- Open Access
Reticuloendotheliosis virus and avian leukosis virus subgroup J synergistically increase the accumulation of exosomal miRNAs
© The Author(s) 2018
- Received: 14 March 2018
- Accepted: 21 June 2018
- Published: 3 July 2018
Co-infection with avian leukosis virus subgroup J and reticuloendotheliosis virus induces synergistic pathogenic effects and increases mortality. However, the role of exosomal miRNAs in the molecular mechanism of the synergistic infection of the two viruses remains unknown.
In this study, exosomal RNAs from CEF cells infected with ALV-J, REV or both at the optimal synergistic infection time were analysed by Illumina RNA deep sequencing. A total of 54 (23 upregulated and 31 downregulated) and 16 (7 upregulated and 9 downregulated) miRNAs were identified by comparing co-infection with two viruses, single-infected ALV-J and REV, respectively. Moreover, five key miRNAs, including miR-184-3p, miR-146a-3p, miR-146a-5p, miR-3538 and miR-155, were validated in both exosomes and CEF cells by qRT-PCR. GO annotation and KEGG pathway analysis of the miRNA target genes showed that the five differentially expressed miRNAs participated in virus-vector interaction, oxidative phosphorylation, energy metabolism and cell growth.
We demonstrated that REV and ALV-J synergistically increased the accumulation of exosomal miRNAs, which sheds light on the synergistic molecular mechanism of ALV-J and REV.
- Reticuloendotheliosis virus
- Avian leukosis virus subgroup J
- Exosomal miRNAs
- Synergistic infection
Viral synergism occurs commonly in nature when co-infection of two or more unrelated viruses invades the same host. Both reticuloendotheliosis virus (REV) and avian leukosis virus subgroup J (ALV-J), as two oncogenic retroviruses, consist of a set of retroviral genes, env, pol, gag and LTR, and mainly induce reticuloendotheliosis and myelocytomas, respectively [1, 2]. Due to similar transmission routes, co-infection with ALV-J and REV can readily occur [3, 4] and spreads very rapidly [5–7]. Co-infection of ALV-J and REV induces more serious pathogenic effects, such as immunosuppression, growth retardation, accelerated neoplasia progression, secondary infection in chickens [3, 5], and increased mortality. Exosomes, intraluminal vesicles ranging approximately 30-100 nm in diameter secreted by live cells, have emerged as important molecules for intercellular communication that are involved in both normal and pathophysiological conditions, such as lactation, immune response and neuronal function, and in the development and progression of diseases, such as liver disease, neurodegenerative diseases and cancer [8–17]. Exosomes contain a wide variety of proteins, lipids, RNAs, non-transcribed RNAs, microRNAs and small RNAs to induce a diverse range of functions from intercellular communication to tumour proliferation [14, 18]. As useful biomarkers, exosomes are also helpful for exploring the synergistic mechanisms of co-infection with ALV-J and REV.
MicroRNAs (miRNAs) constitute a large family of small noncoding RNAs functioning as major regulators of gene expression in cancer development [19–21]. The mature miRNA regulates spatio-temporal gene expression by binding to a seed region in the 3′ untranslated region (UTR) but may also bind to the 5′ UTR of target mRNA to enhance mRNA translation inhibition or degradation, resulting in decreased protein expression [22, 23]. Although miRNAs occupy negligible genomic space, their influence on a myriad of physiological processes, such as growth, differentiation, apoptosis, host–pathogen interactions and energy metabolism, is indubitable and relevant in tumour progression [24–30]. A growing number of studies have certified that miRNAs, including miR-122, miR-29b, miR-34a and miR-155, are key regulators of tumourigenesis, especially in viral synergistic infection [31–33]. However, the role of miRNA-mediated regulation of co-infection with ALV-J and REV remains unknown.
