA delivery vector system was developed in Dr. David Baltimore's laboratory  and was obtained via the AddGene service (addgene.org).
ShRNA sequences were based on previously described siRNA sequences:  for CD9 and CD81, and Ambion's pre-designed CD63 (ID:10412). The scrambled shRNA sequence was generated by randomly scrambling the siRNA sequence used for CD63 silencing. Using NCBI BLAST, the scrambled sequence showed no significant homology with the human genome.
The lentiviral shRNA system used has been previously described , and cloning and other procedures were carried out essentially as described. Briefly, stock lentiviruses were produced by transfecting 293T cells with the FG12shRNA plasmids, pVSVG and packaging plasmid pDeltaR8.2. Supernatants were concentrated 200 fold by centrifugation at 50,000 g for 2 hours, then stored at -80°C. Titer was determined by infecting either HeLa or Jurkat cells with serial dilutions of virus, then determining %GFP positive cells by flow cytometry. Infections were done in the presence of 8 μg/ml polybrene (HeLa) or 10 μg/ml DEAE-dextran (Jurkat) to enhance transduction efficiency. Knockdown efficiency was confirmed by Western blot and flow cytometry, using the following antibodies: mouse anti-CD63, clone H5C6 (Developmental Studies Hybridoma Bank at the University of Iowa), mouse anti-CD9, clone K41 (Bachem), mouse anti-CD81, clone JS-81 (BD Biosciences), and mouse anti-GAPDH (Abcam). GAPDH was used as a loading control. Lysates were analyzed for protein content using the Coomassie Plus reagent (Pierce), and equal amounts of protein (5–10 μg) were loaded for each lane.
HIV-1 release assays
HeLa cells were seeded at a density of 0.3 × 106 cells per well in 6 well plates (day 0). On day 1, cells were transduced with lentiviruses carrying shRNA at MOI = 8 overnight in the presence of 8 μg/ml Polybrene (Sigma). On day 3, cells were transfected with pNL4-3, using Lipofectamine2000 following manufacturer's instructions (Invitrogen). On day 5, supernatant containing released virus was centrifuged at 3,000 g, 10 minutes, to pre-clear, followed by 2 hours at 16,000 g through a 20% sucrose cushion. Viral pellets were re-suspended in TNE lysis buffer (10 mM Tris pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% Triton-X100). Cells were lysed, centrifuged at 16,000 g for 10 minutes and were loaded for SDS-PAGE and Western blot together with released virus. AG3.0 anti-p24 antibody was used to detect Gag. Cell lysates and supernatants were also analyzed for p24 content by p24 antigen capture ELISA as described , with the following modifications: HIV-IG was used as an antigen detection antibody, followed by donkey anti-human HRP-conjugated antibody (Jackson ImmunoResearch) and a TMB substrate kit was used for colorimetric detection (Pierce).
Cell-free infectivity assay
Supernatants from virus-expressing HeLa cells were pre-cleared at 3,200 g for 10 minutes, then assayed for p24 content using a home-made ELISA kit (see above). TZM-bl reporter cells, which express beta-galactosidase and luciferase under the control of the HIV LTR, were plated in 96-well plates (10,000 cells/well) one day prior to infection. 0.2 – 0.05 ng of p24 in 100 μl of media containing 20 μg/ml DEAE-dextran (to enhance infection) was used per well. 48 hours later, media was removed, cells were washed and 50 μl/well of All-in-One mammalian beta-galactosidase reagent (Pierce, USA) was added. The plate was incubated at 37°C for 5–30 minutes, and absorbance was read at 405 nm in a microplate reader. The absorbance was normalized by the p24 input for different dilutions and further normalized to the mock condition for that experiment (set to 1).
Cell-to-cell transmission assay
50,000 HeLa cells/well were plated in 24 well plates on Day 0. On Day 1, cells were co-transfected with tetraspanin expression plasmids and the appropriate proviral plasmid at 1:2 ratio. Transfections were performed in quadruplicate. On Day 2, media was removed, and target cells (either 0.2 million (mio) CEM-GFP or 0.4 mio CEM-Luc cells) were added to 2 out of the 4 quadruplicate wells. On Day 3, non-adherent target cells were removed and transferred to a new plate. Supernatants from replicate wells without target cells were harvested and assayed for p24 content using a home-made ELISA kit (used for normalization, see below). These supernatants were also used in infectivity assays (see above). On Day 4, non-adherent target cells were removed from the second plate. CEM-GFP cells were fixed with 4% paraformaldehyde (PFA) and analyzed by flow cytometry, the readout being %GFP positive cells. CEM-Luc cells were lysed in TNE Lysis buffer, then assayed for luciferase activity using the Bright-Glo Luciferase Assay System (Promega, USA). Both readouts for were normalized by p24 content of the replicate wells without target cells, and normalized to the mock condition.
Cell-to-cell transmission assays with tetraspanin knockdowns were performed essentially as described above, with the following differences. HeLa cells were infected with lentiviruses carrying shRNA at an MOI = 8 2 days prior to transfection with pNL4-3, and the rest of the assay was carried out using CEM-Luc target cells as described above.
