- Open Access
Isolated receptor binding domains of HTLV-1 and HTLV-2 envelopes bind Glut-1 on activated CD4+ and CD8+ T cells
- Sandrina Kinet†1, 2, 3,
- Louise Swainson†1, 2, 3,
- Madakasira Lavanya1, 2, 3,
- Cedric Mongellaz1, 2, 3,
- Amélie Montel-Hagen1, 2, 3,
- Marco Craveiro1, 2, 3,
- Nicolas Manel1, 2, 3, 4,
- Jean-Luc Battini1, 2, 3,
- Marc Sitbon and
- Naomi Taylor1, 2, 3Email author
© Kinet et al; licensee BioMed Central Ltd. 2007
- Received: 25 March 2007
- Accepted: 15 May 2007
- Published: 15 May 2007
We previously identified the glucose transporter Glut-1, a member of the multimembrane-spanning facilitative nutrient transporter family, as a receptor for both HTLV-1 and HTLV-2. However, a recent report concluded that Glut-1 cannot serve as a receptor for HTLV-1 on CD4 T cells: This was based mainly on their inability to detect Glut-1 on this lymphocyte subset using the commercial antibody mAb1418. It was therefore of significant interest to thoroughly assess Glut-1 expression on CD4 and CD8 T cells, and its association with HTLV-1 and -2 envelope binding.
As previously reported, ectopic expression of Glut-1 but not Glut-3 resulted in significantly augmented binding of tagged proteins harboring the receptor binding domains of either HTLV-1 or HTLV-2 envelope glycoproteins (H1RBD or H2RBD). Using antibodies raised against the carboxy-terminal peptide of Glut-1, we found that Glut-1 expression was significantly increased in both CD4 and CD8 cells following TCR stimulation. Corresponding increases in the binding of H1RBD as well as H2RBD, not detected on quiescent T cells, were observed following TCR engagement. Furthermore, increased Glut-1 expression was accompanied by a massive augmentation in glucose uptake in TCR-stimulated CD4 and CD8 lymphocytes. Finally, we determined that the apparent contradictory results obtained by Takenouchi et al were due to their monitoring of Glut-1 with a mAb that does not bind cells expressing endogenous Glut-1, including human erythrocytes that harbor 300,000 copies per cell.
Transfection of Glut-1 directly correlates with the capacities of HTLV-1 and HTLV-2 envelope-derived ligands to bind cells. Moreover, Glut-1 is induced by TCR engagement, resulting in massive increases in glucose uptake and binding of HTLV-1 and -2 envelopes to both CD4 and CD8 T lymphocytes. Therefore, Glut-1 is a primary binding receptor for HTLV-1 and HTLV-2 envelopes on activated CD4 as well as CD8 lymphocytes.
- Glucose Uptake
- 293T Cell
- Jurkat Cell
- Receptor Binding Domain
- mAb1418 Staining
We identified the ubiquitous glucose transporter Glut-1 as a receptor for deltaretrovirus HTLV-1 and HTLV-2 envelopes (Env), mediating viral binding and entry . We further identified Glut-1 extracellular loop 6 (ECL6) as the primary binding site for both HTLV-1 and 2 receptor binding domains (RBD) . The identity of the HTLV Env receptor remained elusive for approximately two decades and the search was hindered by the fact that HTLV entry can take place in all established vertebrate cell lines and generally produces a rampant syncytial effect. This long search has been the source of numerous speculations as to the nature of the receptor, including the possibility that a dedicated cellular receptor may not be required for HTLV infection or that many different receptors can be used by HTLV . The elucidation of the modular organization of the HTLV Env is based on that of a gammaretrovirus Env : The identification and generation of tagged fusion proteins that comprise the RBD of the HTLV-1 and -2 Env, in the absence of the carboxy terminal domain [4, 5], were essential to our finding that Glut-1 is a receptor for HTLV Env.
