Uracil DNA glycosylase interacts with the p32 subunit of the replication protein A complex to modulate HIV-1 reverse transcription for optimal virus dissemination
© Herate et al. 2016
Received: 21 December 2015
Accepted: 27 March 2016
Published: 12 April 2016
Through incorporation into virus particles, the HIV-1 Vpr protein participates in the early steps of the virus life cycle by influencing the reverse transcription process. We previously showed that this positive impact on reverse transcription was related to Vpr binding to the uracil DNA glycosylase 2 enzyme (UNG2), leading to enhancement of virus infectivity in established CD4-positive cell lines via a nonenzymatic mechanism.
We report here that Vpr can form a trimolecular complex with UNG2 and the p32 subunit (RPA32) of the replication protein A (RPA) complex and we explore how these cellular proteins can influence virus replication and dissemination in the primary target cells of HIV-1, which express low levels of both proteins. Virus infectivity and replication in peripheral blood mononuclear cells and monocyte-derived macrophages (MDMs), as well as the efficiency of the viral DNA synthesis, were significantly reduced when viruses were produced from cells depleted of endogenous UNG2 or RPA32. Moreover, viruses produced in macrophages failed to replicate efficiently in UNG2- and RPA32-depleted T lymphocytes. Reciprocally, viruses produced in UNG2-depleted T cells did not replicate efficiently in MDMs confirming the positive role of UNG2 for virus dissemination.
Our data show the positive effect of UNG2 and RPA32 on the reverse transcription process leading to optimal virus replication and dissemination between the primary target cells of HIV-1.
Human immunodeficiency virus type 1 (HIV-1) has two main target cells, T CD4-positive lymphocytes and myeloid cells, including macrophages and dendritic cells. Infection of the CD4-positive T lymphocytes leads to the depletion of this cell population during the acute phase of the disease while macrophages are identified as one of the major reservoirs of virus . HIV-1-infected macrophages actively participate in virus dissemination and in the establishment of persistent virus reservoirs in different host tissues including lungs, gastro-intestinal and genital tracts, and the central nervous system (CNS) [2, 3]. Strains of HIV-1 have the ability to enter and infect different cell types in vitro and are usually subdivided into three main groups according to their co-receptor usage. Some viral strains use the beta-chemokine receptor CCR5 (R5 strains) and can infect monocytes and macrophages but also PBMCs and primary T lymphocytes. Other viral strains, emerging later during the infection, preferentially enter T lymphocytes and established T cell lines via the alpha-chemokine receptor CXCR4 (X4 strains) and are associated with progression to AIDS (for review, ). Dual tropic strains with both co-receptor usages also exist and can thus infect all the conventional HIV-1 target cells.
Like other viruses, HIV-1 utilizes or perturbs cellular pathways in order to optimize essential steps of the virus life cycle in T lymphocytes, macrophages or dendritic cells. Moreover, the HIV-1 genome contains additional genes encoding regulatory proteins Nef, Vif, Vpu and Vpr, which are viral factors specialized in hijacking and perturbing essential cellular pathways during virus replication through interactions with host cell proteins. However, Vpr is the only HIV-1 auxiliary protein specifically incorporated into virus particles through direct interaction with the Pr55Gag precursor, since its presence in the virion core will be subsequently required during the early steps of the virus life cycle in the newly infected cell (for review, ).
After virus entry, the viral core is released into the cytoplasm of the target cell where reverse transcriptase synthesizes the viral DNA, and the first role of Vpr is to influence the accuracy of the reverse transcription process leading to modulation of the mutation rate in the newly synthesized viral DNA. Vpr may also contribute to the mechanisms that allow the viral DNA to access the nuclear compartment. In addition, Vpr is a multifunctional protein that displays other activities including perturbations of the cell cycle progression, induction of apoptosis, and transcriptional modulation of host cell genes .