In the present study, to reveal the roles of miRNA profiles in the synergistic infection with ALV-J and REV, exosomal miRNAs were extracted from CEF infected with ALV-J, REV or both at the optimal synergistic infection time to analyse by Illumina RNA deep sequencing. The key miRNAs obtained from deep sequencing were validated in exosomes and CEFs by qRT-PCR. Furthermore, the affected miRNA–mRNA interactions and associated biological processes were defined by integrated target prediction analyses.
Synergistic infection of ALV-J and REV increases virus replication in CEF
Deep sequence analysis of exosomal miRNAs
Summary of deep sequencing data for small RNAs in ExoN, ExoJ, ExoR, or ExoRJ
Map to genome percent (%)
Identification of ALV-J and REV synergistic activated exosomal miRNAs in CEFs
Differentially expressed microRNAs between ExoRJ and ExoJ
Differentially expressed microRNAs between ExoRJ and ExoR
Validation of miRNA expression using qRT-PCR
Primers used to detect miRNA expression using qRT-PCR
In this study, an enhancement of viral transcription and protein expression was observed in ALV-J and REV co-infected CEF cells and reached a peak at 72 hpi. After Illumina small RNA deep sequencing of exosomes from CEFs co-infected with ALV-J and REV, a total of 54 (23 upregulated and 31 downregulated) and 16 (7 upregulated and 9 downregulated) miRNAs were identified by comparing the significantly differentially expressed miRNAs of ExoRJ with ExoJ and ExoR, respectively. Further, 5 miRNAs, including miR-184-3p, miR-146a-3p, miR-146a-5p, miR-3538 and miR-155, were verified by qRT-PCR and found to be consistent with the sequencing analysis. The analysis of the target prediction data demonstrated that these differentially expressed miRNAs participated in aspects of virus-vector interaction, oxidative phosphorylation, energy metabolism and cell growth in the process of co-infection with ALV-J and REV.
Useful as a biomarker, exosomes are overproduced by most proliferating cell types and contain a wide variety of microRNAs to induce a diverse range of functions, such as antigen presentation, cellular responses to environmental stresses, and propagation of pathogens [35–39]. Some miRNAs, such as let-7, miR-1, miR-15, miR-16, miR-151 and miR-375, which have roles in angiogenesis, haematopoiesis, exocytosis and tumourigenesis, have been reported in exosomes [40–44]. To further understand the relationship between exosomes and the parental CEF cells, miR-184-3p, miR-146a-3p, miR-146a-5p, miR-3538 and miR-155 expression was verified in both exosomes and CEF cells. In addition to miR-146a-5p, the qRT-PCR results in CEF cells of miR-184-3p, miR-146a-3p, miR-3538 and miR-155 were consistent with that in exosomes, suggesting that these miRNAs are key regulators in co-infection with ALV-J and REV.
In tumour progression, especially induced by viral synergistic infection, several studies have verified that miRNAs influence growth, differentiation, apoptosis, host-pathogen interactions, energy metabolism and other physiological processes [31–33]. While infecting cells, viruses integrate into the host genome to ensure viral persistence, which requires certain conditions for virus-vector interaction . Simultaneously, the viral replication also benefits the cell’s transcriptional and translational machinery, which may enhance the growth of the host cells. In addition, these regulations are based on energy metabolism [46, 47]. Our data suggested that the significantly differentially expressed miRNAs between co-infected and two single virus infected CEFs participated in energy metabolism, virus-vector interaction and cell growth, suggesting that these miRNAs are key regulators in co-infection with ALV-J and REV. These initial findings will lead to further exploration of the mechanism of ALV-J synergistic infection with REV.
We demonstrated that REV and ALV-J synergistically increased the accumulation of exosomal miRNAs. We revealed that a total of 54 and 16 miRNA genes were identified by comparing co-infection with two viruses with single-infected ALV-J and REV, respectively. These differentially expressed miRNAs participated in virus-vector interaction, oxidative phosphorylation, energy metabolism and cell growth, indicating potential new avenues to study the mechanism of synergistic infection of ALV-J and REV.