Biochemical studies assessing cell surface Glut-1 have been hampered by the lack of antibodies recognizing extracellular determinants of this transporter. This difficulty was in large part due to the high degree of homology between the Glut-1 extracellular domains of diverse mammalian species and indeed, studies aimed at generating antibodies to all domains of Glut-1 concluded that the extracellular loops are non-antigenic [6, 7]. Notably, this high conservation of Glut-1 is likely responsible for the ability of HTLV-1 to infect all tested vertebrate cell lines. Recently though, a monoclonal antibody promoted as recognizing an extracellular domain of Glut-1, thereby allowing detection of surface Glut-1, has been made commercially available (RnD systems, mAb1418). Using this antibody, Takenouchi et al. did not detect binding on quiescent or activated CD4 T lymphocytes, a major reservoir of HTLV in vivo, leading these authors to question the role of Glut-1 as primary binding receptor for HTLV .
Numerous biochemical and cell biology experiments from our laboratory and others strongly support the role of Glut-1 as a receptor for both HTLV-1 and HTLV-2 [1, 2, 5, 9–12]. It was therefore of significant interest to reassess Glut-1 expression on quiescent and activated CD4 as well as CD8 T cells as well as to analyze the relevance of the mAb1418 with regards to detection of Glut-1 expression.
Binding of Glut-1 antibodies and HRBD-derived ligands to 293T cells transfected with Glut-1 and Glut-3 glucose transporters
As mentioned above, even though antibodies directed against Glut-1 extracellular epitopes are problematic to obtain, polyclonal and monoclonal antibodies directed against the intracellular carboxy terminus tail (C-term) have been reported by several teams [6, 15–18]. Indeed, using this type of intracellular polyclonal antibody directed against the C-term domain of Glut-1, we detected the expected baseline expression of endogenous Glut-1 in 293T cells. Moreover, an increase in Glut-1 staining was revealed following Glut-1 transfection (Fig. 1A). As 293T cells are known to express Glut-1 (see below), these data indicate that either the level of endogenous Glut-1 was below the level of detection necessary for mAb1418 staining or alternatively, mAb1418 does not recognize endogenous Glut-1 on these cells.
Notably, the ability of HTLV-1 and HTLV-2 derived RBDs to bind to parental and transfected 293T cells correlated with the data obtained using the C-term Glut-1 antibody. As we expected from previous studies, extracellular binding of rFc-fusion proteins encoding either H1RBD or H2RBD was significantly augmented in cells transfected with Glut-1 but not Glut-3 (Fig. 1B). Additionally, an H2RBD-EGFP fusion protein, that was concentrated to promote maximal binding, showed a similar profile (Fig. 1B).
These results contrast with those reported by Takenouchi et al., who did not detect increased binding to Glut-1-transfected COS-7 cells when the entire HTLV-1 SU was used as a ligand . Significantly though, these latter binding studies were performed on ice, and in our hands, neither H1RBD nor H2RBD bind to Glut-1-expressing cells under those conditions (data not shown). Rather, in absence of fixation, binding of the HTLV RBDs requires incubation at physiological conditions, namely 37°C.
In contrast to a lack of extracellular or intracellular staining by mAb1418, both H1RBD and H2RBD interacted with extracellular Glut-1 resulting in significantly higher mean fluorescence intensity bindings to human erythrocytes as compared to either the 293T or Jurkat cell lines (Fig. 2A). Moreover, the level of H1RBD and H2RBD binding, but not that of mAb1418 staining, correlated directly with the total cellular levels of Glut-1 as detected by immunoblot (Fig. 2B). It should be noted that changes in the mobility of Glut-1 and the appearance of a smear in erythrocytes are well known and have been shown to be due in part to post-translational N-glycosylation modifications . Indeed, treatment of cells with tunicamycin, a drug that inhibits N-linked glycosylation, or trimming of N-linked glycans with N-glycosidase F increases the electrophoretic mobility of Glut-1 .
These data lead to the question as to how the mAb1418 antibody was selected as being "specific" to Glut-1. According to the manufacturer's indications, this antibody was generated by immunization of mice with a murine cell line transfected with human Glut-1. The criterion for hybridoma selection was based on the ability to bind the transfected cell line and not the parental cell line. Thus, the capacity of this antibody to recognize endogenous Glut-1 was not specifically the basis for selection. The ensemble of the data presented here strongly indicates that mAb1418 does not detect endogenous Glut-1 but rather interacts with a cell surface protein that is associated with Glut-1 overexpression in transformed cell lines.