We and others have previously shown that the role of Vpr in the modulation of the reverse transcription process is related to the direct interaction and subsequent recruitment into virus particles of the nuclear form of uracil DNA glycosylase (UNG2) [6–9]. However, the specific role of UNG2 incorporation into virions was also challenged by other studies [10–12], suggesting that UNG2 had either a detrimental [11, 12] or a dispensable role in virus replication . UNG2 is a base excision repair enzyme mainly involved in removal of uracil residues from DNA at the replication fork during chromosome replication . The inclusion of uracil residues in DNA can occur either by mis-incorporation of dUTP during replication or by cytosine deamination. UNG2 is able to bind other key proteins of the DNA repair machinery such as the p32 subunit (RPA32) of the replication protein A complex (RPA), the proliferating cell nuclear antigen (PCNA) and the X-ray repair cross-complementing group 1 (XRCC1) [14–20]. The RPA complex is able to bind single-strand DNA (ssDNA) and can protect DNA intermediates during DNA replication and even stabilize DNA fragments caused by DNA damage [21, 22]. Interestingly, it was reported that the RPA complex promotes the recruitment of UNG2 at the replication fork [21, 22].
UNG2 has been also largely studied for its critical role in somatic hypermutation (SHM) and class-switch recombination (CSR) at the immunoglobulin locus of B lymphocytes . While controversial (for review, Refs. [13, 23, 24]), some reports showed that catalytically inactive mutants of UNG2 were fully proficient in CSR in B lymphocytes [25–27] suggesting that the specific function of UNG2 in CSR was not related to the catalytic activity of the protein but depends on a novel nonenzymatic function of UNG2. Similarly, we have recently reported that the catalytic activity of UNG2 is not required for modulation of the HIV-1 reverse transcription process and its positive impact on virus infectivity. This function of UNG2 is related to determinants located within the N-terminal region of the protein that is required for direct interaction with the RPA32 subunit of the RPA complex . Interestingly, it was recently reported that the RPA complex also played a specific role during CSR .
Here, we report results showing the positive impact of UNG2 and RPA32 on the reverse transcription leading to optimal virus infectivity, replication and dissemination between HIV-1 primary target cells. Interestingly, primary cells such as peripheral blood mononuclear cells (PBMCs) and monocyte-derived macrophages express low levels of these two cellular proteins.
Vpr, UNG2 and RPA32 can be associated together in a trimolecular complex
UNG2 and RPA32 are required for efficient HIV-1 replication in established human cell lines
As shown in Fig. 2b, c, the depletion of UNG2 in HeLa-CD4 cells led to a drastic decrease of virus replication as measured by the concentration of the viral p24 capsid protein (p24) in the cell-culture supernatant. This impairment in virus replication in shUNG2-transduced HeLa-CD4 cells (red curve and red bars, respectively) was observed as soon as 2 days post-infection and remained significant 4 and 8 days post-infection compared to shLuc-transduced HeLa-CD4 control cells (black curve and black bars). The requirement of the RPA32 protein for HIV-1 replication in HeLa-CD4 cells was similarly analyzed (Fig. 2b, c). Compared to control viruses produced in shLuc-transduced 293T cells and used to infect shLuc-transduced control HeLa-CD4 cells (black curve and black bars), viruses produced in RPA32-depleted cells also failed to replicate efficiently in RPA32-depleted HeLa-CD4 target cells (green curve and green bars). Together, these results clearly show the requirement of UNG2 and RPA32 proteins in both producing and target cells to ensure efficient virus replication. Furthermore, as previously reported , a significant decrease in virus infectivity, evaluated in a single-round infection assay with non-replicative GFP reporter viruses, was observed when viruses were produced in UNG2- and RPA32-depleted HeLa-CD4 cells (Fig. 2d), suggesting that incorporation of UNG2 and RPA32 into viral particles is required for maintaining full HIV-1 infectivity in this single-round infection assay. In order to confirm that the defect in virus replication in UNG2- and RPA32-depleted cells was related to a defect in the reverse transcription (RT) process, total viral DNA reverse transcripts were quantified 7 h after infection of HeLa-CD4 cells. As shown in Fig. 2e, a significant reduction in viral DNA synthesis was observed in UNG2- (red bar) and RPA32-depleted (green bar) cells compared to shLuc-transduced control cells (black bar).
