Modulation of human endogenous retrovirus (HERV) transcription during persistent and de novo HIV-1 infection

Retrovirology

Table 3 Time course of HERV-K (HML-2) transcription in HIV-1 infected LC5-RIC cells

Provirus ^a	Chromosome band	Location of amplicon in genome ^b	Number of clones (% cloning frequency)
			Day-0	Day-3	Day-13	Day-22
ERVK-7^c (c1_B, K102, K50a)	1q22	153870020–153870613	4 (13.3)	9 (23.1)	3 (9.4)	6 (16.7)
ERVK-2 (c3_A, K11)	3p25.3	9869046–9869639	1 (3.3)	-	-	-
ERVK-3^c,d (c3_B, K106, K68)	3q13.2	114232740–114233333	14 (46.7)	2 (5.1)	3 (9.4)	5 (13.8)
ERVK-4^c (c3_C, K (I))	3q21.2	127093474–127094067	-	-	2 (6.3)	5 (13.8)
ERVK-6^c,d (c7_A, K (HML-2. HOM), K108)	7p22.1	4604296–4604932	-	-	-	1 (2.8)
ERVK-6^c,d (c7_A, K (HML-2. HOM), K108)	7p22.1	4595814–4596407^e
ERVK-15 (c7_C, K (OLDAC004979))	7q34	141101009–141101702	8 (26.7)	25 (64.1)	19 (59.4)	19 (52.8)
ERVK-16 (c10_A, M3.8)	10p14	6913372–6913968	3 (10.0)	2 (5.1)	4 (12.5)	-
ERVK-17^c (c10_B)	10q24.2	101576433–101577026	-	1 (2.6)	1 (3.1)	-
Total number o f clones			30 (100)	39 (100)	32 (100)	36 (100)

^aDesignation according to [87], aliases are in parentheses.
^bAccording to the March 2006 assembly (NCBI36/hg18) of the human genome sequence at the Human Genome Browser [88].
^cHuman-specific HERV-K (HML-2) proviruses [89].
^dPolymorphic in the human genome [89].
^eThe human reference genome sequence harbors a tandem provirus [90]. Due to the lack of sufficient sequence differences the detected cDNA sequence may stem from one or the other provirus.

ISSN: 1742-4690