TCR-induced expression of Glut-1 on both CD4+ and CD8+ T cells results in H1RBD/H2RBDbinding and glucose uptake
These data are in complete contradiction with the long-standing notions that; 1) quiescent T cells demonstrate very low glucose transport and 2) the energy demands of an activated T cell are met by an increase in glucose transport as well as metabolism [20–23]. The main functional glucose transporter isoform on CD4 as well as CD8 T lymphocytes has been reported to be Glut-1. In fact, a large body of work by several laboratories has established that Glut-1 is expressed at low levels on quiescent T cells but is induced upon T cell receptor activation as well as cytokine stimulation [9, 16, 24–29]. Moreover, in the absence of stimulation or transformation, surface Glut-1 is not detected on T cells of naïve, memory or effector phenotypes [9, 16].
To further assess Glut-1 expression on quiescent and activated T cell subsets, we used the intracellular C-term anti-Glut-1 polyclonal antibody raised against a peptide encoding the 13 C-terminal amino acids. As expected from the literature cited above, Glut-1 expression was significantly increased in both CD4 and CD8 T cells following TCR stimulation (Fig. 3A), a time at which these cells had become blast-like and expressed activation markers such as CD25 and CD69 (data not shown). In parallel, binding of H1RBD as well as H2RBD, not detected on quiescent T cells, was clearly augmented following TCR stimulation. These data are in contradiction with that of Jones and colleagues who do not detect binding of the HTLV-2 SU to activated CD4 T cells , but several technical differences in the receptor binding assays are likely to explain this discrepancy. Indeed, we use isolated RBDs, in the absence of the adjacent Env SU domains that may alter primary receptor binding. Also, our RBDs are produced as soluble secreted proteins, whereas Jones et al. use entire SU immunoadhesins prepared from sonicated whole cell extracts which are likely to contain heterogeneous post-translationally modified proteins. Furthermore, we performed RBD binding assays at 37°C as neither binding of H1RBD nor H2RBD is detected at 4°C (data not shown). Notably, this is also the case for many RBDs from gammaretroviruses, including amphotropic, xenotropic, and FeLV-C (our unpublished observations). Using the SU immunoadhesins, Jones et al. perform their HTLV envelope binding assays at 4°C on fixed cells.
Staining with the anti-Glut-1 polyclonal antibody revealed low binding to permeabilized quiescent CD4 and CD8 T cells while no detectable surface staining was observed on either quiescent cell types when using HRBD ligands. This difference may be due to cell permeabilization prior to antibody staining, resulting in the recognition of a small intracellular pool of Glut-1 that is not present at the cell surface. In support of the latter hypothesis, Baldwin and colleagues have elegantly shown that cytokines and other growth signals induce the translocation of cytoplasmic Glut-1 to the cell surface in several different cell models [31–33]. In this context, it is interesting to note that stable expression of GFP-tagged Glut-1 results in its accumulation in intravesicular pools, whereas Glut-3 appears almost exclusively expressed at the cell surface (data not shown).
The expected primary physiological consequence of cell surface Glut-1 expression is glucose transport. It was therefore of interest to assess glucose uptake on quiescent and activated CD4+ and CD8+ lymphocyte subsets. Glucose uptake, as measured by the ability of cells to uptake nonhydrolyzable 2-deoxy-D [1-3H]glucose, was at the limits of detection in both quiescent CD4 and CD8 T cells and increased by >20-fold in both subsets following stimulation (Fig. 3B). Notably, the increase in glucose uptake was equivalent in the CD4 and CD8 populations, demonstrating yet again that only activated lymphocytes express high surface levels of a glucose transporter. These results are in total agreement with other studies assessing glucose transporter expression in T lymphocytes [10, 16, 24, 25]. Although it is not clear what cell surface protein is recognized by mAb1418 on quiescent CD8 T cells, these results indicate that the cognate antigen of mAb1418 is not a member of the glucose transporter family.