UNG2 and RPA32 are expressed at low level in HIV-1 primary target cells
Impact of UNG2 and RPA32 on HIV-1 replication in PBMCs
Since we previously showed that the defect of virus replication in UNG2-depleted human CD4-positive cell lines (, Fig. 2e) was related to a defect in the RT process, we quantified total viral DNA reverse transcripts 7 h after infection of PBMCs with viruses produced in UNG2- or RPA32-depleted cells. As shown in Fig. 5c, a significant reduction of the total viral DNA was observed with viruses produced in UNG2-depleted cells (red bar) compared to viruses produced in control cells (black bar). Interestingly, a slight but significant decrease in viral DNA synthesis was also revealed when PBMCs were infected with viruses produced in RPA32-depleted cells (Fig. 5c, green bar). Together, these results confirm that UNG2 and RPA32 depletion negatively impacted virus replication in PBMCs by influencing the efficiency of the RT process. Again, production in UNG2- or RPA32-depleted cells had a negative impact on virus infectivity measured in a single-round infection assay using PBMCs as target cells (Fig. 5d) confirming the requirement for UNG2 and RPA32 for optimal virus infectivity.
UNG2 and RPA32 enhances HIV-1 replication in macrophages
As previously, we further investigated whether the impairment of replication in MDMs of UNG2- or -RPA32-depleted viruses was also linked to a RT defect during the establishment of infection. The total viral DNA reverse transcripts were measured 72 h after infection of MDMs with viruses produced from UNG2- or RPA32-depleted cells, and revealed a reduction of about 50–60 % of the total viral DNA synthesis compared to MDMs infected with control viruses (Fig. 6e). As observed in PBMCs, the absence of either UNG2 or RPA32 expression in virus-producing cells similarly decreases the efficiency of the RT process during viral replication in MDMs.
Finally, replication of viruses produced from cells depleted of both endogenous UNG2 and RPA32 proteins was evaluated in MDMs. 293T cells were thus co-transduced with two lentiviral vectors expressing specific shRNAs targeting UNG2 and RPA32, leading to efficient depletion of both proteins as evidenced by Western blot analysis (Fig. 6f). Replication-competent virus particles were produced in these double-depleted cells and used to challenge MDMs as previously. As shown in Fig. 6g, the replication impairment of viruses produced in cells depleted of both proteins (slanted red/green bar) was similar to the replication defect measured in MDMs infected with viruses produced in cells depleted of UNG2 only (red bar). The p24 concentration in MDM supernatant was indeed reduced of about 40–50 % for both viruses produced in shUNG2- or in shUNG2/shRPA32-tranduced cells 8 days after infection, showing that simultaneous depletion of UNG2 and RPA32 had no additional or synergistic effects compared to viruses produced in UNG2-depleted cells.
UNG2 impacts on HIV-1 dissemination between T cells and macrophages
Because macrophages express low levels of both UNG2 and RPA32 proteins, we developed experimental systems to analyze how UNG2 and RPA32 expression could influence cell-free virus dissemination from T lymphocytes to macrophages or, reciprocally, from macrophages to T lymphocytes.
Taken together, these results indicate that UNG2 is required for optimal replication and dissemination of cell-free virus particles between the target cells of HIV-1, while RPA32 is only required for dissemination from MDMs to target T cells.
We previously documented that incorporation of the UNG2 DNA repair protein into virus particles was required for modulation of the reverse transcription process [6, 30] resulting in a positive effect on HIV-1 infectivity and replication in established cell-lines . However, this positive impact of UNG2 on reverse transcription and virus replication was independent of the enzymatic activity of the UNG2 protein. Here, we report results showing that the HIV-1 Vpr protein can form a complex with UNG2 and the RPA32 subunit of the RPA complex in virus-producing cells, and participate in the modulation of the reverse transcription process for optimal virus replication and dissemination between the different target cells of HIV-1.
