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Frequent genetic defects in long-term survivors for more than 26 years in the absence of antiretroviral therapy in Korea: its association with ginseng treatment

  • 1,
  • 1 and
  • 1
Retrovirology201310 (Suppl 1) :P14

https://doi.org/10.1186/1742-4690-10-S1-P14

  • Published:

Keywords

  • Peripheral Blood Mononuclear Cell
  • Medicinal Plant
  • Genetic Defect
  • Stop Codon
  • Significant Period

Background

The association between long-term nonprogressors (LTNPs) and genetic defects in HIV-1 is not clear because of paucity of genetic defects in LTNPs. To date, there is only a report on gross deletion in 2 genes of HIV-1. Ginseng has been used as the premier medicinal plant for a thousand years [1]. Korean red ginseng (KRG) has been applied to HIV-1 infected patients since November 1991 [2]. We have found that most HIV-1 infected patients treated with KRG for a significant period slowly progressed to AIDS and revealed a high frequency of genetic defects.

Materials and methods

A total of 250 individuals were diagnosed with HIV-1 infection in Korea before 1993. Among the 250 individuals, we selected all who remain healthy for >18 years in the absence of antiretroviral therapy. They were four individuals (26, 25, 21, and 18 years from diagnosis). One patient who was diagnosed with HIV-1 in 1992 took a little KRG – less than 1,000 g. To investigate whether LTNPs are associated with genetic defects, we amplified near full-length HIV-1 sequences in 10 overlapping fragments (0.8 to 1.4 kb) using nested PCR with peripheral blood mononuclear cells. We compared the data with that in 4 control patients. Amount of KRG supplied was significantly higher in LTNPs (21,767-8,866 g) than 1,250-1,304 g in controls (P < 0.01).

Results

We obtained 1,339 and 386 PCR amplicons over 20 years in LTNPs and over 10 years in controls, respectively. All LTNPs revealed at least one genetic defect in 4 genes, whereas controls revealed it in both 5’ LTR/gag and nef genes. Overall genetic defects were significantly higher in LTNPs (11.4%) than 4.9% in controls (P < 0.001). Among the 4 LTNPs, one patient who had taken the least amount of KRG revealed significantly higher proportion of premature stop codons (5.6%) than 1.6% in 3 LTNPs (P < 0.01). There was a significant difference in the frequency of genetic defects between on KRG (10.9%) and baseline prior to KRG intake (4.3%) (P < 0.01). There was a significant correlation between amount of KRG supplied and proportion of genetic defects (r=0.80, P < 0.05). At single gene level, only the nef gene showed a significantly higher frequency of genetic defects in LTNPs (11.5%) than 3.6% in controls (P < 0.01).

Conclusion

These data show the possibility that frequent genetic defects that might be caused by KRG treatment are associated with long-term slow progression.

Authors’ Affiliations

(1)
Microbiology, University of Ulsan College of Medicine, SEOUL, Republic of Korea

References

  1. Attele AS, Wu JA, Yuan CS: Ginseng pharmacology: multiple constituents and multiple actions. Biochem Pharmacol. 1999, 58: 1685-93. 10.1016/S0006-2952(99)00212-9.View ArticlePubMedGoogle Scholar
  2. Sung H, Kang SM, Lee MS, Kim TG, Cho YK: Korean red ginseng slows depletion of CD4T cells in HIV-1-infected patients. Clin Diagn Lab Immunol. 2005, 12: 497-501.PubMed CentralPubMedGoogle Scholar

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