Figure 2
Endogenous Glut-1 expression in diverse cell types is not reflected by mAb1418 reactivity but correlates with binding of the HTLV-1 and HTLV-2 Env RBDs. (A) 293T, Jurkat and primary human erythrocytes were stained with mAb1418 and control binding with the secondary FITC-conjugated antibody is shown in all histograms (filled). Intracellular Glut-1 levels in permeabilized 293T and Jurkat cells were monitored with mAb1418 as well as the C-term polyclonal Glut-1 antibody. Expression of the HTLV-1 and HTLV-2 receptor was monitored by incubation of cells for 30 min at 37°C with the rFc-tagged H1RBD and H2RBD fusion proteins as well as the H2RBD-EGFP fusion protein. Binding is shown in solid line histograms whereas control immunofluorescence is shown in filled histograms. (B) Total Glut-1 protein levels in cell extracts from 293T, Jurkat and human erythrocytes were monitored by immunoblotting with an anti-C-term Glut-1 antibody.