When Jurkat cells were used as producers, these were simultaneously infected with NL4-3 at an approximate MOI of 0.2 and with lentiviruses carrying shRNA at MOI = 8. 3 days post-infection, these cells were counted, 0.25 mio cells were mixed with 0.25 mio CEM-Luc target cells, and co-cultured for 3 days, or cultured without target cells. The cells were then lysed and assayed for luciferase activity as above. This readout was normalized by p24 output of producer cells without target cells to correct for potential cell death of producer cells due to shRNA treatment.
Latrunculin B treatment
The cell-to-cell transmission assay was carried out as above, with the following modifications. Producer HeLa cells were pretreated with Latrunculin B for 1 hour prior to addition of CEM-GFP target cells. Producer and target cells were co-cultured in the presence of Latrunculin B for 24 hours, target cells were removed and washed, then cultured for another 24 hours and analyzed by flow cytometry.
Jurkat cells were infected with stock NL4-3 virus at an approximate MOI = 0.1. 48 hours later, cells were allowed to adhere to Cell-Tak (BD Biosciences) coated coverslips in Mattek dishes (Mattek Corporation, USA) for 1–2 hours, then fixed and stained with tetraspanin and Gag antibodies. The following antibodies were used: mouse anti-CD63, clone H5C6 (Developmental Studies Hybridoma Bank at the University of Iowa), mouse anti-CD81, clone JS-81 (BD Biosciences), anti-p6 Gag rabbit serum (gift of Dr. David Ott, NCI Frederick). The cells were then incubated with appropriate AlexaFluor488 and AlexaFluor594 secondary antibodies (Invitrogen, USA). The slides were examined on a Delta Vision Workstation (DV base 3/3.5, Nikon Eclipse TE200 epifluorescence microscope fitted with an automated stage, Applied Precision Inc., Issaquah, WA, USA) and images were captured in z-series with a CCD digital camera (CoolSnap HQ). Out of focus light was digitally reassigned using Softworx deconvolution software (Applied Precision Inc.). Relative tetraspanin signal intensity was quantified using Volocity software (Improvision), using the classifier module set to 1 SD below the mean intensity (and no upper limit) to define the regions of interest, then analyzing their mean intensity. The background fluorescence intensity was also determined by selecting an area of a micrograph devoid of cells, and this value was subtracted from the mean intensity value determined for cells. At least 20 cells were analyzed for each condition.
Visualization of VS formation
Microscopy experiments were performed essentially as above, with the following modifications. Uninfected target Jurkat cells were labeled with CMAC CellTracker Blue (Invitrogen) according to manufacturer's instructions, then mixed with an equal number of infected producer cells. The cell mixture was incubated on Cell-Tak-coated coverslips for 2 hours at 37°C, then fixed, stained and visualized as above.
A two-tailed, unequal variance Student's t-test was used to determine significance throughout. A p value less than 0.05 was considered significant.
CD9, CD63 and CD81 are dispensable for HIV-1 budding
Because tetraspanins are known to enhance or repress the function of peripheral and integral membrane proteins and because of their well-documented presence at HIV-1 exit sites, we tested if they act as budding co-factors. In order to achieve efficient silencing of tetraspanins, a lentivirus-based shRNA delivery system was employed. Using this system, at high MOI approximately 99% of the cells were transduced, and levels of CD63, CD9 and CD81 could be reduced dramatically (see Fig. 1A). Note that CD63 shows up as a smear on the Western blot, due to heavy glycosylation of this protein. Production of viral proteins and particle release in tetraspanin knock-down cells was compared to protein production and virus shedding in control cells, using Western blot and p24 ELISA (Figures 1A and 1B). As documented in Fig. 1, all three tetraspanins tested were dispensable for HIV-1 assembly and budding, as ablation of any one or all three of them did not significantly affect the efficiency of release (p > 0.05). Consistent with a recent report , overexpression of these three tetraspanins, which augmented their surface presence 5, 8, and 7 fold for CD9, CD63, and CD81, respectively, (see  for flow cytometry analysis), also did not affect particle release (Fig. 1C), although we noticed that CD81 overexpression decreased the synthesis of viral proteins (data not shown). Because of this variability in viral output, transmission and infectivity assay data were always corrected by the amount of virus released (see Methods).