While several previous studies showed that UNG2 was efficiently recruited into HIV-1 virions [8–10, 12] indicating that this recruitment had a positive influence on viral replication [8, 9], the role of UNG2 incorporation was challenged by other studies, suggesting a dispensable [10, 29] or detrimental [11, 12, 31] effect of UNG2 on virus replication. Because all the previous studies regarding a detrimental or dispensable role of UNG2 in HIV-1 replication have been performed using established human cell-lines, we investigated and analyzed here the role of UNG2, and also RPA32, on virus replication and dissemination in HIV-1 primary target cells. In agreement with previous reports [8, 9, 32, 33], our data confirm the low levels of UNG2 and RPA32 expression in primary hematopoietic cells including PBMCs and MDMs, both at the protein and mRNA transcript levels, compared to established human cell-lines. Recent biochemical analyses performed in primary cells also confirmed that CD4-positive T cells and MDMs expressed modest and low levels of UNG activity, respectively . While the UNG2 and RPA32 proteins were not detectable in MDMs, expression in PBMCs seemed more variable and certainly higher than in MDMs. However, low but significant levels of UNG2 and RPA32 mRNAs were detected both in PBMCs and MDMs.
We now highlighted that UNG2 and RPA32 facilitated efficient virus replication and dissemination in PBMCs and macrophages. In these primary cells, the low level of UNG2 and RPA32 proteins did not allow the viruses to restore an efficient potential of replication. It was previously shown by Priet et al.  and Jones et al.  that UNG2 was required for efficient infection of macrophages with R5 viruses, but our data indicate that both X4 and R5 viruses replicated more efficiently in PBMCs and macrophages, respectively, when UNG2, and also RPA32, were expressed in virus-producing cells. These more efficient virus replication and dissemination were linked to a specific role of both UNG2 and RPA32 on reverse transcription, since we show here that a significant defect in the total viral DNA synthesis was systematically evidenced in these primary target cells when viruses were produced from UNG2- and RPA32-depleted cells. In addition, similar impairment of virus replication in macrophages was observed for viruses produced in cells simultaneously depleted of both cellular proteins, suggesting that these two proteins act in the same pathway for the control of HIV-1 replication and dissemination.
Differentiated macrophages residing in different host tissues, including the CNS and lymphoid tissues, are long-lived cell targets for productive viral replication. In order to analyze how UNG2 and RPA32 may influence dissemination of cell-free HIV-1 particles between macrophages and T lymphocytes, we developed experimental systems to show that viruses produced in macrophages, expressing low levels of UNG2 and RPA32, failed to replicate efficiently in UNG2- or RPA32-depleted Jurkat T cells. Similarly, viruses produced in UNG2-depleted Jurkat T cells did not replicate efficiently in MDMs confirming the positive role of UNG2 for dissemination of X4- and R5-tropic strains [8, 9]. While we failed to obtain substantial and sustain depletion of UNG2 and RPA32 in either MDMs or PBMCs in order to analyze their role for virus dissemination between HIV-1 primary target cells, our results suggest that UNG2 and RPA32, depending of the activation status of PBMCs and MDMs, may modulate virus spreading from and toward macrophages through positive regulation of the reverse transcription process. These observations suggest that UNG2 and RPA32 can thus contribute to virus dissemination and establishment of persistent reservoirs of virus-infected MDMs in different host tissues during the natural course of HIV-1 infection (, for review).
The determinants of UNG2 required for direct interaction with RPA32 [16, 18, 19, 34] are located in the same N-terminal region that the determinants involved in the modulation of the viral DNA mutation frequency . Since Vpr interacts directly with UNG2 through recognition of a WxxF motif found in the C-terminal part of the protein [6, 35], UNG2 might thus recruit Vpr and RPA32 simultaneously in a trimolecular complex (see Fig. 1d), as evidenced here in vitro using recombinant proteins but also with endogenous cellular proteins by co-immunoprecipitation assay. In addition, it would be interesting to analyze whether the two other subunits of RPA (i.e., RPA70 and RPA14) could also be recruited together with RPA32 in this complex.