Cells and virus
DF-1 and chicken embryo fibroblasts (CEFs) cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% foetal bovine serum (FBS), 1% penicillin/streptomycin, and 1% l-glutamine, in a 5% CO2 incubator at 37°C. The stock SNV strain of REV at 103.2 50% tissue culture infectious doses (TCID50) and NX0101 strain of ALV-J at 103.8 TCID50 were maintained in our laboratory. The TCID50 of the SNV and NX0101 strains were titrated by limiting dilution in DF-1 culture.
Extraction of exosomes from CEF cells
The exosomes from Mock, single infection of ALV-J or REV, or co-infection of both ALV-J and REV CEFs were isolated using Total Exosome Isolation Reagent (Thermo Fisher Scientific) based on the manufacturer’s instructions.
Transmission electron microscopy
The protocol was conducted as described in a previous study .
CEF cells were lysed in cell lysis buffer (Beyotime) and incubated on ice for 5 minutes. ALV-J gp85, REV env expression, Hsp70, GRP78 and CD81 were detected by simple western analysis with anti-NX0101 gp85, anti-SNV env antibody, anti-Hsp70 (Bioss), anti-GRP78 (Bioss) antibody and anti-CD81 (Bioss) antibody at a 1:200, 1:200, 1:1000, 1:1000 and 1:1000 dilution, respectively.
Illumina small RNA deep sequencing
Total RNA of the infected CEF exosome samples was separated by 15% agarose gels to extract the small RNA (18-30 nt). After precipitation by ethanol and centrifugal enrichment of the small RNA population, the library was prepared according to the method and process of the Small RNA Sample Preparation Kit (Illumina, RS-200-0048). The RNA concentration of the library was measured using Qubit® RNA Assay Kit in Qubit® 2.0 to preliminarily quantify and then dilute to 1 ng/µl. The insert size was assessed using the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). The library with the expected insert size was then quantified accurately using TaqMan fluorescence probes of the AB Step One Plus Real-Time PCR system (library valid concentration >2 nM). The qualified libraries were sequenced by an Illumina HiSeq 2500 platform and 50 bp single-end reads were generated.
Real-time quantitative reverse transcription polymerase chain reaction
Primers used for real-time PCR
Size of PCR product
miRanda (http://www.microrna.org/microrna/) was used to predict targets of the differentially expressed miRNA. Gene enrichment and functional annotation analyses were conducted using Gene Ontology (GO; www.geneontology.org), Kyoto Encyclopedia of Genes and Genomes (KEGG, http://www.genome.jp/kegg), PATHWAY Database and LIGAND Database.
Results are presented as the mean ± standard deviation(s). The T test and one-way ANOVA test was performed using SPSS 13.0 statistical software. A P value less than 0.05 was considered statistically significant.
ZC conceived and designed the research. DZ wrote the manuscript. DZ and JW performed the experiments. SH, XD and JZ contributed reagents and materials. CL, LH, VN, and YY commented on the manuscript. All authors read and approved the final manuscript.
We are grateful to Ms. Pingping Zhuang, Dr. Guihua Wang, Ms. Li Zhang and the Annoroad Gene Technology Company for their technical assistance. We thank Dr. Gen Li and Dr. Mingjun Zhu for their helpful discussion and manuscript revision.
The authors declare no competing financial interests.
Availability of data and materials
The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.
Consent for publication
All authors consented to the publication of this manuscript. The funding agencies had no role in the decision to publish this manuscript.
Ethics approval and consent to participate
Approval for this study was obtained through the College of Veterinary Medicine of Shandong Agricultural University.
The study was supported by grants from the China-UK Partnership on Global Food Security: Combating tumour diseases for Sustainable Poultry Production (31761133002, BB/R012865/1), the Natural Science Foundation of China (31672521), the Shandong Modern Agricultural Technology & Industry System (SDAIT-11-04) and the Fund of Shandong “Double Tops” Program (2017).