Induction of surface Glut-1, both upon exogenous transfection and stimulation of the endogenous protein, directly correlates with the capacities of HTLV-1 and HTLV-2 envelope-derived ligands to bind cells. We have previously reported a physical interaction between HTLV-1 and HTLV-2 RBDs and Glut-1 and mapped the primary binding site to ECL6 . Moreover, down-modulation of endogenous Glut-1 by siRNAs resulted in a significant reduction in HTLV-1 and HTLV-2 Env binding as well as Env-mediated infection . In theses systems, as well as in recently published studies by others, Glut-1 was found to serve as cell surface receptor for both HTLV-1 and -2 infection [1, 2, 10–12].
Retroviral entry has been extensively shown to depend on several steps. These include initial receptor binding ensured by RBD-harboring amino terminal domains of the SU, followed by subsequent conformational changes and Env-receptor complex remodeling that leads to the unmasking of the TM fusion peptide and membrane fusion, either at the cell surface or after endocytosis and acidification of endocytic vesicles. Using Glut-1 and Glut-3 chimeric molecules, we have previously shown that while the Glut-1 ECL6 was sufficient to confer HTLV Env binding to Glut-3, ECL1 and 5 were required for HTLV-Env-mediated infection . Therefore, successful post-binding events involving HTLV Env regions located outside of HRBD in conjunction with Glut-1 ECL1 and 5 are likely to depend on other co-factors. In this regard, it is interesting to note that neuropilin-1 and HSC70 have been shown to interact with the SU region located immediately downstream of HRBD [12, 36]. Heparan sulfates, which modulate the attachment of numerous viruses [3, 37], are also likely to influence the surface environment conditioning HTLV entry following Env-Glut-1 interactions .
Therefore, it will be essential to more precisely define the cell surface environment in which Glut-1 is expressed in distinct cell types in order to determine the parameters that condition HTLV envelope binding and subsequent infection. Identification of cellular factors that modulate the localization of Glut-1 as well as its cell surface membrane microenvironment are likely to shed new insights into our global understanding of HTLV in vivo tropism and associated physiopathologies.
293T cells were transfected with the Glut-1 and Glut-3 expression vectors using the calcium phosphate method. Human Glut-1 and Glut-3 cDNAs were amplified by PCR from the pLib HeLa cDNA library (Clontech), and inserted into pCHIX, a modified version of the pCSI vector that includes a C-terminal factor Xa cleavage site, and the hemagglutinin (HA) and histidine tags . After an overnight transfection, cells were washed in phosphate-buffered saline (PBS), fresh medium was added and cells were cultured for an additional 24 h.
T cell isolation and culture
To avoid artifactual stimulation, CD4+ and CD8+ T cells were each purified by negative selection using tetrameric complexes in which one antibody recognizes a surface antigen on B cells, monocytes, NK cells or CD8+ vs CD4+ cells, respectively, and the other recognizes glycophorin A on the surface of red blood cells (RosetteSep, StemSep Technologies, Vancouver, Canada). The non-purified cells were then pelleted upon ficoll-hypaque separation. The purity of the selected cells was monitored after each isolation on a FACSCalibur (BD Pharmingen, San Jose, CA, USA) following staining with FITC-conjugated αCD3 and phycoerythrin-conjugated αCD4 mAbs or -αCD8 mAbs (Beckman Coulter, Marseille, France). The purity of the selected cells was > 90%.
Lymphocytes were cultured in RPMI 1640 medium supplemented with 10% fetal calf serum (FCS, BioWest, France), penicillin and streptomycin. Cells were stimulated with immobilized αCD3 (UCHT-1) and αCD28mAbs (1 μg/ml) for two to four days.