Overexpression of tetraspanins in HIV-1 producer cells reduces the infectivity of cell-free virus as well as cell-to-cell transmission of virions to target cells
Tetraspanins clearly are enriched in HIV-1 particles (for a recent review, ); yet as shown above, their presence at the viral exit site is not required for particle formation and release. We thus set out to test if the virus benefits from the presence of these membrane proteins once they are incorporated into virions. Contrary to our expectation, however, our initial data (not shown) clearly demonstrated that this is not the case, and indeed while work for this report was in progress, a study by Koyanagi and colleagues showed that overexpression of CD9, CD63, CD81 and other tetraspanins in virus-producing cells resulted in production of virions with considerably reduced infectivity . As documented in Fig. 2, similar results were obtained in our system, which we adapted to use the same set of expression vectors used by the Koyanagi laboratory, in order to be able to directly compare the two studies. Thus, HeLa cells were co-transfected with the proviral expression plasmid pNL4-3 and tetraspanin expression plasmids. Cell-free supernatants from these producer cells that contained viral particles (equal amounts of p24 for each condition, normalized by p24 ELISA) were then used to infect TZM-bl cells, which contain the beta-galactosidase reporter gene controlled by the HIV LTR . Co-transfection of HeLa cells with the proviral construct and with tetraspanin expressors, particularly with a CD63 expressor or with a plasmid that expressed a CD63 which lacks part of its internalization signal/lysosomal sorting motif (CD63delL), led to increased levels of tetraspanins incorporated into released virions (Fig. 2A), and this markedly reduced the infectivity of the virus (Fig. 2B). In contrast, co-transfection of a plasmid that expressed L6, a tetraspanin-related protein that is situated within the same plasma membrane microdomains , had no effect on the infectivity of the viral particles.
Reduced infectivity of cell-free virus may not necessarily translate into similarly reduced cell-to-cell transmission, since even particles with reduced affinity for their receptors, for example, may still successfully enter cells if they are shed into the cleft of the VS (i.e. directly next to the target cell). We thus sought to determine if the tetraspanin overexpression also negatively affects the infectivity of the virus in a cell-to-cell transmission system.
Fig. 3A outlines the assay that was set up to monitor productive cell-to-cell transmission of HIV-1. In this system, HIV-1-producing HeLa cells are co-cultured with indicator CEM-GFP T lymphocytes, which express GFP under the control of the HIV LTR . After 24 hours of co-culture, the target CEM-GFP cells were gently resuspended and removed, leaving behind adherent producer cells as well as producer-target syncytia, which were also adherent. Further, larger cells (potentially syncytia or detached HeLa cells) were excluded from the read-out of the assay based on side and forward scatter characteristics during the subsequent flow cytometry analysis. After another 24 hours of culture, the cells were again resuspended and removed from the culture flask, again leaving behind any adherent cells; they were then fixed and analyzed for GFP expression by flow cytometry. Importantly, we knew that most of the infection of the reporter cells resulted from their direct contact with producer cells, because hardly any productive infection could be detected if the target cells were incubated with equivalent amounts of cell-free virus (Fig. 3B). Further, HIV-1-producing HeLa cells formed VS-like contacts with CEM target cells, and cell-to-cell transmission could be blocked using Latrunculin B to depolymerize actin, as previously described in the T cell-T cell scenario [2, 3] (see Figures 3C and 3D). Finally, this cell-to-cell transfer could be almost completely blocked by adding reverse transcriptase inhibitors (RTIs) AZT and Efavirenz (EFV) simultaneously with the addition of target cells (data not shown), demonstrating that reporter GFP expression was not a result of cell-cell fusion, which would be insensitive to RTIs, but rather would be the result of viral integration and productive infection.
As shown in Fig. 3E, using this cell-to-cell transmission assay, we found that over-expression of CD63 and to a lesser extent CD9, but not CD81, in producer cells, reduced productive virus transfer to target cells. The magnitude of the transmission reduction by CD63 overexpression was roughly half of the effect on cell-free virus infectivity. As measured for the infectivity of cell-free virus, overexpression of L6 had no effect on cell-to-cell virus transfer.
Since HIV-1 accessory proteins Nef and Vpu are well-documented modulators of viral infectivity and release, respectively, we also tested if their presence was required for the suppression of cell-to-cell transfer by CD63 overexpression. HeLa cells were co-transfected with a CD63 expressor plasmid and either with pNL4-3deltaNef (NLdelNef) which gives rise to the production of virus that lacks Nef, or with an HXB2 proviral plasmid, which leads to the expression of HIV-1 lacking both Nef and Vpu. Fig. 4 documents that CD63 overexpression suppressed cell-to-cell transmission of both viruses, indicating that Nef and Vpu are dispensable for the reduced cell-to-cell transmission caused by this tetraspanin.
Knockdown of CD81 enhances cell-to-cell transmission of HIV-1, but not the infectivity of cell-free virus
The results shown in Figures 2 and 3 suggest that tetraspanins may play a restrictive role in HIV-1 replication. We thus hypothesized that knockdown of these host factors would enhance virion infectivity and cell-to-cell transmission. However, ablation of CD9, CD63 or CD81 in HIV-1-producing HeLa cells, either alone or pair wise, or even simultaneous knockdown of all three tetraspanins, had no effect on cell-free virus infectivity (Fig. 5A).
To measure cell-to-cell transmission initiated by tetraspanin-depleted producer cells, we had to modify the assay shown in Fig. 3 because the lentiviral system for shRNA delivery uses GFP as an indicator of transduction. Instead of CEM-GFP cells, we used CEM.NKR-CCR5-Luc (CEM-Luc) cells, which express luciferase under the control of the LTR , as targets. Surprisingly, only the knockdown of CD81, but not the ablation of the other tetraspanins, enhanced cell-to-cell transmission of HIV-1 (Fig. 5B). However, since this effect was not paralleled by a significant increase in the entry of cell-free virus into target cells (p > 0.05), as shown in Fig. 5A, we concluded that CD81 knockdown in producer cells must affect another step in cell-to-cell transmission of HIV-1.