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- Payne LN, Brown SR, Bumstead N, Howes K, Frazier JA, Thouless ME. A novel subgroup of exogenous avian leukosis virus in chickens. J Gen Virol. 1991;72(Pt 4):801–7.View ArticlePubMedGoogle Scholar
- Bai J, Payne LN, Skinner MA. HPRS-103 (exogenous avian leukosis virus, subgroup J) has an env gene related to those of endogenous elements EAV-0 and E51 and an E element found previously only in sarcoma viruses. J Virol. 1995;69:779–84.PubMedPubMed CentralGoogle Scholar
- Dong X, Ju S, Zhao P, Li Y, Meng F, Sun P, Cui Z. Synergetic effects of subgroup J avian leukosis virus and reticuloendotheliosis virus co-infection on growth retardation and immunosuppression in SPF chickens. Vet Microbiol. 2014;172:425–31.View ArticlePubMedGoogle Scholar
- Guo H, Li H, Cheng Z, Liu J, Cui Z. Co-Infection on immunologic function of T lymphocytes and histopathology in broiler chickens. J Integr Agric. 2010;09:1667–76.Google Scholar
- Cui Z, Sun S, Zhang Z, Meng S. Simultaneous endemic infections with subgroup J avian leukosis virus and reticuloendotheliosis virus in commercial and local breeds of chickens. Avian Pathol. 2009;38:443–8.View ArticlePubMedGoogle Scholar
- Davidson I, Borenstein R. Multiple infection of chickens and turkeys with avian oncogenic viruses: prevalence and molecular analysis. Acta Virol. 1999;43:136–42.PubMedGoogle Scholar
- Cheng Z, Zhang H, Wang G, Liu Q, Liu J, Guo H, Zhou E. Investigations of avian leukosis virus subgroup J and reticuloendotheliosis virus infections in broiler breeders in China. Isr J Vet Med. 2011;66:34–8.Google Scholar
- Harding C, Heuser J, Stahl P. Receptor-mediated endocytosis of transferrin and recycling of the transferrin receptor in rat reticulocytes. J Cell Biol. 1983;97:329–39.View ArticlePubMedGoogle Scholar
- Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S. Exosomes with immune modulatory features are present in human breast milk. J Immunol. 2007;179:1969–78.View ArticlePubMedGoogle Scholar
- Masyuk AI, Masyuk TV, Larusso NF. Exosomes in the pathogenesis, diagnostics and therapeutics of liver diseases. J Hepatol. 2013;59:621–5.View ArticlePubMedGoogle Scholar
- Vella LJ, Sharples RA, Nisbet RM, Cappai R, Hill AF. The role of exosomes in the processing of proteins associated with neurodegenerative diseases. Eur Biophys J. 2008;37:323–32.View ArticlePubMedGoogle Scholar
- Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN. Proteomic analysis of exosomes isolated from human malignant pleural effusions. Am J Respir Cell Mol Biol. 2004;31:114–21.View ArticlePubMedGoogle Scholar
- Schorey JS, Bhatnagar S. Exosome function: from tumor immunology to pathogen biology. Traffic. 2008;9:871–81.View ArticlePubMedPubMed CentralGoogle Scholar
- Beach A, Zhang HG, Ratajczak MZ, Kakar SS. Exosomes: an overview of biogenesis, composition and role in ovarian cancer. J Ovarian Res. 2014;7:14.View ArticlePubMedPubMed CentralGoogle Scholar
- El-Saghir J, Nassar F, Tawil N, El-Sabban M. ATL-derived exosomes modulate mesenchymal stem cells: potential role in leukemia progression. Retrovirology. 2016;13:73.View ArticlePubMedPubMed CentralGoogle Scholar