The RPA complex plays key roles in different DNA repair pathways such as repair of DNA double-strand breaks by homologous recombination [36, 37] during post-replicative base excision repair (BER) in association with UNG2 [34, 38]. As a ssDNA binding protein, RPA32 is directly involved in the control of the assembly of the DNA repair machinery when DNA damage pathway signaling is engaged [39, 40]. Both RPA32 and UNG2 are thus present in the same replication foci , where the RPA complex recruits and enhances the ability of UNG2 to remove uracil in ssDNA at the replication fork [16, 18, 19, 34]. A similar involvement of both UNG2 and RPA32 proteins for efficient removal of uracil residues during viral DNA synthesis in primary HIV-1 target cells could be postulated. However, we previously showed that this positive impact of UNG2 on reverse transcription was related to a non-canonical scaffolding mechanism independent of the catalytic activity of the enzyme . Interestingly, several reports accumulated evidences for such a scaffolding nonenzymatic role of UNG2 and viral-related UNG proteins. While UNG2 is absolutely required for efficient CSR and controlled SHM processes in B lymphocytes, controversial results showing that the enzymatic removal activity of the protein is dispensable for these activities [23, 24, 26, 41, 42]. It was suggested that the N-terminal domain of UNG2 may recognize DNA double-strand breaks and acts as an accessory site to provide a structural support for a scaffold function of the protein [27, 43]. Such a scaffolding function of UNG2, independent of its enzymatic activity but related to the N-terminal part of the protein, was also highlighted by its role during the assembly of the human centromere protein A (CENP-A) to sites of DNA damage . Interestingly, CSR and assembly of CENP-A are both inhibited by ectopic expression of the wild-type HIV-1 Vpr protein via direct interaction with UNG2 suggesting that UNG2 may be recruited or act through its WxxF motif during these processes [26, 44].
Furthermore, UNG proteins encoded by numerous viruses, such as Poxviridae and Herpesviridae, are required for efficient virus replication in their respective primary target cells through a mechanism independent of the uracil-excising activity of their UNG protein [45–48]. For example, it was recently showed that the BKRF3 UNG protein encoded by Epstein–Barr virus played a critical role in the viral DNA synthesis by recruiting cellular and viral proteins to replication sites, but this function was also independent of its enzymatic activity . While we cannot formally exclude that UNG2 also acts on the HIV-1 reverse transcription process through an enzymatic-dependent mechanism, our results highlight a potential scaffolding role of UNG2 related to RPA32 binding. This nonenzymatic scaffolding function of cellular UNG2 would be important during HIV-1 dissemination. Nonetheless, further investigations should give access to the characterization of this novel role of UNG-related proteins in various biological activities such as CSR and SHM processes in B lymphocytes as well as DNA synthesis of several viruses, including HIV-1.
Our results strongly suggest that UNG2 acts as a scaffold protein recruiting RPA32 into virions and that this interaction is required during the UNG2-dependent mechanism of RT control. Interestingly, it was very well documented that the RPA trimeric complex directly participated and played a crucial role in DNA replication of the simian virus 40 (SV40) and other polyomaviruses (, for review). More recent reports showed that this protein complex was also involved, indirectly or directly through viral DNA binding, in DNA synthesis of numerous other viruses, including Eptein Barr and Herpes simplex herpesviridae, adenovirus as well as polyoma- and papillomaviruses [45–48, 50–52]. During SV40 replication, RPA must act at different steps including the opening of the double strand DNA and the stabilization of the single strand DNA . Although the exact mechanism of the RPA complex during the HIV-1 DNA synthesis needs to be specifically determined, we can hypothesize that RPA allows both (1) for efficient recruitment and processivity of the viral RT, and (2) for protection of the single strand viral DNA from degradation by direct binding. Indeed, it was also reported that the RPA complex was able to inhibit the deamination activity and the processivity of the APOBEC3G cytidine deaminase, suggesting that RPA plays a role in DNA protection from the editing activity of APOBEC3 proteins . This last observation could support a model in which the recruitment of RPA32 by Vpr and UNG2 at the site of RT leads to the binding of RPA32 to the negative viral DNA for protection from deamination and nuclease activities. However, this protective role of RPA32, particularly against APOBEC3G activity, has to be more explored and might explain why the presence of Vpr can limit the APOBEC3G effect as reported before . In agreement with a role of the RPA32 subunit of the RPA trimeric complex in HIV-1 DNA synthesis and viral replication, it was also previously shown that the RPA complex had a positive impact, at least in vitro, on the efficiency of the HIV-1 reverse transcription [54, 55].