Importantly, the lentivirus-based shRNA knockdown system allowed us to also assess the effects of tetraspanin ablation in T cells. Jurkat T lymphocytes were co-infected with high doses of NL4-3 virus and lentiviruses carrying shRNA against CD63 and CD81. CD9 knockdown was not performed, since E6-1 Jurkat cells express very low amounts of this protein, similar to primary CD4+ T cells . These producer cells were then co-cultured with CEM-Luc target cells to allow for cell-to-cell transmission of virus. As in the HeLa system, knockdown of CD81 was found to enhance the cell-to-cell transmission of HIV-1 (Fig. 5C), without increasing cell-free virus infectivity (data not shown). Because this version of the transmission assay does not allow one to specifically exclude syncytia events, these experiments were also performed in the presence of EFV, to allow for syncytia formation but not successful transmission. As in previous experiments, EFV abolished most of the reporter activity, and the residual reporter activity did not change significantly in either of the knockdown conditions (data not shown).
Tetraspanins are downregulated in acutely infected lymphocytes
HIV-1 has been well documented to downregulate various cellular factors that are detrimental to its replication, such as CD4 or MHC Class I (reviewed e.g. in ). Since CD63, CD9, and CD81 appear to play partially restrictive roles in HIV-1 replication by reducing the infectivity of cell-free virions and also by inhibiting cell-to-cell transmission, it seemed plausible that the virus would adapt to overcome this restriction. Indeed, in cultures of infected Jurkat lymphocytes lower levels of CD81 could be readily observed by fluorescence microscopy analysis of newly infected cells expressing high amounts of Gag (Fig. 6A and 6B). A similar downregulation could be observed by quantitative Western blot in HIV-1-infected Jurkat cells (Fig. 6C). We also measured similar downregulation of CD82, another tetraspanin that is abundantly expressed in T lymphocytes (data not shown), and of CD9 and CD63, although these were more difficult to analyze, particularly in Jurkat sub-clones that have low endogenous levels of these two tetraspanins (data not shown). However, no tetraspanin downregulation was detected in provirus-transfected HeLa cells (data not shown). Finally, although the levels of tetraspanins were reduced in HIV-1-infected T cells, their recruitment to and their concentration at the VS were still readily detectable (Fig. 6D for CD81, data not shown for CD9 and CD63), suggesting that these proteins are not eliminated completely, and may still carry out certain functions (e.g. cell-cell fusion prevention, ).
While tetraspanins have been well-documented to be involved in various steps of the HIV-1 replication cycle, until recently only evidence for their functional involvement in potential target cells and in newly infected cells has been presented. Here, we provide data which establish tetraspanins also as regulators of HIV-1 spread through their presence in HIV-1-producing cells.
Despite the enrichment of CD63, CD9 and CD81 at HIV-1 budding sites, their knockdown did not affect the efficiency of HIV-1 release from HeLa cells. This suggests that these proteins do not act as budding factors for HIV-1, although we cannot formally exclude the possibility of compensation by other functionally redundant tetraspanins (e.g. CD82, CD151, etc). These results are in line with findings of the Marsh group, who reported that knockdown of CD63 in macrophages did not alter the efficiency of viral release, regardless of whether the knockdown was done prior to or before infection , though another recent study reported that CD63 knockdown after HIV-1 infection of macrophages reduced the amount of virus released from these cells . However, in the latter study, it remained unclear exactly which step was inhibited by CD63 ablation, as the amounts of released particles apparently were not corrected by the levels of cell-associated viral proteins. Further, another recent study utilizing a chronically infected T-cell line reported that knockdown of CD81 or treatment with an anti-CD81 antibody reduced viral release . It is possible that this effect may be dependent on the cell type, and the potential release functions of tetraspanins may not be recapitulated in the HeLa cell system.
Overexpression of CD63, CD9 and CD81 repressed cell-free virus infectivity. Similar data were recently published by Koyanagi and colleagues , although in our study the magnitude of the repression appears to vary more between different tetraspanins, the most potent being CD63. However, while it seems likely that specific tetraspanins act differently, we cannot exclude that this effect simply depends on endogenous levels of the tetraspanin at the surface in producer cells, with CD63 displaying the lowest surface fraction of the tetraspanins analyzed in the cells used in our analysis. Koyanagi and colleagues also showed that the inhibition of infectivity was not due to altered Gag or Env processing or Env incorporation, and occurred specifically at the virus-cell fusion stage of entry. Consistent with the latter finding, we observed that cell-cell fusion driven by Env is also inhibited by tetraspanin overexpression .