Generation of HTLV-1 and HTLV-2 RBD fusion proteins
The H1215SU and H2178SU subdomains, corresponding to the HTLV-1 and -2 SU amino terminus were generated by PCR and subcloned into the pCSI expression vector as fusion proteins harboring a carboxy terminal rFc tag , and are herein referred to as H1RBD and H2RBD, respectively. Additionally, the amino terminal 178 amino acids of the HTLV-2 RBD was fused to the EGFP coding sequence (from pEGFP-N3, Clontech) lacking the ATG initiation codon by PCR amplification in the pCSI expression vector and is herein referred to as H2RBD-EGFP. 293T cells were transfected with the indicated vector using a calcium-phosphate-Hepes buffered saline (HBS) transfection protocol. Transfection medium was replaced with fresh culture medium twenty hours post-transfection. Forty-eight hours post-transfection cell culture medium (supernatant) was recovered and filtered through a 0.45 μm pore-size membrane to remove cell debris. H2RBD-EGFP supernatants were concentrated 50–100-fold by filtration at 4°C on an iCON Concentrator 20 K (Pierce, Rockford, IL, USA). Supernatants were stored at -20°C until further use.
Staining with the mAb1418 (RnD Systems) was performed by incubating 1 × 105 cells for 20 min on ice at 1:25 to 1:100 antibody dilutions followed by staining with a FITC-conjugated goat anti mouse IgG (Sigma) at a 1:100 dilution. Background fluorescence was measured following staining with the secondary antibody alone. Staining with the anti-carboxy terminal Glut-1 polyclonal antibody (generously provided by A. Carruthers) was performed following intracellular staining by fixation (Cytofix Cytoperm solution, BD Pharmingen) and permeabilization (PhosFlow Perm III, BD Pharmingen). The secondary antibody was a FITC-conjugated goat anti-rabbit IgG (Sigma). For HRBD stainings, 1–5 × 105 cells were incubated at 37°C for 30 min with 100–500 μls of either undiluted HRBD-rFc supernatants or a 1:25–1:50 dilution of the concentrated H2RBD-EGFP ligand. Cells were then washed with PBA (2% FCS and 0.01% sodium azide), and for H1RBD and H2RBD stainings, cells were incubated with a FITC-conjugated sheep anti-rabbit Fc antibody (1:500 dilution; Sigma) for 20 min on ice. Cells were analysed on a FACSCalibur flow cytometer (Becton Dickinson). Data analyses were performed using CellQuest Pro (Becton Dickinson) or FlowJo (TreeStar) software.
Non-boiled lysates from 293T, Jurkat, and human erythrocytes were electrophoresed in SDS-10% acrylamide gels, transferred and probed with the anticarboxy terminal Glut-1 antibody (1:10,000) followed by a peroxidase-conjugated anti-rabbit immunoglobulin antiserum. Proteins were visualized using the ECLplus kit (Amersham).
5 × 105 CD4+ or CD8+ T cells were incubated at 37°C in serum-free RPMI for 30 min, then washed and incubated for an additional 30 min in serum/glucose-free RPMI. Uptake was initiated by adding labeled 2-deoxy-D [1-3H]glucose (Amersham Biosciences; 2 μCi/ml) to an unlabelled deoxy-glucose concentration of 0.1 mM and incubating cells for 45 min at 37°C. Alternatively, glucose uptake was performed in a 50 μl volume in the presence of 5 μM 2-deoxy-D [1-3H]glucose (2 μCi) for 10 min at RT. Cells were then washed in cold serum/glucose-free RPMI, and solubilized in 0.1% SDS. Radioactivity was measured by liquid scintillation and statistical analyses were performed using Student's t test.
We are grateful to all members of our laboratories for their critical input and advice. We are indebted to A. Carruthers and M. Mueckler for their advice on Glut-1 biology and for generously providing us with carboxy terminal anti-Glut-1 antibodies. L.S. was supported by successive grants from the ANRS, Sidaction and the European Community (contract LSHC-CT-2005-018914 "ATTACK"). A.M.-H. is supported by a grant from the French Ministery of Education and L.M. by the AFM. C.M. was supported by SIDACTION and the Fondation de France and is now employed by the CNRS. S.K. is supported by the CNRS and J-L.B., M.S. and N.T all supported by INSERM. This work was funded by grants from the European Community (contract LSHC-CT-2005-018914 "ATTACK"), the ANRS, Sidaction, Fondation de France and the AFM.
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