The results reported here indicate that cellular UNG2 and RPA32 facilitate optimal virus replication and dissemination in PBMCs and macrophages through positive modulation of the reverse transcription process. Interestingly, and in favor of a positive impact of the endogenous UNG2 for virus replication, it has been recently shown that the DNA synthesis of the human hepatitis B virus (HBV) was also facilitated in a UNG2-dependent manner in primary hepatocytes [56, 57]. In this model, UNG2 may counteract APOBEC-induced hypermutation of the HBV genome. Similarly, our results argue for a positive role of UNG2 and RPA for optimal viral DNA synthesis and virus dissemination between the primary target cells of HIV-1. These cellular proteins may thus contribute to virus dissemination and the establishment of viral reservoirs in different host tissues during the natural course of HIV-1 infection.
Vectors and expression plasmids
Vectors for expression of the HA-tagged forms of the wild-type UNG2 and Vpr proteins have been described previously [7, 58]. For the plasmid encoding HA-tagged RPA32, the RPA32 coding sequence was amplified by PCR with specific primers from the pGAD-RPA32 plasmid described previously , and the PCR product was then subcloned into the BamHI and XhoI restriction sites of the pAS1B plasmid as described . The plasmid for expression in bacteria of UNG2 fused to GST has been described previously , while the plasmid encoding for GST-RPA32 (pGEX4T1-GST-RPA32) has been kindly provided by Yuan Jingsong from the University of Texas (USA). The pLKO.1 lentiviral vectors containing the gene for puromycine resistance and harboring shRNA targeting UNG2, RPA32 or the firefly luciferase (Luc) control were purchased from Sigma and have been described . The pLKO.1-shLuc-GFP, and the pLKO.1-shRPA32-GFP vectors were constructed by replacing the puromycin resistance gene by the GFP reporter gene between the BamHI and the KpnI restriction sites of the parental pLKO.1-shUNG2 and -shRPA32 vectors . The infectious clones of the NL4.3 and YU2 HIV-1 isolates (pNL4.3 and pYU2), as well as the plasmid encoding the VSV-G envelope glycoprotein, have been described [6, 7, 30].
Cell culture and transfection
Jurkat T cells were maintained in Roswell Park Memorial Institute Medium (RPMI) while 293T cells and HeLa cells stably expressing CD4 (HeLa-CD4 cells) were grown in Dulbecco Minimal Essential Medium supplemented with 10 % fetal calf serum (FCS), 100 IU penicillin/mL and 100 µg streptomycin/mL (Invitrogen); shRNA-transduced Jurkat and 293T cells were maintained in complete medium containing 1 µg/mL puromycin (Invitrogen). Human monocytes and PBMCs were isolated from blood of healthy volunteers (Etablissement Français du Sang, Hôpital Saint-Antoine, Paris, France) by density gradient sedimentation in Ficoll (GE Healthcare) followed by adhesion-selection for 2 h at 37 °C. After extensive washing, monocytes were differentiated in macrophages (MDMs) for 10 days in complete culture medium RPMI 1640 supplemented with 20 % FCS, 100 IU penicillin/mL, 100 µg streptomycin/mL (Invitrogen) and 10 ng/mL of macrophage colony-stimulating factor (M-CSF) (Miltenyi Biotec). For activation of PBMCs, cells were grown in complete RPMI medium supplemented with phytohemagglutinin (PHA) (5 µg/mL) for 72 h and then resuspended in complete medium containing 10 ng/mL of IL-2. All cells were grown at 37 °C under 5 % CO2. For virus production, immunoprecipitation and pulldown assays, 293T cells were transfected using calcium phosphate DNA precipitation technique as described [6, 7, 59]. For lentiviral vector production, 293T cells were transfected using Jet Pei reagent (Polyplus Transfection) according to the manufacturer’s instructions.
Pulldown and immunoprecipitation assays, and immunoblot analysis
The pulldown assay for analyzing interactions between UNG2, RPA32 and Vpr was performed as previously described [6, 60]. Briefly, GST-UNG2, GST-RPA32 and GST were produced in E. coli, strain BL21, as described . 5 µg of recombinant GST, GST-UNG2 or GST-RPA32 were immobilized on 15 µL of GSH-Sepharose beads (GE Healthcare). Beads were washed and then incubated with 500 µg of lysate from transfected 293T cells as described previously [60, 61]. Bound material was resolved by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) on 12 % acrylamide precast gels (BioRad) and then analyzed by Western blotting with anti-HA (3F10, Roche) and anti-β-actin (Sigma) antibodies.