As shown in Fig. 3, overexpression of CD63, and to a lesser extent of CD9, also inhibited cell-to-cell transmission of HIV-1, suggesting that reduced infectivity of virus is capable of reducing its cell-to-cell transmission. The magnitude of this repression was about half of the effect on cell-free infectivity, thus the effects on cell-free infectivity are not exactly matched in cell-to-cell transmission. It is quite possible that tetraspanin overexpression, in addition to suppressing infectivity, could also have other, potentially positive effects on transmission, e.g. preventing fusion of target and producer cells, and thus preserving productive VSs . We have measured syncytia formation directly in our cell-to-cell transmission assay and found that while overexpression of CD63 repressed syncytia formation and transmission with similar efficiency, syncytia formation was relatively rare, occurring approximately once per 10 successful transmission events (data not shown), explaining why no obvious positive effect of syncytia repression was observed in our assays. Interestingly, overexpression of a different tetraspanin, CD82, was also shown to inhibit cell-to-cell transmission of another human retrovirus, HTLV-I, while also inhibiting syncytia formation . CD82 and CD81 were found to associate with HTLV-I Env and/or Gag protein, suggesting that tetraspanins are part of a common pathway of fusion and transmission regulation for different retroviruses [43–45].
The fact that knockdown of CD63, CD9 and CD81 failed to increase cell-free infectivity, although in agreement with previously published data [30, 33], is surprising, given that overexpression of these proteins reduced infectivity considerably. The most parsimonious explanation for this finding may be that the endogenous levels of these proteins in producer HeLa cells or acutely infected T cells are too low to effectively suppress infectivity. In fact, we have observed that uninfected lymphocytes have a relatively higher surface density of some tetraspanins, such as CD81 and CD82 (data not shown), and this may explain why the virus would downregulate these proteins to a level that is optimal for its infectivity, and yet is not too low to prevent syncytia formation (discussed in more detail below). Consistent with this, we have observed that virus-producing HeLa cells do not exhibit any appreciable tetraspanin downregulation (data not shown). Also, downregulation of tetraspanins in producer HeLa cells does in fact enhance syncytia formation . Finally, Muriaux and colleagues reported that CD81 knockdown in chronically infected T cells reduced infectivity of released virus . As above, we speculate that the levels of CD81 (and other tetraspanins) in these cells are higher than in HeLa cells or acutely infected or activated T cells, and this is supported by the findings of Koyanagi and colleagues .
Also consistent with the idea that the levels of CD9 and CD63 in HeLa cells are relatively low, downregulation of these two tetraspanins does not alter cell-to-cell transmission of HIV-1. Intriguingly though, knockdown of CD81 enhanced cell-to-cell virus transmission without affecting infectivity. This suggests that CD81 may play a role at a different step in the transmission process. Since this tetraspanin has been shown to be involved in cell-cell adhesion and cell migration, in addition to cell-cell fusion, it is possible that it affects the frequency or the duration of VS formation. Future studies will help to dissect exactly which step CD81 is involved in.
We found that tetraspanins are downregulated in infected lymphocytes, suggesting that HIV-1 may be actively lowering the levels of these proteins. This is in disagreement with data published by Sattentau and colleagues, who did not find a difference in CD63, CD9 and CD81 levels between infected and non-infected lymphocytes . However, in the aforementioned study, viral proteins were not directly measured together with tetraspanin levels. In our experiments, we observed that tetraspanin expression was quite variable in infected cultures, and clear-cut downregulation was only observed in cells expressing relatively high levels of Gag. Interestingly, a similar downregulation of tetraspanins was observed in PHA/PMA-stimulated lymphocytes chronically infected with HIV-1, which also correlated with increased virus production and enhanced virion infectivity . Intriguingly, enhancement of virion infectivity by HIV-1 Nef in CD4-negative cells has been recently shown to be dependent on the clathrin/dynamin2-dependent pathway . It is tempting to speculate that HIV-1 Nef may be involved in downregulating tetraspanins from the plasma membrane, preventing their incorporation into virions and thus enhancing virion infectivity. Another possibility is that tetraspanins are simply shed in the released virions, as a consequence of their incorporation into virus particles, although experiments performed in our laboratory indicate that tetraspanin downregulation does not correlate with the amount of released virus (M.L., D.K. and M.T., unpublished data).
Overall then, are tetraspanins restriction factors for HIV-1? Given that tetraspanins appear to reduce virus infectivity and cell-to-cell transmission, and that the virus downregulates these proteins, it is tempting to speculate that they indeed hinder HIV-1 replication. However, since tetraspanins also play a positive role, by preventing syncytia formation , and thus likely preserving the VS, it seems likely that the most beneficial scenario for the virus is to achieve a balance between enhanced infectivity and low syncytia formation. Consistent with this, we observed only a partial downregulation of tetraspanins in infected cells, and tetraspanins were still recruited to the VS (although at presumably lower levels, hence allowing for the enhancement of infectivity), where they could prevent cell-cell fusion. Further, while our current cell-to-cell transmission assays indicate a restrictive role for tetraspanins, this may not be the case in vivo, where fusion prevention may potentially be more important than infectivity enhancement. As discussed above, in our transmission assay, syncytia formation is a relatively rare event, occurring in roughly once per 10 successful transmission events (using HeLa cells as producers); thus a reduction in syncytia formation (e.g. by tetraspanin overexpression) would result in a very modest transmission enhancement. In vivo however, e.g. when the virus replicates in lymph nodes, where the cells are much more densely packed, there is less opportunity for producer and target cells to separate after VS formation and virus transmission, and the effects of impaired fusion regulation, if quantifiable, might become evident. Finally, another limitation of our transmission assays is that they measure the effect of tetraspanins on a single transmission step. However, multi-step transmission assays (e.g. a spreading infection in a lymphocyte culture), become very difficult to interpret, because tetraspanins, as mentioned before, also play a role in target cells.