- Milane L, Singh A, Mattheolabakis G, Suresh M, Amiji MM. Exosome mediated communication within the tumor microenvironment. J Control Release. 2015;219:278–94.View ArticlePubMedGoogle Scholar
- Greening DW, Gopal SK, Xu R, Simpson RJ, Chen W. Exosomes and their roles in immune regulation and cancer. Semin Cell Dev Biol. 2015;40:72–81.View ArticlePubMedGoogle Scholar
- Narayanan A, Jaworski E, Duyne RV, Iordanskiy S, Guendel I, Das R, Currer R, Sampey G, Chung M, Kehn-Hall K. Exosomes derived from HTLV-1 infected cells contain the viral protein Tax. Retrovirology. 2014;11:O46.View ArticlePubMed CentralGoogle Scholar
- Selbach M, Schwanhausser B, Thierfelder N, Fang Z, Khanin R, Rajewsky N. Widespread changes in protein synthesis induced by microRNAs. Nature. 2008;455:58–63.View ArticlePubMedGoogle Scholar
- Baek D, Villen J, Shin C, Camargo FD, Gygi SP, Bartel DP. The impact of microRNAs on protein output. Nature. 2008;455:64–71.View ArticlePubMedPubMed CentralGoogle Scholar
- Shin VY, Chu KM. MiRNA as potential biomarkers and therapeutic targets for gastric cancer. World J Gastroenterol. 2014;20:10432–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Lytle JR, Yario TA, Steitz JA. Target mRNAs are repressed as efficiently by microRNA-binding sites in the 5’ UTR as in the 3’ UTR. Proc Natl Acad Sci USA. 2007;104:9667–72.View ArticlePubMedGoogle Scholar
- Towler BP, Jones CI, Newbury SF. Mechanisms of regulation of mature miRNAs. Biochem Soc Trans. 2015;43:1208–14.View ArticlePubMedGoogle Scholar
- Ambros V. MicroRNAs and developmental timing. Curr Opin Genet Dev. 2011;21:511–7.View ArticlePubMedPubMed CentralGoogle Scholar
- Blahna MT, Hata A. Regulation of miRNA biogenesis as an integrated component of growth factor signaling. Curr Opin Cell Biol. 2013;25:233–40.View ArticlePubMedPubMed CentralGoogle Scholar
- Subramanian S, Steer CJ. MicroRNAs as gatekeepers of apoptosis. J Cell Physiol. 2010;223:289–98.PubMedGoogle Scholar
- Zhou R, Rana TM. RNA-based mechanisms regulating host-virus interactions. Immunol Rev. 2013;253:97–111.View ArticlePubMedPubMed CentralGoogle Scholar
- Bhaskaran M, Mohan M. MicroRNAs: history, biogenesis, and their evolving role in animal development and disease. Vet Pathol. 2014;51:759–74.View ArticlePubMedGoogle Scholar
- Hartig SM, Hamilton MP, Bader DA, McGuire SE. The miRNA interactome in metabolic homeostasis. Trends Endocrinol Metab. 2015;26:733–45.View ArticlePubMedPubMed CentralGoogle Scholar
- Rosado J, Alvarez C, Clark D, Gotuzzo E, Talledo M. Differential miRNA expression profiles in Peruvian HTLV-1 carriers. Retrovirology. 2014;11:1.View ArticleGoogle Scholar
- Gupta P, Liu B, Wu JQ, Soriano V, Vispo E, Carroll AP, Goldie BJ, Cairns MJ, Saksena NK. Genome-wide mRNA and miRNA analysis of peripheral blood mononuclear cells (PBMC) reveals different miRNAs regulating HIV/HCV co-infection. Virology. 2014;450–451:336–49.View ArticlePubMedGoogle Scholar
- Janssen HL, Reesink HW, Lawitz EJ, Zeuzem S, Rodriguez-Torres M, Patel K, van der Meer AJ, Patick AK, Chen A, Zhou Y, et al. Treatment of HCV infection by targeting microRNA. N Engl J Med. 2013;368:1685–94.View ArticlePubMedGoogle Scholar