For immunoprecipitation assay, 293T cells expressing HA-Vpr were lysed as described previously . After quantification with Bradford (Bio-Rad), 500 µg of cell lysate proteins were incubated with 30 µL of anti-HA affinity matrice (clone 3F10, Roche) for 2 h under gentle shaking at 4 °C. Elution from beads was carried out by incubation in 30 µL of 1× Laemmli buffer containing DTT for 10 min at 95 °C. 20 µg of the protein lysates and 15 µL of the precipitate were then resolved by SDS-PAGE on 12 % acrylamide precast gels (BioRad). Immunoprecipitate and cell lysate proteins were then analyzed by Western blotting with anti-HA (3F10, Roche), anti-UNG2 (clone 2C12, Origene), anti-RPA32 (clone RPA3-19, Abcam) and anti-β-actin (Sigma) antibodies.
For analysis of endogenous UNG2 and RPA32 expression in the different cell-lines and primary cells, as well as in transduced cells, cells were lysed using a NP40 buffer containing a protease inhibitor (Roche) by incubation for 30 min on a wheel at 4 °C. After spinning, supernatant was collected and the concentration of proteins was quantified by Bradford analysis using the manufacturer’s protocol (BioRad). Cell lysates were then resolved by SDS-PAGE and analyzed by Western blotting using anti-UNG2 (clone 2C12, Origene), anti-RPA32 (clone RPA3-19, Abcam) and anti-β-actin (Sigma) antibodies.
Quantification of UNG2 and RPA32 mRNA by qRT-PCR analysis
RNA of cell lines and primary cells was extracted using the Pure link RNA mini kit (Ambion) according to manufacturer’s instructions. The reverse transcription was performed on equal amount of mRNA using the Maxima reverse transcriptase (Thermo Scientific) according to the manufacturer’s instructions. The RT reaction was processed as follow: 10 min at 25 °C, 15 min at 50 °C and 5 min at 85 °C. Then, UNG2 and RPA32 cDNAs were quantified by the LightCycler 480 qPCR system (Roche Applied Science). For UNG2 cDNA amplification, we used as forward primer 5′-GCCAGAAGACGCTCTACTCC-3′ and as reverse primer 5′-GCATCTCCGCTTTCCTCA-3′ which are specific for UNG2 and do not amplify UNG1. For RPA32 cDNA amplification, we used as forward primer 5′-AGGCCACCTGAGATCTTTTC-3′ and as reverse primer 5′-GGCTTTGCTTAGTACCATGTG-3′. Each PCR reaction contains 1X SYBR Green I Master mix (Roche), 100 nM of each primer and 1 µL of the RT product. For absolute quantification, we used dilutions of genomic DNA which contains known copies of genome and used as an internal control. Results were expressed as the percentage of UNG2 and RPA32 mRNA copies relative to those measured from 293T cell RNA extract.
UNG2-, RPA32- and UNG2/RPA32-depleted cells
VSV-G-pseudotyped lentiviral particles (LVPs) harboring shRNA targeting Luc (shLuc), UNG2 (shUNG2), or RPA32 (shRPA32) were produced in 293T cells as described previously . LVPs were then used to transduce 293T, HeLa-CD4 or Jurkat cells, and the levels of UNG2 or RPA32 protein expression were assessed by Western blot as previously . For double-depletion of UNG2 and RPA32, 293T cells were first transduced with LVPs harboring the shRNA targeting UNG2 and the puromycin resistance gene. After selection in cell culture medium containing puromycin as described previously , shUNG2-tranduced cells were transduced with LVPs harboring the shRNA targeting RPA32 and the GFP encoding sequence, and GFP-positive cells were sorted 72 h later using the BD FACSJAZZ cell sorter.
