Fig. 1

Model used to determine the effects of LRAs on LTR-driven transcription and splicing in the presence and absence of Tat. a Schematic of the in vitro model used in this study to determine the effects of LRAs on LTR-driven transcription and splicing. HEK293T cells were co-transfected with the LTR reporter construct together with a plasmid expressing Rev, with or without a plasmid expressing Tat for 48 h, followed by analysis using flow cytometry. The LTR construct expresses either unspliced protein fused to enhanced green fluorescent protein (EGFP) or spliced ∆38rev protein fused with DsRed. b Gating strategy of a representative sample used to identify the percentage of cells expressing EGFP or DsRed. c–e The combined results of 5 independent experiments showing the percentage of cells expressing EGFP (c), DsRed (d) or the percentage of spliced products (DsRed/DsRed+ EGFP) (e). The mean ± SEM of the 5 separate experiments, each run in triplicate, is shown. Comparisons were made to cells only (−) using a paired T test. Only statistically significant comparisons are shown **p < 0.01; ***p < 0.001