- Short report
- Open Access
Downregulation of CD94/NKG2A inhibitory receptors on CD8+T cells in HIV infection is more pronounced in subjects with detected viral load than in their aviraemic counterparts
© Zeddou et al; licensee BioMed Central Ltd. 2007
- Received: 02 April 2007
- Accepted: 10 October 2007
- Published: 10 October 2007
The CD94/NKG2A heterodimer is a natural killer receptor (NKR), which inhibits cell-mediated cytotoxicity upon interaction with MHC class I gene products. It is expressed by NK cells and by a small fraction of activated CD8+ T lymphocytes. Abnormal upregulation of the CD94/NKG2A inhibitory NKR on cytotoxic T cells (CTLs) could be responsible for a failure of immunosurveillance in cancer or HIV infection. In this study, CD94/NKG2A receptor expression on CD8+ T lymphocytes and NK cells was assessed in 46 HIV-1-infected patients (24 viraemic, 22 aviraemic) and 10 healthy volunteers. The percentage of CD8+ T lymphocytes expressing the CD94/NKG2A inhibitory heterodimer was very significantly decreased in HIV-1-infected patients in comparison with non-infected controls. Within the HIV infected patients, the proportion of CD8+ T lymphocytes and NK cells expressing CD94/NKG2A was higher in subjects with undetectable viral loads in comparison with their viraemic counterparts. No significant difference was detected in the proportion of CD8+ T lymphocytes expressing the activatory CD94/NKG2C heterodimer between the HIV-1 infected patients and the healthy donors, nor between the vireamic and avireamic HIV-1 infected patients. In conclusion, chronic stimulation with HIV antigens in viraemic patients leads to a decreased rather than increased CD94/NKG2A expression on CD8+ T lymphocytes and NK cells.
- Inhibitory Receptor
- Natural Killer Receptor
- Chronic Antigenic Stimulation
- Polyoma Virus Infection
- Inhibitory Natural Killer Receptor
The CD94/NKG2 heterodimer is a C-type lectin receptor, formed by the covalent association of CD94, a protein with a short non-signaling intracytoplasmic tail , and one of the NKG2 molecules. To generate a functional receptor, CD94 is disulfide linked with a member of the NKG2 family, namely NKG2A, -B, -C or -E [2, 3]. In humans, CD94/NKG2A interacts with complexes of non-classical HLA-E molecules [4, 5]. The intracellular domain of NKG2A contains immunoreceptor tyrosine-based inhibition motifs (ITIMs), responsible for transducing inhibitory signals . The other NKG2 members lack ITIMs and are linked to transmembrane proteins, such as DAP10 and DAP12 which contain immunoreceptor tyrosine-based activating motifs and transduce activating signals . CD94/NKG2A is normally expressed on most NK cells and on a small fraction of CD8+ T lymphocytes. The proportion of NK cells bearing the CD94/NKG2A inhibitory receptor decreases in advanced HIV infection , in contrast with other inhibitory receptors of the KIR family which are upregulated. It is presently unknown if HIV infection has similar effects on the expression of the CD94/NKG2A inhibitory receptor by CD8+ T cells. A few studies have shown that CD94 expression by CD8+ T cells is increased during HIV infection [9–11] and have led to postulate that increased expression of the CD94/NKG2A inhibitory receptors is one of the mechanisms rendering HIV-specific CD8+ T lymphocytes unable to control HIV-1 infection . Nevertheless, the simultaneous expression of both subunits of the inhibitory receptor on CD8+ T cells has hardly been studied in HIV infection. Costa et al. using two-color FACS analysis to study CD3+ NKG2A+ T cells, showed no difference between uninfected controls, long term non progressors or aviraemic subjects under HAART. A slight increase was noted in subjects with active viral replication , in contradiction with the downregulation previously observed on NK cells from infected subjects.
In HIV infected patients, there was a weak but significant correlation between the proportion of CD8+ T lymphocytes and NK cells expressing the CD94/NKG2A heterodimer (r2 = 0,09184; p = 0.0406) and the proportion of NK cells expressing the inhibitory receptor tended to be lower in viraemic patients than in subjects with less than 50 copies/ml. (mean ± SEM, 43.11 ± 5.67% vs. 56.05 ± 4.67%; p = 0.019). There was no correlation of the expression of the inhibitory receptor with absolute or relative CD4 counts (data not shown).
In summary, we observed a downregulation of CD94/NKG2A on CD8+ T cells in HIV infection, in accordance with what was previously described for NK cells. The mechanisms linking viral replication with downregulation of the inhibitory CD94/NKG2A receptor remains obscure. Upregulation of CD94/NKG2A has previously been observed in various animal models of viral and bacterial infections  and in chronic antigenic stimulation . Loss of CD94/NKG2A might correspond to the terminal differentiation which occurs in a large fraction of CD8+ T cells during HIV infection. Indeed, recent observations made in an experimental model of persistent polyoma virus infections suggest that CD94/NKG2A CD8+ T lymphocytes might constitute a less differentiated subset of CD8+ T cells and maintain a higher proliferative potential and capacity to secrete IL-2 . Whatever is the mechanism involved, the loss of CD94/NKG2A in HIV infection could also contribute to the polyclonal activation which characterizes HIV infection.
This work was supported by the Fond National de la Recherche Scientifique (FNRS), the Centre anticancéreux près l'Université de Liège (CAC) and a grant from the Walloon Region.
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