Virus production, replication and infectivity assays
Replication-competent HIV-1 (NL4.3 or YU2 strains) were produced as previously described in shRNA-transduced 293T cells by transfection with pNL4.3 or pYU2 molecular clones , and the plasmid for expression VSV-G was added to the DNA mixture when indicated. For HIV-1 replication monitoring, HeLa-CD4 cells (2 × 105), Jurkat cells (3 × 105), MDMs (1 × 106) or PHA/IL-2-activated PBMCs (2 × 106) were seeded and infected in 6-well plates with 200 ng of viral p24. Cell culture supernatants were then collected 2, 4, and 8 days after infection (depending on the experiments) for p24 concentration measurement by enzyme-linked immunosorbent assays (ELISA) as described . To monitor HIV-1 infectivity, single-round-infection viruses carrying the GFP gene were produced as previously described  in shRNA-transduced 293T cells followed by transfection with a DNA mix containing the HIV-1-packaging plasmid (pCMVDR8.2), the HIV-1 vector encoding GFP (pHIvec2.GFP), the plasmid encoding the HIV-1 envelope glycoproteins of the YU-2 isolate. The supernatant was then collected, filtered, and ultracentrifuged to pellet viruses as described previously . For single-round infection experiments, HeLa-CD4 cells (2 × 105), Jurkat cells (3 × 105), MDMs (1 × 106) or PHA/IL-2-activated PBMCs (2 × 106) were seeded and infected in 6-well plates with 500 ng of p24. Cells were cultured at 37 °C for 60 h and samples were then fixed in 1 % paraformaldehyde (Sigma-Aldrich) and data were collected on a Cytomix FC500 cytometer (Beckman-Coulter). The percentage of GFP-positive cells was analyzed using the RXP analysis software. Viral infectivity was calculated by normalizing the percentage of GFP-positive cells to that obtained in cells infected with viruses produced in shLuc-transduced 293T cells.
Quantification of total viral DNA reverse transcripts
One day prior to infection, HeLa-CD4 cells (2 × 105), Jurkat cells (2 × 105), MDMs (1 × 106) or activated PBMCs (2 × 106) were plated in 6-well plates. Before infection, replication-competent viruses were incubated with DNAseI (Roche) for 1 h at 37 °C, and 0.5 µg (or 1 µg for PBMCs) of p24 was then used for infection. 3 h after infection (or 24 h for MDMs), viruses were washed off and the cells were subsequently incubated at 37 °C in complete medium supplemented with 0.5 µM saquinavir in order to restrict viral replication to a single cycle. For HeLa-CD4, Jurkat cells and PBMCs, cell samples were collected 7 h after infection and DNA was extracted using the QIAamp DNA Blood Mini Kit (Qiagen) according to the manufacturer’s protocol. For MDMs, DNA extraction was carried out 72 h after infection as described . The total level of HIV-1 DNA reverse-transcripts was quantified via the LightCycler 480 qPCR system (Roche Applied Science) as previously described [7, 63]. Briefly, the quantitative PCR for total HIV-1 DNA was carried out using primers targeting the U5-gag region within the HIV-1 genomic sequence in a 10-µL final volume consisting of 2X FastStartDNA Tag polymerase (Roche) and 0.3 µM of sense MH532 (5′-TGTGTGCCCGTCTGTTGTGT-3′) and antisense MH531 (5′-GAGTCCTGCGTCGAGAG ATC-3′) primers (TIB MolBiol). The fluorescent probe primers 5′-LC640-TCTCTAGCAGT GGCGCCCGAACAG-PH and 5′-CCCTCAGACCCTTTTAGTCAGTGTGGAA-FL were used at a concentration of 0.2 µM. Total DNA was expressed as copy numbers per cell, with the DNA template normalized by β-globin gene amplification using a LightCycler control kit DNA (Roche).
CH, CV, CAG, and SB designed research; CH, CV, CAG, ML and MCR performed experiments; CH, CV, CAG, ML, MCR and SB analyzed data; CH and SB wrote the paper. All authors read and approved the final manuscript.
We thank Yuan Jingsong from the University of Texas (Houston, USA) for the kind gift of reagents. We thank the members of the Flow Cytometry (Cybio) and the Genomic core facilities of the Cochin Institute for their technical help. This work was supported in part by INSERM, the CNRS and the University Paris-Descartes. It is also funded by Grants from the Agence Nationale de Recherche sur le SIDA et les Hépatites virales (ANRS) and Sidaction. C.H. was supported by a Grant from the French ministry of research.
The authors declare that they have no competing interests.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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