- Pilotti E, Casoli C, Bianchi MV, Bignami F, Prati F. MiRNA profile in CD4 positive T cells from HTLV-2 and HIV-1 mono- and co-infected subjects. Retrovirology. 2012;9:P2.View ArticlePubMed CentralGoogle Scholar
- Quinlan AR, Hall IM. BEDTools: a flexible suite of utilities for comparing genomic features. Bioinformatics. 2010;26:841–2.View ArticlePubMedPubMed CentralGoogle Scholar
- Cho JA, Yeo DJ, Son HY, Kim HW, Jung DS, Ko JK, Koh JS, Kim YN, Kim CW. Exosomes: a new delivery system for tumor antigens in cancer immunotherapy. Int J Cancer. 2005;114:613–22.View ArticlePubMedGoogle Scholar
- Hegmans JP, Bard MP, Hemmes A, Luider TM, Kleijmeer MJ, Prins JB, Zitvogel L, Burgers SA, Hoogsteden HC, Lambrecht BN. Proteomic analysis of exosomes secreted by human mesothelioma cells. Am J Pathol. 1807;2004:164.Google Scholar
- Lamparski HG, Metha-Damani A, Yao JY, Patel S, Hsu DH, Ruegg C, Le PJ. Production and characterization of clinical grade exosomes derived from dendritic cells. J Immunol Methods. 2002;270:211–26.View ArticlePubMedGoogle Scholar
- Schorey JS, Cheng Y, Singh PP, Smith VL. Exosomes and other extracellular vesicles in host-pathogen interactions. EMBO Rep. 2015;16:24–43.View ArticlePubMedGoogle Scholar
- Fleming A, Sampey G, Chung MC, Bailey C, van Hoek ML, Kashanchi F, Hakami RM. The carrying pigeons of the cell: exosomes and their role in infectious diseases caused by human pathogens. Pathog Dis. 2014;71:109–20.View ArticlePubMedGoogle Scholar
- Taylor DD, Zacharias W, Gerceltaylor C. Exosome isolation for proteomic analyses and RNA profiling. Methods Mol Biol. 2011;728:235.View ArticlePubMedGoogle Scholar
- Yang G, Zhang W, Yu C, Ren J, An Z. MicroRNA let-7: regulation, single nucleotide polymorphism, and therapy in lung cancer. J Cancer Res Ther. 2015;11(Suppl 1):C1–6.PubMedGoogle Scholar
- Minemura H, Takagi K, Miki Y, Shibahara Y, Nakagawa S, Ebata A, Watanabe M, Ishida T, Sasano H, Suzuki T. Abnormal expression of miR-1 in breast carcinoma as a potent prognostic factor. Cancer Sci. 2015;106:1642–50.View ArticlePubMedPubMed CentralGoogle Scholar
- Porrello ER, Johnson BA, Aurora AB, Simpson E, Nam YJ, Matkovich SJ, Dorn GW 2nd, van Rooij E, Olson EN. MiR-15 family regulates postnatal mitotic arrest of cardiomyocytes. Circ Res. 2011;109:670–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Pekarsky Y, Croce CM. Role of miR-15/16 in CLL. Cell Death Differ. 2015;22:6–11.View ArticlePubMedGoogle Scholar
- Kramer LD. Complexity of virus-vector interactions. Curr Opin Virol. 2016;21:81–6.View ArticlePubMedPubMed CentralGoogle Scholar
- Hirschey MD, DeBerardinis RJ, Diehl AME, Drew JE, Frezza C, Green MF, Jones LW, Ko YH, Le A, Lea MA, et al. Dysregulated metabolism contributes to oncogenesis. Semin Cancer Biol. 2015;35(Suppl):S129–50.View ArticlePubMedGoogle Scholar
- Martinez-Outschoorn UE, Lisanti MP, Sotgia F. Catabolic cancer-associated fibroblasts transfer energy and biomass to anabolic cancer cells, fueling tumor growth. Semin Cancer Biol. 2014;25:47–60.View ArticlePubMedGoogle Scholar
- Wang Y, Wang G, Wang Z, Zhang H, Zhang L, Cheng Z. Chicken biliary exosomes enhance CD4(+)T proliferation and inhibit ALV-J replication in liver. Biochem Cell Biol. 2014;92:145–51.View ArticlePubMedGoogle Scholar