Altogether, our results provide evidence for an active role of tetraspanins in cell-to-cell transmission of HIV-1, a process that may be crucial for virus spread in vivo and one which is only beginning to be understood. Because individual tetraspanins are not essential for host cell survival, they may provide an avenue for therapeutic intervention with the virus life cycle.
human immunodeficiency virus type 1
green fluorescent protein
HIV-1 envelope glycoprotein
major histocompatibility complex
standard error of the mean
small hairpin RNA.
Ruggiero E, Bona R, Muratori C, Federico M: Virological consequences of early events following cell-cell contact between human immunodeficiency virus type 1-infected and uninfected CD4+ cells. J Virol. 2008, 82: 7773-7789. 10.1128/JVI.00695-08.
Jolly C, Kashefi K, Hollinshead M, Sattentau QJ: HIV-1 Cell to Cell Transfer across an Env-induced, Actin-dependent Synapse. J Exp Med. 2004, 199: 283-293. 10.1084/jem.20030648.
Chen P, Hubner W, Spinelli MA, Chen BK: Predominant mode of human immunodeficiency virus transfer between T cells is mediated by sustained Env-dependent neutralization-resistant virological synapses. J Virol. 2007, 81: 12582-12595. 10.1128/JVI.00381-07.
Sourisseau M, Sol-Foulon N, Porrot F, Blanchet F, Schwartz O: Inefficient human immunodeficiency virus replication in mobile lymphocytes. J Virol. 2007, 81: 1000-1012. 10.1128/JVI.01629-06.
Phillips DM: The role of cell-to-cell transmission in HIV infection. Aids. 1994, 8: 719-731. 10.1097/00002030-199405000-00033.
Groot F, Welsch S, Sattentau QJ: Efficient HIV-1 transmission from macrophages to T cells across transient virological synapses. Blood. 2008, 111: 4660-4663. 10.1182/blood-2007-12-130070.
Gousset K, Ablan SD, Coren LV, Ono A, Soheilian F, Nagashima K, Ott DE, Freed EO: Real-time visualization of HIV-1 GAG trafficking in infected macrophages. PLoS Pathog. 2008, 4: e1000015-10.1371/journal.ppat.1000015.
Thoulouze MI, Sol-Foulon N, Blanchet F, Dautry-Varsat A, Schwartz O, Alcover A: Human immunodeficiency virus type-1 infection impairs the formation of the immunological synapse. Immunity. 2006, 24: 547-561. 10.1016/j.immuni.2006.02.016.
Fackler OT, Alcover A, Schwartz O: Modulation of the immunological synapse: a key to HIV-1 pathogenesis?. Nat Rev Immunol. 2007, 7: 310-317. 10.1038/nri2041.
Massanella M, Puigdomenech I, Cabrera C, Fernandez-Figueras MT, Aucher A, Gaibelet G, Hudrisier D, Garcia E, Bofill M, Clotet B, Blanco J: Antigp41 antibodies fail to block early events of virological synapses but inhibit HIV spread between T cells. Aids. 2009, 23: 183-188. 10.1097/QAD.0b013e32831ef1a3.
Wright MD, Moseley GW, van Spriel AB: Tetraspanin microdomains in immune cell signalling and malignant disease. Tissue Antigens. 2004, 64: 533-542. 10.1111/j.1399-0039.2004.00321.x.
Levy S, Shoham T: Protein-protein interactions in the tetraspanin web. Physiology (Bethesda). 2005, 20: 218-224.
Hemler ME: Tetraspanin proteins mediate cellular penetration, invasion, and fusion events and define a novel type of membrane microdomain. Annu Rev Cell Dev Biol. 2003, 19: 397-422. 10.1146/annurev.cellbio.19.111301.153609.
Zoller M: Tetraspanins: push and pull in suppressing and promoting metastasis. Nat Rev Cancer. 2009, 9: 40-55. 10.1038/nrc2543.
Orentas RJ, Hildreth JE: Association of host cell surface adhesion receptors and other membrane proteins with HIV and SIV. AIDS Res Hum Retroviruses. 1993, 9: 1157-1165. 10.1089/aid.1993.9.1157.
Ott DE: Cellular proteins detected in HIV-1. Rev Med Virol. 2008, 18: 159-175. 10.1002/rmv.570.
Welsch S, Keppler OT, Habermann A, Allespach I, Krijnse-Locker J, Krausslich HG: HIV-1 Buds Predominantly at the Plasma Membrane of Primary Human Macrophages. PLoS Pathog. 2007, 3: e36-10.1371/journal.ppat.0030036.
Nydegger S, Khurana S, Krementsov DN, Foti M, Thali M: Mapping of tetraspanin-enriched microdomains that can function as gateways for HIV-1. J Cell Biol. 2006, 173: 795-807. 10.1083/jcb.200508165.
Turville SG, Aravantinou M, Stossel H, Romani N, Robbiani M: Resolution of de novo HIV production and trafficking in immature dendritic cells. Nat Methods. 2008, 5: 75-85. 10.1038/nmeth1137.
Pelchen-Matthews A, Kramer B, Marsh M: Infectious HIV-1 assembles in late endosomes in primary macrophages. J Cell Biol. 2003, 162: 443-455. 10.1083/jcb.200304008.
Jolly C, Sattentau QJ: Human immunodeficiency virus type 1 assembly, budding, and cell-cell spread in T cells take place in tetraspanin-enriched plasma membrane domains. J Virol. 2007, 81: 7873-7884. 10.1128/JVI.01845-06.
Booth AM, Fang Y, Fallon JK, Yang JM, Hildreth JE, Gould SJ: Exosomes and HIV Gag bud from endosome-like domains of the T cell plasma membrane. J Cell Biol. 2006, 172: 923-935. 10.1083/jcb.200508014.
Ho SH, Martin F, Higginbottom A, Partridge LJ, Parthasarathy V, Moseley GW, Lopez P, Cheng-Mayer C, Monk PN: Recombinant extracellular domains of tetraspanin proteins are potent inhibitors of the infection of macrophages by human immunodeficiency virus type 1. J Virol. 2006, 80: 6487-6496. 10.1128/JVI.02539-05.
von Lindern JJ, Rojo D, Grovit-Ferbas K, Yeramian C, Deng C, Herbein G, Ferguson MR, Pappas TC, Decker JM, Singh A, et al: Potential role for CD63 in CCR5-mediated human immunodeficiency virus type 1 infection of macrophages. J Virol. 2003, 77: 3624-3633. 10.1128/JVI.77.6.3624-3633.2003.
Gordon-Alonso M, Yanez-Mo M, Barreiro O, Alvarez S, Munoz-Fernandez MA, Valenzuela-Fernandez A, Sanchez-Madrid F: Tetraspanins CD9 and CD81 modulate HIV-1-induced membrane fusion. J Immunol. 2006, 177: 5129-5137.
Yoshida T, Kawano Y, Sato K, Ando Y, Aoki J, Miura Y, Komano J, Tanaka Y, Koyanagi Y: A CD63 mutant inhibits T-cell tropic human immunodeficiency virus type 1 entry by disrupting CXCR4 trafficking to the plasma membrane. Traffic. 2008, 9: 540-558. 10.1111/j.1600-0854.2007.00700.x.
Tardif MR, Tremblay MJ: Tetraspanin CD81 provides a costimulatory signal resulting in increased human immunodeficiency virus type 1 gene expression in primary CD4+ T lymphocytes through NF-kappaB, NFAT, and AP-1 transduction pathways. J Virol. 2005, 79: 4316-4328. 10.1128/JVI.79.7.4316-4328.2005.
Chen H, Dziuba N, Friedrich B, von Lindern J, Murray JL, Rojo DR, Hodge TW, O'Brien WA, Ferguson MR: A critical role for CD63 in HIV replication and infection of macrophages and cell lines. Virology. 2008, 379: 191-196. 10.1016/j.virol.2008.06.029.
Grigorov B, Attuil-Audenis V, Perugi F, Nedelec M, Watson S, Pique C, Darlix JL, Conjeaud H, Muriaux D: A role for CD81 on the late steps of HIV-1 replication in a chronically infected T cell line. Retrovirology. 2009, 6: 28-10.1186/1742-4690-6-28.
Ruiz-Mateos E, Pelchen-Matthews A, Deneka M, Marsh M: CD63 is not required for production of infectious human immunodeficiency virus type 1 in human macrophages. J Virol. 2008, 82: 4751-4761. 10.1128/JVI.02320-07.
Khurana S, Krementsov DN, de Parseval A, Elder JH, Foti M, Thali M: Human immunodeficiency virus type 1 and influenza virus exit via different membrane microdomains. J Virol. 2007, 81: 12630-12640. 10.1128/JVI.01255-07.
Singethan K, Muller N, Schubert S, Luttge D, Krementsov DN, Khurana SR, Krohne G, Schneider-Schaulies S, Thali M, Schneider-Schaulies J: CD9 clustering and formation of microvilli zippers between contacting cells regulates virus-induced cell fusion. Traffic. 2008, 9: 924-935. 10.1111/j.1600-0854.2008.00737.x.
Sato K, Aoki J, Misawa N, Daikoku E, Sano K, Tanaka Y, Koyanagi Y: Modulation of human immunodeficiency virus type 1 infectivity through incorporation of tetraspanin proteins. J Virol. 2008, 82: 1021-1033. 10.1128/JVI.01044-07.
Hubner W, Chen P, Del Portillo A, Liu Y, Gordon RE, Chen BK: Sequence of human immunodeficiency virus type 1 (HIV-1) Gag localization and oligomerization monitored with live confocal imaging of a replication-competent, fluorescently tagged HIV-1. J Virol. 2007, 81: 12596-12607. 10.1128/JVI.01088-07.
Qin XF, An DS, Chen IS, Baltimore D: Inhibiting HIV-1 infection in human T cells by lentiviral-mediated delivery of small interfering RNA against CCR5. Proc Natl Acad Sci USA. 2003, 100: 183-188. 10.1073/pnas.232688199.
Wehrly K, Chesebro B: p24 antigen capture assay for quantification of human immunodeficiency virus using readily available inexpensive reagents. Methods. 1997, 12: 288-293. 10.1006/meth.1997.0481.
Weng J, Krementsov DN, Khurana S, Roy NH, Thali M: Formation of syncytia is repressed by tetraspanins in HIV-1 producing cells. J Virol. 2009, 83: 7467-7474. 10.1128/JVI.00163-09.
Wei X, Decker JM, Liu H, Zhang Z, Arani RB, Kilby JM, Saag MS, Wu X, Shaw GM, Kappes JC: Emergence of resistant human immunodeficiency virus type 1 in patients receiving fusion inhibitor (T-20) monotherapy. Antimicrob Agents Chemother. 2002, 46: 1896-1905. 10.1128/AAC.46.6.1896-1905.2002.
Lekishvili T, Fromm E, Mujoomdar M, Berditchevski F: The tumour-associated antigen L6 (L6-Ag) is recruited to the tetraspanin-enriched microdomains: implication for tumour cell motility. J Cell Sci. 2008, 121: 685-694. 10.1242/jcs.020347.
Gervaix A, West D, Leoni LM, Richman DD, Wong-Staal F, Corbeil J: A new reporter cell line to monitor HIV infection and drug susceptibility in vitro. Proc Natl Acad Sci USA. 1997, 94: 4653-4658. 10.1073/pnas.94.9.4653.
Spenlehauer C, Gordon CA, Trkola A, Moore JP: A luciferase-reporter gene-expressing T-cell line facilitates neutralization and drug-sensitivity assays that use either R5 or X4 strains of human immunodeficiency virus type 1. Virology. 2001, 280: 292-300. 10.1006/viro.2000.0780.
Arora VK, Fredericksen BL, Garcia JV: Nef: agent of cell subversion. Microbes Infect. 2002, 4: 189-199. 10.1016/S1286-4579(01)01527-1.
Pique C, Lagaudriere-Gesbert C, Delamarre L, Rosenberg AR, Conjeaud H, Dokhelar MC: Interaction of CD82 tetraspanin proteins with HTLV-1 envelope glycoproteins inhibits cell-to-cell fusion and virus transmission. Virology. 2000, 276: 455-465. 10.1006/viro.2000.0538.
Mazurov D, Heidecker G, Derse D: HTLV-1 Gag protein associates with CD82 tetraspanin microdomains at the plasma membrane. Virology. 2006, 346: 194-204. 10.1016/j.virol.2005.10.033.
Mazurov D, Heidecker G, Derse D: The inner loop of tetraspanins CD82 and CD81 mediates interactions with human T cell lymphotrophic virus type 1 Gag protein. J Biol Chem. 2007, 282: 3896-3903. 10.1074/jbc.M607322200.
Pizzato M, Helander A, Popova E, Calistri A, Zamborlini A, Palu G, Gottlinger HG: Dynamin 2 is required for the enhancement of HIV-1 infectivity by Nef. Proc Natl Acad Sci USA. 2007, 104: 6812-6817. 10.1073/pnas.0607622104.
We thank Yoshio Koyanagi, Kei Sato, Wes Sundquist, Hyo-Young Chung, Clarisse Berlioz-Torrent, Benjamin Chen, and John Guatelli for gifts of plasmids and/or helpful discussions and advice, and the NIH AIDS Reagent program for providing many high quality research reagents. We also thank the reviewers of an NIH grant proposal for their thoughtful critiques, which helped to clarify some of our working hypotheses. This work was supported by UVM COM Bridge Support and NIH grants RO1 AI47727 and R56 AI047727, as well as a Vermont Genetics Network Graduate Fellowship and T32 AI055402 Training Grant to D.K.
The authors declare that they have no competing interests.
DNK, JW, and MT designed experiments and analyzed data. DNK performed most of the experiments. ML performed the experiments shown in Figure 6C. NHR. designed and cloned the shRNA constructs, and optimized the shRNA-mediated knockdowns. DNK and MT wrote the manuscript.
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Krementsov, D.N., Weng, J., Lambelé, M. et al. Tetraspanins regulate cell-to-cell transmission of HIV-1. Retrovirology 6, 64 (2009) doi:10.1186/1742-4690-6-64
- HeLa Cell
- Jurkat Cell
- Syncytium Formation
- CD63 Overexpression
- Virological Synapse